期刊
JOURNAL OF MASS SPECTROMETRY
卷 44, 期 7, 页码 1017-1025出版社
WILEY
DOI: 10.1002/jms.1573
关键词
flavonoids characterization; mass spectrometry; diglycosides; LC/MS; Citrus juice; rutinosides; neohesperidosides
资金
- Universidad del Pais Vasco/Euskal Herriko Unibertsitatea [13468/2001]
- Gobierno Vasco/Eusko Jaurlaritza
- Universidad del Pais Vasco/Euskal Herriko Unibertsitate
Fifteen flavonoid O-diglycosides with different interglycosidic linkage isomery and glycosylation position have been studied in order to analyze their fragmentation patterns. Initial separation was carried out using high performance liquid chromatography with diode array detection (HPLC/DAD) coupled to an electrospray ionization (ESI) interface and a triple quadrupole mass spectrometer. Some useful differences in their MS spectra have been found and discussed. As it has already been reported, [Y*](+)/[Y-0](+) ratio for flavanones and [Y-1](+)/[Y-0](+) ratio for other flavonoids is specific for each isomeric interglycosidic linkage. In this work it has also been observed that the abundance of these ions is dependent on the position of glycosylation. On the basis of these differences, systematic guidelines for our experimental conditions have been proposed for the differentiation of not only isomeric interglycosidic linkage but also glycosylation position using collision-induced dissociation MS/MS (CID-MS/MS) spectra in positive mode. These results have been successfully applied for the characterization of three diglycosyl flavonoids found in Citrus fruit juices and these conclusions have also been extrapolated for characterizing two triglycosides in the same fruits. Copyright (C) 2009 John Wiley & Sons, Ltd.
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