Article
Cell & Tissue Engineering
Yong-Hong Wang, Ya-Chao Tao, Dong-Bo Wu, Meng-Lan Wang, Hong Tang, En-Qiang Chen
Summary: Different storage solutions did not significantly affect cell behavior, while heterogeneity was prevalent in MSC populations and could limit cell application. Therefore, establishing a more precise standard for culture-expanded MSCs is necessary.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Tania Velletri, Carlo Emanuele Villa, Domenica Cilli, Bianca Barzaghi, Pietro Lo Riso, Michela Lupia, Raffaele Luongo, Alejandro Lopez-Tobon, Marco De Simone, Raoul J. P. Bonnal, Luca Marelli, Stefano Piccolo, Nicoletta Colombo, Massimiliano Pagani, Ugo Cavallaro, Saverio Minucci, Giuseppe Testa
Summary: This study introduces a new method for isolating and growing single cells from patients' metastatic ascites to establish 3D cultures referred to as sMOCS. By using single cell RNA sequencing, researchers were able to define the cellular composition of metastatic ascites and reproduce features of the original metastasis that cannot be observed in traditional 2D culture. This method provides a powerful tool for precision oncology in ovarian cancer by enriching informative cell subpopulations and analyzing their diversity at the functional and molecular level.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Construction & Building Technology
Feng Wang, Yue Xiao, Zongwu Chen, Peide Cui, Jian Liu, Ning Wang
Summary: This study investigated the relationship between filler morphology and pavement properties using morphological analysis and active adhesion test. Results show a close negative linear relationship between filler roundness and adhesion properties. It is recommended that the angularity of mineral filler should be less than 0.895 and the roundness should be less than 0.515 to ensure satisfactory active adhesion between aggregates and bitumen materials.
CONSTRUCTION AND BUILDING MATERIALS
(2022)
Article
Engineering, Biomedical
Yanan Liu, Chuping Zheng, Jie Liu
Summary: To improve the application of stem cell therapy, the authors used upconversion nanoparticles (UCNPs) as contrast agents to track human umbilical cord mesenchymal stem cells (HUCMSCs). The data showed that incubation of UCNPs allowed HUCMSCs to maintain their viability and differentiation ability, with smaller sized UCNPs demonstrating higher uptake capability. This study will contribute to the future engineering of stem cell applications using UCNPs.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2023)
Article
Engineering, Biomedical
Yongtao Wang, Yingjun Yang, Xinlong Wang, Naoki Kawazoe, Yingnan Yang, Guoping Chen
Summary: Cell adhesion area has a dominant influence on gene transfection efficiency, while cell spreading area hardly affects gene transfection. Different morphological factors influence cellular functions in gene transfection, suggesting new insights for designing functional biomaterials.
ACTA BIOMATERIALIA
(2021)
Article
Genetics & Heredity
Mohadese Hashem Boroojerdi, Vahid Hosseinpour Sarmadi, Maryam Maqbool, King-Hwa Ling, Pooria Safarzadeh Kozani, Pouya Safarzadeh Kozani, Rajesh Ramasamy
Summary: This study elucidates the regulatory mechanisms of mesenchymal stem cells (MSCs) on the proliferation of hematopoietic stem cells (HSCs), providing a potential approach for obtaining larger quantities of HSCs in vitro without the need for manipulating their differentiation potential.
Article
Chemistry, Medicinal
Yanran Yang, Daiyun Chen, Yilin Li, Jinghua Zou, Ruiqi Han, Hongkun Li, Jun Zhang
Summary: Puerarin has a positive impact on the proliferation and osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells, and promotes new bone formation. In a rat RME model, puerarin solution enhances osteogenesis.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2022)
Review
Cell Biology
Soudeh Ghafouri-Fard, Vahid Niazi, Mohammad Taheri, Abbas Basiri
Summary: Hematopoietic stem cells play a crucial role in both preserving the blood system and potentially differentiating into non-hematopoietic cells for the treatment of human disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Marley J. Dewey, Alan J. Collins, Aleczandria Tiffany, Victoria R. Barnhouse, Crislyn Lu, Vasiliki Kolliopoulos, Isha Mutrejag, Noreen J. Hickokh, Brendan A. C. Harley
Summary: The design of biomaterials for bone regeneration needs to reduce bacterial attachment and biofilm formation to prevent infection. This study investigates the ability of mineralized collagen biomaterials to resist infection and examines the effect of manuka honey on bacterial colonization, stem cell osteogenesis, and vasculature formation.
