Article
Nutrition & Dietetics
Mario C. Chang, Stephen J. Staklinski, Vinay R. Malut, Geraldine L. Pierre, Michael S. Kilberg, Matthew E. Merritt
Summary: The natural amino acid asparagine (Asn) is essential for cell function and proliferation. While healthy cells can synthesize Asn, cancer and genetically diseased cells rely on extracellular sources. Deficiency in asparagine synthetase (ASNS), the enzyme responsible for Asn synthesis, leads to congenital microcephaly, seizures, and brain atrophy. In this study, metabolomics analysis of ASNS-deficient cells revealed disruptions in various metabolites, along with decreased TCA cycle intermediates and anaplerotic substrates in response to Asn deprivation. Pantothenate, phenylalanine, and aspartate were identified as potential biomarkers of Asn deprivation, suggesting the possibility of a novel diagnostic method for ASNS deficiency using targeted biomarker analysis from a blood draw.
Article
Meteorology & Atmospheric Sciences
Shaopeng Gao, Baiqing Xu, Xiaoyan Zheng, Xin Wan, Xiaolong Zhang, Guangming Wu, Zhiyuan Cong
Summary: This study developed an alternative analytical procedure for amino acids in rain samples using gas chromatography coupled with mass spectrometry, with low detection limits and applicability. The relative abundances of amino acids in rain samples were found to be similar and likely originated from biomass burning. The method also shows potential for future analysis of amino acids in glacier ice cores.
ATMOSPHERIC RESEARCH
(2021)
Article
Plant Sciences
Liang Yang, Zhouling Wei, Marc Valls, Wei Ding
Summary: Metabolic analyses were used to identify metabolite differences in tobacco xylem sap in response to Ralstonia pseudosolanacearum infection, revealing metabolites that can promote bacterial growth.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jingjing Ma, Kun Chen, Yun Ding, Xiao Li, Qiming Tang, Bo Jin, Ruben Y. Luo, Sheeno Thyparambil, Zhi Han, C. James Chou, Ashlee Zhou, James Schilling, Zhiguang Lin, Yan Ma, Qing Li, Mengxue Zhang, Karl G. Sylvester, Seema Nagpal, Doff B. McElhinney, Xuefeng B. Ling, Bobin Chen
Summary: This study developed two logistic regression models using metabolic markers in cerebrospinal fluid (CSF) for the diagnosis and differential diagnosis of PCNSL. These models provide valuable insights and hold promise for the future development of a non-invasive and reliable diagnostic tool for PCNSL.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Plant Sciences
Dhiraj Dokwal, Jean-Christophe Cocuron, Ana Paula Alonso, Rebecca Dickstein
Summary: Medicago truncatula undergoes metabolic changes in response to phosphorus deprivation, with a decrease in organic acids and phosphorylated metabolites in leaves and an increase in sugars and amino acids in the whole plant.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Agricultural Engineering
R. S. Sowmya, Vishal G. Warke, Girish B. Mahajan, Uday S. Annapure
Summary: Amino acids promote the growth of coriander by improving plant metabolism. This study analyzed the impact of amino acids solution on coriander cultivated hydroponically under different light conditions. Results showed that the incorporation of amino acids into the nutrient solutions significantly affected the growth, elemental content, chlorophyll, carotenoid, amino acids, and essential oils profile of coriander. The use of 100 ppm amino acids solution resulted in morphological changes, increased nutrient content, enhanced chlorophyll and carotenoid content, and higher accumulation of aromatic compounds.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Food Science & Technology
Xin Guo, Yongqin Wang, Shiling Lu, Jingyun Wang, Huihui Fu, Bingyan Gu, Bing Lyu, Qingling Wang
Summary: Changes in proteolysis, protein oxidation, flavor, color, and texture in dry-cured mutton ham were investigated during storage for up to 180 days. Significant alterations were observed in protein content, amino acids, and flavor compounds, with key changes occurring after 90 days of storage.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Chunning Luan, Min Zhang, Sakamon Devahastin, Yaping Liu
Summary: By utilizing two-step fermentation with LAB and Saccharomyces cerevisiae, the quality of horseradish sauce can be improved, with increased amino acid content, antioxidant activities, and flavorful compounds. Evaluation using electronic tongue and sensory analysis can determine the overall sensory quality of the sauce.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Nutrition & Dietetics
Tianxiao Zhou, Kaige Yang, Yinjie Ma, Jin Huang, Wenchang Fu, Chao Yan, Xinyan Li, Yan Wang
Summary: The important metabolic characteristics of cancer cells include increased fat production and changes in amino acid metabolism. Local catabolism of tryptophan and arginine is associated with weakened anti-tumor immunity. Understanding the metabolism of amino acids and fatty acids within cells is crucial. In this study, a method for simultaneous analysis of 64 metabolites was established, highlighting the metabolic effects of various fatty acids on lung cancer cells.
