Review
Immunology
Ye Zheng, Xiaoyu Ma, Shouchang Feng, Hongtao Zhu, Xin Chen, Xingjiang Yu, Kai Shu, Suojun Zhang
Summary: Glioblastoma (GBM), the most malignant subtype of glioma, is resistant to traditional therapies and immunotherapies. Dendritic cell vaccine (DCV) has shown potential as a therapeutic method, but there are challenges to its use.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Immunology
Xi Zhang, Tianhui He, Yuan Li, Ling Chen, Hongyu Liu, Yu Wu, Hongyan Guo
Summary: Ovarian cancer is characterized by uncertain presentation and poor outcomes, with surgery and chemotherapy being the current basis of treatment. However, there are limitations due to advanced stage at diagnosis and high recurrence rate. The use of anti-VEGF agents, PARP inhibitors, and immunotherapies are being explored to enhance treatment outcomes, but the population that can benefit from these treatments remains limited.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Heng Dong, Qiang Li, Yu Zhang, Meng Ding, Zhaogang Teng, Yongbin Mou
Summary: Dendritic cell (DC)-based cancer immunotherapy has shown great potential in clinical application. However, traditional DC-mediated immunotherapy faces challenges such as insufficient antigen delivery, inadequate antigen presentation, and high levels of immunosuppression. To overcome these challenges, engineered biomaterials have been utilized to enhance the effects of DC-mediated immunotherapy. This review summarizes the key components that can enhance DC-mediated immunotherapeutic effects, discusses the parameters considered in the design of biomaterials, and reviews recent applications of engineered biomaterials in DC-mediated immunotherapy, including as delivery platforms and modulators of the tumor microenvironment. The review aims to inspire the clinical translation of future DC-mediated cancer immunotherapies.
Review
Immunology
Eleonora Russo, Mattia Laffranchi, Luana Tomaipitinca, Annalisa Del Prete, Angela Santoni, Silvano Sozzani, Giovanni Bernardini
Summary: NK cells, as innate lymphoid cells, play key roles in immune surveillance of tumors with cytotoxic capacity. Increasing evidence shows that NK cell anti-tumor response is influenced by bidirectional interactions with myeloid cell subsets such as dendritic cells and macrophages in the tumor microenvironment. This crosstalk can impact NK cell survival, activation, differentiation, and function, ultimately affecting the efficacy of immunotherapeutic approaches for cancer.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
M. Hope Robinson, Nancy Y. Villa, David L. Jaye, Ajay K. Nooka, Alyssa Duffy, Samuel S. McCachren, Julia Manalo, Jeffrey M. Switchenko, Sierra Barnes, Sayalee Potdar, Maryam I. Azeem, Ava A. Horvat, Vaunita C. Parihar, Jingjing Gong, Yan Liang, Geoffrey H. Smith, Vikas A. Gupta, Lawrence H. Boise, Jonathan L. Kaufman, Craig C. Hofmeister, Nisha S. Joseph, Sagar Lonial, Kavita M. Dhodapkar, Madhav V. Dhodapkar
Summary: This study investigates the mechanisms underlying immune infiltration in human multiple myeloma (MM) and its precursor monoclonal gammopathy of undetermined significance (MGUS) using high-dimensional spatial analyses and in vitro and in vivo modeling. The results suggest that MM exhibits clustered tumor growth and spatial heterogeneity with the coexistence of T cell-rich and T cell-sparse regions, as well as areas of T cell exclusion. T cell entry into MM clusters is regulated by agonistic signals and CD2-CD58 interactions, and CLEC9A+ DCs play a role in marking the portals of entry for T cell infiltration.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Review
Oncology
Jing Zhou, Luohong Li, Minqi Jia, Qianjin Liao, Guiping Peng, Gengqiu Luo, Yanhong Zhou
Summary: This paper discusses the reasons for the dysfunction of DCs in the tumor microenvironment, analyzes the impact of DC vaccines on improving the immunosuppressive microenvironment in glioma patients, and proposes new development directions and challenges for DC vaccines.
