Article
Cell & Tissue Engineering
Yuyang Gan, Hailin Wang, Lijuan Du, Zhexiang Fan, Pingping Sun, Kaitao Li, Qian Qu, Jin Wang, Ruosi Chen, Zhiqi Hu, Yong Miao
Summary: A large number of pelage HF-MSCs can be quickly and conveniently isolated from adult mice using a two-step Ficoll density gradient sedimentation method. These cells possess similar characteristics and functions as whisker HF-MSCs and can promote hair growth by secreting exosomes.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Jung Min Park, Mee Sook Jun, Jung-A. Kim, Nanda Maya Mali, Tsai-Ching Hsi, Areum Cho, Jung Chul Kim, Jun Young Kim, Incheol Seo, Jungmin Kim, Moonkyu Kim, Ji Won Oh
Summary: This study demonstrated that inflammatory factors induce the expression of major histocompatibility complex class I proteins in dermal papilla cells, and the loss of immune privilege of hair follicles can be rescued by treatment with conditioned media from outer root sheath cells. Transcriptomic analyses identified 58 up-regulated genes and 183 down-regulated genes related to MHC class I+ cells.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Multidisciplinary Sciences
Carla M. Abreu, Mariana T. Cerqueira, Rogerio P. Pirraco, Luca Gasperini, Rui L. Reis, Alexandra P. Marques
Summary: The conditioned medium from interfollicular keratinocytes (KCs-CM) has been shown to improve the hair inductive ability of DP cells, with significant effects on DP cell growth and restoration of activity. Additionally, DP cells cultured with KCs-CM in spheroids demonstrated superior activity and supported the formation of hair follicle- and sebaceous gland-like structures in mice when co-grafted with human keratinocytes.
JOURNAL OF ADVANCED RESEARCH
(2021)
Review
Cell Biology
Chinnavuth Vatanashevanopakorn, Thanutchaporn Sartyoungkul
Summary: Hair follicles are involved in a variety of functions and their formation and cycling require interaction between different cell types. Despite extensive research, generating functional human hair follicles for clinical use remains a challenge. Human pluripotent stem cells offer a promising unlimited cell source for generating hair follicle cells. This review discusses hair follicle morphogenesis, different cell sources for regeneration, and potential strategies using induced pluripotent stem cells for hair follicle bioengineering. Challenges and perspectives for using bioengineered hair follicles in treating hair loss disorders are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell & Tissue Engineering
Carla M. Abreu, Rogerio P. Pirraco, Rui L. Reis, Mariana T. Cerqueira, Alexandra P. Marques
Summary: This study found that human interfollicular epidermal keratinocytes with stem-like features can replace human hair follicular keratinocytes for the recreation of the hair follicle epithelium and interactions, contributing to hair follicle development.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Longshuai Lin, Enjun He, Hongjie Wang, Weihong Guo, Zhenkai Wu, Kai Huang, Qinghua Zhao
Summary: The study demonstrates that intravenous transplantation of human hair follicle-derived mesenchymal stem cells (hHF-MSCs) can improve trabecular bone mass in osteoporotic mice by enhancing bone formation and inhibiting bone resorption. This effect may be mediated by various cytokines including OPG, Wnt2b, VCAM-1, Noggin, and RANKL. The findings provide insights for potential clinical applications in treating osteoporosis with hHF-MSCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Simon C. de Groot, Magda M. W. Ulrich, Coen G. Gho, Margriet A. Huisman
Summary: Hair disorders and severe burns can negatively impact an individual's social and psychological well-being. Studying human hair follicle development is crucial for wound healing and preventing scar formation, but research is limited and future focus should be on developing human disease-specific models.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Medicine, General & Internal
Mei-Qi Mao, Jing Jing, Yu-Jie Miao, Zhong-Fa Lv
Summary: Interactions between epithelial and mesenchymal cells have an impact on hair follicle development and skin wound healing. These interactions involve the exchange of soluble molecules, alteration of key pathways, and extracellular matrix signal transduction. This study summarizes the typical signaling molecules and extracellular components involved in epithelial-mesenchymal interaction, aiming to enhance our understanding of the mechanisms behind hair follicle regeneration and skin wound healing.
FRONTIERS IN MEDICINE
(2022)
Article
Engineering, Biomedical
Mafalda Correia, Joana Lopes, Daniela Lopes, Ana Melero, Pooyan Makvandi, Francisco Veiga, Jorge F. J. Coelho, Ana C. Fonseca, Ana Claudia Paiva-Santos
Summary: This review discusses nanotechnology-based strategies for hair follicle (HF) regeneration, including controlled drug release and HF-specific targeting. In addition to traditional hair repair methods, wound-induced hair neogenesis (WIHN) has gained attention as a research focus. HF regeneration involves various modalities and technologies, including modulation of the hair cycle, stimulation of progenitor cells and signaling pathways, tissue engineering, WIHN, and gene therapy.
