Activity enhancement of Microperoxide-11 immobilized on nanospheres with a nanosize Co3O4 core and a periodic mesoporous organosilica shell

Core-shell nanospheres with a nanosize Co3O4 core and a periodic mesoporous organosilica (PMO) shell were fabricated by the packing and self-assembly of nanosize Co3O4 particles-surfactantorganosilica complexes based on the S+I- pathway under basic conditions. The amount of Co3O4 nanoparticles is crucial to the formation of core-shell nanospheres. The obtained nanospheres possess a uniform particle size of 130 nm, a high surface area of 474.1 m(2) g(-1) and a large pore volume of 0.68 cm(3) g(-1), which are highly advantageous to the immobilization of Microperoxidase-11 (MP-11). The presence of Co3O4 nanoparticles could enhance the activity of immobilized MP-11, probably due to their peroxidase activity.