Article
Dermatology
Mika Fujikawa, Hiroko Sugimoto, Rie Tamura, Koki Fujikawa, Ami Yamagishi, Yuhki Ueda
Summary: The moisturizing agent MPS can repair TJ barrier dysfunction and improve the reduction caused by histamine in AD patients, suggesting it as a new therapeutic option.
EXPERIMENTAL DERMATOLOGY
(2022)
Article
Cell Biology
Shilpashree Mallesh, Anne S. Ten Hove, Reiner Schneider, Bianca Schneiker, Patrik Efferz, Jorg C. Kalff, Wouter J. de Jonge, Sven Wehner
Summary: Intestinal mucosal cells express adrenergic receptors and are receptive to the neurotransmitter norepinephrine. This study investigated the effect of the sympathetic nervous system (SNS) on mucosal immune and epithelial cell functions. The results suggest that a loss in sympathetic input induces a proinflammatory mucosal state, leading to reduced barrier functioning and enhanced antimicrobial defense.
Article
Veterinary Sciences
Matteo Cuccato, Frine Eleonora Scaglione, Cinzia Centelleghe, Sara Divari, Bartolomeo Biolatti, Paola Pregel, Francesca Tiziana Cannizzo
Summary: This study investigates the role of different antimicrobial prophylaxis protocols in the modulation of gut barrier in broilers. Results show that antimicrobial treatments may harm the gut health of broilers, inducing an inflammatory response and epithelial lesions. However, the expression of gut barrier proteins was not affected by different antimicrobial treatments. Further research is needed to clarify the role of antimicrobial treatments in the modulation of gut barrier in broilers.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Atul K. Srivastava, Bharat Somireddy Venkata, Yan Y. Sweat, Heather R. Rizzo, Lea Jean-Francois, Li Zuo, Kathleen W. Kurgan, Patrick Moore, Nitesh Shashikanth, Izabela Smok, Joseph R. Sachleben, Jerrold R. Turner, Stephen C. Meredith
Summary: This study investigates the effects of S408 phosphorylation on occludin protein structure and demonstrates its role in regulating intercellular junction function through interactions with other components.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Min Liu, Yongwu Yang, Weining Zhu, Jingxue Wu, Xiran Yu, Wei Li
Summary: HSP70 plays an important role in regulating the anti-Giardia host defense by attenuating apoptosis, promoting cell survival, and maintaining nitric oxide and tight junction levels. It exerts its function through TLR4 activation to downregulate tight junction protein expression and TLR2 activation to accelerate apoptosis.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ju Zhao, Ye Zhao, Haifeng Liu, Quanquan Cao, Lin Feng, Zhihao Zhang, Weidan Jiang, Pei Wu, Yang Liu, Wei Luo, Xiaoli Huang, Jun Jiang
Summary: This study aimed to evaluate the possible impact and mechanism of leucine on fish intestinal barrier function. The results showed that the intestinal activities of certain enzymes and the levels of immune proteins had positive responses to dietary leucine levels. These findings suggest that leucine can improve fish intestinal barrier function by enhancing humoral immunity, antioxidative capacities, and tight junction protein levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Franziska Weiss, David Holthaus, Martin Kraft, Christian Klotz, Martina Schneemann, Joerg D. Schulzke, Susanne M. Krug
Summary: Duodenal barriers are often studied using limited cell lines, while organoids provide a better representation of the in vivo situation due to the inclusion of all intestinal cell types. Organoid-derived monolayers (ODMs) were developed to overcome the limitations of organoids. By analyzing tight junction composition and electrophysiological barrier properties, it was found that ODMs resemble the originating tissue more closely than Caco-2, a commonly used cell line. Transcriptomic data also supported the superior functionality of ODMs. In summary, ODMs are a suitable model for studying tissue-specific properties, offering reproducible functionality and a variety of cell types.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Immunology
Pradeep K. Shukla, Roshan G. Rao, Avtar S. Meena, Francesco Giorgianni, Sue Chin Lee, Preeti Raju, Nitesh Shashikanth, Chandra Shekhar, Sarka Beranova, Louisa Balazs, Gabor Tigyi, Ankush Gosain, Radhakrishna Rao
