Article
Immunology
Jake R. Thomas, Anna Appios, Xiaohui Zhao, Roksana Dutkiewicz, Maria Donde, Colin Y. C. Lee, Praveena Naidu, Christopher Lee, Joana Cerveira, Bing Liu, Florent Ginhoux, Graham Burton, Russell S. Hamilton, Ashley Moffett, Andrew Sharkey, Naomi McGovern
Summary: Hofbauer cells are an important immune cell population found in the placenta, involved in placental angiogenesis and remodeling. Additionally, there is another population of maternal macrophages in the placenta that aid in surface repair.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Oncology
Anna Sandstrom Gerdtsson, Mattis Knulst, Johan Botling, Artur Mezheyeuski, Patrick Micke, Sara Ek
Summary: The immune microenvironment of NSCLC is heterogeneous, hindering the prediction of response to immune checkpoint inhibitors. Mapping the expression of 49 proteins in 33 NSCLC tumors revealed key differences in phenotype and function associated with the spatial context of immune infiltration. Tumor-infiltrating leukocytes (TIL) displayed high levels of immune suppressive markers, while stromal leukocytes (SL) expressed higher levels of T-cell activation marker CD27. Tertiary lymphoid structures (TLS) showed less variation in expression profile and higher levels of lymphocyte markers, dendritic cells, and antigen presentation.
Article
Biotechnology & Applied Microbiology
Jingsong Wang, Sally Roberts, Weiping Li, Karina Wright
Summary: This study identifies and characterizes progenitor cell populations in different regions of the human meniscus. Progenitor cells from the vascular region show enhanced proliferative and fibrochondrogenic characteristics compared to those from the avascular region. These findings indicate that meniscal progenitors may be a promising cell therapy strategy for meniscal regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Kaline de Brito Sousa, Daniela de Fatima Teixeira da Silva, Maria Fernanda Setubal Destro Rodrigues, Monica Pereira Garcia, Carolina de Oliveira Rodini, Raquel Agnelli Mesquita-Ferrari, Michael R. Hamblin, Sandra Kalil Bussadori, Fabio Daumas Nunes, Kristianne Porta Santos Fernandes
Summary: This study evaluated the optical absorbance spectrum of human monocytes, neutrophils and lymphocytes, and found that cells exhibit greater absorbance in certain regions of the spectrum. Inflammatory cells showed increased absorbance at specific wavelengths, while immunoregulatory polarization had little effect on absorption. The findings suggest new perspectives for using photobiomodulation in clinical settings based on wavelengths and the stage of inflammation.
JOURNAL OF BIOPHOTONICS
(2021)
Article
Biochemistry & Molecular Biology
Xinh-Xinh Nguyen, Matthew Sanderson, Kristi Helke, Carol Feghali-Bostwick
Summary: Pulmonary fibrosis, a significant cause of morbidity and mortality in fibroproliferative disorders like systemic sclerosis and idiopathic pulmonary fibrosis, involves overexpression of IGFBP-5. This study utilized a transgenic model to demonstrate the functional role of IGFBP-5 in fibrosis development, showing that transgenic mice expressing human IGFBP-5 could serve as a useful animal model for examining IGFBP-5 function in vivo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Optics
Marius Kroeger, Johannes Schleusener, Sora Jung, Maxim E. Darvin
Summary: The study proposed parameters to distinguish morphologically different collagen I structures in the skin and conducted experiments to validate the method. Through image analysis, automated classification of different collagen I structures can be achieved.
Reprint
Materials Science, Multidisciplinary
Andrei Pissarenko, Marc A. Meyers
Summary: Skin, as the primary protective barrier of the body, regulates body temperature and stores water and lipids for healthy metabolism. Research on the mechanical behavior and deformation mechanisms of skin has led to the development of experimental techniques and models for various applications.
PROGRESS IN MATERIALS SCIENCE
(2021)
Article
Cell Biology
Piper A. Robida, Clayton H. Rische, Netali Ben-Baruch Morgenstern, Rethavathi Janarthanam, Yun Cao, Rebecca A. Krier-Burris, Wouter Korver, Alan Xu, Thuy Luu, Julia Schanin, John Leung, Marc E. Rothenberg, Joshua B. Wechsler, Bradford A. Youngblood, Bruce S. Bochner, Jeremy A. O'Sullivan
Summary: Mast cells are tissue-resident cells that contribute to allergic diseases through excessive or inappropriate cellular activation and degranulation. Siglec-6, a receptor selectively expressed by mast cells, could be a promising target for therapeutic intervention. This study found that Siglec-6 is highly expressed by human mast cells and has a potent inhibitory effect on mast cell activation, making it a potential therapeutic target for mast cell-driven diseases.
