期刊
JOURNAL OF LIPID RESEARCH
卷 55, 期 9, 页码 1970-1980出版社
ELSEVIER
DOI: 10.1194/jlr.D048660
关键词
oocyte; embryo; time-of-fl ight secondary ion mass spectrometry; mass spectrometry imaging
资金
- Analytical Trust Fund of the Royal Society of Chemistry
- UK Engineering and Physical Sciences Research Council (EPSRC) [EP/G045623]
- Engineering and Physical Sciences Research Council [EP/G045623/1, EP/K01353X/1] Funding Source: researchfish
- EPSRC [EP/K01353X/1, EP/G045623/1] Funding Source: UKRI
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging has been used for the direct analysis of single intact Xenopus laevis embryo surfaces, locating multiple lipids during fertilization and the early embryo development stages with subcellular lateral resolution (similar to 4 mu m). The method avoids the complicated sample preparation for lipid analysis of the embryos, which requires selective chemical extraction of a pool of samples and chromatographic separation, while preserving the spatial distribution of biological species. The results show ToF-SIMS is capable of profiling multiple components (e.g., glycerophosphocholine, SM, cholesterol, vitamin E, diacylglycerol, and triacylglycerol) in a single X. laevis embryo. We observe lipid remodeling during fertilization and early embryo development via time course sampling. The study also reveals the lipid distribution on the gamete fusion site.jlr The methodology used in the study opens the possibility of studying developmental biology using high resolution imaging MS and of understanding the functional role of the biological molecules.
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