Article
Multidisciplinary Sciences
Yuan Zhang, Scheffer C. G. Tseng, Ying-Ting Zhu
Summary: The study showed that culturing trabecular meshwork (TM) cells under different matrix conditions resulted in varying phenotypes, with 3D Matrigel assisting in maintaining the TM phenotype and being regulated by TGF-beta. Abnormal matrices may contribute to perpetuating pathological TM phenotypes in glaucoma patients with elevated levels of TGF-beta 2.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Wei-Chen Yen, Kai-Ping Chang, Cheng-Yi Chen, Yenlin Huang, Ting-Wen Chen, Hsing-Wen Cheng, Jui-Shan Yi, Chun-Chia Cheng, Chih-Ching Wu, Chun- Wang
Summary: By comparing DEGs in OSCC-TCGA patients with CNVs identified in OSCC-OncoScan dataset, we found 37 dysregulated candidate genes, 26 of which have been reported in HNSCC. Among 11 novel candidates, MFI2 was identified as the most significant prognostic molecular in OSCC. Knockdown of MFI2 reduced cell viability, migration, and invasion in OSCC cells through modulation of EGF/FAK signaling.
CANCER CELL INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Man Lee Yuen, Ling Zhuang, Emma M. Rath, Takun Yu, Ben Johnson, Kadir Harun Sarun, Yiwei Wang, Steven Kao, Anthony Linton, Candice Julie Clarke, Brian C. McCaughan, Ken Takahashi, Kenneth Lee, Yuen Yee Cheng
Summary: Research on the role of E-cadherin and microRNA in the efficacy of FAK inhibitors in MPM revealed that MPM cells with high CDH1 mRNA levels exhibited resistance to the FAK inhibitor PND-1186. Additionally, PND-1186 induced cell cycle disruption by inducing the G2/M arrest of MPM cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Shijie Shao, Lianhua Piao, Liwei Guo, Jiangsong Wang, Luhui Wang, Jiawen Wang, Lei Tong, Xiaofeng Yuan, Junke Zhu, Sheng Fang, Yimin Wang
Summary: This study demonstrates that Tspan7 promotes osteosarcoma (OS) metastasis by interacting with beta 1 integrin and activating the FAK-Src-Ras-ERK1/2 pathway. Tspan7 is highly expressed in primary OS tumors and cell lines, and downregulation of Tspan7 suppresses OS growth, metastasis, and epithelial-mesenchymal transition (EMT). This study provides a potential therapeutic strategy for OS.
CANCER CELL INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Linlin Yang, Changxian Shen, Adriana Estrada-Bernal, Ryan Robb, Moumita Chatterjee, Nikhil Sebastian, Amy Webb, Xiaokui Mo, Wei Chen, Sunil Krishnan, Terence M. Williams
Summary: This study found that KRAS mutations lead to radioresistance by activating the NRF2-53BP1 axis in DNA repair and tumor cell survival after radiotherapy. Targeting NRF2, 53BP1, or NHEJ may represent novel strategies to selectively abrogate KRAS mutation-mediated radioresistance.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Developmental Biology
Lenin Chandran, Wilko Backer, Raphael Schleutker, Deqing Kong, Seyed A. H. Beati, Stefan Luschnig, H. -Arno J. Mueller
Summary: This study investigates the function of Src42A in junction remodeling during Drosophila gastrulation. Src42A is involved in tyrosine phosphorylation at bicellular and tricellular junctions in germband cells and localizes to areas of mechanical tension. The study demonstrates that Src42A is required for junction contraction and planar polarity of E-cadherin, and it acts in concert with Abl kinase in cell intercalations.
