Article
Critical Care Medicine
Jaipal Singh, Young Lee, John A. Kellum
Summary: The nitric oxide pathway is important in vascular homeostasis, and its role in sepsis and shock is still not fully understood. The endogenously produced inhibitor of nitric oxide synthase, asymmetric dimethylarginine, may play a significant role in the pathophysiology of sepsis and ischemia-reperfusion.
Article
Nutrition & Dietetics
Damian Gajecki, Jakub Gawrys, Jerzy Wisniewski, Paulina Fortuna, Ewa Szahidewicz-Krupska, Adrian Doroszko
Summary: The study reveals significant differences in the nitric oxide metabolic pathway in patients with type 2 diabetes, particularly in the plasma compartment. Erythrocytes serve as a buffer with higher nitric oxide bioavailability, less affected by diabetes-related deviations. Individuals with type 2 diabetes show endothelial dysfunction.
Review
Biochemistry & Molecular Biology
Jakub Mochol, Jakub Gawrys, Damian Gajecki, Ewa Szahidewicz-Krupska, Helena Martynowicz, Adrian Doroszko
Summary: Obstructive sleep apnea is considered an independent cardiovascular risk factor, and intermittent hypoxia is now recognized as a key pathophysiological mechanism contributing to endothelial dysfunction. This review focuses on molecular mechanisms triggered by sleep apnea, particularly the effects of treatment with continuous positive airway pressure on endothelial function and potential clinical advantages. Additionally, the paper explores how OSA may affect platelet function and rheological activity of erythrocytes, potentially increasing the risk of stroke.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Vinitha N. Ragavan, Pramod C. Nair, Natalia Jarzebska, Ramcharan Singh Angom, Luana Ruta, Elisa Bianconi, Silvia Grottelli, Natalia D. Tararova, Daniel Ryazanskiy, Steven R. Lentz, Sara Tommasi, Jens Martens-Lobenhoffer, Toshiko Suzuki-Yamamoto, Masumi Kimoto, Elena Rubets, Sarah Chau, Yingjie Chen, Xinli Hu, Nadine Bernhardt, Peter M. Spieth, Norbert Weiss, Stefan R. Bornstein, Debabrata Mukhopadhyay, Stefanie M. Bode-Boeger, Renke Maas, Ying Wang, Antonio Macchiarulo, Arduino A. Mangoni, Barbara Cellini, Roman N. Rodionov
Summary: An international consortium of research groups used various methods to investigate whether DDAH2 directly metabolizes ADMA, the risk factor for cardiovascular disease. Their findings unanimously showed that DDAH2 is unable to metabolize ADMA, resolving a 20-year controversy and providing a starting point for exploring alternative functions of DDAH2.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Annapoorna Mohandas, Jayakumar Rangasamy
Summary: This study developed an injectable nanocurcumin and arginine incorporated chitosan hydrogel to prevent hypoxia induced endothelial damage. The hydrogel showed significant antioxidant activity and biodegradation in vitro, with better release of curcumin and arginine at acidic pH. The hydrogel effectively reduced the effect of hypoxia induced endothelial dysfunction in cultured endothelial cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Cardiac & Cardiovascular Systems
Karsten Piatek, Anna Feuerstein, Veronika Zach, Cristina Rozados da Conceicao, Amelie Beblo, Evgeny Belyavskiy, Elisabeth Pieske-Kraigher, Alexander Krannich, Edzard Schwedhelm, Sarah Hinz, Burkert Pieske, Frank Edelmann
Summary: The impaired NO metabolism is suggested as a possible key pathogenic determinant in HFpEF patients. Further evaluation and therapeutic interventions targeting NO metabolism and pathway should be considered.
Article
Pharmacology & Pharmacy
Zheyi Yan, Xiaoming Cao, Chunfang Wang, Sha Liu, Yanjie Li, Gan Lu, Wenjun Yan, Rui Guo, Dajun Zhao, Jimin Cao, Yong Xu
Summary: CTRP3 improves vasodilation of microvasculature in diabetes by enhancing endothelial cell function through the AMPK/eNOS/NO. signaling pathway.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maciej Bladowski, Ewa Szahidewicz-Krupska, Jerzy Wisniewski, Paulina Fortuna, Justyna Chojdak-Lukasiewicz, Slawomir Budrewicz, Mariusz Fleszar, Adrian Doroszko
Summary: This study aimed to analyze the differences in the expression of the nitric oxide metabolic pathway in platelets and plasma between ischemic stroke patients and non-stroke individuals. It also investigated the relationship between these parameters and platelet aggregation. The results showed that stroke patients had higher levels of NOS inhibitors in platelets, and stroke management increased the bioavailability of nitric oxide in platelets.
