Article
Biochemical Research Methods
Ragini Pant, Amit Joshi, Tanuja Joshi, Priyanka Maiti, Mahesha Nand, Tushar Joshi, Veena Pande, Subhash Chandra
Summary: Recent research suggests that lichen compounds may serve as potential therapeutic agents for treating multi-drug resistant tuberculosis; virtual screening and molecular dynamics simulation identified Portentol, Aspicilin, Parietinic acid, and Polyporic acid as potential inhibitors of Ag85C.
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2021)
Article
Chemistry, Physical
Rahul Singh, Sachin Kumar, Vijay Kumar Bhardwaj, Rituraj Purohit
Summary: This study analyzed the interaction ability of aminoarylbenzosuberene (AAB) molecules with DprE1 and identified a promising molecule (AAB4) as a potential lead against tuberculosis. AAB4 showed better inhibition potency and stability compared to co-crystallized inhibitors and also inhibited a drug-resistant mutant. These findings suggest the potential of AAB4 as a therapeutic option for tuberculosis.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Review
Chemistry, Medicinal
Yashodeep Shinde, Iqrar Ahmad, Sanjay Surana, Harun Patel
Summary: Tuberculosis transmitted by Mycobacterium tuberculosis is a global concern, with drug resistance posing a significant challenge. Targeting the Mtb cell wall peptidoglycan synthesis is an attractive strategy to combat drug resistance, with Mur enzymes serving as potential targets.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Infectious Diseases
Eddie Samuneti Solo, Yasuhiko Suzuki, Trevor Kaile, Precious Bwalya, Patrick Lungu, Joseph Yamweka Chizimu, Yogendra Shah, Chie Nakajima
Summary: The study found that the LAM family was the most predominant genotype among the 274 isolates in Lusaka, followed by the CAS family and the T family. Some subfamilies showed a propensity for multidrug-resistant tuberculosis (MDR-TB).
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Review
Pharmacology & Pharmacy
Taher Azimi, Saeed Khoshnood, Arezoo Asadi, Mohsen Heidary, Hassan Mahmoudi, Vahab Hassan Kaviar, Masoume Hallajzadeh, Mohammad Javad Nasiri
Summary: This study investigated the prevalence of LNZ resistance among MDR-TB isolates through systematic review and meta-analysis, revealing an increasing trend of LNZ resistance in MDR-TB cases. It is recommended to combine linezolid with newer anti-TB drugs to enhance effectiveness and reduce toxicities in the long-term treatment of MDR-TB cases.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Medicine, General & Internal
Sanghee Park, Jihee Jung, Jiyeon Kim, Sang Bong Han, Sungweon Ryoo
Summary: This study aimed to investigate the resistance and genetic mutations of drug-resistant Mycobacterium tuberculosis (DR-Mtb) isolates to clofazimine (CFZ), in order to improve the diagnosis and treatment of drug-resistant tuberculosis (TB) patients. The results showed that the rate of CFZ resistance in drug-resistant Mtb isolates was 4.1%, and it was not related to treatment with CFZ or bedaquiline. Four novel mutation sites related to CFZ resistance were identified in this study.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Infectious Diseases
Precious Bwalya, Tomoyuki Yamaguchi, Eddie Samuneti Solo, Joseph Yamweka Chizimu, Grace Mbulo, Chie Nakajima, Yasuhiko Suzuki
Summary: This study analyzed mutations in MDR-TB isolates in Zambia and showed the relationship between resistance and genotypes. The most common mutations were found in rpsL, rrs, and gidB, with specific genotypes strongly associated with resistance. The results suggest that rapid genetic testing for STR resistance is recommended before treatment in Zambia.
Article
Immunology
Mariateresa Coppola, Rachel P-J. Lai, Robert J. Wilkinson, Tom H. M. Ottenhoff
Summary: This study fills the knowledge gap regarding pulmonary expression of Mtb genes, revealing high concordance in the in vivo expressed genes among different datasets and species. It suggests that the consistently and highly expressed genes in vivo could be novel targets for future TB vaccine and drug development.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Jamal El Bakali, Michal Blaszczyk, Joanna C. Evans, Jennifer A. Boland, William J. McCarthy, Imam Fathoni, Marcio V. B. Dias, Eachan O. Johnson, Anthony G. Coyne, Valerie Mizrahi, Tom L. Blundell, Chris Abell, Christina Spry
Summary: By performing a fragment screen, we identified three series of fragments that occupy distinct regions in the active site of MtbPPAT. Guided by X-ray crystal structures, we successfully linked weakly-binding fragments to produce an active site binder with Mtb activity, as demonstrated by CRISPR interference. This study represents a significant progress in validating MtbPPAT as a potential drug target and designing anti-TB drugs targeting MtbPPAT.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Pooja Singh, Salma Jamal, Faraz Ahmed, Najumu Saqib, Seema Mehra, Waseem Ali, Deodutta Roy, Nasreen Z. Ehtesham, Seyed E. Hasnain
Summary: This study aims to develop an updated database including almost all reported substitutions within Mycobacterium tuberculosis drug target genes and use bioinformatics tools to evaluate the impact of these mutations. They have created a comprehensive online resource named MycoTRAP-DB that connects gene mutations with structural, functional, and pathogenic implications. This integrated platform allows for comprehensive analysis and prioritization of SNPs for the development of improved antimycobacterial medications.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Adeniyi T. Adewumi, Ahmed Elrashedy, Opeyemi S. Soremekun, Mary B. Ajadi, Mahmoud E. S. Soliman
Summary: This study investigated the structural features and dynamics of the Ag85C protein in Mycobacterium tuberculosis, and identified potential targets for antitubercular drugs. The findings have important implications for the design of novel compounds with potent anti-TB activity.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Chemistry, Organic
Jeffrey Ash, Hai Huang, Paula Cordero, Jun Yong Kang
Summary: A method has been developed for the synthesis of elusive phosphonic, phosphinic, and phosphoric monoacids that involves the activation of inert pentavalent P(v)-compounds to form a reactive phosphoryl pyridinium intermediate, which in turn reacts with H2O to selectively deprotect one alkoxy group and form organophosphorus monoacids.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Environmental Sciences
Liu Chun Fa, Song Yi Meng, He Ping, Liu Dong Xin, He Wen Cong, Li Yan Ming, Zhao Yan Lin
Summary: MDR-LAMP assay showed high sensitivity and specificity in early diagnosis of multidrug-resistant tuberculosis, with good predictive performance in detecting resistance to isoniazid and rifampicin, as well as mutations in rpoB, katG, and inhA genes.