Article
Cell Biology
Fatemeh Tahmasebi, Parichehr Pasbakhsh, Shirin Barati, Soheila Madadi, Iraj R. Kashani
Summary: This study demonstrated that 21 days of PLX3397 treatment significantly reduced microglial cells, and MSCs transplantation decreased the number of astrocytes while increasing the population of oligodendrocytes, thereby promoting remyelination. Additionally, the results showed that PLX and MSC treatment elevated levels of remyelination in the corpus callosum of the cuprizone model of MS, indicating a potential therapeutic strategy for MS.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Engineering, Environmental
Gwang-Bum Im, Young Geon Kim, In-Seong Jo, Tae Yong Yoo, Sung-Won Kim, Hyun Su Park, Taeghwan Hyeon, Gi-Ra Yi, Suk Ho Bhang
Summary: This study investigated the effects of surfactant-free and surfactant-treated nanoparticles on cell viability and physiology. The results showed that the nanoparticles could scavenge reactive oxygen species and promote cell proliferation and adipogenic differentiation. However, the surfactant-treated nanoparticles exhibited higher cytotoxicity than the surfactant-free nanoparticles after long-term treatment.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Anatomy & Morphology
A. Thirumal Raj, Supriya Kheur, Ramesh Bhonde, Archana A. Gupta, Shankargouda Patil
Summary: This study assessed the effect of human MSC derived conditioned media on human cancer cell lines, identifying pro-carcinogenic and anti-carcinogenic molecules and effector pathways associated with different MSC types. The use of MSC-CM with pro-carcinogenic effect should be restricted in cancer patients regardless of the application nature.
Review
Biochemical Research Methods
Kai Weissenbruch, Enrico Lemma, Marc Hippler, Martin Bastmeyer
Summary: Micro-fabrication and nano-fabrication provide useful methods for addressing biological questions by mimicking physiological microenvironments. Two-dimensional patterns and three-dimensional scaffolds, obtained through techniques like lithography and direct laser writing, can shape hydrogels, synthetic polymers, and biologically derived materials for (single) cell culture. Micro-scaffolds mimicking cell niches have applications in stem cell renewal, differentiation, and lineage specification, as well as studying single cells in basic cell biology.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Alessia Giovanna Santa Banche Niclot, Elena Marini, Ivana Ferrero, Francesco Barbero, Elena Rosso, Ivana Fenoglio, Alessandro Barge, Augusto Pessina, Valentina Cocce, Francesca Paino, Katia Mareschi, Franca Fagioli
Summary: This study demonstrated that the conditioned medium isolated from MSCs loaded with PTX exhibited strong cytotoxic effects on OS cell lines. Additionally, the presence of nanoparticles with a size similar to extracellular vesicles and minimal doses of PTX were detected in the secretome.
Article
Multidisciplinary Sciences
Ula V. Jurkunas, Jia Yin, Lynette K. Johns, Sanming Li, Helene Negre, Kit L. Shaw, Lassana Samarakoon, Allison R. Ayala, Ahmad Kheirkhah, Kishore Katikireddy, Alex Gauthier, Stephan Ong Tone, Aaron R. Kaufman, Stacey Ellender, Diego E. Hernandez E. Rodriguez, Heather Daley, Reza Dana, Myriam Armant, Jerome Ritz
Summary: In this study, a novel xenobiotic-free, serum-free, antibiotic-free, two-step process was developed to treat unilateral limbal stem cell (LSC) deficiency. Cultivated autologous limbal epithelial cells (CALEC) were generated from limbal biopsies and expanded onto human amniotic membrane with rigorous quality control. The clinical trial showed preliminary feasibility and safety of CALEC transplants in patients with unilateral LSC deficiency, leading to a second phase of recruitment to collect longer term safety and efficacy data.
