Article
Oncology
Giovanni Nicolao Berta, Federica Di Scipio, Zhiqian Yang, Alessandra Oberto, Giuliana Abbadessa, Federica Romano, Maria Elisabetta Carere, Adriano Ceccarelli, Emilio Hirsch, Barbara Mognetti
Summary: The study revealed that the lack or inhibition of PI3K gamma can delay preneoplastic and exophytic lesions in oral carcinogenesis. WT mice showed higher expression of prognostic markers and certain cell types, while tongues isolated from transgenic mice had more abundant T lymphocytes. A specific PI3K gamma inhibitor significantly impaired the vitality of oral cancer cells.
Article
Physiology
Lin Zhu, Julia Duerr, Zhe Zhou-Suckow, Willi Wagner, Oliver Weinheimer, Johanna Salomon, Dominik Leitz, Philip Konietzke, Hong Yu, Maximilian Ackermann, Wolfram Stiller, Hans-Ulrich Kauczor, Marcus A. Mall, Mark O. Wielpuetz
Summary: This study evaluated the use of mu CT for longitudinal quantification of muco-obstructive lung disease in beta-epithelial Na+ channel overexpressing mice and the effects of neutrophil elastase knockout on its progression. The results demonstrate that mu CT is sensitive to detect the onset and progression of muco-obstructive lung disease and the effects of genetic deletion of NE on airways and lung parenchyma morphology in these mice.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Na Liu, Yanhong Si, Ying Zhang, Shoudong Guo, Shucun Qin
Summary: The study demonstrates that CETP-tg improves RCT efficiency in mice, with a similar effect observed in PLTP KO mice. CETP-tg primarily enhances RCT by facilitating the transport of [H-3]-cholesterol and reducing oxidized lipid levels.
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Immunology
MeiNa Dai, XinHua Zhu, Juan Yu, JiaSheng Yuan, Yv Zhu, YouWei Bao, XiaoZhuang Yong
Summary: The present study investigated the effects of CCR3 gene knockout in a mouse model of combined allergic rhinitis and asthma syndrome (CARAS). The results showed that CCR3 knockout reduced allergic inflammation symptoms and down-regulated the expression of related inflammatory markers. This suggests that CCR3 gene could be a potential target for the treatment of CARAS.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiaolan Guo, Haobin Li, Xiuqiong Meng, ZhiBin Zhao, Rongxin Zhang, Lijing Wang, Jiangchao Li
Summary: CD8 + effector cells play a crucial role in immune surveillance and adaptive immunity against cancer cells. Knockout of the Shkbp1 gene increases the number and enhances the function of CD8 + T cells in the spleen. This study suggests that Shkbp1 may be a potential target in cancer immunotherapy.
MOLECULAR IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Bao Ngoc Tran, Lucie Valek, Annett Wilken-Schmitz, Dominik Christian Fuhrmann, Dimitry Namgaladze, Ilka Wittig, Irmgard Tegeder
Summary: The study showed that Nucleoredoxin plays a crucial role in neuronal function, impacting neuronal plasticity and synaptic protein activity. Mice deficient in synaptic protein Nestin-NXN-/- displayed normal behavior in tests, but showed reduced interest in rewards.
Article
Cell Biology
Bastian Brauer, Nicolas Merino-Veliz, Constanza Ahumada-Marchant, Gloria Arriagada, Fernando J. Bustos
Summary: Using CRISPR/Cas9 gene editing, researchers found that knocking out KMT2C expression in cortical neurons and the mouse brain led to decreased KMT2C levels and the development of repetitive behaviors, social deficits, and intellectual disability resembling ASD. This study sheds light on the importance of KMT2C in neurodevelopmental processes and establishes a valuable model for understanding the cellular and molecular mechanisms underlying KMT2C mutations and their relationship to Kleefstra syndrome 2 and ASD.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Xiaodong Jiao, Zhiwei Ma, Jingqi Lei, Pinghu Liu, Xiaoyu Cai, Pawan K. Shahi, Chi-Chao Chan, Robert Fariss, Bikash R. Pattnaik, Lijin Dong, J. Fielding Hejtmancik
Summary: By constructing knockout and conditional knockout mice for KCNJ13, this study provides insights into the pathology of Snowflake Vitreoretinal Degeneration (SVD) and Retinitis pigmentosa (RP), and offers a potential model system for gene therapy trials.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Hisashi Goto, Takeshi Kikuchi, Yuhei Takayanagi, Yosuke Kamiya, Yuki Suzuki, Shotaro Kawamura, Noritaka Sawada, Jun-ichiro Hayashi, Akio Mitani
Summary: This study aimed to investigate the role of Ebi3-related cytokines in experimental periodontitis using Ebi3 knockout mice. The results showed that Ebi3 KO mice exhibited more severe alveolar bone resorption and higher levels of IL-17A expression, while IL-10 expression was lower. Injection of recombinant IL-35 reduced ligature-induced alveolar bone resorption in Ebi3 KO mice. These findings suggest that Th17 cells exacerbate experimental periodontitis in mice lacking Ebi3, and IL-35 may play a critical role in inhibiting periodontal tissue destruction.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2023)
Article
Oncology
Xiaoyun Tang, Andrew J. Morris, Marcel A. Deken, David N. Brindley
Summary: Breast cancer cells do not produce much autotaxin, but adipocytes in inflamed adipose tissue near breast tumors are a major source of autotaxin secretion that promotes tumor growth, metastasis, and resistance to chemotherapy and radiotherapy. Knocking out autotaxin in adipocytes does not decrease tumor growth in mice with breast tumors, but inhibiting autotaxin with IOA-289 does. Tumor-associated fibroblasts and leukocytes are the main sources of autotaxin that drive breast tumor growth.
