Article
Biochemistry & Molecular Biology
Sergio Cortez Ghio, Martin A. Barbier, Emilie J. Doucet, Imad Debbah, Meryem Safoine, Gaetan Le-Bel, Andreanne Cartier, Emilie Jolibois, Amelie Morissette, Danielle Larouche, Julie Fradette, Sylvain L. Guerin, Alain Garnier, Lucie Germain
Summary: We developed a new chemically defined serum-free medium, Surge SFM, which can support massive expansion of human primary keratinocytes and production of skin substitutes. Compared to conventional serum-containing medium, Surge SFM can more efficiently expand keratinocytes and properly form stratified epidermis. It also retains keratin-19-positive epithelial stem cells and maintains similar gene expression as with the conventional medium. Surge SFM can be used to produce bilayered self-assembled skin substitutes histologically similar to those produced using serum-containing medium, which can persist for up to six months when grafted onto mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Aliosha I. Figueroa-Valdes, Catalina de la Fuente, Yessia Hidalgo, Ana Maria Vega-Letter, Rafael Tapia-Limonchi, Maroun Khoury, Francisca Alcayaga-Miranda
Summary: Cell therapy is shifting towards cell-free treatments using small extracellular vesicles (sEV) that mimic the effects of parental cells, but faces challenges in large-scale production and regulation compliance. Using a regulatory-compliant medium like Oxium(TM)EXO can significantly increase sEV secretion rates and improve production efficiency.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Yun Chang, Ramizah Syahirah, Stephanie N. Oprescu, Xuepeng Wang, Juhyung Jung, Scott H. Cooper, Sandra Torregrosa-Allen, Bennett D. Elzey, Alan Y. Hsu, Lauren N. Randolph, Yufei Sun, Shihuan Kuang, Hal E. Broxmeyer, Qing Deng, Xiaojun Lian, Xiaoping Bao
Summary: This study demonstrates the successful generation of AGM-like hematopoietic system from human pluripotent stem cells through temporal manipulation of Wnt signaling. The generated hematopoietic progenitor cells resemble primary cord blood HSCs at the transcriptional level and exhibit lymphoid and myeloid potential in vitro. The cells also show the ability to home to a definitive hematopoietic site, rescue bloodless zebrafish, and engraft and repopulate in mouse recipients.
Review
Anatomy & Morphology
A. Thirumal Raj, Supriya Kheur, Ramesh Bhonde, Archana A. Gupta, Shankargouda Patil
Summary: This study assessed the effect of human MSC derived conditioned media on human cancer cell lines, identifying pro-carcinogenic and anti-carcinogenic molecules and effector pathways associated with different MSC types. The use of MSC-CM with pro-carcinogenic effect should be restricted in cancer patients regardless of the application nature.
Article
Cell Biology
Stefano Panella, Francesco Muoio, Valentin Jossen, Yves Harder, Regine Eibl-Schindler, Tiziano Tallone
Summary: Adipose tissue is a rich source of stem cells, but liposuction cannot provide enough for direct applications in regenerative medicine. Hence, GMP-compliant ex vivo expansion protocols are needed to ensure safe, reproducible, and cost-effective production of cell drugs. The developed UrSuppe cell culture medium allows for the cultivation and differentiation of human adipose stem cells, potentially facilitating the translation of research projects into clinical applications.
Article
Engineering, Biomedical
Wanjun Liang, Yan Li, Jose Luis Cuellar-Camacho, Leixiao Yu, Suqiong Zhou, Wenzhong Li, Rainer Haag
Summary: The development of induced pluripotent stem cells (iPSCs) has provided significant opportunities for biomedical applications. However, improving cell-culturing approaches for reliable expansion of iPSCs remains a challenge. This study introduces a chemically defined, artificial three-dimensional microniche that allows for robust production of iPSCs without pathogen or immunogen transfer risks.
Article
Biochemistry & Molecular Biology
Jeonghyun Kim, Takashi Inagaki, Junko Sunaga, Taiji Adachi, Takeo Matsumoto
Summary: This study elucidated the multicellular behaviors of osteocytic spheroids in response to osteogenesis supplements. The results showed that the supplements altered the spheroids' behaviors by affecting their size, fusion speed, and collagen embedding. Actin filaments were identified as the driving force behind these changes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Nanoscience & Nanotechnology
Jun Yong Kim, Won-Kyu Rhim, Seung-Gyu Cha, Jiwon Woo, Joo Youn Lee, Chun Gwon Park, Dong Keun Han
Summary: The combination of three-dimensional culture and cell priming provides the optimal environment for maximizing secretion and regeneration-related bioactivities of mesenchymal stem cell-derived extracellular vesicles without impurities.