Article
Biochemistry & Molecular Biology
S. Contreras-Duarte, C. Cantin, M. Farias, A. Leiva
Summary: This study evaluated the effects of MSPD on GDM and how these pathologies together affect fetoplacental endothelial function. Results showed increased TC and TG in MSPD and GDM-MSPD groups, decreased eNOS activity, increased hCat1 protein and L-arginine transport, and higher Arginase II protein and activity in GDM-MSPD compared to MPD.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Ophthalmology
A. Brantley Milam, Katherine S. Peters, Emilio Rivera, Cassandra Warden, Paula A. Harlow, Sabrina L. Mitchell, M. Wade Calcutt, David C. Samuels
Summary: This study aimed to investigate the differences in six arginine-related and citrulline-related metabolites between patients with type 2 diabetes and diabetic retinopathy. The results showed that plasma levels of arginine and citrulline were significantly elevated in patients with diabetic retinopathy.
AMERICAN JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Medicine, General & Internal
Jakub Jasiczek, Malgorzata Trocha, Arkadiusz Derkacz, Ewa Szahidewicz-Krupska, Adrian Doroszko
Summary: The study did not confirm the modulatory effect of valsartan on endothelial function. ASA treatment minimized differences between hypertensive and normotensive groups, and increased PRA in the N-subgroup. In hypertensive subjects, inflammatory markers were higher while angiogenic factors did not differ significantly between groups.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Anna Drohomirecka, Joanna Was, Natalia Wiligorska, Tomasz M. Rywik, Krzysztof Komuda, Dorota Sokolowska, Anna Lutynska, Tomasz Zielinski
Summary: Methylated arginine metabolites can disrupt nitric oxide synthesis, leading to endothelium dysfunction and inadequate vasodilation. This study aimed to investigate the dynamics of arginine derivatives during physical exercise in heart failure patients and assess its impact on patient outcomes.
Article
Multidisciplinary Sciences
Ibrahim Musa Yola, Carlee Moser, Meredith S. Duncan, Edzard Schwedhelm, Dorothee Atzler, Renke Maas, Juliane Hannemann, Rainer H. Boeger, Ramachandran S. Vasan, Vanessa Xanthakis
Summary: The study found a significant association between the endogenous nitric oxide synthase inhibitor ADMA and the prevalence of MetS, but no significant associations were found between other methylarginines and the incidence of MetS. Further studies with larger sample sizes are needed to replicate these findings.
Article
Medicine, Research & Experimental
Shupeng Hu, Qiangzhong Pi, Xiudan Xu, Jianghong Yan, Yongzheng Guo, Wanying Tan, An He, Zhe Cheng, Suxin Luo, Yong Xia
Summary: This study investigates the role of eNOS in vascular dysfunction induced by sepsis. Increased phosphorylation of eNOS in early sepsis leads to decreased vasodilation, while decreased levels of eNOS in late sepsis result in diminished nitric oxide-dependent relaxation responses.
Article
Medicine, Research & Experimental
Jun -Ha Hwang, Woong Heo, Jung Il Park, Kyung Min Kim, Ho Taek Oh, Gi Don Yoo, Jeekeon Park, Somin Shin, Youjin Do, Mi Gyeong Jeong, Eun Sook Hwang, Jeong-Ho Hong
Summary: Endothelial TAZ is involved in the production of nitric oxide (NO) and inhibits damage-induced liver fibrosis. It regulates NO production by transcriptional regulation of Nos3. Loss of TAZ leads to capillarization of liver sinusoidal endothelial cells and a decrease in NO concentration, accelerating liver fibrosis.
Article
Pharmacology & Pharmacy
Radmila Velickovic-Radovanovic, Nikola Stefanovic, Ivana Damnjanovic, Branislava Kocic, Snezana Mladenovic-Antic, Marina Dinic, Jasmina Petrovic, Radmila Mitic, Aleksandra Catic-Dordevic
Summary: The study found that cephalosporins were the most commonly used antibiotics from 2014 to 2018, with an increasing trend in susceptibility of MDR Escherichia coli isolates to ceftriaxone, but a decreasing trend in susceptibility to ciprofloxacin and levofloxacin. Klebsiella spp MDR isolates showed a decreasing trend in susceptibility to cefepime and both fluoroquinolones, with a significant negative association between cefepime consumption and Klebsiella spp MDR isolates susceptibility.
