Review
Biochemistry & Molecular Biology
John C. Christianson, Pedro Carvalho
Summary: This article discusses recent advances in understanding the mechanisms of ERAD and its impact on the regulation of ER functions.
Article
Biochemistry & Molecular Biology
Ben P. Phillips, Elizabeth A. Miller
Summary: Membrane proteins account for a quarter of cellular proteins and are synthesized at the endoplasmic reticulum. Recent discoveries have advanced our understanding of protein insertion, quality control systems, and how cells resolve issues. Overall, a complex quality control network is emerging.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kohta Miura, Riko Katsuki, Shusei Yoshida, Ren Ohta, Taku Tamura
Summary: ERAD is an essential process for maintaining cell function and preventing ER stress. EDEM1 was found to be involved in accelerating the degradation of EGFR via ERAD, highlighting its physiological role in maintaining specific target proteins and providing a potential approach to regulate the expression of clinically important proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ola Jassar, Murad Ghanim
Summary: The carrot psyllid in Israel transmits CLso haplotype D in a persistent manner, causing significant economic losses. This study confirms the role of the endoplasmic reticulum (ER) in inducing immune responses and programmed cell death upon CLso infection in the insect gut.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Virology
Keisuke Tabata, Masashi Arakawa, Kotaro Ishida, Makiko Kobayashi, Atsuki Nara, Takehiro Sugimoto, Tetsuya Okada, Kazutoshi Mori, Eiji Morita
Summary: The study found that the ERAD system controls the amount of each viral protein in virus-infected cells, with viral protein homeostasis critical for viral propagation. The convoluted membrane (CM) serves as a waste dump for viral protein degradation, and the Derlin2/SEL1L/HRD1-specific pathway is involved in this process. This novel ERAD-mediated fine-tuning system for the stoichiometries of polyprotein-derived viral proteins may be a common feature among polyprotein-encoding viruses.
JOURNAL OF VIROLOGY
(2021)
Review
Plant Sciences
Sofia Reyes-Impellizzeri, Adrian A. Moreno
Summary: This review focuses on the impact of alterations in components of ERQC, ERAD, and UPR on plant responses to abiotic stresses. Evidence shows a clear connection between ERAD and UPR mechanisms, but the connection of ERQC components with these processes or their possible client proteins is lacking. Proteomics approaches are suggested to uncover the identity of these proteins and their connection to ER proteostasis.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Daria Sicari, Federica G. Centonze, Raphael Pineau, Pierre-Jean Le Reste, Luc Negroni, Sophie Chat, M. Aiman Mohtar, Daniel Thomas, Reynald Gillet, Ted Hupp, Eric Chevet, Aeid Igbaria
Summary: This study identified the accumulation of ER luminal proteins in the cytosol of glioblastoma cells, and demonstrated that ER protein reflux to the cytosol occurs upon ER proteostasis perturbation. The study also showed that refluxed, cytosolic AGR2 binds and inhibits the tumor suppressor p53, suggesting a mechanism named ER to Cytosol Signaling (ERCYS) which provides a selective advantage to tumor cells through gain-of-cytosolic functions.