Article
Polymer Science
Samuel R. R. Moxon, David Richards, Oana Dobre, Lu Shin Wong, Joe Swift, Stephen M. M. Richardson
Summary: Cell function can be influenced by the mechanical and structural properties of the extracellular environment. This study presents a system of polyacrylamide hydrogels that can be tailored in surface topography and matrix stiffness in situ with photoirradiation. The system offers a new platform to study cellular mechanosensing and the influence of extracellular cues on cell phenotype and function.
Article
Multidisciplinary Sciences
Masatoshi Sakurai, Kantaro Ishitsuka, Ryoji Ito, Adam C. Wilkinson, Takaharu Kimura, Eiji Mizutani, Hidekazu Nishikii, Kazuhiro Sudo, Hans Jiro Becker, Hiroshi Takemoto, Tsubasa Sano, Keisuke Kataoka, Satoshi Takahashi, Yukio Nakamura, David G. Kent, Atsushi Iwama, Shigeru Chiba, Shinichiro Okamoto, Hiromitsu Nakauchi, Satoshi Yamazaki
Summary: Haematopoietic stem cells (HSCs) can reconstitute the entire blood and immune systems after transplantation and have the potential to be used as a curative cell therapy for various haematological diseases. However, the low number of HSCs and the limited ex vivo expansion ability of human HSCs pose challenges for biological analysis and clinical application. In this study, a culture system was established using chemical agonists and a caprolactam-based polymer, which successfully promoted long-term ex vivo expansion of human HSCs. The chemically defined expansion culture system will contribute to the advancement of clinical HSC therapies.
Article
Cell & Tissue Engineering
Marwan M. Merkhan, Matthew T. Shephard, Nicholas R. Forsyth
Summary: Modulation of environmental oxygen alters both the concentration and composition of the human mesenchymal stem cell (hMSC) secretome, which is likely to have significant implications for improving understanding and efficacy of hMSC-based therapeutics.
JOURNAL OF TISSUE ENGINEERING
(2021)
Article
Multidisciplinary Sciences
Eiko Shimizu, Hiroki Iguchi, Minh Nguyen Tuyet Le, Yuta Nakamura, Daigo Kobayashi, Yuhei Arai, Kenta Takakura, Seiko Benno, Noriko Yoshida, Masayoshi Tsukahara, Satoshi Haneda, Kouichi Hasegawa
Summary: In this study, a chemically synthetic culture substrate was developed for the expansion and differentiation of human pluripotent stem cells. The substrate exhibited thermotolerance and underwater stability, and could be stored at room temperature. It supported hPSC expansion with similar efficiency to commercial substrates and enabled directed differentiation.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Nela Pilbauerova, Jan Schmidt, Tereza Suchankova Kleplova, Tomas Soukup, Jakub Suchanek
Summary: Despite some scientific or ethical concerns, fetal bovine serum (FBS) remains the standard nutrient supplement for cultivating mesenchymal stem cells. However, recent research has found that human platelet lysate (hPL) is a suitable alternative. This study evaluated the effects of 2% hPL on the in vitro expansion of human natal dental pulp stem cells (hNDP-SCs), and found that hPL can significantly enhance cell proliferation.
Article
Biochemistry & Molecular Biology
Victoria Karakis, Mahe Jabeen, John W. Britt, Abigail Cordiner, Adam Mischler, Feng Li, Adriana San Miguel, Balaji M. Rao
Summary: Human trophoblast stem cells (hTSCs) can be differentiated into extravillous trophoblast (EVT) or multinucleate syncytiotrophoblast (STB) lineages in a chemically defined culture system. The presence of laminin-111 can switch the terminal differentiation from STB to EVT. Furthermore, the inhibition of TGFP signaling affects both STB and EVT differentiation mediated by laminin-111 exposure.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Polymer Science
Lorenzo Bonetti, Luigi De Nardo, Silvia Fare
Summary: The study successfully prepared thermoresponsive methylcellulose (MC) hydrogels using a crosslinking strategy based on citric acid (CA) and demonstrated their potential as substrates in cell sheet engineering. The hydrogels showed improved mechanical performance and cell adhesion, allowing for easy detachment of cell sheets for regenerative purposes.
