Article
Multidisciplinary Sciences
Varnavas D. Mouchlis, Daiki Hayashi, Alexis M. Vasquez, Jian Cao, J. Andrew McCammon, Edward A. Dennis
Summary: Lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) associates with lipoproteins in human plasma and hydrolyzes oxidized phospholipids. The mechanism of enzyme-membrane association and substrate specificity were studied using lipidomics and mass spectrometry techniques.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Xue Jiang, Jie Xu, Xiwa Hao, Jing Xue, Ke Li, Aoming Jin, Jinxi Lin, Xia Meng, Lemin Zheng, Yongjun Wang
Summary: The study found that elevated levels of Lp(a) are associated with unfavorable functional outcomes in patients with ischemic stroke. Furthermore, the combination of high Lp(a) and high Lp-PLA(2) levels showed the highest incidence of unfavorable functional outcomes at 3 months and 1 year after ischemic stroke.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Multidisciplinary Sciences
Ian Kwong Yun Phoon, Yi Ling Eileen Koh, Xiaoxuan Guo, Sankari Usha, Ngiap Chuan Tan
Summary: Recent research in Singapore compared fasting and non-fasting lipid profiles in Asian patients with type-2 diabetes mellitus and dyslipidaemia. The study found that the non-fasting lipid profile was similar to the fasting profile in patients taking statin medication, suggesting that non-fasting lipid profiles can be used to assess cholesterol treatment status in this population.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Eric Yuk Fai Wan, Esther Yee Tak Yu, Weng Yee Chin, Christie Sze Ting Lau, Anna Hoi Ying Mok, Yuan Wang, Ian Chi Kei Wong, Esther Wai Yin Chan, Cindy Lo Kuen Lam
Summary: This study found positive linear associations between variability of LDL-C and TC/HDL-C ratio and the risk of kidney disease in patients with type 2 diabetes, but no association was found with triglyceride variability. Each mmol/L increase in LDL-C variability was associated with higher risks in incident kidney disease, renal function decline, and end-stage renal disease. Variability in lipid parameters could be a useful predictor for kidney diseases in diabetic patients.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Juan R. Ulloque-Badaracco, Ali Al-kassab-Cordova, Enrique A. Hernandez-Bustamante, Esteban A. Alarcon-Braga, Miguel Huayta-Cortez, Ximena L. Carballo-Tello, Rosa A. Seminario-Amez, Percy Herrera-Anazco, Vicente A. Benites-Zapata
Summary: This systematic review and meta-analysis reveals that increased levels of ApoB and the ApoB/ApoA1 ratio are associated with metabolic syndrome (MetS), while decreased levels of ApoA1 and Lp(a) are associated with MetS. These lipid markers may serve as potential indicators for identifying individuals at risk of developing MetS, but further research is needed to understand the underlying mechanisms of these associations.
LIPIDS IN HEALTH AND DISEASE
(2023)
Review
Pharmacology & Pharmacy
Yi-Ting Huang, Li-Ting Ho, Hsin-Yin Hsu, Yu-Kang Tu, Kuo-Liong Chien
Summary: PCSK9 inhibitors have significant effects on reducing LDL-C, ApoB, and Lp(a) levels in statin-treated patients. Evolocumab 140 mg Q2W showed significantly larger reductions in these lipids.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Peripheral Vascular Disease
Rishi Rikhi, Harpreet S. Bhatia, Christopher L. Schaich, Nicklaus Ashburn, Michael Y. Tsai, Erin D. Michos, Parag A. Chevli, David M. Herrington, Sotirios Tsimikas, Michael D. Shapiro
Summary: This study examined the relationship between Lp(a) and hypertension with cardiovascular outcomes in a large multiethnic cohort. The results showed that individuals with elevated Lp(a) and hypertension had a significantly increased risk for cardiovascular disease.