Article
Multidisciplinary Sciences
Anna Pascual-Reguant, Ralf Koehler, Ronja Mothes, Sandy Bauherr, Daniela C. Hernandez, Ralf Uecker, Karolin Holzwarth, Katja Kotsch, Maximilian Seidl, Lars Philipsen, Werner Mueller, Chiara Romagnani, Raluca Niesner, Anja E. Hauser
Summary: ILCs are essential regulators of biological processes. The study combines multiplexed imaging and computational pipelines to identify tonsillar IRF4 (+) ILC3s and conserved stromal landmarks for ILC localization, offering a platform for future ILC research.
NATURE COMMUNICATIONS
(2021)
Article
Pediatrics
Nagoud Schukfeh, Amr Elyas, Dorothee Viemann, Benno M. Ure, Stephanie Froemmel, Joon-Keun Park, Joachim F. Kuebler, Gertrud Vieten
Summary: The study demonstrates that lineage marker expression of peritoneal macrophages in neonates differs from that in adults. The findings may provide insights into the complex interaction of neonatal tissue resident macrophages and potentially inform therapeutic approaches utilizing specific macrophage properties.
EUROPEAN JOURNAL OF PEDIATRIC SURGERY
(2021)
Article
Polymer Science
Ching-Cheng Huang, Ying-Ju Chen, Hsia-Wei Liu
Summary: A new combined method was employed to prepare nano-bioscaffolds with fibrous extracellular matrix using pre- and post-treatment of supercritical fluids. The resulting nano-bioscaffolds exhibited good histological properties and hemocompatibility, indicating their potential for medical applications.
Article
Cell Biology
Lizhe Zhuang, Rahul M. Visalakshan, Pritinder Kaur
Summary: Skin ageing is a complex process influenced by various factors, with a decline in the number and function of pericytes potentially playing a significant role in impaired skin regeneration.
Article
Biochemistry & Molecular Biology
Gilda Aiello, Francesca Rescigno, Marisa Meloni, Beatrice Zoanni, Giancarlo Aldini, Marina Carini, Alfonsina D'Amato
Summary: This study aimed to analyze the effect of carnosine on the proteome of UVA-irradiated human skin fibroblasts. The results indicate that UVA dysregulates Oxidative Phosphorylation, the Fibrosis Signaling Pathway, Glycolysis I and Nrf2-mediated Oxidative Stress Response. Carnosine provides a protective function against UVA radiation by activating the Nrf2 pathway and regulating the activation of structural proteins and collagen against UVA-induced changes in epithelial adhesion and wound healing signaling pathways.
Article
Biochemistry & Molecular Biology
Luke Funk, Kuan-Chung Su, Jimmy Ly, David Feldman, Avtar Singh, Brittania Moodie, Paul C. Blainey, Iain M. Cheeseman
Summary: Understanding the roles of essential genes in cellular processes is crucial for understanding cellular growth, proliferation, and function. This study used CRISPR-Cas9-based screening and microscopy imaging to identify the contributions of over 5,000 genes to cellular organization and morphology. Analysis of millions of cells revealed measurable phenotypes for over 90% of gene knockouts, providing insights into specific cellular processes. Clustering of phenotypic similarities further revealed co-functional genes and predicted gene functions and associations. The study also identified genes with functional contributions to chromosome segregation. This work provides a detailed resource exploring the consequences of disrupting core cellular processes.
Article
Veterinary Sciences
Koen M. Santifort, Elise Bertijn, Sofie F. M. Bhatti, Peter Leegwater, Andrea Fischer, Paul J. J. Mandigers
Summary: This retrospective study analyzed data of Border Collies with idiopathic epilepsy, revealing a correlation between the age of onset of the first epileptic seizure and the severity of epilepsy. The study also characterized the phenotype of idiopathic epilepsy in Border Collies and emphasized the significant impact of epilepsy on their quality of life.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Developmental Biology
Rene Reitermaier, Tanya Ayub, Julia Staller, Philip Kienzl, Nikolaus Fortelny, Pablo Augusto Vieyra-Garcia, Christof Worda, Christian Fiala, Clement Staud, Wolfgang Eppel, Anke Scharrer, Thomas Krausgruber, Adelheid Elbe-Buerger
Summary: This study describes a new workflow for isolating and expanding significant amounts of T cells from fetal human skin. The characteristics of T cells in fetal skin were determined using multiparametric flow cytometry and immunofluorescence techniques. The molecular state of these cells was characterized using single-cell transcriptomics and TCR repertoire profiling.
