期刊
JOURNAL OF LUMINESCENCE
卷 129, 期 9, 页码 926-930出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jlumin.2009.03.017
关键词
Quantum dots; CdTe; Immuno-fluorescence; Prostate cancer cell; Direct labeling; Indirect labeling
类别
资金
- National Science Foundation of China [20875011]
- Liaoning Province Education Department [05L441]
- Liaoning Province Science & Technology Department [20072063]
L-glutathione (GSH) stabilized CdTe quantum dots (QDs) were directly prepared in aqueous solution. The as-prepared QDs were linked to prostate-specific antigen (PSA) for the direct labeling and linked to immunoglobulin G (IgG) for the indirect labeling of fixed prostate cancer cells. The results indicated that QD-based probes were ideal fluorescent markers with excellent spectral properties and photostability and much better than organic dyes making them very suitable in target detection. Meanwhile, the indirect labeling showed much better specificity than the direct labeling. Furthermore, the prepared CdTe QDs did not show detectable effect on cell growth after having cultured for three days, which suggested that the L-glutathione capped CdTe had scarcely cytotoxicity. (C) 2009 Elsevier B.V. All rights reserved.
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