Article
Oncology
P. Brlek, A. Bukovac, A. Kafka, N. Pecina-Slaus
Summary: This study investigated the expression of E-cadherin and its transcriptional repressor TWIST1 in brain metastases originating from breast and lung cancer. The results showed a significant negative correlation between TWIST1 and E-cadherin expression levels in brain metastases.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2021)
Article
Physiology
Aswathy M. Cheriyan, Adaku C. Ume, Cynthia E. Francis, Keyona N. King, Valerie A. Linck, Yun Bai, Hui Cai, Robert S. Hoover, Heping P. Ma, Jennifer L. Gooch, Clintoria R. Williams
Summary: CnA alpha deficiency stimulates Nox2 upregulation, and NF-kappa B serves as a novel CnA alpha-regulated transcription factor that mediates Nox2 and ROS regulation. These findings suggest potential for selective CNIs to improve long-term outcomes by mitigating renal side effects.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Oncology
Yuji Kumagai, Junko Nio-Kobayashi, Seiichiro Ishihara, Atsushi Enomoto, Masashi Akiyama, Ryosuke Ichihara, Hisashi Haga
Summary: “Collective invasion of cancer cells as a cell cluster is associated with metastasis and worse prognosis. In this study, different subclones with varying invasive potentials were established from human skin squamous carcinoma cells. The highly invasive subclone exhibited a hermetically sealed and narrow intercellular space, localized interferon-beta, and activated STAT1, leading to collective invasion. On the other hand, the low-invasive subclone with deficient STAT1 activity had non-sealed and wide intercellular spaces. The coexistence of high- and low-invasive subclones resulted in collective invasion with the high-invasive cells leading the invasion front. Tissue microarray analysis further supported the role of STAT1 in collective invasion in human skin squamous cell carcinoma.”
Article
Oncology
Yunhee Lee, Dongjoon Ko, Junghwa Yoon, Younghoon Lee, Semi Kim
Summary: TMEM52B has been identified as a novel gene expressed widely in normal human tissues. Suppression of TMEM52B in colon cancer cells leads to enhanced epithelial-mesenchymal transition (EMT), invasion, and survival, potentially through multiple signaling pathways. Clinical data suggest that high TMEM52B expression is associated with increased patient survival in various types of cancer.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Oncology
James Drury, Piotr G. Rychahou, Courtney O. Kelson, Mariah E. Geisen, Yuanyuan Wu, Daheng He, Chi Wang, Eun Y. Lee, B. Mark Evers, Yekaterina Y. Zaytseva
Summary: Colorectal cancer is the second leading cause of cancer-related death in the world. The upregulation of CD36 and MMP28 has been associated with colorectal cancer metastasis. This study found that CD36 promotes colorectal cancer invasion and metastasis by upregulating MMP28 expression and enhancing the cleavage of E-cadherin.
Article
Biochemistry & Molecular Biology
Zongtao Ren, Yunfeng Niu, Bo Fan, Aili Zhang
Summary: HOXD10 acts as a tumor suppressor in CCRCC, suppressing invasion and migration of cancer cells by regulating E-cadherin and EMT processes. Targeting HOXD10 may be a promising therapeutic strategy for CCRCC treatment.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Yingmin Liu, Xinya Liu, Fei Chen, Wei Nian, Xiaotong Huang, Qianqian Yang, Songyu Hou, Zhiqin Fan
Summary: This study discovered that LOXL2 is highly expressed in ESCC tumors and its positive expression is associated with poor tumor differentiation, lymph node metastases, and poor prognosis in ESCC patients. In vitro experiments showed that LOXL2 promotes ESCC cell proliferation, migration, and invasion, and induces EMT by altering the expression of EMT markers. Mechanistically, LOXL2 activates the FAK/Src signaling pathway in ESCC. Therefore, LOXL2 may serve as a marker of poor prognosis and a potential therapeutic target for the treatment of esophageal squamous carcinoma.
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yaxuan Hou, Meng Zhou, Yuncheng Li, Tingting Tian, Xun Sun, Mo Chen, Wenmao Xu, Meixia Lu
Summary: Long noncoding RNAs (lncRNAs) play a role in the tumorigenesis of various tumors, but there is insufficient research on their association with the risk of head and neck squamous cell carcinoma (HNSCC). This study found that two SNPs (rs16854802 A > G and rs3113503 G > C) in LINC01614 increased the risk of HNSCC and disrupted the binding between LINC01614 and miRNA-616-3p, leading to increased expression of LINC01614. Additionally, upregulated LINC01614 was associated with poor prognosis in HNSCC patients, and knocking down LINC01614 inhibited the proliferation, invasion, and migration of HNSCC cells.