Article
Environmental Sciences
Xiaoyu Wang, Fan Yang, Xiaomin Tian, Haihua Huo, Xinrun Li, Haitong Wu, Jianying Guo
Summary: Excessive copper can cause histopathological damage to duck cerebrums, disrupt the balance of trace elements, induce oxidative stress and activation of ER quality control, resulting in duck cerebrums damage.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Nicola A. Scott, Laura J. Sharpe, Andrew J. Brown
Summary: MARCHF6 is a large multi-pass E3 ubiquitin ligase embedded in the membranes of the endoplasmic reticulum, participating in endoplasmic reticulum associated degradation and being regulated by cholesterol. It plays a role in cell metabolism and its regulation, substrates, and role in human health and disease are still not fully understood.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Review
Biochemistry & Molecular Biology
Mari A. Piirainen, Alexander D. Frey
Summary: Yeasts are widely used for biopharmaceutical production, but N-glycosylated biopharmaceuticals manufactured with yeasts are missing on the market. N-linked glycans play crucial roles in protein properties and folding, but current glycoengineering approaches face challenges.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Kei Taguchi, Masayuki Kaneko, Serika Motoike, Kana Harada, Izumi Hide, Shigeru Tanaka, Norio Sakai
Summary: The ubiquitin E3 ligase HRD1 is demonstrated to be involved in the degradation of SERT protein, promoting SERT ubiquitination and affecting its uptake activity. This involvement in endoplasmic reticulum-associated degradation contributes to the membrane trafficking and functional regulation of SERT.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Cell Biology
Sally Badawi, Feda E. Mohamed, Divya Saro Varghese, Bassam R. Ali
Summary: Endoplasmic reticulum-associated protein degradation (ERAD) is a quality control mechanism that targets misfolded, unassembled, and native proteins for degradation. This review focuses on genetic variations in genes encoding ERAD components and their association with human single-gene disorders. Additionally, it discusses various genetically manipulated cellular and animal models lacking specific components of the ERAD pathway.
Article
Multidisciplinary Sciences
Adrian B. Mehrtash, Mark Hochstrasser
Summary: The study reveals that the conserved C-terminal element of Doa10 promotes E3-mediated Ubc6 activity, and substrates undergoing an alternative ubiquitylation mechanism are still degraded in its mutant cells. Structure predictions and intragenic suppression confirm the direct role of CTE in substrate ubiquitylation, while truncation analysis defines a minimal E2-binding region of Doa10.
Article
Biochemistry & Molecular Biology
Satarupa Bhaduri, Analine Aguayo, Yusuke Ohno, Marco Proietto, Jasmine Jung, Isabel Wang, Rachel Kandel, Narinderbir Singh, Ikran Ibrahim, Amit Fulzele, Eric J. Bennett, Akio Kihara, Sonya E. Neal
Summary: This study reveals the association between the pseudoprotease Dfm1 and the SPOTS complex, highlighting their roles in sphingolipid metabolism. Additionally, Dfm1 is found to play a crucial role in the ER export and degradation of Orm2. These findings provide insights into the molecular mechanisms underlying sphingolipid metabolism and its implications in disease.
Article
Biochemistry & Molecular Biology
Kei Miyano, Shuichiro Okamoto, Mizuho Kajikawa, Takuya Kiyohara, Chikage Kawai, Akira Yamauchi, Futoshi Kuribayashi
Summary: This study characterized missense mutations in p22(phoX) (L51Q, L52P, E53V, and P55R) that enhance the degradation of the protein in the endoplasmic reticulum (ER) and its binding to Derlin-1, a component of ER-associated degradation (ERAD). These mutations contribute to chronic granulomatous disease (CGD). The oxidation of Cys-50 appears to regulate the degradation of p22(phoX) and its interaction with Derlin-1.