Article
Medicine, General & Internal
Denise Traxler, Pavla Krotka, Maria Laggner, Michael Mildner, Alexandra Graf, Berthold Reichardt, Ralph Wendt, Johann Auer, Bernhard Moser, Julia Mascherbauer, Hendrik Jan Ankersmit
Summary: This study found that patients aged 50-65 who underwent bioprosthetic aortic valve replacement (B-AVR) had lower long-term survival rates and higher risks compared to mechanical aortic valve replacement (M-AVR). B-AVR also had higher risks of reoperation and myocardial infarction. However, the risk of heart failure and stroke did not significantly differ after AVR in any age group.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Public, Environmental & Occupational Health
Tagrid Jar-Allah, Helena Hognert, Laura Kocher, Linus Berggren, Christian Fiala, Ian Milsom, Kristina Gemzell-Danielsson
Summary: The study aimed to evaluate the outcome of the very early medical abortion (VEMA) protocol and its potential to increase early diagnosis and treatment of an asymptomatic ectopic pregnancy (EP). The results showed a high success rate of VEMA, providing an opportunity for early treatment, but the occurrence of EP was very low.
EUROPEAN JOURNAL OF CONTRACEPTION AND REPRODUCTIVE HEALTH CARE
(2022)
Article
Multidisciplinary Sciences
Maria Laggner, Marie-Therese Lingitz, Dragan Copic, Martin Direder, Katharina Klas, Daniel Bormann, Alfred Gugerell, Bernhard Moser, Christine Radtke, Stefan Hacker, Michael Mildner, Hendrik Jan Ankersmit, Thomas Haider
Summary: Burn injuries elicit a unique stress response and can lead to burn injury progression. This study found that levels of neutrophil-derived factors were significantly elevated in burn victims, and leukocyte and neutrophil counts were increased while lymphocytes were decreased. These factors did not predict mortality, but were associated with higher burn severity scores in patients with third-degree burns.
SCIENTIFIC REPORTS
(2022)
Article
Public, Environmental & Occupational Health
Christian Fiala, Aubert Agostini, Teresa Bombas, Roberto Lertxundi, Marek Lubusky, Mirella Parachini, Kristina Gemzell-Danielsson
Summary: This article investigates abortion legislation and statistics in 32 European countries and finds that legislation varies widely and abortion rates differ significantly among countries, with no clear correlation between laws and rates. These findings are important for understanding the needs of women deciding to have an abortion and for potentially changing laws to better meet those needs.
EUROPEAN JOURNAL OF CONTRACEPTION AND REPRODUCTIVE HEALTH CARE
(2022)
Article
Biochemistry & Molecular Biology
Martin Direder, Tamara Weiss, Dragan Copic, Vera Vorstandlechner, Maria Laggner, Karin Pfisterer, Caterina Selina Mildner, Katharina Klas, Daniel Bormann, Werner Haslik, Christine Radtke, Matthias Farlik, Lisa Shaw, Bahar Golabi, Erwin Tschachler, Konrad Hoetzenecker, Hendrik Jan Ankersmit, Michael Mildner
Summary: This study identifies a unique population of Schwann cells in keloids and highlights the interaction between Schwann cells and macrophages in keloid formation.
Article
Biology
Maria Laggner, Felicitas Oberndorfer, Bahar Golabi, Jonas Bauer, Andreas Zuckermann, Philipp Hacker, Irene Lang, Nika Skoro-Sajer, Christian Gerges, Shahrokh Taghavi, Peter Jaksch, Michael Mildner, Hendrik Jan Ankersmit, Bernhard Moser
Summary: This study investigated the gene expression profiles of hypertrophic and regenerated right hearts of two subtypes of pulmonary hypertension (PH) patients. The findings revealed unique gene alteration signatures in different PH subtypes, as well as the identification of a profibrotic factor, early growth response 1 (EGR1), in both subtypes. These findings may contribute to the identification of therapeutic candidates for PH patients who are not eligible for surgical treatment.