Article
Cell Biology
Waka Ishida, Tatsuma Kishimoto, Fumio Takaiwa, Ken Fukuda
Summary: Oral administration of transgenic rice seeds expressing a hypoallergenic Bet v 1 derivative showed prophylactic and therapeutic effects in reducing allergic birch pollen conjunctivitis in mice. The treatment resulted in improved clinical scores, reduced eosinophil numbers, and modulation of IL-4 and IFN-gamma production.
Article
Immunology
Brigitta Dukay, Fruzsina R. Walter, Judit P. Vigh, Beata Barabasi, Petra Hajdu, Tamas Balassa, Ede Migh, Andras Kincses, Zsofia Hoyk, Titanilla Szogi, Emoke Borbely, Balint Csoboz, Peter Horvath, Livia Fulop, Botond Penke, Laszlo Vigh, Maria A. Deli, Miklos Santha, Melinda E. Toth
Summary: The study revealed that overexpression of HSPB1 can enhance the expression of inflammatory cytokines and glial cell activation in neuroinflammatory processes, without increasing neuronal apoptosis. The results suggest that HSPB1 may play a complex role in modulating ethanol-induced neuroinflammatory responses.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Biochemistry & Molecular Biology
Laeticia Scherler, Sofia N. Verouti, Daniel Ackermann, Bruno Vogt, Genevieve Escher
Summary: In this study, adenine was used to induce nephropathy in mice, showing the loss of protective effects of renal cholesterol and vitamin D-3 metabolism under this condition. It was found that adenine enhanced cholesterol efflux and eliminated lipids in feces, which had a protective effect against atherosclerosis but also affected bone growth.
Review
Biochemistry & Molecular Biology
Ryosuke Saiki, Kan Katayama, Kaoru Dohi
Summary: Proteinuria is associated with mortality, and nephrotic syndrome is defined by proteinuria and hypoalbuminemia. Genetic kidney diseases have been discovered more frequently with advances in medicine. This review focuses on the study of proteinuria/nephrotic syndrome and other kidney diseases in mouse models, leading to significant progress in understanding genetics and pathophysiology. Future genetic analysis of proteinuric kidney disease/nephrotic syndrome may provide personalized treatment options.
Article
Biochemistry & Molecular Biology
Jing Li, Xuewen Tang, Xing Wen, Xiaoyuan Ren, Huihui Zhang, Yatao Du, Jun Lu
Summary: Acetaminophen overdose is a common cause of drug-induced liver injury. The reaction between APAP metabolite and thiol molecules is the main cause of APAP-induced hepatotoxicity, but the role of other thiol-related regulators is unclear. In this study, the deletion of the Glrx2 gene worsened APAP-induced liver damage by interrupting the thiol-redox compensatory response and enhancing the AIF pathway-mediated oxidative damage.
Article
Immunology
Judith Agudo, Eun Sook Park, Samuel A. Rose, Eziwoma Alibo, Robert Sweeney, Maxime Dhainaut, Koichi S. Kobayashi, Ravi Sachidanandam, Alessia Baccarini, Miriam Merad, Brian D. Brown
Article
Multidisciplinary Sciences
Morris Nechama, Jeahoo Kwon, Shuo Wei, Adrian Tun Kyi, Robert S. Welner, Iddo Z. Ben-Dov, Mohamed S. Arredouani, John M. Asara, Chun-Hau Chen, Cheng-Yu Tsai, Kyle F. Nelson, Koichi S. Kobayashi, Elliot Israel, Xiao Zhen Zhou, Linda K. Nicholson, Kun Ping Lu
NATURE COMMUNICATIONS
(2018)
Review
Genetics & Heredity
Saptha Vijayan, Tabasum Sidiq, Suhail Yousuf, Peter J. van den Elsen, Koichi S. Kobayashi
Article
Food Science & Technology
Tingting Chen, Anna B. Liu, Shili Sun, Nadim J. Ajami, Matthew C. Ross, Hong Wang, Le Zhang, Kenneth Reuhl, Koichi Kobayashi, Janet C. Onishi, Liping Zhao, Chung S. Yang
MOLECULAR NUTRITION & FOOD RESEARCH
(2019)
Review
Immunology
Steven X. Cho, Saptha Vijayan, Ji-Seung Yoo, Toshiyuki Watanabe, Ryota Ouda, Ning An, Koichi S. Kobayashi
Summary: The presentation of antigenic peptides by MHC class I and class II molecules is crucial for activating the adaptive immune system, with CIITA and NLRC5 serving as the major transcriptional activators of MHC class II and class I gene expression. Recent advances in understanding the role of NLRC5 in infectious diseases and cancer have shed light on its biological significance and mechanism in MHC class I expression.