Article
Toxicology
Cong Hu, Hongwei Li, Linjing Wu, Jiaqun Ke, Xuechun Yu, Yinhua Xiong, Xilan Tang
Summary: The study utilized GC-QqQ-MS/MS technology to investigate the changes in amino acid levels caused by TP in rats, and linked the related amino acids with metabolic pathways to identify biomarkers for TP-induced liver toxicity.
HUMAN & EXPERIMENTAL TOXICOLOGY
(2021)
Article
Food Science & Technology
Sonia Carabetta, Rosa Di Sanzo, Salvatore Fuda, Adele Muscolo, Mariateresa Russo
Summary: A predictive model correlating amino acid profiles to aromatic compounds was built to understand the biochemistry of honey's organoleptic properties. The study used a selection of unifloral honeys produced in Calabria, South Italy, and developed a new methodology using HILIC-UHPLC-ESI-MS/MS and HS-SPME-GC-MS combined with multivariate processing. The results showed strong positive correlations between specific amino acids and different classes of aroma compounds.
Article
Chemistry, Analytical
Ines Domingo-Orti, Patricia Ferrer-Torres, Ana Arminan, Maria J. Vicent, Antonio Pineda-Lucena, Martina Palomino-Schaetzlein
Summary: Studying metabolism can help understand the relationship between mitochondrial activity and various diseases. This study developed a method to characterize mitochondrial metabolism in healthy, tumoral, and metastatic tissues and discovered significant differences in metabolites between these tissues.
ANALYTICAL CHEMISTRY
(2023)
Article
Ophthalmology
Hanying Wang, Shu Li, Chingyi Wang, Yihan Wang, Junwei Fang, Kun Liu
Summary: This study aimed to investigate the differences in metabolites and metabolic pathways between patients with proliferative diabetic retinopathy (PDR) and nondiabetic controls in plasma and vitreous, as well as the relationship between plasma and vitreous metabolic profiles. The study found that there were significant differences in metabolomes between patients with PDR and controls in both plasma and vitreous. The pathway analysis revealed altered metabolic pathways, and there were positive correlations between vitreous and plasma in a few metabolic features.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Horticulture
Xiaoya Tao, Qiong Wu, Suqing Huang, Beiwei Zhu, Feng Chen, Bin Liu, Luyun Cai, Linchun Mao, Zisheng Luo, Li Li, Tiejin Ying
Summary: This research reveals that the plant hormone abscisic acid (ABA) plays a crucial role in the development and ripening of tomato fruit. Exogenous ABA was found to accelerate tomato fruit ripening and promote the accumulation of primary metabolites, such as sugars, organic acids, and amino acids. Additionally, ABA was shown to stimulate the expression of key genes involved in sugar and organic acid metabolism.
SCIENTIA HORTICULTURAE
(2022)
Article
Biochemistry & Molecular Biology
Svetlana Baskal, Alexander Bollenbach, Dimitrios Tsikas
Summary: The study demonstrates that reversing the order of two-step derivatization allows discrimination and simultaneous analysis of amino acids Cit and Orn, as well as hCit and Lys. Procedure B also results in the formation of additional reaction products, and possible mechanisms for their formation are proposed.