MOLECULAR CARCINOGENESIS
(2022)
Article
Biochemistry & Molecular Biology
Lorena Lobos-Gonzalez, Lorena Orostica, Natalia Diaz-Valdivia, Victoria Rojas-Celis, America Campos, Eduardo Duran-Jara, Nicole Farfan, Lisette Leyton, Andrew F. G. Quest, Takuji Tanaka, Masahito Shimizu, Michihiro Mutoh
Summary: The co-expression of CAV1 and E-cadherin can modulate the function of melanoma cells, but under the influence of PGE2, E-cadherin is unable to suppress CAV1-enhanced tumor metastasis and tyrosine-14 phosphorylation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Letter
Urology & Nephrology
Scott Wilkinson, Huihui Ye, Rosina T. Lis, Adam G. Sowalsky
Article
Oncology
Fen Ma, Seiji Arai, Keshan Wang, Carla Calagua, Amanda R. Yuan, Larysa Poluben, Zhongkai Gu, Joshua W. Russo, David J. Einstein, Huihui Ye, Meng Xiao He, Yu Liu, Eliezer Van Allen, Adam G. Sowalsky, Manoj K. Bhasin, Xin Yuan, Steven P. Balk
Summary: This study reveals the importance of Wnt/beta-catenin signaling in prostate cancer, showing its role in stem cell maintenance and invasion. It identifies new effectors and drivers of this pathway in prostate cancer, such as ROR1 and APC genomic loss. The findings suggest that targeting Wnt/beta-catenin signaling may be a potential therapeutic strategy for prostate cancer.
Article
Oncology
Xin Dong, Hui Xue, Fan Mo, Yen-Yi Lin, Dong Lin, Nelson K. Y. Wong, Yingqiang Sun, Scott Wilkinson, Anson T. Ku, Jun Hao, Xinpei Ci, Rebecca Wu, Anne Haegert, Rebecca Silver, Mary-Ellen Taplin, Steven P. Balk, Joshi J. Alumkal, Adam G. Sowalsky, Martin Gleave, Colin Collins, Yuzhuo Wang
Summary: This study aimed to develop clinically relevant models for studying ADT-induced prostate cancer dormancy. The researchers established 11 ADT-induced dormant prostate cancer models that closely mimicked the clinical courses of ADT-treated prostate cancer and identified two ADT-induced dormancy subtypes that differed in morphology, gene expression, and relapse rates. They also discovered transcriptomic differences in precastration PDXs that predisposed the dormancy response to ADT and developed a dormancy subtype-based, predisposed gene signature that was significantly associated with ADT response in hormonal naive prostate cancer and clinical outcome in castration-resistant prostate cancer treated with ADT or androgen-receptor pathway inhibitors.
MOLECULAR CANCER RESEARCH
(2022)
Article
Urology & Nephrology
John R. Bright, Rosina T. Lis, Anson T. Ku, Nicholas T. Terrigino, Nichelle C. Whitlock, Shana Y. Trostel, Nicole Carrabba, Stephanie A. Harmon, Baris Turkbey, Scott Wilkinson, Adam G. Sowalsky
Summary: The study evaluated the ability of antibodies against PSMA to specifically detect residual tumor in patients treated with iADT and enzalutamide. The results showed that PSMA reacted positively with tumor in all cases and had high sensitivity but low specificity for benign regions. PSMA could also identify highly dedifferentiated prostate carcinomas, indicating its potential as a valuable marker.
JOURNAL OF UROLOGY
(2022)
Editorial Material
Oncology
Scott Wilkinson, Adam G. Sowalsky
TRANSLATIONAL ONCOLOGY
(2022)
Article
Oncology
Adam G. Sowalsky, Ines Figueiredo, Rosina T. Lis, Ilsa Coleman, Bora Gurel, Denisa Bogdan, Wei Yuan, Joshua W. Russo, John R. Bright, Nichelle C. Whitlock, Shana Y. Trostel, Anson T. Ku, Radhika A. Patel, Lawrence D. True, Jonathan Welti, Juan M. Jimenez-Vacas, Daniel Nava Rodrigues, Ruth Riisnaes, Antje Neeb, Cynthia T. Sprenger, Amanda Swain, Scott Wilkinson, Fatima Karzai, William L. Dahut, Steven P. Balk, Eva Corey, Peter S. Nelson, Michael C. Haffner, Stephen R. Plymate, Johann S. de Bono, Adam Sharp
Summary: This study demonstrates that further analytical validation and clinical qualification are required before AR-V7 can be considered for clinical use in CSPC as a predictive biomarker.
