期刊
JOURNAL OF MEDICAL PRIMATOLOGY
卷 38, 期 5, 页码 360-370出版社
WILEY
DOI: 10.1111/j.1600-0684.2009.00361.x
关键词
Cercocebus atys; DGGE; intestinal hydrogenotrophs; Papio hamadryas; quantitative PCR
资金
- Yerkes National Primate Research Center [NIH RR-00165]
- Southwest Foundation for Biomedical Research
- Southwest National Primate Research Center
- NSF [9707361]
- General Mills Bell Institute of Health and Nutrition
- Division Of Behavioral and Cognitive Sci
- Direct For Social, Behav & Economic Scie [9707361] Funding Source: National Science Foundation
Background Methanogenesis by methanogenic Archaea and sulfate reduction by sulfate reducing bacteria (SRB) are the major hydrogenotrophic pathways in the human colon. Methanogenic status of mammals is suggested to be under evolutionary rather than dietary control. However, information is lacking regarding the dynamics of hydrogenotrophic microbial communities among different primate species. Methods Rectal swabs were collected from 10 sooty mangabeys (Cercocebus atys) and 10 baboons (Papio hamadryas). The diversity and abundance of methanogens and SRB were examined using PCR-denaturing gradient gel electrophoresis (DGGE) and real-time quantitative PCR (qPCR). Results The DGGE results revealed that intestinal Archaea and SRB communities differ between mangabeys and baboons. Phylogenetic analyses of Archaea DGGE bands revealed two distinct clusters with one representing a putative novel order of methanogenic Archaea. The qPCR detected a similar abundance of methanogens and SRB. Conclusions Intestinal Archaea and SRB coexist in these primates, and the community patterns are host species-specific.
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