Article
Public, Environmental & Occupational Health
Mika Ala-Korpela, Siyu Zhao, Marjo-Riitta Jarvelin, Ville-Petteri Makinen, Pauli Ohukainen
Summary: Quantitative analysis of lipoproteins using NMR spectroscopy is common in large-scale studies, with a methodology allowing for comprehensive characterization of lipoprotein subclasses. In this study, serum samples from two Finnish birth cohorts were analyzed using NMR, revealing the important relationship between lipoprotein particle concentrations and size. The findings suggest that lipoprotein particles are under strict metabolic constraints, impacting their composition and concentrations.
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY
(2021)
Article
Endocrinology & Metabolism
Siqian Gong, Shaofeng Huo, Yingying Luo, Yufeng Li, Yumin Ma, Xiuting Huang, Mengdie Hu, Wei Liu, Rui Zhang, Xiaoling Cai, Lingli Zhou, Ling Chen, Qian Ren, Simin Zhang, Yu Zhu, Xiuying Zhang, Jing Chen, Jing Wu, Xianghai Zhou, Xu Lin, Xueyao Han, Linong Ji
Summary: Variants of the SORBS1 gene are associated with impaired glucose regulation and HDL cholesterol levels in the Chinese Han population, but not with diabetic kidney disease.
DIABETES-METABOLISM RESEARCH AND REVIEWS
(2022)
Article
Cardiac & Cardiovascular Systems
Kazuki Suruga, Toru Miyoshi, Kazuhiko Kotani, Keishi Ichikawa, Takashi Miki, Kazuhiro Osawa, Kentaro Ejiri, Hironobu Toda, Kazufumi Nakamura, Hiroshi Morita, Hiroshi Ito
Summary: This study demonstrated a significant association between elevated oxHDL/apoAI ratio and coronary plaque characteristics, such as HRP and significant stenosis, as determined by coronary CT angiography. These findings suggest that higher oxHDL/apoAI ratio may predict cardiovascular events in patients with suspected stable CAD.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Jakub Siudut, Joanna Natorska, Ewa Wypasek, Lukasz Wiewiorka, Elzbieta Ostrowska-Kaim, Sylwia Wisniowska-Smialek, Krzysztof Plens, Piotr Musialek, Jacek Legutko, Anetta Undas
Summary: This study investigated the impact of apolipoproteins and oxidized low-density lipoprotein on the prothrombotic fibrin clot phenotype in patients with aortic stenosis. The results showed positive associations between apolipoproteins and oxidized low-density lipoprotein with fibrin clot properties, as well as negative associations with fibrinolytic capacity. This suggests that elevated lipoproteins may play an important role in promoting blood coagulation.
Article
Medicine, General & Internal
Wei Wang, Zhe Yuan Chen, Fen Yan Lv, Mei Tu, Xiu Li Guo
Summary: This study evaluates the correlation of serum HDL-c and apolipoprotein A1 (APOA1) with bone metabolism in Chinese postmenopausal women with type 2 diabetes mellitus (T2DM). The results show that APOA1 is more strongly associated with bone metabolism compared to HDL-c.
FRONTIERS IN MEDICINE
(2023)
Article
Medicine, Research & Experimental
Boyan Liu, Min Zhao, Junli Xue, Qianqian Gu, Xiaoyi Zhang, Shucun Qin
Summary: The study demonstrated that inhalation of 4% H-2 reduced the accumulation of OxPLs in rats and altered the components of HDL, enhancing its antioxidative capacity. Additionally, H-2 significantly regulated the activity and expression of HDL-associated enzymes. These findings suggest that H-2 may be used as a therapeutic approach for diseases related to lipid peroxidation caused by oxidative stress.