Article
Cell Biology
Nicole E. Seah, C. Daniel de Magalhaes Filho, Anna P. Petrashen, Hope R. Henderson, Jade Laguer, Julissa Gonzalez, Andrew Dillin, Malene Hansen, Louis R. Lapierre
Review
Cell Biology
Louis R. Lapierre, Caroline Kumsta, Marco Sandri, Andrea Ballabio, Malene Hansen
Article
Biochemistry & Molecular Biology
Vanessa DeClercq, Brandon d'Eon, Roger S. McLeod
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2015)
Article
Nutrition & Dietetics
Genevieve Chevrier, Patricia L. Mitchell, Laurie-Eve Rioux, Fida Hasan, Tianyi Jin, Cyril Roland Roblet, Alain Doyen, Genevieve Pilon, Philippe St-Pierre, Charles Lavigne, Laurent Bazinet, Helene Jacques, Tom Gill, Roger S. McLeod, Andre Marette
JOURNAL OF NUTRITION
(2015)
Article
Multidisciplinary Sciences
C. Daniel De Magalhaes Filho, Brian Henriquez, Nicole E. Seah, Ronald M. Evans, Louis R. Lapierre, Andrew Dillin
NATURE COMMUNICATIONS
(2018)
Article
Cell Biology
Melissa J. Silvestrini, Joseph R. Johnson, Anita V. Kumar, Tara G. Thakurta, Karine Blais, Zachary A. Neill, Sarah W. Marion, Victoria St Amand, Robert A. Reenan, Louis R. Lapierre
Article
Cell Biology
Louis R. Lapierre, Melissa J. Silvestrini, Lizbeth Nunez, Kristina Ames, Sara Wong, Thuc T. Le, Malene Hansen, Alicia Melendez
Article
Multidisciplinary Sciences
Louis R. Lapierre, C. Daniel De Magalhaes Filho, Philip R. McQuary, Chu-Chiao Chu, Orane Visvikis, Jessica T. Chang, Sara Gelino, Binnan Ong, Andrew E. Davis, Javier E. Irazoqui, Andrew Dillin, Malene Hansen
NATURE COMMUNICATIONS
(2013)
Review
Genetics & Heredity
Shi Q. Wong, Anita V. Kumar, Joslyn Mills, Louis R. Lapierre
Review
Biophysics
Anita V. Kumar, Louis R. Lapierre
Summary: This review article summarizes recent literature on the nucleocytoplasmic partitioning of proteins, quality control mechanisms in the nucleus and cytoplasm, and their impact on age-related diseases. It also highlights new approaches to modulate spatially-regulated proteostatic mechanisms to delay aging.
BIOPHYSICAL REVIEWS
(2021)
Article
Multidisciplinary Sciences
Anita Kumar, Taewook Kang, Tara G. Thakurta, Celeste Ng, Aric N. Rogers, Martin R. Larsen, Louis R. Lapierre
Summary: Altered nucleolar and ribosomal dynamics are key hallmarks of aging, and their regulation involves the nuclear export receptor XPO-1 and the autophagy protein LGG-1. XPO-1 modulates the distribution of key proteins involved in nucleolar dynamics and ribosome function, while LGG-1 regulates nucleolar size by controlling protein degradation and ribosome metabolism.
Article
Cell Biology
Shi Quan Wong, Catherine J. Ryan, Dennis M. Bonal, Joslyn Mills, Louis R. Lapierre
Summary: Neuronal TFEB plays an important role in stress resistance and longevity regulation. Its rescue improves heat stress resistance in wildtype animals but not daf-2 mutants. Neuronal TFEB modulates neurotransmission through the uncharacterized protein W06A11.1, inducing peripheral mitochondrial fragmentation and enhancing organismal heat stress resistance.
Article
Geriatrics & Gerontology
Lucas Paulo de Lima Camillo, Louis R. Lapierre, Ritambhara Singh
Summary: This study explores the improvement and interpretability of epigenetic clocks using deep learning. A neural network framework called AltumAge is developed, which performs better in age prediction and provides more accurate model interpretations. The study also identifies important CpG sites related to gene regulatory regions and analyzes the age acceleration in various diseases using AltumAge.
Review
Cell Biology
Hong Zhang, Jessica T. Chang, Bin Guo, Malene Hansen, Kailiang Jia, Attila L. Kovacs, Caroline Kumsta, Louis R. Lapierre, Renaud Legouis, Long Lin, Qun Lu, Alicia Melendez, Eyleen J. O'Rourke, Ken Sato, Miyuki Sato, Xiaochen Wang, Fan Wu
Review
Medicine, Research & Experimental
Eric Fisher, Elizabeth Lake, Roger S. McLeod
JOURNAL OF BIOMEDICAL RESEARCH
(2014)