Article
Anatomy & Morphology
Carolina Muniz Partida, Eric Walters
Summary: Immunohistochemistry (IHC) is a widely used method for visualizing tissue architecture and protein expression. This study developed a new IHC protocol using paraffin embedded tissue, which overcomes the limitations of traditional methods and saves time and resources. The protocol successfully localized specific antigens in mouse brain tissue and expands the versatility of paraffin embedded tissues in various applications.
FRONTIERS IN NEUROANATOMY
(2023)
Article
Multidisciplinary Sciences
Neha Thakur, Debajyoti Chatterjee, Anubha Dev, Rahul Mahajan, Sanjeev Handa, Dipankar De
Summary: Immunohistochemistry (IHC) for C3d and C4d can help differentiate between bullous pemphigoid (BP) and mucous membrane pemphigoid (MMP). C3d has better diagnostic importance in BP, while C3d and C4d have lower diagnostic significance in MMP.
SCIENTIFIC REPORTS
(2023)
Article
Clinical Neurology
Marco Vabanesi, Anne-Laurie Pinto, Alberto Vogrig, David Goncalves, Veronique Rogemond, Bastien Joubert, Nicole Fabien, Jerome Honnorat, Sergio Muniz-Castrillo
Summary: This study aimed to describe the clinical associations of SOX1 antibodies, determine the accuracy of various detection techniques, and propose laboratory criteria for the diagnosis of paraneoplastic neurological syndromes associated with SOX1 antibodies. The study found that SOX1 antibodies should only be considered high-risk when detected with a positive antigen-specific test and immunofluorescence. Other laboratory results and clinical associations should be carefully reassessed to rule out false positivity and alternative diagnoses.
JOURNAL OF NEUROLOGY
(2023)
Article
Environmental Sciences
Kristen M. Prossner, Ellen Harvey, Michael A. Unger
Summary: Crassostrea virginica is a widely used bivalve species for biomonitoring organic pollutants in aquatic environments. This study investigates the differences in polycyclic aromatic hydrocarbon (PAH) kinetics between wild oysters, triploid and diploid cultured oysters. The findings suggest that equilibration time and internal partitioning of contaminants play important roles in the observed differences.
ENVIRONMENTAL MONITORING AND ASSESSMENT
(2023)
Article
Biochemistry & Molecular Biology
Giusy Maraventano, Giulio Ticli, Ornella Cazzalini, Lucia A. Stivala, Mariella Ramos-Gonzalez, Jose-Luis Rodriguez, Ennio Prosperi
Summary: The formation of 7,8-dihydro-8-oxo-2'-deoxyguanosine (8-oxodG) is an important biomarker of oxidative damage in cellular DNA. While antibodies recognizing 8-oxodG are available, the use of glycoprotein avidin for detection has also been proposed. This study compares the reliability and sensitivity of immunofluorescence determination using the monoclonal antibody N45.1 with labeling using avidin conjugated with the fluorochrome Alexa Fluor488 (AF488). The results indicate that the monoclonal antibody N45.1 provides a greater specificity in labeling 8-oxodG compared to avidin-AF488, suggesting that immunofluorescence techniques are best suited for in situ analysis of 8-oxodG as a biomarker of oxidative DNA damage.
Article
Neurosciences
Haozhe Zhang, Chen Tan, Xiaoyue Shi, Ji Xu
Summary: Autofluorescence is a critical factor to consider when designing experiments and interpreting results based on fluorescence-based techniques to study microglia.
Article
Biochemical Research Methods
Shahid Nazir, Richard P. G. Charlesworth, Pierre Moens, Priscilla F. Gerber
Summary: Autofluorescence in formalin-fixed and paraffin-embedded avian tissues limits the use of immunofluorescence staining techniques, with Sudan Black B identified as an effective method to reduce autofluorescence. However, specific fluorescent signals were not observed for FITC-labelled antibodies against immune cell markers in the study.
JOURNAL OF IMMUNOLOGICAL METHODS
(2021)
Review
Neurosciences
Wonjin Cho, Sehun Kim, Young-Gyun Park
Summary: Profiling molecular expression in situ allows for a comprehensive understanding of biological systems by integrating biomolecular and cellular features. While multiplexed immunofluorescence methods can visualize numerous proteins in thin tissue sections, the ability to perform this analysis in thick tissues or intact organs would provide a new dimension in diverse biological research and medical applications. This article reviews current multiplexed immunofluorescence methods and explores potential approaches and challenges for achieving 3D multiplexed immunofluorescence.
Review
Medical Laboratory Technology
John B. Mee
Summary: Autoimmune blistering diseases are rare disorders of the skin and mucous membranes, and their diagnosis and treatment require multiple detection methods such as immunofluorescence and enzyme-linked immunosorbent assay to accurately diagnose and monitor disease activity.
BRITISH JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Matteo Pedrazzoli, Manuela Medelin, Federica Marchiotto, Barbara Cisterna, Manuela Malatesta, Mario Buffelli
Summary: Utilizing multiple analysis techniques on a single sample is desirable in research to reduce animal use and maximize information. This study presents a simplified brain tissue staining procedure allowing for multiple analyses in the same sample without requiring special equipment or skills.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Article
Anatomy & Morphology
Marina Scardigli, Luca Pesce, Niamh Brady, Giacomo Mazzamuto, Vladislav Gavryusev, Ludovico Silvestri, Patrick R. Hof, Christophe Destrieux, Irene Costantini, Francesco S. Pavone
Summary: The study evaluated different tissue transformation approaches to achieve uniform labeling of all neurons in the human cerebral cortex. Ultimately, high-resolution 3D reconstruction of successfully clarified and stained samples was achieved using LSFM technology.