Article
Endocrinology & Metabolism
Pei Yang, DongDong Li, Xiaokang Li, Zongbiao Tan, Huan Wang, Xiaona Niu, Yang Han, Cheng Lian
Summary: This study investigated the relationship between HDL-C levels and intertrochanter bone mineral density. The results showed a negative correlation between HDL-C and intertrochanter BMD. Subgroup analysis revealed negative correlations among 60-70-year-olds, non-Hispanic whites, and obese individuals. The relationship between HDL-C and intertrochanter BMD was an inverted U-shaped curve in men and hormone users, and a U-shaped curve in other Hispanic and premenopausal individuals.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Cardiac & Cardiovascular Systems
Hsiang-Chun Lee, Alexander Akhmedov, Chu-Huang Chen
Summary: This article mainly introduces the composition, function, and association of very-low-density lipoprotein (VLDL) with cardiometabolic diseases. Large VLDL particles are correlated with atherosclerosis, insulin resistance, and diabetes incidence. The most electronegative VLDL subclass is highly cytotoxic to endothelial cells and may contribute to coronary heart disease and atrial fibrillation development.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Hans C. Leier, Jules B. Weinstein, Jennifer E. Kyle, Joon-Yong Lee, Lisa M. Bramer, Kelly G. Stratton, Douglas Kempthorne, Aaron R. Navratil, Endale G. Tafesse, Thorsten Hornemann, William B. Messer, Edward A. Dennis, Thomas O. Metz, Eric Barklis, Fikadu G. Tafesse
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Daiki Hayashi, Varnavas D. Mouchlis, Edward A. Dennis
Summary: This study reveals the unique substrate selectivity of human phospholipase A2s (PLA2) for different fatty acids, discussing the substrate preferences and active site properties of cytosolic cPLA2 enzymes, calcium-independent iPLA2 enzymes, and secreted sPLA2 enzymes.
JOURNAL OF LIPID RESEARCH
(2021)
Letter
Multidisciplinary Sciences
Harald C. Koefeler, Thomas O. Eichmann, Robert Ahrends, John A. Bowden, Niklas Danne-Rasche, Edward A. Dennis, Maria Fedorova, William J. Griffiths, Xianlin Han, Juergen Hartler, Michal Holcapek, Robert Jirasko, Jeremy P. Koelmel, Christer S. Ejsing, Gerhard Liebisch, Zhixu Ni, Valerie B. O'Donnell, Oswald Quehenberger, Dominik Schwudke, Andrej Shevchenko, Michael J. O. Wakelam, Markus R. Wenk, Denise Wolrab, Kim Ekroos
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Daiki Hayashi, Varnavas D. Mouchlis, Edward A. Dennis
Summary: Glycerophospholipids are major components of cell membranes with diverse fatty acyl chain compositions and polar head groups. Phospholipase A(2) enzymes play a critical role in metabolism by hydrolyzing glycerophospholipids, and different human PLA(2) enzymes show distinct preferences for sn-1 acyl chain linkages.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Article
Multidisciplinary Sciences
Varnavas D. Mouchlis, Daiki Hayashi, Alexis M. Vasquez, Jian Cao, J. Andrew McCammon, Edward A. Dennis
Summary: Lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) associates with lipoproteins in human plasma and hydrolyzes oxidized phospholipids. The mechanism of enzyme-membrane association and substrate specificity were studied using lipidomics and mass spectrometry techniques.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Maria A. Theodoropoulou, Anastasia Psarra, Martin Erhardt, Aikaterini Nikolaou, Anna-Dimitra D. Gerogiannopoulou, Dimitra Hadjipavlou-Litina, Daiki Hayashi, Edward A. Dennis, Andrea Huwiler, George Kokotos
Summary: The search for new drugs that can regulate the production of prostaglandin E-2 (PGE(2)) is crucial in the treatment of inflammatory diseases. In this study, we synthesized and evaluated a series of compounds, and identified N-acylated and N-alkylated 2-aminobenzothiazoles as potential leads. These compounds showed significant inhibition of PGE(2) generation in rat mesangial cells and demonstrated better anti-inflammatory activity than indomethacin. The findings suggest that N-acylated or N-alkylated 2-aminobenzothiazoles could be promising candidates for regulating PGE(2) formation.