Article
Cell Biology
Remy Vu, Suoqin Jin, Peng Sun, Daniel Haensel, Quy Hoa Nguyen, Morgan Dragan, Kai Kessenbrock, Qing Nie, Xing Dai
Summary: This study used single-cell RNA sequencing to analyze the cellular and communication changes in young and aged skin wounds. It found that aged skin wounds have a more pronounced inflammatory response and higher abundance of inflammatory/glycolytic macrophages. The study also predicted differences in cell-cell communication between young and aged skin wounds.
Review
Oncology
Qingyu Huang, Fuhao Wang, Di Hao, Xinyu Li, Xiaohui Li, Tianyu Lei, Jinbo Yue, Chao Liu
Summary: Dendritic cells play a pivotal role in connecting innate and adaptive immunity. The application of single-cell sequencing has revealed the heterogeneity and functional diversity of dendritic cells in the tumor microenvironment, providing important insights into tumor immune responses.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2023)
Article
Immunology
Yu-Chen Wang, Yang Cao, Calvin Pan, Zhiqiang Zhou, Lili Yang, Aldons J. Lusis
Summary: This study utilized single-cell RNA sequencing and statistical modeling to investigate the dynamic properties and underlying cellular mechanisms of intestinal cells. It identified new cell subsets and developmental trajectories in different layers of intestinal cells. The high-fat high-sucrose Western diet led to changes in immune cell populations and nutrient absorption function in enterocytes, as well as revealed novel interactions and communication hubs among intestinal cells.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Zhou Chen, Zhengfeng Wang, Yan Du, Huaqing Shi, Wence Zhou
Summary: Cellular senescence plays a dual role in tissue development and tumor suppression, but can also contribute to age-related diseases. The microbiota is intricately linked to cellular senescence and may play a significant role in tumorigenesis and progression.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2022)
Article
Pharmacology & Pharmacy
Yun Chen, Boyuan Liu, Yuan Wei, Dong-Ming Kuang
Summary: The impact of microbiota on disease progression varies depending on the tumor types, therapeutic regimens, and microbiota composition, highlighting the need for a deeper understanding of host-microbiome interactions. Gut microbiota influences disease progression by regulating local and systemic immunity, with intratumoral microbiota being an important component of the tumor microenvironment. The identification of intra-tumor microbiota and its role in immune microenvironment interaction may benefit current anti-cancer approaches.
PHARMACOLOGICAL RESEARCH
(2021)
Review
Immunology
Ioana Plesca, Luise Mueller, Jan P. Boettcher, Hind Medyouf, Rebekka Wehner, Marc Schmitz
Summary: Dendritic cells (DCs) are crucial in orchestrating anti-tumor immunity, capable of producing proinflammatory cytokines, initiating T-cell responses, and exhibiting direct cytotoxicity against tumor cells. The functionality of DCs depends on intrinsic properties and the tumor microenvironment, with immunogenic DCs promoting tumor elimination and tolerogenic DCs fostering tumor progression.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Biology
Yuexin Wang, Hui Yang, Anna Jia, Yufei Wang, Qiuli Yang, Yingjie Dong, Yueru Hou, Yejin Cao, Lin Dong, Yujing Bi, Guangwei Liu
Summary: Dendritic cells (DCs) are important in anti-tumor immunity, and the mechanical sensor Piezo1 plays a role in regulating the differentiation of T(H)1 and T-reg cells in response to mechanical stiffness or inflammatory signals. Genetic deletion of Piezo1 in DCs inhibits T(H)1 cell generation and promotes T-reg cell development, leading to cancer growth. Mechanistically, Piezo1-deficient DCs regulate the secretion of cytokines TGF beta 1 and IL-12, affecting the activity of TGF beta R2-p-Smad3 and IL-12R beta 2-p-STAT4, and influencing the differentiation of T-reg and T(H)1 cells. Additionally, Piezo1 integrates metabolic and signaling pathways to orchestrate T(H)1 and T-reg lineage commitment through IL-12 and TGF beta 1 derived from DCs. Our studies provide critical insight into the role of DC-based mechanical regulation in directing T cell lineage commitment in tumor microenvironments.