Article
Materials Science, Biomaterials
John D. Krutty, Kevin Koesser, Stephen Schwartz, Junsu Yun, William L. Murphy, Padma Gopalan
Summary: This study created chemically defined three-dimensional surfaces for large-scale cell expansion by applying a synthetic polymer coating on commercially available polystyrene microcarriers, providing a reproducible surface without relying on xenogeneic serum proteins. These microcarriers not only improved the adhesion of hMSC in serum-free media but also allowed efficient cell passage without the use of cleavage enzymes.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Huihua Yuan, Xiaolei Li, Ming-Song Lee, Zhuojun Zhang, Biyun Li, Hongyun Xuan, Wan-Ju Li, Yanzhong Zhang
Summary: The study developed highly-aligned poly(L-lactic acid) fibers decorated with type 1 collagen and chondroitin sulfate, which showed enhanced tenogenic differentiation of human mesenchymal stem cells in vitro. The biomimetic fibers promoted cell spreading and proliferation, increased expression of tendon-associated genes and proteins, and activated the TGF-beta signaling pathway. Animal study validated the promoting role of these fibers in tendon regeneration.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biophysics
Matthew M. Grondin, Fang Liu, Michael F. Vignos, Alexey Samsonov, Wan-Ju Li, Richard Kijowski, Corinne R. Henak
Summary: This study demonstrated that fast relaxing macromolecular bound water component fraction (FF) measured using mcDESPOT at 3.0 T is significantly correlated with energy dissipation, linear modulus, and proteoglycan content in cartilage. Other mcDESPOT parameters showed limited correlation with mechanical and chemical properties of cartilage.
JOURNAL OF BIOMECHANICS
(2021)
Article
Cell & Tissue Engineering
Kannan Manian, Chad A. Galloway, Sonal Dalvi, Anthony A. Emanuel, Jared A. Mereness, Whitney Black, Lauren Winschel, Celia Soto, Yiming Li, Yuanhui Song, William DeMaria, Akhilesh Kumar, Igor Slukvin, Michael P. Schwartz, William L. Murphy, Bela Anand-Apte, Mina Chung, Danielle S. W. Benoit, Ruchira Singh
Summary: Researchers have developed a 3D RPE-CC model that mimics key features of healthy and AMD/MD eyes while providing modular control over RPE and CC layers. They have found that both RPE and mesenchyme-secreted factors are necessary for the formation of fenestrated CC-like vasculature, and that CC atrophy and/or CNV can be initiated by patient serum or mutant RPE-secreted factors. Additionally, FGF2 and matrix metalloproteinases have been identified as potential therapeutic targets for AMD/MDs.
Correction
Cell & Tissue Engineering
Kannan V. Manian, Chad A. Galloway, Sonal Dalvi, Anthony A. Emanuel, Jared A. Mereness, Whitney Black, Lauren Winschel, Celia Soto, Yiming Li, Yuanhui Song, William DeMaria, Akhilesh Kumar, Igor Slukvin, Michael P. Schwartz, William L. Murphy, Bela Anand-Apte, Mina Chung, Danielle S. W. Benoit, Ruchira Singh
Article
Cell & Tissue Engineering
Angela W. Xie, Nicholas A. Zacharias, Bernard Y. K. Binder, William L. Murphy
Summary: This study compared MSC aggregates generated via three different methods and found differences in their structure and immunomodulatory phenotype under resting conditions and in response to inflammatory stimulus. The methods with faster aggregation kinetics formed aggregates with higher cell packing density and reduced ECM synthesis.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
M. Orth, T. Fritz, J. Stutz, C. Scheuer, B. Ganse, Y. Bullinger, J. S. Lee, W. L. Murphy, M. W. Laschke, M. D. Menger, T. Pohlemann
Summary: MCM + VB, loaded with VEGF and BMP-2, improves bone healing in atrophic non-unions by promoting angiogenesis and osteogenesis, as well as reducing early osteoclast activity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Andrew S. Khalil, Daniel Hellenbrand, Kaitlyn Reichl, Jennifer Umhoefer, Mallory Filipp, Joshua Choe, Amgad Hanna, William L. Murphy
Summary: Spinal cord injuries often have devastating consequences and limited therapeutic options. This study utilizes mineral-coated microparticles as efficient non-viral gene delivery vehicles to produce exogenous ChABC in spinal cord injuries, reducing CSPG deposition and improving motor function recovery.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Cell & Tissue Engineering
Anna Laura Nelson, GianLuca Fontana, Elizabeth Miclau, Mallory Rongstad, William Murphy, Johnny Huard, Nicole Ehrhart, Chelsea Bahney
Summary: This article reviews methods to enhance bone formation and improve bone repair, focusing on the challenges and solutions for activating the Wnt pathway through antibody-based therapeutics, synthetic Wnt surrogates, and scaffold doping. Additionally, emerging technologies such as mRNA therapy and CRISPR technology for clinical translation are discussed.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Multidisciplinary Sciences
Ngai Ting Chan, Ming-Song Lee, Yidan Wang, Jacques Galipeau, Wan-Ju Li, Wei Xu
Summary: This study reveals that CTR9 regulates the osteochondral lineage differentiation of hMSCs by counteracting EZH2 activity and regulating BMP-2 expression. CTR9 knockdown in hMSCs leads to defects in osteogenic differentiation, which can be partially rescued by EZH2 inhibitors. CTR9 also regulates the secretion and function of BMP-2.
