期刊
JOURNAL OF MEDICAL VIROLOGY
卷 80, 期 10, 页码 1740-1746出版社
WILEY-BLACKWELL
DOI: 10.1002/jmv.21287
关键词
HCV; genotype; sequence
类别
资金
- Natural Science Foundation of China (NSFC) [30460119]
- International Collaboration Project [2006GH07]
- Natural Science and Application Project (Yunnan Provincial Government, China) [2007C038M]
Full length genome sequences were characterized for three novel hepatitis C virus (HCV) isolates (here named DH012, DH014, and DH028). The complete genomes were all isolated from injecting drug users (IDUs) who were co-infected with HIV-1 and lived in Dehong prefecture, Yunnan Province, China, which neighbors Myanmar. The three genomes are 9,443-9,470 nt in length and each contains a single open reading frame (ORF) of 9,069 nt long. Pairwise comparisons indicated nucleotide similarities of 97.9-98.6% among the three isolates, and similarities of 72.4-75.0% between the three isolates and 20 reference strains (representing HCV subtypes 6a-6q and 6t plus two unassigned genotype 6 isolates km41 and gz52557). Phylogenetic analyses demostrated that the three isolates formed a tight and well-supported monophyletic cluster in the genotype 6 clade. No evidence of viral recombination was found using similarity plots and bootscanning analyses. Based on the current HCV classification criteria, we have assigned the three isolates to a new subtype, 6u. Although another 6u isolate D83 has been reported very recently, it is subtypically distinct from the three isolates we described here. Because its designation does not meet the criteria described in the updated HCV nomenclature proposal, this 6u isolate should be reclassified.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据