Article
Biotechnology & Applied Microbiology
M. Orth, T. Fritz, J. Stutz, C. Scheuer, B. Ganse, Y. Bullinger, J. S. Lee, W. L. Murphy, M. W. Laschke, M. D. Menger, T. Pohlemann
Summary: MCM + VB, loaded with VEGF and BMP-2, improves bone healing in atrophic non-unions by promoting angiogenesis and osteogenesis, as well as reducing early osteoclast activity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Andrew S. Khalil, Daniel Hellenbrand, Kaitlyn Reichl, Jennifer Umhoefer, Mallory Filipp, Joshua Choe, Amgad Hanna, William L. Murphy
Summary: Spinal cord injuries often have devastating consequences and limited therapeutic options. This study utilizes mineral-coated microparticles as efficient non-viral gene delivery vehicles to produce exogenous ChABC in spinal cord injuries, reducing CSPG deposition and improving motor function recovery.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Cell & Tissue Engineering
Anna Laura Nelson, GianLuca Fontana, Elizabeth Miclau, Mallory Rongstad, William Murphy, Johnny Huard, Nicole Ehrhart, Chelsea Bahney
Summary: This article reviews methods to enhance bone formation and improve bone repair, focusing on the challenges and solutions for activating the Wnt pathway through antibody-based therapeutics, synthetic Wnt surrogates, and scaffold doping. Additionally, emerging technologies such as mRNA therapy and CRISPR technology for clinical translation are discussed.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Youngki Yeo, Soo-Yoon Hwang, Jinwook Yeo, Jihun Kim, Jinhyuk Jang, Heung-Sik Park, Yong-Jin Kim, Duc Duy Le, Kyung Song, Moonhong Kim, Seunghwa Ryu, Si-Young Choi, Chan-Ho Yang
Summary: We report a technique for the spontaneous gathering of oxygen vacancies at strain-relaxed crack walls using flexoelectricity. Enhanced electronic conductance and a switchable diode-like feature were observed at the crack walls. The diffusivity of mobile defects in crack walls was determined to be 1.4 x 10-16 cm2/s, consistent with oxygen vacancy kinetics.
Article
Biotechnology & Applied Microbiology
Md Shahadat Hossan, Ethan Samuel Lin, Eleanor Riedl, Austin Stram, Eric Mehlhaff, Luke Koeppel, Jamie Warner, Inem Uko, Lori Mankowski Gettle, Sam Lubner, Stephanie M. McGregor, Wei Zhang, William Murphy, Jeremy D. Kratz
Summary: To accurately predict therapeutic responses in pancreatic and ampullary cancers, researchers have developed a low-volume screening assay and an automated alignment algorithm. By comparing the oligoclonal growth response with validated markers of response, subclonal populations and their relationship with treatment sensitivity were identified. This study is of great significance for utilizing cancer organoid models as biomarkers and advancing novel therapeutic development across different cancer types.
BIOENGINEERING-BASEL
(2023)
Article
Chemistry, Physical
Jiye Kim, Kyung-Yeon Doh, Seokho Moon, Chang-Won Choi, Hokyeong Jeong, Jaewon Kim, Wonseok Yoo, Kyungwook Park, Kyeongock Chong, Chunhyng Chung, Hanmei Choi, Si-Young Choi, Donghwa Lee, Jong Kyu Kim
Summary: We have achieved the conformal growth of sp2-hybridized few-layer h-BN on an array of Si-based nanotrenches using pulsed-mode MOCVD method. X-ray absorption fine structure spectroscopy and density functional theory calculations reveal that B-O bonds formed on the noncatalytic SiO2 surface serve as nucleation sites for the subsequent formation of mixed sp2- and sp3-hybridized BON2 and BN3. This allows for the conformal growth of few-layer sp2-hybridized h-BN with excellent step coverage, which can be used for advanced three-dimensional Si-based nanoscale architectures.
CHEMISTRY OF MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Yong-Jun Kwon, Youngki Yeo, Min-Su Kim, Yong-Jin Kim, Heung-Sik Park, Jaegyu Kim, Si-Young Choi, Chan-Ho Yang
Summary: Topological textures of ferroelectric polarizations have potential applications in future information technology. However, energy losses and deviations from stability can affect the symmetry and shape of these textures. In this study, we investigated the domain structure of bismuth tungsten oxide thin films grown on SrTiO3 substrates using advanced microscopy techniques. We discovered a hidden phase with a different polarization orientation that promotes the formation of flux closure domains, bringing us one step closer to an isotropic two-dimensional polar material.