Review
Dermatology
Subin Jeong, Yoojin Na, Hyeon-Min Nam, Gun Yong Sung
Summary: This article mainly introduces the research and treatment progress of hair, emphasizes the limitations of current hair follicle research models, and proposes the necessity to construct an in vitro culture system similar to the in vivo environment. It also discusses the development direction of hair follicle chips.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Dermatology
Koji Sugawara, Nora Zakany, Stephan Tiede, Talveen Purba, Matthew Harries, Daisuke Tsuruta, Tamas Biro, Ralf Paus
Summary: Research has shown that selective activation of the cannabinoid receptor-1 pathway significantly increases the number and proliferation of human epithelial stem cells in scalp hair follicles, while also promoting apoptosis in their differentiated progeny. This highlights the crucial role of CB1 signaling in the survival of adult human scalp hair follicle stem cells within their niche.
EXPERIMENTAL DERMATOLOGY
(2021)
Article
Multidisciplinary Sciences
Ko Tsutsui, Hiroki Machida, Asako Nakagawa, Kyungmin Ahn, Ritsuko Morita, Kiyotoshi Sekiguchi, Jeffrey H. Miner, Hironobu Fujiwara
Summary: This study systematically defines the spatial heterogeneity of skin basement membrane composition, highlighting its functional importance in distinct inter-tissue interactions. The basement membrane in the epithelial-fibroblast interface shows asymmetrical organized side-specific heterogeneity, which is crucial for hair cycle regulation and hair germ-dermal papilla anchoring.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Matej Znidaric, Zan Michel Zurga, Uros Maver
Summary: The field of hair research has advanced significantly in recent decades, particularly in the development of in vitro hair follicle models. The focus has been on manipulating cellular and molecular features to grow functional in vitro hair follicles. Challenges still remain in fully understanding follicle behavior and successfully creating different types of hair through in vitro methods.
Article
Cell Biology
Lihong Wen, Yong Miao, Zhexiang Fan, Jiarui Zhang, Yixuan Guo, Damao Dai, Junfei Huang, Zhen Liu, Ruosi Chen, Zhiqi Hu
Summary: This study successfully established an efficient primary culture system for hHFSCs using human fibronectin and the ROCK inhibitor Y-27632, which promoted proliferation, maintained stemness, and enhanced the regenerative capacity of hHFSCs in vivo. The xenofree culturing method provided high-quality seed cells with applications in hair follicle tissue engineering and stem cell therapy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Dermatology
Jiyoon Lee, Karl R. Koehler
Summary: Culturing skin cells outside the body has long been essential to dermatological research, but artificial skin equivalents often lack the full complexity of native skin, especially in generating and maintaining skin appendages such as hair follicles and sweat glands.
EXPERIMENTAL DERMATOLOGY
(2021)
Article
Cell Biology
Manuel Koch, Hans-Joachim Mollenkopf, Thomas F. Meyer
CELLULAR MICROBIOLOGY
(2016)
Article
Microbiology
Maria M. Esterhuyse, January Weiner, Etienne Caron, Andre G. Loxton, Marco Iannaccone, Chandre Wagman, Philippe Saikali, Kim Stanley, Witold E. Wolski, Hans-Joachim Mollenkopf, Matthias Schick, Ruedi Aebersold, Heinz Linhart, Gerhard Walzl, Stefan H. E. Kaufmann
Article
Multidisciplinary Sciences
Mirjana Kessler, Karen Hoffmann, Volker Brinkmann, Oliver Thieck, Susan Jackisch, Benjamin Toelle, Hilmar Berger, Hans-Joachim Mollenkopf, Mandy Mangler, Jalid Sehouli, Christina Fotopoulou, Thomas F. Meyer
NATURE COMMUNICATIONS
(2015)
Article
Engineering, Biomedical
Tomas Herkel, Radovan Uvizl, Lenka Doubravska, Milan Adamus, Tomas Gabrhelik, Miroslava Htoutou Sedlakova, Milan Kolar, Vojtech Hanulik, Vendula Pudova, Katerina Langova, Roman Zazula, Tomas Rezac, Michal Moravec, Pavel Cermak, Pavel Sevcik, Jan Stasek, Jan Malaska, Alena Sevcikova, Marketa Hanslianova, Zdenek Turek, Vladimir Cerny, Pavla Paterova
BIOMEDICAL PAPERS-OLOMOUC
(2016)
Article
Biotechnology & Applied Microbiology
Mu-Lu Wu, Martin Gengenbacher, Jade C. S. Chung, Swaine Lin Chen, Hans-Joachim Mollenkopf, Stefan H. E. Kaufmann, Thomas Dick
Article
Microbiology
Behnam Sayanjali, Gitte J. M. Christensen, Munir A. Al-Zeer, Hans -Joachim Mollenkopf, Thomas F. Meyer, Holger Bruggemann