CLINICAL CANCER RESEARCH
(2022)
Article
Oncology
Jennifer Wilkinson Carlisle, Caroline S. Jansen, Maria Andrea Cardenas, Ewelina Sobierajska, Adriana Moon Reyes, Rachel Greenwald, Luke Del Balzo, Nataliya Prokhnevska, Omer Kucuk, Bradley C. Carthon, Patrick Connor Mullane, Adeboye Osunkoya, Deborah Baumgarten, Fares Hosseinzadeh, Scott Wilkinson, Ross Lake, Adam G. Sowalsky, Yuan Liu, Viraj A. Master, Mehmet A. Bilen, Haydn Kissick
Summary: This study identifies potential immunological biomarkers that can predict therapeutic response in patients with advanced renal cell carcinoma (RCC). Assessing changes in T cells in the peripheral blood and tumor microenvironment, the researchers found that patients with the highest increase in specific activated T cells had the best antitumor immune response and clinical benefit.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Editorial Material
Oncology
David Y. Takeda, Adam G. Sowalsky
Summary: A recent report in Nature demonstrates that whole-genome sequencing of circulating tumor DNA from patients with metastatic PCa can inform on the subclonal composition of metastatic disease and infer patterns of gene expression.
Letter
Urology & Nephrology
Adam G. Sowalsky, Stephen R. Plymate, Michael C. Haffner, Johann S. de Bono, Adam Sharp
Article
Biology
Alexandre A. Germanos, Sonali Arora, Ye Zheng, Erica T. Goddard, Ilsa M. Coleman, Anson T. Ku, Scott Wilkinson, Hanbing Song, Nicholas J. Brady, Robert A. Amezquita, Michael Zager, Annalysa Long, Yu Chi Yang, Jason H. Bielas, Raphael Gottardo, David S. Rickman, Franklin W. Huang, Cyrus M. Ghajar, Peter S. Nelson, Adam G. Sowalsky, Manu Setty, Andrew C. Hsieh
Summary: In this study, we found that the expansion of castration-resistant intermediate luminal cells is associated with treatment resistance and poor prognosis in prostate cancer. The transformed epithelial cells and associated fibroblasts create a microenvironment that promotes pro-tumorigenic immune infiltration, which is influenced by androgen levels. Androgen-independent prostate cancer leads to significant diversification of intermediate luminal cell populations with varying androgen signaling activities, which are inversely correlated with proliferation and mRNA translation. The findings suggest that targeting translation inhibition could potentially lead to epithelial cell death, loss of pro-tumorigenic signaling, and decreased tumor heterogeneity.
Editorial Material
Oncology
Scott Wilkinson, Adam G. Sowalsky
JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE
(2023)
Meeting Abstract
Oncology
Caroline Jansen, Prasanthi Chappa, Nataliya Prokhnevska, Maria Cardenas, Roshan Prabhu, Jim Zhong, Kimberly Hoang, Subir Goyal, Suzanna Logan, Jeffrey Olson, Edjah Nduom, Luke del Balzo, Kirtesh Patel, Stuart Burri, Anthony Asher, Scott Wilkinson, Ross Lake, Kristin Higgins, Pretesh Patel, Vishal Dhere, Mylin Torres, Adam Sowalsky, Mohammad Khan, Haydn Kissick, Zachary Buchwald
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Oncology
Nichelle C. Whitlock, Margaret E. White, Brian J. Capaldo, Anson T. Ku, Supreet Agarwal, Lei Fang, Scott Wilkinson, Shana Y. Trostel, Zhen-Dan Shi, Falguni Basuli, Karen Wong, Elaine M. Jagoda, Kathleen Kelly, Peter L. Choyke, Adam G. Sowalsky
Summary: Our study reveals that the epigenetic activity of transcription factor oncogenes undergoes a shift during prostate cancer progression, leading to distinctive effects on metabolism. These epigenetically-driven changes in lipid metabolism may serve as novel targets for the development of imaging agents and therapeutics.
Meeting Abstract
Oncology
Rahim Hirani, Subhiksha Nandakumar, Teja Kalidindi, Deborah Fidele, Harisha Rajanala, Ying Mazzu, Yuki Yoshikawa, Lina Jehane, Gwo-Shu Mary Lee, Elisa de Stanchina, Adam Sowalsky, Michael J. Morris, Heiko Schoder, Naga Vara Kishore Pillarsetty, Lorelei A. Mucci, Daniel Danila, Goutam Chakraborty, Philip W. Kantoff
Meeting Abstract
Oncology
Caroline S. Jansen, Luke del Balzo, Roshan Prabhu, Suzanna Logan, Prasanthi Chappa, Kirtesh Patel, Scott Wilkinson, Ross Lake, Hui-Kuo G. Shu, Jim Zhong, Vishal Dhere, Jeffrey Olson, Adam G. Sowalsky, Mohammad K. Khan, Haydn T. Kissick, Zachary S. Buchwald
CLINICAL CANCER RESEARCH
(2021)