EUROPEAN JOURNAL OF HOSPITAL PHARMACY
(2022)
Article
Microbiology
Mila Bojanovic, Aleksandra Ignjatovic, Marko Stalevic, Valentina Arsic-Arsenijevic, Marina Randelovic, Vladimir Gerginic, Zorica Stojanovic-Radic, Ognjen Stojkovic, Emilija Zivkovic-Marinkov, Suzana Otasevic
Summary: This study investigated the clinical presentation, diagnostic procedure, and relapse in patients with Aspergillus-otomycosis, as well as the biofilm production ability of relapse isolates. The results showed high sensitivity of microscopy and the presence of a specific patient cluster with A. niger complex-otomycosis. Six patients experienced relapse with the same Aspergillus species, all of which had the ability to produce biofilm. Official criteria and recommendations are needed to prevent misdiagnosis and complications.
Review
Cardiac & Cardiovascular Systems
Dijana Stojanovic, Valentina Mitic, Miodrag Stojanovic, Jelena Milenkovic, Aleksandra Ignjatovic, Maja Milojkovic
Summary: Cardiac fibrosis is characterized by excessive accumulation of extracellular matrix proteins, leading to ineffective healing and ultimately heart failure. Renalase, a novel protein secreted by the kidneys, shows promise as an anti-fibrotic factor by reducing inflammation, oxidative stress, and fibrotic responses through various mechanisms.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Review
Cell Biology
Dijana Stojanovic, Miodrag Stojanovic, Jelena Milenkovic, Aleksandra Velickov, Aleksandra Ignjatovic, Maja Milojkovic
Summary: The hallmark of COVID-19 is an inappropriate immune response that leads to inflammation and oxidative stress, resulting in acute respiratory distress syndrome and fibrosis. Renalase, a novel amino-oxidase derived from the kidneys, has shown significant organ protection and potential to alleviate inflammation and fibrosis. Using renalase in experimental models of COVID-19 could be a valuable approach to target acute phase mechanisms and halt fibrosis progression.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Chemistry, Physical
Goran M. Nikolic, Slavoljub C. Zivanovic, Milica G. Nikolic, Aleksandar M. Veselinovic
Summary: Numerous studies have shown that caffeic acid has various potential uses in medications and cosmetics, as well as therapeutic implications in cardiovascular diseases, various types of cancer, and neurological diseases. This study investigated the kinetics and reaction mechanism of caffeic acid autoxidation in weakly alkaline aqueous solutions in the presence of Mg(II) ions. The results revealed that the presence of Mg(II) ions significantly increased the autoxidation rate and led to the formation of quinones and polymeric products.
REACTION KINETICS MECHANISMS AND CATALYSIS
(2023)
Article
Food Science & Technology
Susanna Draghi, Radmila Pavlovic, Alberto Pellegrini, Marco Fidani, Federica Riva, Gabriele Brecchia, Stella Agradi, Francesco Arioli, Daniele Vigo, Federica Di Cesare, Giulio Curone
Summary: This study investigated the distribution of 14 different per-fluoroalkyl substances (PFASs) within milk by analyzing the content in whole milk, skim milk, and cream. Samples were taken from 23 cows in Northern Italy that were not exposed to known sources of PFASs. The most common PFAS found in all fractions was perfluorobutanoic acid (PFBA), followed by perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA). PFOS concentration was higher in cream compared to raw and skimmed milk. Multiparous cows had higher levels of PFASs in their milk. Further research is needed to assess the risks of consuming dairy products contaminated with PFASs and their impact on cattle health.
Review
Microbiology
Mila Bojanovic, Marko Stalevic, Valentina Arsic-Arsenijevic, Aleksandra Ignjatovic, Marina Randelovic, Milan Golubovic, Emilija Zivkovic-Marinkov, Goran Koracevic, Bojana Stamenkovic, Suzana Otasevic
Summary: Otomycosis is a common fungal infection of the external auditory canal worldwide, with prevalence ranging from 9% to 30%. Aspergillus niger complex and Candida spp. are the main causative agents, with a wide range of other fungi also responsible. The prevalence of otomycosis is highest in Iran, India, China, Egypt, Mexico, and Brazil. Diagnosis is challenging as it can be difficult to distinguish from other infections, but laboratory-based evidence is essential. Treatment involves the use of various topical and systemic antifungal agents.