FRONTIERS IN NEUROANATOMY
(2021)
Article
Gastroenterology & Hepatology
Su Lin, Catarina Araujo, Andrew Hall, Rahul Kumar, Alexandra Phillips, Mohsin Hassan, Cornelius Engelmann, Alberto Quaglia, Rajiv Jalan
Summary: This study provides novel insights into the important role of liver biopsy in patients with severe indeterminate acute hepatitis (sIAH), suggesting that the presence of multilobular necrosis is an early indicator of poor prognosis.
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Chemistry, Analytical
Cynthia L. Schreiber, Dong-Hao Li, Bradley D. Smith
Summary: The study demonstrated that antibodies labeled with NIR heptamethine cyanine dye s775z outperformed commercially available NIR fluorophores in terms of brightness, photostability, and target specificity, making them highly suitable for sensitive NIR fluorescence detection and photon-intense methods.
ANALYTICAL CHEMISTRY
(2021)
Article
Cell Biology
Jie Liu, Yanmei Qi, Joshua Chao, Pranav Sathuvalli, Leonard Y. Lee, Shaohua Li
Summary: CREG1 is mainly localized to the endosomal-lysosomal compartment and plays an important role in macropinocytosis and clathrin-dependent endocytosis. It promotes acidification and increases lysosomal biogenesis in the endosomal-lysosomal compartment. Overexpression of CREG1 enhances autophagy and lysosome-mediated degradation, while knockdown or knockout has opposite effects.
Article
Immunology
Bharati Matta, Jenna Battaglia, Betsy J. Barnes
Summary: Neutrophil extracellular traps (NETs) are web-like structures formed by activated neutrophils that contain antimicrobial granular proteins, peptides, and enzymes. They can clear microorganisms and inhibit infections, but dysregulated NETs are associated with autoimmune diseases and COVID-19 severity. This study developed new methodologies, using multiplex ELISA and immunofluorescence, to quantify NETs in patient plasma.
FRONTIERS IN IMMUNOLOGY
(2022)
Editorial Material
Medicine, General & Internal
Ian D. Odell, Amer M. Zeidan, Terri L. Parker, Oscar R. Colegio, Antonio Subtil
Article
Dermatology
Young H. Lim, Ian D. Odell, Christine J. Ko, Keith A. Choate
Letter
Dermatology
Veronica J. Shi, Anjela Galan, Ian D. Odell, Jennifer N. Choi
Letter
Dermatology
Ian D. Odell, Evelyn Lilly, Katharine Reeve, Marcus W. Bosenberg, Leonard M. Milstone
News Item
Microbiology
Ian D. Odell, Richard A. Flavell
NATURE MICROBIOLOGY
(2016)
Correction
Genetics & Heredity
Ian D. Odell, Kheng Newick, Nicholas H. Heintz, Susan S. Wallace, David S. Pederson
Article
Genetics & Heredity
Ian D. Odell, Kheng Newick, Nicholas H. Heintz, Susan S. Wallace, David S. Pederson
Review
Cell Biology
Ian D. Odell, Susan S. Wallace, David S. Pederson
JOURNAL OF CELLULAR PHYSIOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Ian D. Odell, Joy-El Barbour, Drew L. Murphy, Julie A. Della-Maria, Joann B. Sweasy, Alan E. Tomkinson, Susan S. Wallace, David S. Pederson
MOLECULAR AND CELLULAR BIOLOGY
(2011)
Editorial Material
Cell Biology
Ian D. Odell, Richard A. Flavell
NATURE CELL BIOLOGY
(2019)
Correction
Cell Biology
Ian D. Odell, Richard A. Flavell
NATURE CELL BIOLOGY
(2019)
Article
Immunology
Ian D. Odell, Holly Steach, Stephen B. Gauld, Lauren Reinke-Breen, Jozsef Karman, Tracy L. Carr, Joseph B. Wetter, Lucy Phillips, Monique Hinchcliff, Richard A. Flavell
Summary: Epiregulin-EGFR signaling between dendritic cells and fibroblasts plays a critical role in maintaining elevated ECM production and accumulation in fibrotic tissue. Epiregulin is induced by type I interferon and activates EGFR on fibroblasts through NOTCH signaling, driving a positive feedback loop. Epiregulin is essential for persistence of fibrosis in skin and lung tissues, and therapeutic administration of epiregulin antibody can reverse fibrosis.
SCIENCE IMMUNOLOGY
(2022)
Article
Rheumatology
Catherine B. Xie, Shawn Cowper, Ian D. Odell
Summary: Morphea, also known as localized scleroderma, is characterized by inflammation and fibrosis of the skin. The exact pathogenesis is unknown, but may involve genetic predisposition and environmental factors. While some cases have been associated with Borrelia infection, it is not a generalizable cause for most patients.
JOURNAL OF SCLERODERMA AND RELATED DISORDERS
(2021)
Article
Cell Biology
B Hamilton, YQ Doug, M Shindo, WY Liu, I Odell, G Ruvkun, SS Lee
GENES & DEVELOPMENT
(2005)