Article
Immunology
Roque Pastor-Ibanez, Francisco Diez-Fuertes, Sonsoles Sanchez-Palomino, Jose Alcami, Montserrat Plana, David Torrents, Lorna Leal, Felipe Garcia
Summary: The study aimed to investigate the mRNA expression profiles and gut microbiome composition in predicting viral load control after antiretroviral therapy interruption. It was found that therapeutic vaccines based on dendritic cells may play a role in controlling viral load among HIV-infected individuals. Additionally, responders had enriched levels of specific bacteria in the gut microbiota associated with short-chain fatty acid production, which is important for intestinal homeostasis regulation.
Article
Oncology
Ran You, Jordan Artichoker, Arja Ray, Hugo Gonzalez Velozo, Dan A. Rock, Kip P. Conner, Matthew F. Krummel
Summary: This study characterized the exposure and pharmacodynamic response of an anti-muCD3/anti-huEGFRvIII mouse surrogate BiTE molecule in EGFR variant III-positive breast tumors using intravital imaging. The results revealed heterogeneous temporal and spatial dynamics of BiTE molecule extravasation into solid tumors, indicating physical barriers to its function. High and homogeneous EGFRvIII expression on cancer cells was found to be necessary for efficient tumor clearance by the BiTE molecule. Additionally, the optimal tumor killing required resident tumor-infiltrating lymphocytes (TIL) at high BiTE molecule dosage, while inclusion of peripheral T-cell recruitment was synergistic at moderate to low dosages. Deletion of stimulating conventional type I dendritic cells (cDC1) diminished T-cell activation and tumor clearance induced by the BiTE molecule, suggesting the modulation of in situ antigen-presenting cell (APC) engagements on its efficacy.
CANCER IMMUNOLOGY RESEARCH
(2022)
Article
Multidisciplinary Sciences
Wei Hu, Aakanksha Jain, Yajing Gao, Igor M. Dozmorov, Rajakumar Mandraju, Edward K. Wakeland, Chandrashekhar Pasare
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Immunology
Mohammed Ali Akbar, Rajakumar Mandraju, Charles Tracy, Wei Hu, Chandrashekhar Pasare, Helmut Kramer
Article
Immunology
Wei Hu, Ty Dale Troutman, Ramakrishna Edukulla, Chandrashekhar Pasare
Article
Medicine, Research & Experimental
Scott N. Furlan, Rajakumar Mandraju, Travis Brewer, Kole Roybal, Ty Dale Troutman, Wei Hu, Noah W. Palm, Arun Unni, Chandrashekhar Pasare
Article
Multidisciplinary Sciences
Ty Dale Troutman, Wei Hu, Stephanie Fulenchek, Tetsuo Yamazaki, Tomohiro Kurosaki, J. Fernando Bazan, Chandrashekhar Pasare
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2012)
Article
Multidisciplinary Sciences
Keng-Mean Lin, Wei Hu, Ty Dale Troutman, Michelle Jennings, Travis Brewer, Xiaoxia Li, Sambit Nanda, Philip Cohen, James A. Thomas, Chandrashekhar Pasare
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Immunology
Wei Hu, Zhong-Min Wang, Yongqiang Feng, Michail Schizas, Beatrice E. Hoyos, Joris van der Veeken, Jacob G. Verter, Regina Bou-Puerto, Alexander Y. Rudensky
Summary: Regulatory T cells play a crucial role in preventing fatal autoimmune inflammation, exerting their function in settings of established broad-spectrum systemic inflammation, and enabling sustained reset of immune homeostasis.
