期刊
JOURNAL OF LEUKOCYTE BIOLOGY
卷 84, 期 6, 页码 1557-1564出版社
WILEY
DOI: 10.1189/jlb.0706457
关键词
B-cell antigen receptor; CD40; signal transduction; transitional B cells
资金
- Korea Health 21 RD Project [02-PJ1-PG3-212020004)]
- Ministry of Health and Welfare
- Korea Research Foundation [R04-2004-000-10077-0]
- Korea Science and Engineering Foundation (KOSEF) [R01-2006-00010429-0]
- Ministry of Education Science, and Technology [R15-2006-020]
Human cord blood (CB) is a valuable source of hematopoietic stem cells, but clinical reports have indicated slow recovery of B-cell development and function after CB transplantation. To investigate the basis of these B-cell defects in reconstitution, we characterized B cells purified from CB. We compared B-cell receptor activation and B-cell subsets in CB, bone marrow (BM), and peripheral blood (PB). We found that in CB B cells activation of extracellular signal-regulated kinase (ERK) and p38 following ligation of CD40 but not of the B-cell antigen receptor (BCR) was inefficient. The patterns of expression of CD5, CD34, and CD40 in the B-cell population of CB were similar to those in PB rather than in BM. The B cells in CB contained an increased proportion of B cells expressing a high level of CD24 and a low proportion of B cells expressing CD27, pointing to the presence of circulating CD24(high) immature transitional and CD27(-) naive B cells. CD40-mediated activation of ERK and p38 was also minimal in these B cells of CB. These findings may account for the functional defects of B cells in transplanted CB. J. Leukoc. Biol. 84: 1557-1564; 2008.
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