Summary: This study demonstrates that radiation induces dysfunction of Paneth cells in the intestine, and feeding HD5 prevents and mitigates radiation-induced intestinal mucosal injury, endotoxemia, and systemic inflammation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Yunika Mayangsari, Natsumi Sugimachi, Wenxi Xu, Chinatsu Mano, Yuki Tanaka, Osamu Ueda, Tomohiro Sakuta, Yoshiharu Suzuki, Yoshinari Yamamoto, Takuya Suzuki
Summary: This study demonstrated that 3,5,7,3',4'- pentamethoxyflavone (PMF) can enhance the intestinal tight junction (TJ) barrier integrity by regulating the transcription of TJ proteins, such as occludin, claudin-3, and claudin-4.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Cell Biology
K. M. Diguilio, E. Rybakovsky, M. C. Valenzano, H. H. Nguyen, E. A. Del Rio, E. Newberry, R. Spadea, J. M. Mullin
Summary: The study found that quercetin can improve airway epithelial cell barrier function and reduce barrier compromise caused by TNF-α. This improvement may be related to changes in tight junctional protein composition and inhibition of cell replication.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Darren Shu Jeng Ting, Jianguo Li, Chandra S. Verma, Eunice T. L. Goh, Mario Nubile, Leonardo Mastropasqua, Dalia G. Said, Roger W. Beuerman, Rajamani Lakshminarayanan, Imran Mohammed, Harminder S. Dua
Summary: CaD23 is a novel synthetic HDP with strong antimicrobial efficacy, especially in combination with antibiotics. Its rapid antimicrobial action is mainly attributed to its membrane-permeabilising mechanism, and MD simulations are useful for elucidating its mechanism of action and guiding design optimization.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Medicine, General & Internal
Darren Shu Jeng Ting, Imran Mohammed, Rajamani Lakshminarayanan, Roger W. Beuerman, Harminder S. Dua
Summary: Vision is considered the most important sense in humans, and the ocular surface plays a crucial role in defending the eye against environmental stress and pathogens. Host defense peptides (HDPs) are innate immune molecules that have antimicrobial and other biological functions. This review provides an updated overview of HDPs at the ocular surface, their involvement in ocular diseases/conditions, current developments in HDP-based treatments for ocular diseases and infections, and the future potential of HDP-based clinical pharmacotherapy for ocular diseases.
FRONTIERS IN MEDICINE
(2022)
Article
Immunology
Guangming Ren, Liming Xu, Jingzhuang Zhao, Yizhi Shao, Xiaoyu Chen, Tongyan Lu, Qiya Zhang
Summary: This study evaluated the effects of crude lentinan (CLNT) on the intestinal microbiota and the immune barrier in rainbow trout infected with infectious hematopoietic necrosis virus (IHNV). The results showed that supplementary CLNT reduced trout mortality caused by IHNV, strengthened the intestinal immune barrier, and modified the intestinal microbiota. Additionally, CLNT promoted the growth of short-chain fatty acid-producing bacteria and increased the production of certain fatty acids in the intestine of IHNV-infected trout. These findings suggest that CLNT has promising prebiotic properties in preventing IHNV infection in rainbow trout.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Veterinary Sciences
Rosanna Marsella, Rachel Wilkes, Kim Ahrens
Summary: This study compares the suitability of canine progenitor epidermal keratinocytes (CPEK) and normal keratinocytes (NK) in skin barrier studies. The results show that CPEK grown in monolayer exhibit differences in cell size, granularity, and tight junction expression compared to NK, indicating that they are not suitable for permeability studies.
VETERINARY SCIENCES
(2022)
Article
Multidisciplinary Sciences
Ilke De Boeck, Stijn Wittouck, Katleen Martens, Irina Spacova, Eline Cauwenberghs, Camille Nina Allonsius, Jennifer Joerissen, Sander Wuyts, Wannes Van Beeck, Jelle Dillen, Peter A. Bron, Brecht Steelant, Peter W. Hellings, Olivier M. Vanderveken, Sarah Lebeer
Summary: The study found Dolosigranulum pigrum to be a species clearly associated with nasal health, mainly present in the human nasal cavity. The strain exhibits antimicrobial activity, decreases inflammation, and protects nasal barrier function, showing potential as a topical live biotherapeutic product.