Article
Biotechnology & Applied Microbiology
Md Shahadat Hossan, Ethan Samuel Lin, Eleanor Riedl, Austin Stram, Eric Mehlhaff, Luke Koeppel, Jamie Warner, Inem Uko, Lori Mankowski Gettle, Sam Lubner, Stephanie M. McGregor, Wei Zhang, William Murphy, Jeremy D. Kratz
Summary: To accurately predict therapeutic responses in pancreatic and ampullary cancers, researchers have developed a low-volume screening assay and an automated alignment algorithm. By comparing the oligoclonal growth response with validated markers of response, subclonal populations and their relationship with treatment sensitivity were identified. This study is of great significance for utilizing cancer organoid models as biomarkers and advancing novel therapeutic development across different cancer types.
BIOENGINEERING-BASEL
(2023)
Article
Engineering, Biomedical
Junsu Yun, Samantha Robertson, Chanul Kim, Masatoshi Suzuki, William L. Murphy, Padma Gopalan
Summary: Decellularized plant scaffolds with innate uniaxial micropatterned topography were biofunctionalized with a copolymer to enable cell adhesion, growth, and alignment. Human muscle cells cultured on this biofunctionalized leaf scaffold showed aligned cell orientation and formation of muscle-like myotube structures, demonstrating potential for tissue engineering applications.
ACTA BIOMATERIALIA
(2023)
Article
Materials Science, Biomaterials
Jae Sung Lee, Ping Guo, Katarina Klett, MacGregor Hall, Krishna Sinha, Sudheer Ravuri, Johnny Huard, William L. Murphy
Summary: Autologous platelet-rich plasma (PRP) is a popular treatment for musculoskeletal tissue injuries due to its favorable safety profile and cost-effectiveness. However, the therapeutic efficacy of PRP for osteoarthritis and damaged cartilage has been inconsistent. In this study, the researchers developed a method to enhance the efficacy of PRP treatment by attenuating a growth factor known to inhibit cartilage repair. The results showed that VEGF-attenuated PRP significantly improved healing of rat OA cartilage compared to conventional PRP treatment or control group. This study provides a new PRP customization method that can be applied to various tissue repair processes.
BIOMATERIALS SCIENCE
(2022)
Article
Biochemical Research Methods
Elizabeth A. Aisenbrey, Elizabeth Torr, Hunter Johnson, Cheryl Soref, William Daly, William L. Murphy
Summary: Pericytes play a critical role in vascular functions and disease progression, making them ideal for disease modeling and regenerative cell therapy. This protocol outlines a rapid differentiation method for Pericytes from hiPSCs, while also generating endothelial progenitor cells.
Article
Materials Science, Biomaterials
David G. Belair, Jae Sung Lee, Anna Kellner, Johnny Huard, William L. Murphy
Summary: New TGF-beta 1 binding peptides that interfere with its binding to receptors were identified, showing potential for applications in treating fibrotic diseases and soft tissue repair. This approach could offer a design strategy for peptide antibodies based on ligand-receptor interactions.
BIOMATERIALS SCIENCE
(2021)