Article
Chemistry, Analytical
Kun Zhang, Guowei Ding, Fengqing Gai, Yafang Zhang, Xiaoni Wang, Zhiming Gou, Yujing Zuo
Summary: Autophagy is crucial for cellular health, and its dysfunction is associated with various conditions. The development of a polysiloxane-based probe that targets lysosomes and responds to pH changes offers a valuable tool for visualizing and studying autophagic processes. This innovative probe provides insights into the mechanisms of autophagy and potential therapeutic strategies for related diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Shan Wang, Bingya Wang, Lei Zhu, Ji-Ting Hou, Kang-Kang Yu
Summary: The ratiometric fluorescent probe presented in this study offers excellent photostability and chemostability for monitoring pH changes in living cells. Quantifying the down-regulation of intracellular pH during autophagy induced by various stimuli can provide valuable guidance for constructing autophagy models to study related pathological processes.
CHEMICAL COMMUNICATIONS
(2021)
Article
Spectroscopy
Xiao-Lei Xue, Yang Wang, Shaojin Chen, Kun-Peng Wang, Shu-Yan Niu, Qian-Shou Zong, Yi Jiang, Zhi-Qiang Hu
Summary: Monitoring intracellular pH using ratiometric fluorescent probes is important for studying biological processes including diseases. This study presents a new hemicyanine-based ratio-metric fluorescent probe with a hydroxyl group, which exhibits dual emission and can selectively respond to micro-environmental pH conditions in a range of 6.0 to 8.0. The probe can enter and accumulate in mitochondria and monitor pH microenvironmental conditions through fluorescence imaging in HepG2 cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Engineering, Environmental
Hui Liu, Haoyuan Li, Siyuan Xia, Shanshan Yu, Yujie Duan, Lei Wang, Hongxiang Zhu, Hui He
Summary: A cellulose nanocrystal-based upconversion ratiometric fluorescent nanoprobe was developed for pH monitoring and imaging by chemically bonding upconversion nanoparticles, fluorescein isothiocyanate, and rhodamine B acceptors using cellulose nanocrystals as a bridge. The probe addressed issues such as aggregation-caused quenching of dye, long donor-to-acceptor distance, and poor water dispersibility. It exhibited excellent selectivity, sensitivity, and visualization for intracellular pH levels of 5.0-7.4 with 980 nm excitation. The biocompatible probe inspired by chameleons enhanced penetration depth and showed promising results for pH monitoring and imaging in living cells and in vivo.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Physical
Jie Cui, Hailiang Nie, Xiaomeng Liang, Jie Bai, Xiaoling Zhang
Summary: Acidity in biological microenvironments plays a crucial role in physiology and pathology. This study developed a pyrene-derived ratiometric fluorescent probe that can sensitively and selectively monitor pH changes.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Applied
Zhang-Yi Li, Xiao-Ling Cui, Meng-Min Xiao, Jun-Ying Miao, Bao-Xiang Zhao, Zhao-Min Lin
Summary: BDMNP is a novel FRET-ICT-based pH probe that has shown high energy transfer efficiency and linear response in the pH range of 5.4-6.8 (pKa = 6.10), with high selectivity and sensitivity. It has been successfully applied to monitor lysosomal pH changes in living cells and can detect subtle pH changes in various environmental water samples.
Article
Chemistry, Analytical
Shuyi He, Steven Wu
Summary: This article reports a ratiometric temperature sensing technology based on polymer dots-Eu nanoparticles, which have unique characteristics such as high water solubility, good light stability, excellent biocompatibility, and low cost. The probe utilizes the red/blue fluorescence intensity ratio to monitor temperature change, and shows good accuracy and high sensitivity for temperature detection in vitro, making it suitable for monitoring temperature change in cells.
Review
Chemistry, Analytical
Subrata Munan, Rashmi Yadav, Niharika Pareek, Animesh Samanta
Summary: The pH levels in organelles differ from the cytoplasm and accurately mapping these pH levels is crucial for cellular homeostasis. Ratiometric fluorescent probes have become the forefront of research in targeting specific organelles and monitoring their dynamics. This review summarizes the recent advances in ratiometric pH probes and discusses the challenges in developing these probes for organelle-specific pH mapping.
Article
Chemistry, Analytical
Yan Yang, Rui Li, Sichun Zhang, Xinrong Zhang
Summary: A lysosome-targeting ratiometric fluorescent nanoprobe based on CPPs and QDs has been developed for monitoring pH fluctuation in living cells. The nanoprobe efficiently stains lysosomes in cells and enables ratiometric pH measurement. This method is expected to have wider applications in detecting subcellular active substances by combining different small molecular probes and functional peptides.
