期刊
JOURNAL OF MAGNETIC RESONANCE
卷 213, 期 1, 页码 69-75出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2011.08.038
关键词
NMR spectroscopy; Pulsed field gradients (PFG); Diffusion ordered spectroscopy (DOSY); Resolution overlap; 3D heteronuclear DOSY; BEST-HMQC; Carbon-13 spectroscopy
资金
- Department of Biotechnology, Ministry of Science & Technology India [BT/PR9446/BRB/10/558/2007]
A novel diffusion-edited 3D NMR experiment that incorporates a BEST-HMQC pulse sequence in its implementation is presented. Heteronuclear 3D DOSY NMR experiments are useful in elucidating the diffusion coefficients of individual constituents of a mixture, especially in cases where the proton NMR 2D DOSY spectra show considerable overlap. The present 3D BEST-DOSY pulse sequence provides a more sensitive and less time-consuming alternative to standard 3D HMQC-DOSY experiments. Cleanly separated subspectra of individual mixture components are obtained, leading to the determination of diffusion coefficients with better accuracy. The feasibility of the technique is demonstrated on a mixture of amino acids, on a mixture of small molecules with similar diffusion coefficients, and on a complex mixture with large dynamic range (commercial gasoline). The implications of using adiabatic decoupling schemes and band-selective shaped pulses for selective BEST-DOSY experiments on proteins are also discussed. (C) 2011 Elsevier Inc. All rights reserved.
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