期刊
JOURNAL OF MASS SPECTROMETRY
卷 47, 期 7, 页码 932-939出版社
WILEY
DOI: 10.1002/jms.3005
关键词
multiple-reaction monitoring; amino acids; dansyl
资金
- QUASIORA project of the Calabria APQ-RAC network
- Italian National Project, MIUR-PRIN [2009XZ7XAB_003]
5-Dimethylamino-1-sulfonyl naphthalene (DNS, commonly referred as dansyl) is a functionality, bearing well-established properties in directing the fragmentation, by mass spectrometry (MS), of the corresponding ionized sulfonylated derivatives. This property is shared also by its labeled analogs. The use of d0/d6 DNS derivatives is now exploited in the application of the well-established isotope dilution mass spectrometric approach in the assay of complex mixtures. A new method for the quantitation of amino acids (AAs) in beverages is therefore presented, which relies on liquid chromatographic separation of their N-dansylated derivatives followed by comparative electrospray tandem MS/MS of the d0/d6 isobaric mixtures. Labeled and unlabeled DNS derivatives of the selected AAs are readily available by microwave-assisted synthetic protocols. The novelty of the method is represented by the use of heavy and light DNS-isotopologue providing suitable reporter groups. Multiple-reaction monitoring has been applied in the assay of AAs in wine, pineapple juice and bergamot juice with good-to-excellent results as proved by both relative standard deviation, lower than 15%, and by the accuracy values in the range 90110%. Copyright (C) 2012 John Wiley & Sons, Ltd.
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