Article
Gastroenterology & Hepatology
Sa'ed H. Zyoud
Summary: This study provides the most up-to-date and comprehensive data on the trends in publications related to C.difficile infection. The number of published papers on this topic has significantly increased in the past decade. The United States is one of the countries with the highest publication rate. The study identifies three clusters of research areas, including illness spectrum and severity, laboratory diagnosis and characterization, and risk factors for C.difficile infection.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Review
Multidisciplinary Sciences
Tebelay Dilnessa, Alem Getaneh, Workagegnehu Hailu, Feleke Moges, Baye Gelaw
Summary: A high prevalence of C. difficile was observed among hospitalized patients, emphasizing the need for attention and prevention. The resistance of C. difficile to metronidazole and vancomycin was lower compared to other antibiotics.
Article
Immunology
Yuan-Pin Hung, Jen-Chieh Lee, Bo-Yang Tsai, Jia-Ling Wu, Hsiao-Chieh Liu, Hsiu-Chuan Liu, Hsiao-Ju Lin, Pei-Jane Tsai, Wen-Chien Ko
Summary: The risk factors of Clostridium difficile-associated diarrhea (CDAD) vary among hospitalized patients with different lengths of hospital stay. Malignancy, prior cephalosporin, and proton pump inhibitor therapy are independent risk factors for CDAD. Intervention strategies for preventing CDAD may need to be tailored based on the duration of hospital stay.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2021)
Article
Genetics & Heredity
Bilal Ahmed Abbasi, Aishwarya Dharan, Astha Mishra, Devansh Saraf, Irsad Ahamad, Prashanth Suravajhala, Jayaraman Valadi
Summary: In this study, the genomes of six strains of Clostridium difficile were annotated and characterized using in silico approaches. The functional properties of proteins involved in the pathophysiology of the disease were identified, providing valuable information for understanding the biological nature of the strains and developing pharmaceutical targets.
FRONTIERS IN GENETICS
(2022)
Article
Multidisciplinary Sciences
Amanda Nadia Diniz, Loren Nery Fontoura Moura, Diogo Soares Goncalves Cruz, Carlos Augusto Oliveira Junior, Henrique Cesar Pereira Figueiredo, Joao Luis Reis Cunha, Eduardo Garcia Vilela, Edward J. Kuijper, Mark H. Wilcox, Francisco Carlos Faria Lobato, Rodrigo Otavio Silveira Silva
Summary: Clostridioides difficile infection (CDI) is a global healthcare problem, and new strains continue to emerge. This study characterized three novel Brazilian clade 2 strains of C. difficile and conducted a two-year survey in animals and humans. The results suggest that these new clade 2 strains have virulence potential and continue to emerge in Brazil.
Article
Microbiology
Peng An Khun, Long Duc Phi, Phuong Thi Pham, Ha Thi Thu Nguyen, Quyen Thi Huyen Vu, Deirdre A. Collins, Thomas V. Riley
Summary: This study investigated the prevalence, molecular epidemiology, and antimicrobial susceptibility of Clostridioides difficile in children with diarrhea in Vietnam. The results showed a high prevalence of C. difficile, but a comparatively low proportion of toxigenic strains.
Article
Microbiology
Peng An Khun, Long Duc Phi, Huong Thi Thu Bui, Deirdre A. Collins, Thomas V. Riley
Summary: This study evaluated the epidemiology, molecular characteristics, and antimicrobial susceptibility of C. difficile isolated from adults with diarrhoea in Vietnam. The overall prevalence of C. difficile was 15.1%, with 9.8% being toxigenic strains and 6.3% being non-toxigenic strains. The prevalence of multidrug resistance was 27.3%. Rating: 8 out of 10.
Article
Chemistry, Multidisciplinary
Shuwen Qian, Dingran Chang, Jimmy Gu, Bruno J. Salena, Yingfu Li
Summary: Clostridium difficile is a common bacteria that causes an infectious disease, and there is a need for rapid diagnostic tests. Researchers have developed a RNA-cleaving fluorogenic DNAzyme probe, RFD-CD2, which can detect different pathogenic strains of C. difficile with high specificity. RFD-CD2 can detect C. difficile at a concentration as low as 100 CFU/mL, making it an attractive molecular probe for rapid diagnosis of CDI.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Virology
Andrzej Fitzner, Ewa Kwit, Wieslaw Niedbalski, Ewelina Bigoraj, Andrzej Kesy, Artur Rzezutka
Summary: The study analyzed the genetic and phylogenetic relationships of European brown hare syndrome virus (EBHSV) strains from Poland, finding high genetic homogeneity among the strains and their close relation to other EBHSV strains circulating in Europe. The slow evolutionary dynamics of this lagovirus species were confirmed, with variations in amino acid sequences identified in different genetic groups and VP10 sequences of nonpathogenic hare caliciviruses.