Article
Medicine, General & Internal
Maria Laggner, Gabriela Sanchez-Acosta, Claudia Kitzmuller, Dragan Copic, Florian Gruber, Lukas Matthaus Altenburger, Vera Vorstandlechner, Alfred Gugerell, Martin Direder, Katharina Klas, Daniel Bormann, Anja Peterbauer, Akira Shibuya, Barbara Bohle, Hendrik Jan Ankersmit, Michael Mildner
Summary: This study demonstrates that the secretome of g-irradiated peripheral blood mononuclear cells (PBMCsec) can alleviate allergic reactions by reducing the activation of mast cells and basophils. The findings suggest the potential use of PBMCsec as a treatment for allergies.
Article
Pharmacology & Pharmacy
Dragan Copic, Martin Direder, Klaudia Schossleitner, Maria Laggner, Katharina Klas, Daniel Bormann, Hendrik Jan Ankersmit, Michael Mildner
Summary: This study investigates the tissue-regenerative properties of paracrine factors released from stem cells and other cell types. The secretome of gamma-irradiated PBMCs has been shown to possess tissue-regenerative and proangiogenic capacities. The study demonstrates that PBMCsec induces angiogenic and proteolytic processes in the blood and is able to attenuate endothelial barrier damage. These findings suggest that systemic application of PBMCsec might be a promising novel strategy to restore damaged organs.
Article
Biochemistry & Molecular Biology
Katharina Klas, Anna S. Ondracek, Thomas M. Hofbauer, Andreas Mangold, Karin Pfisterer, Maria Laggner, Dragan Copic, Martin Direder, Daniel Bormann, Hendrik Jan Ankersmit, Michael Mildner
Summary: This study found that PBMCsec can significantly inhibit the formation of NETs, possibly through antioxidant activity and inhibition of protein arginine deiminase 4 activity. For the current lack of treatment options for excessive NET formation, this study provides a promising novel treatment option.
Article
Cell Biology
Dragan Copic, Martin Direder, Katharina Klas, Daniel Bormann, Maria Laggner, Hendrik Jan Ankersmit, Michael Mildner
Summary: This study reveals a novel mode of action of ATG in regulating T cell activity and effector functions through the secretion of cytokines and chemokines, as well as the modulation of cell surface ligands and receptors.
Review
Dermatology
Daniel Bormann, Alfred Gugerell, Hendrik Jan Ankersmit, Michael Mildner
Summary: Cell secretomes play an important role in wound healing by promoting regeneration. This study reviews the mechanisms of action of cell secretomes in wound healing, important preconditioning strategies to enhance therapeutic efficacy, and clinical trials on secretome-based wound healing.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Cell Biology
Zahra Kargarpour, Layla Panahipour, Michael Mildner, Richard J. Miron, Reinhard Gruber
Summary: Platelet-rich fibrin (PRF) has potent anti-inflammatory activity, however, the components responsible for this effect are unknown. In this study, we investigated whether the lipid fraction of PRF also exhibits anti-inflammatory properties. Our results show that lipids from PRF can significantly reduce the inflammatory response of ST2 bone marrow stromal cells and primary bone marrow macrophages. These findings suggest that the lipid fraction is at least partially responsible for the anti-inflammatory activity of PRF in vitro.
Article
Pharmacology & Pharmacy
Vera Vorstandlechner, Dragan Copic, Katharina Klas, Martin Direder, Bahar Golabi, Christine Radtke, Hendrik J. Ankersmit, Michael Mildner
Summary: This study demonstrates the anti-fibrotic effect of PBMCsec on cutaneous scars in both mouse and human experimental settings. PBMCsec regulates the expression of fibrosis-related genes, attenuates myofibroblast differentiation, and inhibits the breakdown of elastic fibers. These findings suggest that PBMCsec could be a novel therapeutic option for treating skin scarring.
Article
Psychology, Multidisciplinary
Anna Felnhofer, Jennifer Kernreiter, Claudia Klier, Mercedes M. Huscsava, Christian Fiala, Michael Zeiler
Summary: Research on adoptive parents of anonymously born children is lacking. This study compared mental health, attachment styles, dyadic coping, and biographical knowledge between adoptive mothers and fathers, and identified predictors of dyadic coping. Findings revealed low biographical knowledge in adoptive parents, with mothers reporting more anxiety and higher ability to communicate stress and cope dyadically. Knowledge of more biographical data, older age of parents, younger age of child, and higher psychopathology scores predicted better dyadic coping in adoptive parents.
CURRENT PSYCHOLOGY
(2023)