Review
Cardiac & Cardiovascular Systems
Vladica M. Velickovic, Daniel Carradice, Jonathan R. Boyle, Mohamad Hamady, Trevor Cleveland, Simon Neequaye, Aleksandra Ignjatovic, Dragana Bogdanovic, Jelena Savovic, Uwe Siebert
Summary: This umbrella review aimed to assess the quality of published meta-analyses, conduct a de-novo meta-analysis of available RCTs, and test the hypothesis of long-term mortality difference between OSR and EVAR. The results showed that EVAR had significantly favorable mortality up to four years, but there was no difference between EVAR and OSR in the longest available time period. The Cox model demonstrated a non-significant hazard ratio in favor of OSR, while the best-fitting generalized gamma model showed a slightly significant hazard ratio in favor of EVAR.
EXPERT REVIEW OF CARDIOVASCULAR THERAPY
(2023)
Article
Education, Scientific Disciplines
Aleksandra Ignjatovic, Lazar Stevanovic
Summary: The study assessed ChatGPT's (GPT-3.5 and GPT-4) performance as a study tool in solving biostatistical problems and found that both versions had below-average performance. While GPT-3.5 and GPT-4 were able to provide correct answers for many problems, they struggled with certain aspects, particularly ANOVA, chi-square test, and sample size-related questions. Therefore, students need to be aware of the limitations and exercise caution when incorporating this model into medical education.
JOURNAL OF EDUCATIONAL EVALUATION FOR HEALTH PROFESSIONS
(2023)
Article
Pharmacology & Pharmacy
Marija Jovanovic, Milena Kovacevic, Aleksandra Catic-Dordevic, Milica Culafic, Nikola Stefanovic, Branka Mitic, Katarina Vucicevic, Sandra Vezmar Kovacevic, Radmila Velickovic-Radovanovic, Branislava Miljkovic
Summary: This study compared the prevalence and types of potentially inappropriate medications (PIMs) and potential prescribing omissions (PPOs) between the original and updated versions of STOPP/START criteria among older patients in different settings. The prevalence of STOPP v2 was slightly higher, but not statistically significant, compared to v1, while the prevalence of START v2 was significantly lower than v1. The most commonly identified PIMs in STOPP v2 were drug indication and fall-risk medications, while cardiovascular system criteria were the most frequently detected in START v2. The number of medications was a strong predictor for both STOPP v1 and v2. The study highlights a substantial rate of potentially inappropriate prescribing among elderly patients.
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Ivana Damnjanovic, Nastia Tsyplakova, Nikola Stefanovic, Tatjana Tosic, Aleksandra Catic-Dordevic, Vangelis Karalis
Summary: This study used nonlinear mixed-effect modeling to describe the pharmacokinetics of valproic acid (VA), lamotrigine (LTG), and levetiracetam (LEV) in a pediatric population. Machine learning algorithms were applied to identify relationships between plasma levels of the medications and patient characteristics, as well as develop a predictive model for epileptic seizures. The results showed that the kinetics of LTG, LEV, and VA can be best described by a one compartment model with first-order absorption and elimination kinetics. The main factors affecting antiepileptic activity are drug levels and body weight, while gender has no influence. The application of pharmacokinetic and machine learning models can improve epilepsy management during the growth and development of children.
THERAPEUTIC ADVANCES IN DRUG SAFETY
(2023)
Article
Allergy
Vesna Karanikolic, Aleksandra Ignjatovic, Mirjana Marinkovic, Lidija Djordjevic
Summary: This study compared the effectiveness of two different sub-bandage pressure values in treating venous leg ulcers. The results showed that applying a higher pressure led to faster healing and improved quality of life for the patients.
POSTEPY DERMATOLOGII I ALERGOLOGII
(2023)
Review
Medicine, General & Internal
Vladana Stojiljkovic, Nikola Stefanovic, Marija Vukelic Nikolic, Branka Djordjevic, Jelena Basic, Gordana Kocic, Tatjana Cvetkovic
Summary: MicroRNAs have been suggested as potential biomarkers for the diagnosis of chronic kidney disease, especially in oncology. However, there are challenges to be addressed in order to use microRNAs routinely.
ACTA FACULTATIS MEDICAE NAISSENSIS
(2023)
Meeting Abstract
Medical Laboratory Technology
V. Stojiljkovic, T. Cvetkovic, V. Cosic, S. Stojiljkovic, L. Zvezdanovic, M. Cvetkovic, N. Stefanovic
CLINICA CHIMICA ACTA
(2022)
Meeting Abstract
Medical Laboratory Technology
T. Cvetkovic, N. Stevic, N. Stefanovic, V. Cosic, M. Cvetkovic, V. Stoiljkovic, Z. Dimitrijevic, B. Mitic
CLINICA CHIMICA ACTA
(2022)