期刊
JOURNAL OF MEDICAL MICROBIOLOGY
卷 62, 期 -, 页码 1060-1064出版社
MICROBIOLOGY SOC
DOI: 10.1099/jmm.0.049577-0
关键词
-
类别
资金
- Infectious Diseases Special Project [2008ZX10004-001B, 2009ZX10004-103, 2011ZX10004-001]
- Henan Medical Science Project [200702016]
- China-Australia Health and HIV/AIDS Facility [EID35]
A novel bunyavirus associated with fever, thrombocytopenia and leukopenia syndrome (FTLS) was discovered in Henan Province, China. Here, we report the development of an assay for this novel bunyavirus based on real-time reverse transcription PCR (RT-PCR). The assay exhibited high sensitivity and specificity without cross-reactivity towards 13 other viruses that cause similar symptoms. To evaluate the performance of this assay in detecting clinical samples, we analysed 261 serum samples from patients in Henan Province between 2007 and 2010. Of these samples, 91.95% were bunyavirus positive. Compared with serological assays, the real-time PCR assay was much more sensitive in identifying infected patients 1 to 7 days after the onset of symptoms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据