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2016)
Article
Microbiology
Martin Gengenbacher, Natalie Nieuwenhuizen, Alexis Vogelzang, Haipeng Liu, Peggy Kaiser, Stefanie Schuerer, Doris Lazar, Ina Wagner, Hans-Joachim Mollenkopf, Stefan H. E. Kaufmann
Article
Microbiology
Jan Naujoks, Christoph Tabeling, Brian D. Dill, Christine Hoffmann, Andrew S. Brown, Mareike Kunze, Stefan Kempa, Andrea Peter, Hans-Joachim Mollenkopf, Anca Dorhoi, Olivia Kershaw, Achim D. Gruber, Leif E. Sander, Martin Witzenrath, Susanne Herold, Andreas Nerlich, Andreas C. Hocke, Ian van Driel, Norbert Suttorp, Sammy Bedoui, Hubert Hilbi, Matthias Trost, Bastian Opitz
Article
Gastroenterology & Hepatology
Philipp Schlaermann, Benjamin Toelle, Hilmar Berger, Sven C. Schmidt, Matthias Glanemann, Juergen Ordemann, Sina Bartfeld, Hans J. Mollenkopf, Thomas F. Meyer
Article
Cell Biology
Michael Sigal, Maria del Mar Reines, Stefanie Muellerke, Cornelius Fischer, Marta Kapalczynska, Hilmar Berger, Elvira R. M. Bakker, Hans-Joachim Mollenkopf, Michael E. Rothenberg, Bertram Wiedenmann, Sascha Sauer, Thomas F. Meyer
NATURE CELL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
January Weiner, David J. M. Lewis, Jeroen Maertzdorf, Hans-Joachim Mollenkopf, Caroline Bodinham, Kat Pizzoferro, Catherine Linley, Aldona Greenwood, Alberto Mantovani, Barbara Bottazzi, Philippe Denoel, Geert Leroux-Roels, Kent E. Kester, Ingileif Jonsdottir, Robert van den Berg, Stefan H. E. Kaufmann, Giuseppe Del Giudice
SCIENTIFIC REPORTS
(2019)
Article
Oncology
Hanna Leister, Maik Luu, Daniel Staudenraus, Aleksandra Lopez Krol, Hans-Joachim Mollenkopf, Arjun Sharma, Nils Schmerer, Leon N. Schulte, Wilhelm Bertrams, Bernd Schmeck, Markus Bosmann, Ulrich Steinhoff, Alexander Visekruna
Summary: The immunoproteasome has opposing roles in the tumor microenvironment, promoting colitis-associated carcinogenesis while exhibiting antitumorigenic activity in melanoma. This suggests that immunoproteasomes may have context-dependent pro- or antitumoral properties.
CANCER IMMUNOLOGY RESEARCH
(2021)
Article
Multidisciplinary Sciences
Stefanie Koster, Rajendra Kumar Gurumurthy, Naveen Kumar, Pon Ganish Prakash, Jayabhuvaneshwari Dhanraj, Sofia Bayer, Hilmar Berger, Shilpa Mary Kurian, Marina Drabkina, Hans-Joachim Mollenkopf, Christian Goosmann, Volker Brinkmann, Zachary Nagel, Mandy Mangler, Thomas F. Meyer, Cindrilla Chumduri
Summary: Using cervical organoid models, this study explores the dynamics of individual and coinfection with Human papillomavirus (HPV) and Chlamydia. The findings reveal that Chlamydia can interfere with HPV-induced mechanisms and potentially contribute to carcinogenesis.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, Research & Experimental
Anne-Sophie Fischer, Stefanie Muellerke, Alexander Arnold, Julian Heuberger, Hilmar Berger, Manqiang Lin, Hans-Joachim Mollenkopf, Jonas Wizenty, David Horst, Frank Tacke, Michael Sigal
Summary: The stomach corpus epithelium exhibits organized cellular differentiation controlled by RSPO3 during homeostasis and in response to chronic Helicobacter pylori infection, but its absence promotes pit cell differentiation. RSPO3 plays a crucial role in the recovery of corpus glands after injury, but in the context of chronic H. pylori infection, it leads to severe glandular hyperproliferation and the development of premalignant metaplasia.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biochemistry & Molecular Biology
Bingjian Ren, Manuela Schmid, Mattea Scheiner, Hans-Joachim Mollenkopf, Richard Lucius, Emanuel Heitlinger, Nishith Gupta
Summary: Successful asexual reproduction of intracellular pathogens relies on their ability to exploit host resources and evade antimicrobial defenses. In this study, two common apicomplexan parasites, Toxoplasma gondii and Eimeria falciformis, were used to investigate their interactions with mammalian cells, revealing distinct responses from individual host cells to different pathogens. The master transcription factor cFos was found to play a crucial role in the infection process, affecting the growth of both parasites in host cells. Overall, a network centered around cFos was identified, along with underlying signal cascades and a variety of nucleotide- and ion-binding proteins, which work together to help the mammalian cell adapt and facilitate parasite development.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)