Article
Pathology
Sicilia Perumalsamy, Su Chen Lim, Thomas Riley
Summary: Less is known about Clostridioides difficile infection (CDI) in children compared to adults, and the rising incidence of CDI in hospitalized pediatric patients, emergence of hypervirulent strains and community-associated CDI (CA-CDI) in the past decade pose a potential threat. This study in a tertiary hospital in Perth, Western Australia, characterized C. difficile isolates from pediatric patients and found various ribotypes and toxin gene profiles. The study provides a baseline for future surveillance of pediatric CDI in Australia.
Article
Biology
Annamaria Toporova, Katarina Curova, Martin Novotny, Viera Lovayova, Maria Nagyova, Leonard Siegfried, Viktoria Takacova, Anna Liskova, Andrea Longauerova, Michaela Vukusiova Uhrinova, Barbora Dzugasova
Summary: In this study, we confirmed the presence of toxigenic and hypervirulent strains of Clostridioides difficile in stool samples of hospitalized patients. Ribotypes 176, 001, and 027 were identified as the most frequent, with ribotype 176 being predominant. The high incidence of rifampicin resistance in ribotypes 176 and 027 suggests the misuse of broad-spectrum antibiotics in our hospitals during the COVID-19 pandemic.
Article
Food Science & Technology
Parvin Ghorbani Filabadi, Ebrahim Rahimi, Amir Shakerian, Zahra Esfandiari
Summary: This study identified the prevalence and genetic diversities of Clostridium difficile contamination in meat nuggets in Isfahan, Iran. Out of 600 food samples, 7 C. difficile strains were found, with a prevalence of 1.17%. The isolated strains were found to be toxigenic and resistant to antibiotics, except for vancomycin. The absence of the tcdC gene in the genome of all isolates may be due to a mutation.
JOURNAL OF FOOD QUALITY
(2022)
Article
Infectious Diseases
Kacper Toczylowski, Katarzyna Jackowska, Dawid Lewandowski, Sandra Kurylonek, Marlena Waszkiewicz-Stojda, Artur Sulik
Summary: Rotavirus was the most common pathogen causing gastroenteritis in hospitalized patients aged 0-17 in northeastern Poland. The peak seasonality of rotavirus gastroenteritis occurred between February and May. Voluntary rotavirus vaccination did not impact the number of hospitalizations due to rotavirus AGE.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Biotechnology & Applied Microbiology
Nirajmohan Shivaperumal, Daniel R. Knight, Korakrit Imwattana, Grace O. Androga, Barbara J. Chang, Thomas Riley
Summary: This study reports the isolation of C.difficile strains from environmental samples in Western Australia that fail to produce black colonies and lack a putative beta-glucosidase gene. These strains also only produce toxin A. Four strains belong to a novel ribotype (QX 597) and show susceptibility to all tested antimicrobials.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Marta Dec, Dominik Lagowski, Tomasz Nowak, Dorota Pietras-Ozga, Klaudia Herman
Summary: The aim of this study was to characterize the phenotype and genotype of Erysipelothrix rhusiopathiae strains isolated from diseased pigs in Poland, and compare the SpaA sequence of wild-type strains with that of the R32E11 vaccine strain. Antibiotic susceptibility, resistance genes, virulence genes, and serotype determinants were investigated. The study found that the E. rhusiopathiae strains in Poland are diverse in terms of serotype and SpaA variant, and differ from the vaccine strain antigenically. Beta-lactams, macrolides, or phenicols are recommended for the treatment of swine erysipelas in Poland, but caution should be exercised due to the small number of strains tested.
Article
Microbiology
Pawel Karpinski, Dorota Wultanska, Michal Piotrowski, Marie Brajerova, Agnieszka Mikucka, Hanna Pituch, Marcela Krutova
Summary: The motility and genotype of flagellin fliC and fliD genes were investigated in 82 C. difficile isolates, with RT023 showing the highest motility similar to hypervirulent RT027 and its genetic relative RT176.
Article
Microbiology
Michal Piotrowski, Dorota Wultanska, Hanna Pituch
Summary: The study aimed to determine the effect of standard and candidate prebiotics on the adhesion and biofilm formation of Bacteroides sp. The results showed that prebiotics and synbiotics may help decrease the burden of C. difficile infections.
FUTURE MICROBIOLOGY
(2022)
Article
Immunology
Michal Piotrowski, Dorota Wultanska, Hanna Pituch
Summary: This study investigated the effects of 3'-sialyllactose (SL) and 6'-SL on adhesion and biofilm formation of C. difficile. The results showed that both substances can inhibit the adhesion of C. difficile to human colon cells and reduce biofilm formation.