BIOMEDICAL AND ENVIRONMENTAL SCIENCES
(2021)
Article
Infectious Diseases
Matthias Merker, Nkongho F. Egbe, Yannick R. Ngangue, Comfort Vuchas, Thomas A. Kohl, Viola Dreyer, Christopher Kuaban, Jurgen Noeske, Stefan Niemann, Melissa S. Sander
Summary: The study in Cameroon revealed that pre-existing resistance to high-dose isoniazid and ethambutol is associated with recent transmission of RR/MDR strains. Additionally, individuals aged between 30 and 50 were more likely to be involved in recent transmission. The lack of universal drug susceptibility testing in Cameroon may result in prolonged exposure of new RR/MDR-TB patients to sub-optimal or failing first-line drug regimens.
BMC INFECTIOUS DISEASES
(2021)
Article
Biotechnology & Applied Microbiology
Kelvin W. C. S. Leong, Sanjay S. Gautam, Manoj Pradhan, Y. Ibotomba K. Singh, K. C. R. Rajendra, Sagar Rajbhandari, Gokarna R. Ghimire, Krishna Adhikari, Uma Shrestha, Raina Chaudhary, Gyanendra Ghimire, Sundar Khadka, Ronan F. O'Toole
Summary: Nepal has a comparable tuberculosis incidence rate to its neighboring high incidence countries, and it also sees a significant number of multi-drug resistant tuberculosis cases each year. This study analyzed the whole-genome of multi-drug resistant tuberculosis isolates from Nepal and identified specific mutations related to their resistance. The study also found a high proportion of fluoroquinolone resistance among the Nepal isolates. These findings have important implications for the management of tuberculosis in Nepal.
Article
Microbiology
Thulasi Warrier, Marielle Tropis, Jim Werngren, Anne Diehl, Martin Gengenbacher, Brigitte Schlegel, Markus Schade, Hartmut Oschkinat, Mamadou Daffe, Sven Hoffner, Ali Nasser Eddine, Stefan H. E. Kaufmann
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2012)
Article
Chemistry, Medicinal
Carolyn Vargas, Gerald Radziwill, Gerd Krause, Anne Diehl, Sandro Keller, Nestor Kamdem, Constantin Czekelius, Annika Kreuchwig, Peter Schmieder, Declan Doyle, Karin Moelling, Volker Hagen, Markus Schade, Hartmut Oschkinat
Article
Chemistry, Multidisciplinary
Chris Phillips, Lee R. Roberts, Markus Schade, Richard Bazin, Andrew Bent, Nichola L. Davies, Rob Moore, Andrew D. Pannifer, Andrew R. Pickford, Stephen H. Prior, Christopher M. Read, Andrew Scott, David G. Brown, Bin Xu, Stephen L. Irving
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2011)
Article
Multidisciplinary Sciences
Christoph Scheich, Zoltan Szabadka, Beata Vertessy, Vera Puetter, Vince Grolmusz, Markus Schade
Article
Chemistry, Medicinal
Jo Alen, Markus Schade, Markus Wagener, Frank Christian, Sonja Nordhoff, Beatrix Merla, Torsten R. Dunkern, Gregor Bahrenberg, Paul Ratcliffe
JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Chemistry, Medicinal
Markus Schade, Beatrix Merla, Bernhard Lesch, Markus Wagener, Simone Timmermanns, Katrien Pletinckx, Torsten Hertrampf
JOURNAL OF MEDICINAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Andrea Bertarello, Pierrick Berruyer, Markus Artelsmair, Charles S. Elmore, Sepideh Heydarkhan-Hagvall, Markus Schade, Elisabetta Chiarparin, Staffan Schantz, Lyndon Emsley
Summary: Determination of intracellular drug concentrations is crucial for understanding drug function and efficacy, but currently there is no experimental technique that satisfies the requirements of nondestructive measurement, no modification of drugs or targets, and detection of low concentrations. In this study, the use of magic-angle spinning dynamic nuclear polarization technique is proposed to fulfill these requirements, and successful estimation of intracellular drug amounts is demonstrated for the first time.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Pharmacology & Pharmacy
M Schade
Review
Pharmacology & Pharmacy
M Schade, H Oschkinat
CURRENT OPINION IN DRUG DISCOVERY & DEVELOPMENT
(2005)
Article
Multidisciplinary Sciences
JD Kahmann, DA Wecking, V Putter, K Lowenhaupt, YG Kim, P Schmieder, H Oschkinat, A Rich, M Schade
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2004)
Article
Chemistry, Multidisciplinary
Mangesh Joshi, Carolyn Vargas, Prisca Boisguerin, Annette Diehl, Gerd Krause, Peter Schmieder, Karin Moelling, Volker Hagen, Markus Schade, Hartmut Oschkinat
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2006)