Review
Chemistry, Physical
S. Santhoshkumar, Manivannan Madhu, Wei-Bin Tseng, Wei-Lung Tseng
Summary: Gold nanoclusters (AuNCs) show great potential as ratiometric fluorescent probes due to their adjustable fluorescence wavelengths, biocompatibility, and resistance to photobleaching. By incorporating an additional fluorescence spectral peak, dual-emission AuNC-based probes have been developed to improve signal reproducibility. This review focuses on the preparation methods and applications of ratiometric fluorescent probes derived from AuNCs and other fluorescent nanomaterials or dyes for bioimaging. The challenges and future directions of using AuNCs for ratiometric bioimaging are also discussed.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Chuanping Zhang, Chenxiao Tang, Yuxiao Mei, Limin Zhang, Anwei Zhu, Yang Tian
Summary: An electrochemical method was reported for selectively sensing and accurately quantifying monoamine oxidase A (MAO-A) in the cortex and thalamus of a live mouse brain. Using this tool, it was found that MAO-A increased Ca2+ entry into neurons via the TPRM2 channel in the live mouse brain of an AD model.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Wei Shu, Jin Yu, Zhuohang Li, Xiaoqian Sun, Yunpeng Wang, Yulong Wu, Jie Qin, Yu Zhang, Haibin Xiao, Xiaoling Zhang
Summary: In this study, a novel mitochondria-targeted fluorescent probe SNRF was developed for monitoring mitochondrial pH changes. SNRF showed excellent molecular charge transfer performance and could accurately track pH variations during rapamycin-induced mitophagy.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Inorganic & Nuclear
Xiaoyong Zhai, Pengfei Feng, Nan Song, Guodong Zhao, Qingyi Liu, Liangliang Liu, Mei Tang, Yu Tang
Summary: A novel dual-functional ratiometric luminescent sensor, Eu2.2Tb97.8-TCA, was successfully fabricated for rapid and ultrasensitive detection of trace water in ethanol. The sensor achieved sensitive visual determination of water in ethanol with multiple readouts and could also monitor temperature with high accuracy and good recyclability. This material has potential applications in the design and synthesis of multifunctional materials.
INORGANIC CHEMISTRY FRONTIERS
(2022)
Article
Chemistry, Multidisciplinary
Lijuan Gui, Kaizhen Wang, Yuxin Wang, Jun Yan, Xian Liu, Jingxuan Guo, Ji Liu, Dawei Deng, Haiyan Chen, Zhenwei Yuan
Summary: Cell stress responses are involved in various diseases, and understanding the changes in lysosomal pH is crucial. A novel NIR ratiometric pH-responsive fluorescent probe (BLT) was developed to track lysosomal pH changes in live cells, mice, and zebrafish. The probe successfully distinguished pH variations induced by heat stress, oxidative stress, and acetaminophen-induced injury stress. This innovative probe holds significant value in studying drug-induced hepatotoxicity-related physiological changes in lysosomes.
CHINESE CHEMICAL LETTERS
(2023)
Review
Chemistry, Analytical
Guangze Yang, Yun Liu, Jisi Teng, Chun-Xia Zhao
Summary: The use of fluorescence labelling is common for tracking nanoparticles in drug delivery, but quantification can be challenging due to various factors. Forster resonance energy transfer (FRET) provides a distance ruler for probing NP drug delivery by utilizing energy transfer between donor and acceptor fluorophores. Different FRET approaches discussed in the article offer insights into the design of improved nanoparticles with controlled properties.
Article
Chemistry, Analytical
Gerhard J. Mohr, Heidrun Mueller
SENSORS AND ACTUATORS B-CHEMICAL
(2015)
Article
Chemistry, Analytical
Anuj K. Sharma, Gerhard J. Mohr
SENSORS AND ACTUATORS B-CHEMICAL
(2016)
Article
Chemistry, Analytical
Cindy Schaude, Eleonore Frohlich, Claudia Meindl, Jennifer Attard, Barbara Binder, Gerhard J. Mohr
Article
Chemistry, Analytical
Petar Kassal, Marko Zubak, Gregor Scheipl, Gerhard J. Mohr, Matthew D. Steinberg, Ivana Murkovic Steinberg
SENSORS AND ACTUATORS B-CHEMICAL
(2017)
Article
Chemistry, Analytical
Cindy Schaude, Claudia Meindl, Eleonore Froehlich, Jennifer Attard, Gerhard J. Mohr
Article
Chemistry, Analytical
Tinkara Mastnak, Aleksandra Lobnik, Gerhard J. Mohr, Matejka Turel
Article
Biochemical Research Methods
Anne Maerz, Sabine Trupp, Petra Roesch, Gerhard J. Mohr, Juergen Popp
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2012)
Article
Chemistry, Physical
Daniel Escudero, Sabine Trupp, Beate Bussemer, Gerhard J. Mohr, Leticia Gonzalez
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2011)
Article
Chemistry, Physical
Stefan Grund, Tristan Doussineau, Dagmar Fischer, Gerhard J. Mohr
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2012)
Article
Biochemical Research Methods
Krzysztof Ryszard Idzik, Piotr J. Cywinski, Charles G. Cranfield, Gerhard J. Mohr, Rainer Beckert
JOURNAL OF FLUORESCENCE
(2011)
Article
Materials Science, Ceramics
Antje Kriltz, Christin Loeser, Gerhard J. Mohr, Sabine Trupp
JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
(2012)
Article
Nanoscience & Nanotechnology
Alejandro Lapresta-Fernandez, Tristan Doussineau, Silvio Dutz, Frank Steiniger, Artur J. Moro, Gerhard J. Mohr
Article
Chemistry, Analytical
Gerhard J. Mohr
SENSORS AND ACTUATORS B-CHEMICAL
(2018)
Article
Chemistry, Analytical
Tinkara Mastnak, Aleksandra Lobnik, Gerhard J. Mohr, Matjaz Finsgar
Article
Materials Science, Textiles
Cindy Schaude, Gerhard J. Mohr
FASHION AND TEXTILES
(2017)