Article
Immunology
Mohamed Benhammadi, Justine Mathe, Maude Dumont-Lagace, Koichi S. Kobayashi, Louis Gaboury, Sylvie Brochu, Claude Perreault
JOURNAL OF IMMUNOLOGY
(2020)
Article
Multidisciplinary Sciences
Sayuri Yoshihama, Steven X. Cho, Jason Yeung, Xuedong Pan, Gregory Lizee, Kranti Konganti, Valen E. Johnson, Koichi S. Kobayashi
Summary: The expression of NLRC5 and MHC class I-related genes is crucial for the effective response of melanoma patients to anti-CTLA-4 and anti-PD1 checkpoint blockade immunotherapy, serving as potential predictive biomarkers for prognosis and treatment response.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Justine Mathe, Mohamed Benhammadi, Koichi S. Kobayashi, Sylvie Brochu, Claude Perreault
Summary: Lung infections are a major cause of death worldwide. Inhalation of the TLR4 ligand LPS is found to significantly upregulate MHC I expression on mouse lung epithelial cells. This upregulation is dependent on the production of three IFN families but limited in AT1 cells due to gene expression restrictions. Genes involved in cytokine and chemokine production are selectively induced in AT1 cells, while specific gene subsets are downregulated in AT2 or bronchiolar cells.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Ji-Seung Yoo, Michihito Sasaki, Steven X. Cho, Yusuke Kasuga, Baohui Zhu, Ryota Ouda, Yasuko Orba, Paul de Figueiredo, Hirofumi Sawa, Koichi S. Kobayashi
Summary: The authors show that SARS-CoV-2 targets key transcriptional regulators of the MHC class I pathway to hinder antigen presentation, revealing an immune evasion mechanism that suppresses host immune response.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Ying-Shiuan Chen, Jia Li, Sultan Neja, Sabeeta Kapoor, Jorge Enrique Tovar Perez, Chakrapani Tripathi, Rani Menon, Arul Jayaraman, Kyongbum Lee, Wan Mohaiza Dashwood, Shan Wang, Ke Zhang, Koichi Kobayashi, Praveen Rajendran, Roderick Dashwood
Summary: This study reveals the link between spinach-derived linoleate and butanoate metabolites and anticancer effects in a genetic model of colorectal cancer. Mechanistic studies demonstrate that these metabolites target HDAC activity and the IFN-gamma signaling axis. The clinical translation of these findings could have significant implications for delaying surgical interventions and drug therapies in at-risk patients.
Article
Multidisciplinary Sciences
Yusuke Kasuga, Ryota Ouda, Masashi Watanabe, Xin Sun, Miki Kimura, Shigetsugu Hatakeyama, Koichi S. Kobayashi
Summary: Major histocompatibility complex (MHC) class I and II molecules are critical for adaptive immunity. The protein FBXO11 has been identified as an E3 ligase that regulates CIITA protein level through ubiquitination-mediated degradation, thus determining the level of MHC-II.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Editorial Material
Pharmacology & Pharmacy
Baohui Zhu, Ryota Ouda, Paul de Figueiredo, Koichi S. Kobayashi
EXPERT OPINION ON THERAPEUTIC TARGETS
(2023)
Article
Oncology
Fengguang Guo, Jugal K. Das, Koichi S. Kobayashi, Qing-Ming Qin, Thomas A. Ficht, Robert C. Alaniz, Jianxun Song, Paul De Figueiredo
Summary: This study demonstrates that live attenuated bacterial treatment can improve antitumor immunity by remodeling the tumor microenvironment, overcoming cancer resistance to chimeric antigen receptor T-cell therapy.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Review
Oncology
Sayuri Yoshihama, Saptha Vijayan, Tabasum Sidiq, Koichi S. Kobayashi
Article
Microbiology
Aseem Pandey, Sheng Li Ding, Qing-Ming Qin, Rahul Gupta, Gabriel Gomez, Furong Lin, Xuehuan Feng, Luciana Fachini da Costa, Sankar P. Chaki, Madhu Katepalli, Elizabeth D. Case, Erin J. van Schaik, Tabasum Sidiq, Omar Khalaf, Angela Arenas, Koichi S. Kobayashi, James E. Samuel, Gonzalo M. Rivera, Robert C. Alaniz, Sing-Hoi Sze, Xiaoning Qian, William J. Brown, Allison Rice-Ficht, William K. Russell, Thomas A. Ficht, Paul de Figueiredo
CELL HOST & MICROBE
(2017)