Article
Multidisciplinary Sciences
Johanne T. Jacobsen, Wei Hu, Tiago B. R. Castro, Sigrid Solem, Alice Galante, Zeran Lin, Samuel J. Allon, Luka Mesin, Angelina M. Bilate, Arien Schiepers, Alex K. Shalek, Alexander Y. Rudensky, Gabriel D. Victora
Summary: Research shows that contraction of immunization-induced germinal centers is associated with an acute surge in GC-resident Foxp3(+) T cells, attributed to the up-regulation of Foxp3 by T follicular helper cells. This natural up-regulation of Foxp3 in T-FH cells may play a role in regulating GC lifetimes.
Article
Immunology
Joris van der Veeken, Clarissa Campbell, Yuri Pritykin, Michail Schizas, Jacob Verter, Wei Hu, Zhong-Min Wang, Fanny Matheis, Daniel Mucida, Louis-Marie Charbonnier, Talal A. Chatila, Alexander Y. Rudensky
Summary: The role of Foxp3 in peripheral pTreg cells and the mechanisms supporting their differentiation remain poorly understood. This study used genetic tracing to identify microbiota-induced pTreg cells and found that many of their distinguishing features were Foxp3 independent. While Foxp3 was critical for the suppression of certain diseases, pTreg cells could suppress colonic effector T cell expansion in a Foxp3-independent manner. Thus, Foxp3 and the tolerogenic signals preceding and promoting its expression confer distinct facets of pTreg functionality.
Article
Immunology
Ariella Glasner, Samuel A. Rose, Roshan Sharma, Herman Gudjonson, Tinyi Chu, Jesse A. Green, Sham Rampersaud, Izabella K. Valdez, Emma S. Andretta, Bahawar S. Dhillon, Michail Schizas, Stanislav Dikiy, Alejandra Mendoza, Wei Hu, Zhong-Min Wang, Ojasvi Chaudhary, Tianhao Xu, Linas Mazutis, Gabrielle Rizzuto, Alvaro Quintanal-Villalonga, Parvathy Manoj, Elisa de Stanchina, Charles M. Rudin, Dana Pe'er, Alexander Y. Rudensky
Summary: Traditional roles of regulatory T (T-reg) cells as suppressors of antigen presenting cells and effector T cells have expanded to include tissue maintenance functions, suggesting a broader regulatory role than previously thought. In lung cancer and injury-induced inflammation, T-reg cell depletion led to changes in gene expression in fibroblasts, endothelial and myeloid cells, involving VEGF and CCR2 signaling. Combined T-reg cell depletion and short-term VEGF blockade showed improved control of PD-1 blockade-resistant lung adenocarcinoma progression, highlighting the potential of combination therapies for solid organ cancers.
Article
Immunology
Rahul Sharma, Ryan M. Smolkin, Priyanka Chowdhury, Keith Conrad Fernandez, Youngjun Kim, Montserrat Cols, William Alread, Wei-Feng Yen, Wei Hu, Zhong-Min Wang, Sara Violante, Ronan Chaligne, Ming O. Li, Justin R. Cross, Jayanta Chaudhuri
Summary: Sharma et al. identify an early metabolic checkpoint dependent upon lactate dehydrogenase for newly activated B cells to enter germinal centers. The metabolic requirements of naive and activated B cells differ and are regulated by niche and cellular interactions.
Article
Immunology
Joris van der Veeken, Ariella Glasner, Yi Zhong, Wei Hu, Zhong-Min Wang, Regina Bou-Puerto, Louis-Marie Charbonnier, Talal A. Chatila, Christina S. Leslie, Alexander Y. Rudensky
Article
Biochemistry & Molecular Biology
Fan Yang, Wei Hu, Huimin Xu, Congmin Li, Bin Xia, Changwen Jin
JOURNAL OF BIOLOGICAL CHEMISTRY
(2007)
Article
Biochemistry & Molecular Biology
Ewen Lescop, Yunfei Hu, Huimin Xu, Wei Hu, Juan Chen, Bin Xia, Changwen Jin
JOURNAL OF BIOLOGICAL CHEMISTRY
(2006)