Article
Chemistry, Multidisciplinary
Min-Yeong Choi, Chang-Won Choi, Dong-Yeong Kim, Moon-Ho Jo, Yong-Sung Kim, Si-Young Choi, Cheol-Joo Kim
Summary: We propose a method using sodium molybdate liquid alloys to control atomic defects at grain boundaries of monolayer MoS2. The Mo-rich environment in the alloys leads to the formation of Mo-polar 5|7 defects with a yield exceeding 95%. The photoluminescence (PL) intensity of VLS-grown polycrystalline MoS(2) films is enhanced due to the suppression of nonradiative recombination of charged excitons with donor-type defects of adsorbed Na elements.
Article
Chemistry, Multidisciplinary
Un Yang, Byunghwa Kang, Moon-Jung Yong, Dong-Hwan Yang, Si-Young Choi, Jung Ho Je, Seung Soo Oh
Summary: Through nanoscale confinement, evaporation, and solidification, the direct 3D printing of intact biopolymers is demonstrated, allowing precise control of molecular-scale structures and shapes. Different biopolymers can be patterned with desired size and geometry by controlling concentrations and molecular weights, and their 3D architectures are biologically active in various solvents.
Article
Multidisciplinary Sciences
Jennie A. M. R. Kunitake, Daniel Sudilovsky, Lynn M. Johnson, Hyun-Chae Loh, Siyoung Choi, Patrick G. Morris, Maxine S. Jochelson, Neil M. Iyengar, Monica Morrow, Admir Masic, Claudia Fischbach, Lara A. Estroff
Summary: Microcalcifications, primarily biogenic apatite, are important indicators in mammography for cancerous and benign breast pathologies. This study investigates the heterogeneity of 93 calcifications from 21 breast cancer patients using an omics-inspired approach, revealing physiological clusters, intratumor heterogeneity in carbonate content, enhanced trace metals in malignant-localized calcifications, and lower lipid-to-protein ratio in patients with poor composite outcome, suggesting potential clinical value in expanding research on calcification diagnostic metrics.
Article
Engineering, Biomedical
Junsu Yun, Samantha Robertson, Chanul Kim, Masatoshi Suzuki, William L. Murphy, Padma Gopalan
Summary: Decellularized plant scaffolds with innate uniaxial micropatterned topography were biofunctionalized with a copolymer to enable cell adhesion, growth, and alignment. Human muscle cells cultured on this biofunctionalized leaf scaffold showed aligned cell orientation and formation of muscle-like myotube structures, demonstrating potential for tissue engineering applications.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Soo-Yoon Hwang, Gyoung-Ja Lee, Yuzhao Qi, Michael Abraham Listyawan, Kyung Song, Youngho Kang, Jungho Ryu, Xiaoyan Lu, Min-Ku Lee, Si-Young Choi
Summary: In this study, a method to achieve outstanding thermal stability of converse piezoelectric constant in lead-free potassium sodium niobate (KNN)-based ceramics is proposed. The relationship between grain size and polar configuration is demonstrated, and improved thermal stability in fine grains is suggested. The thermal stability is realized in a device by successfully demonstrating the temperature dependence of piezoelectricity, making it possible to apply the lead-free piezoelectric ceramics as piezoelectric devices with high thermal stability.
MATERIALS HORIZONS
(2023)
Correction
Chemistry, Multidisciplinary
Ji-Sang An, Hae-Seung Lee, Pilgyu Byeon, Dongho Kim, Hyung Bin Bae, Si-Young Choi, Jungho Ryu, Sung-Yoon Chung
Summary: The study corrects the interstice-occupying dopant segregation at grain boundaries in perovskite oxide dielectrics and proposes a new class of ceramic capacitors.
ENERGY & ENVIRONMENTAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Ji-Sang An, Hae-Seung Lee, Pilgyu Byeon, Dongho Kim, Hyung Bin Bae, Si-Young Choi, Jungho Ryu, Sung-Yoon Chung
Summary: When foreign cations are doped into ABO(3)-type perovskite oxides, they usually replace A or B sites depending on their ionic size. This study uncovers the presence of indium dopants occupying square-planar interstices and charge-compensating Ba vacancies at grain boundaries in polycrystalline BaTiO3. This chemical heterogeneity inhibits grain-boundary migration and enables the attainment of fine-grain microstructures with unprecedented dielectric properties.
ENERGY & ENVIRONMENTAL SCIENCE
(2023)
