期刊
JOURNAL OF MAGNETIC RESONANCE
卷 210, 期 2, 页码 246-259出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2011.03.015
关键词
Solid-state NMR; REDOR; Order parameter; Dynamic measurement
资金
- Swiss National Science Foundation [200020_124611]
- ETH
- Swiss National Science Foundation (SNF) [200020_124611] Funding Source: Swiss National Science Foundation (SNF)
The accurate experimental determination of dipolar-coupling constants for one-bond heteronuclear dipolar couplings in solids is a key for the quantification of the amplitudes of motional processes. Averaging of the dipolar coupling reports on motions on time scales up to the inverse of the coupling constant, in our case tens of microseconds. Combining dipolar-coupling derived order parameters that characterize the amplitudes of the motion with relaxation data leads to a more precise characterization of the dynamical parameters and helps to disentangle the amplitudes and the time scales of the motional processes, which impact relaxation rates in a highly correlated way. Here. we describe and characterize an improved experimental protocol - based on REDOR - to measure these couplings in perdeuterated proteins with a reduced sensitivity to experimental missettings. Because such effects are presently the dominant source of systematic errors in experimental dipolar-coupling measurements, these compensated experiments should help to significantly improve the precision of such data. A detailed comparison with other commonly used pulse sequences (T-MREV, phase-inverted CP, R18(2)(5), and R18(1)(7)) is provided. (C) 2011 Elsevier Inc. All rights reserved.
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