期刊
JOURNAL OF LUMINESCENCE
卷 128, 期 12, 页码 1969-1974出版社
ELSEVIER
DOI: 10.1016/j.jlumin.2008.06.008
关键词
Fluorescence; Quenching; Nanoparticles; BSA; Adsorption
类别
资金
- CSIR [01(2057)/06/EMR-II]
Gold nanoparticles of different sizes have been synthesized using sodium citrate as a reducing agent for tetrachloroauric (III) acid. The formed gold nanoparticles have been characterized by the UV-visible and transmission electron microscopy (TEM) measurements. The different sized gold nanoparticles have been used to study the interaction with model transport protein, bovine serum albumin (BSA). Experimental results reveal that BSA molecules adsorbed on the metallic surfaces, suffer strong quenching of their fluorescence and the rate of quenching efficiency is different for different particle size. The analysis of the quenching results has been performed in terms of the Stern-Volmer equation. The mechanism of quenching of fluorescence has been explained. The extent of adsorption of BSA on the gold nanoparticles has been estimated. (c) 2008 Elsevier B.V. All rights reserved.
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