MICROBES AND INFECTION
(2022)
Article
Infectious Diseases
Virginie F. Viprey, Georgina L. Davis, Anthony D. Benson, Duncan Ewin, William Spittal, Jon J. Vernon, Maja Rupnik, Alice Banz, Florence Allantaz, Philippe Cleuziat, Mark H. Wilcox, Kerrie A. Davies
Summary: This study compared data on CDI patients in hospitals and communities across 12 European countries. The study found a higher CDI positivity rate in hospitals and a larger number of undiagnosed adults in the community, highlighting the need for improved diagnosis of diarrhea patients in the community.
Article
Biochemistry & Molecular Biology
Dorota Wultanska, Bohdan Paterczyk, Julita Nowakowska, Hanna Pituch
Summary: The study investigated the antibacterial and antibiotic activity of bee products against Clostridioides difficile strains with different ribotypes. The research found that Manuka honey, pine honey, and bee bread showed antibacterial activity against C. difficile strains, and at subinhibitory concentrations, they increased the adhesion of the bacteria.
Article
Pediatrics
Marcin Dziekiewicz, Honorata Marczak, Marcin Banasiuk, Marina Aksionchyk, Katarzyna Krenke, Aleksandra Banaszkiewicz
Summary: This study found a relatively high frequency of GERD in children with chILD, but no association between proximal GER and GERD diagnosis, which casts uncertainty over the microaspiration theory proposed to link the two diseases. However, more accurate aspiration assessment methods are needed to confirm this conclusion.
PEDIATRIC PULMONOLOGY
(2023)
Article
Gastroenterology & Hepatology
Aleksandra Banaszkiewicz, Jan Bukowski, Jan Pertkiewicz, Lukasz Dembinski, Ewa Kluczewska, Jaroslaw Kwiecien
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
(2023)
Editorial Material
Gastroenterology & Hepatology
Lukasz Dembinski, Marcin Dziekiewicz, Marcin Banasiuk, Aleksandra Banaszkiewicz
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
(2023)
Article
Immunology
Marta Kierzkowska, Anna Majewska, Konrad Karlowicz, Hanna Pituch
Summary: The aim of this study was to determine the prevalence of cfiA-positive isolates in B. fragilis infections and investigate the carbapenemase activity in B. fragilis strains through the Carba NP test. The study found that 5.2% of B. fragilis isolates were phenotypically resistant to meropenem and 6.1% of B. fragilis isolates carried the cfiA gene. The meropenem minimum inhibitory concentrations were significantly higher in cfiA-positive strains.
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2023)
Article
Medicine, General & Internal
Urszula Daniluk, Paulina Krawiec, Elzbieta Pac-Kozuchowska, Lukasz Dembinski, Jan Stanislaw Bukowski, Aleksandra Banaszkiewicz, Anna Wozniuk-Kazmierczak, Elzbieta Czkwianianc, Jan Brylak, Jaroslaw Walkowiak, Agnieszka Borys-Iwanicka, Anna Kofla-Dlubacz, Tomasz Pytrus, Katarzyna Zdanowicz, Dariusz Marek Lebensztejn
Summary: The coexistence of inflammatory bowel disease (IBD) with pancreatic pathology is rare in children. A retrospective analysis of data from 1538 children diagnosed with IBD in 2014-2021 found that 11.4% of them had pancreatic involvement (PI), with acute pancreatitis (AP) diagnosed in 43.8% and asymptomatic hyperlipasemia (HL) or hyperamylasemia (HA) observed in 50.0%. Routine monitoring of pancreatic enzymes should be performed in patients with IBD, especially after the initiation of azathioprine (AZA) treatment.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Microbiology
Ishani Wickramage, Zhong Peng, Soumyadeep Chakraborty, Celine Harmanus, Ed J. Kuijper, Sally Alrabaa, Wiep Klaas Smits, Xingmin Sun
Summary: Clostridioides difficile has a complex relationship with antibiotics, as they can both increase the risk of infection and be the primary treatment for C. difficile infection (CDI). This study identified two clinical isolates of C. difficile with elevated resistance to vancomycin and rifaximin, as well as mutations related to resistance. The findings highlight the importance of surveillance in understanding antibiotic resistance and developing effective treatment strategies.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Dorota Wultanska, Pawel Karpinski, Michal Piotrowski, Hanna Pituch
Summary: Sub-MIC concentrations of metronidazole affect the biofilm formation of clinical and reference strains of Clostridioides difficile, particularly inducing biofilm formation in hypervirulent RT027 strains.
Article
Microbiology
P. M. C. Klein Klouwenberg, W. van der Kuil, A. J. van Griethuysen, M. Hendriks, E. J. Kuijper, D. W. Notermans, A. F. Schoffelen, ISIS AR Study Grp
Summary: Using data from the Dutch national surveillance system for antimicrobial resistance, this study found discrepancies in minimum inhibitory concentrations of aminoglycosides measured by the automated testing systems VITEK2 and Phoenix. The Phoenix system showed higher MICs and an annual increase in resistance for certain species. These findings have implications for the clinical treatment of sepsis patients.
MICROBIOLOGY SPECTRUM
(2023)