Article
Multidisciplinary Sciences
Loren M. DeVito, Edward A. Dennis, Barbara B. Kahn, Gerald Shulman, Joseph L. Witztum, Sudeshna Sadhu, Joseph Nickels, Matthew Spite, Susan Smyth, Sarah Spiegel
Summary: Recent research has revealed the cellular and molecular functions of bioactive lipids as signaling molecules in regulating inflammation and lipid metabolism, which can help identify mechanisms targeted to prevent or treat cardiometabolic conditions.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Edward A. Dennis
Summary: Lipids play important roles in chronic diseases, but defining the substrate specificity of lipid metabolism enzymes is challenging. This research focuses on how membranes interact with enzymes to regulate cell signaling and metabolic pathways. By using substrate lipidomics and molecular dynamics simulations, the study reveals the link between enzyme specificity and hydrophobic binding subsites for membrane phospholipid substrates.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Daiki Hayashi, Varnavas D. Mouchlis, Seika Okamoto, Tomoka Namba, Liuqing Wang, Sheng Li, Shuji Ueda, Minoru Yamanoue, Hirofumi Tachibana, Hiroyuki Arai, Hitoshi Ashida, Edward A. Dennis, Yasuhito Shirai
Summary: Alpha-tocopherol (alpha Toc), the active form of vitamin E, has both antioxidant and non-antioxidant effects. It has been discovered that the membrane-bound 67 kDa laminin receptor (67LR) serves as a receptor for alpha Toc, mediating the non-antioxidant effects such as DGK alpha activation. This study provides the first evidence of a membrane receptor for alpha Toc and one of the underlying mechanisms of its non-antioxidant function.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Edward A. Dennis
Summary: The translation describes the author's laboratory research on lipids, focusing on phospholipases and lipidomics, and the evolution of the lipid field. The author also discusses the parallel side stories and activities that influenced their laboratory research and contributed to the development of the LIPID MAPS initiative.
Review
Chemistry, Multidisciplinary
Varnavas D. Mouchlis, Edward A. Dennis
Summary: Water-soluble proteins and membrane-bound proteins interact with membrane surfaces and bind specific lipid molecules. Phospholipases are important enzymes in biological membranes that catalyze hydrolysis reactions by interacting with membranes and extracting phospholipid substrates. The association of phospholipases with membranes induces conformational changes and stabilizes the enzymes in an active state.
ACCOUNTS OF CHEMICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Matthew J. Conroy, Robert M. Andrews, Simon Andrews, Lauren Cockayne, Edward A. Dennis, Eoin Fahy, Caroline Gaud, William J. Griffiths, Geoff Jukes, Maksim Kolchin, Karla Mendivelso, Andrea F. Lopez-Clavijo, Caroline Ready, Shankar Subramaniam, Valerie B. O'Donnell
Summary: LIPID MAPS is a systematic and standardized approach for organizing lipid structural and biochemical data, and it has become the accepted community standard. It provides databases, software tools, and educational resources. The recent expansion of LIPID MAPS includes richer metadata, improved interoperability, and programmatic access. In addition, LIPID MAPS collaborates with WikiPathways to curate pathway data and annotate lipids.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Pathology
Christina M. Mckenzie, Matt Marinkovich, Anibal G. Armien, Judy St. Leger, Aaron M. Armando, Edward A. Dennis, Oswald Quehenberger, Alison Righton
Summary: This study presents the pedigree analysis, clinical manifestations, gross, microscopic, ultrastructural, and lipidomic findings of four female superb bird-of-paradise (SBOP) siblings, revealing a primary inherited glycerolipid storage disease. These birds, offspring of closely related parents, displayed characteristic lesions including tissue distortion due to the accumulation of lipid vacuoles in various organs. Lipidomic profiling confirmed the presence of triacylglycerols in the cytoplasmic lipid deposits. Further investigations, such as genome sequencing and genotyping, are necessary to determine the underlying genetic mechanism of this disease.
VETERINARY PATHOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Varnavas D. Mouchlis, Edward A. Dennis
Summary: Water-soluble proteins and membrane-bound proteins bind specific lipid molecules on membrane surfaces. Phospholipases, especially PLA2, play an important role in hydrolyzing phospholipids. The interaction between PLA2 and membranes can induce conformational changes in PLA2 and activate it for catalysis. These studies provide insights into membrane-protein interactions and related biological functions.
ACCOUNTS OF CHEMICAL RESEARCH
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
E. A. Dennis