Article
Biochemistry & Molecular Biology
Bhuvanasundar Renganathan, James P. Zewe, Yuan Cheng, Jean-Michel Paumier, Mark Kittisopikul, Karen M. Ridge, Puneet Opal, Vladimir I. Gelfand
Summary: Gigaxonin acts as an adaptor protein for E3 ubiquitin ligase and is essential for ubiquitination and degradation of intermediate filament (IF) proteins. Mutations in the GAN gene, which encodes gigaxonin, cause giant axonal neuropathy characterized by abnormal accumulation of IFs. This study shows that loss of gigaxonin inhibits IF transport along microtubules by the motor protein kinesin-1, leading to abnormal IF distribution.
Review
Biochemistry & Molecular Biology
Carlos Bueno-Beti, Angeliki Asimaki
Summary: Arrhythmogenic cardiomyopathy (ACM) is associated with ventricular arrhythmias and sudden cardiac death. Recent studies have shown that buccal cells behave similarly to the heart in terms of protein re-localization, which provides a potential alternative for diagnosis, risk stratification, and monitoring of pharmaceutical interventions for ACM.
Article
Cell Biology
Jinsook Ahn, Soyeon Jeong, So-Mi Kang, Inseong Jo, Bum-Joon Park, Nam-Chul Ha
Summary: This study examined the structural elements of intermediate filaments (IFs) and the role of coil 1a as a molecular regulator in the elongation of IF proteins, with a focus on the critical interactions between tetramers. It found that genetic mutations can result in weakened or augmented interactions between coil 1a and coil 2, affecting the structural transition in the central alpha-helical rod domain.
Article
Multidisciplinary Sciences
Aamir S. Mukadam, Lauren V. C. Miller, Annabel E. Smith, Marina Vaysburd, Siri A. Sakya, Sophie Sanford, Sophie Keeling, Benjamin J. Tuck, Taxiarchis Katsinelos, Chris Green, Lise Skov, Sanne S. Kaalund, Stian Foss, Keith Mayes, Kevin 'Connell, Mark Wing, Claire Knox, Jessica Banbury, Edward Avezov, James B. Rowe, Michel Goedert, Jan Terje Andersen, Leo C. James, William A. McEwan
Summary: Passively transferred antibodies can target and protect against tau pathology in neurodegenerative diseases. The cytosolic Ab receptor and E3 ligase TRIM21 have been identified as potential mechanisms of antibody protection against tau aggregation.
Review
Oncology
Georgios Pampalakis, Georgios Angelis, Eleni Zingkou, Kostas Vekrellis, Georgia Sotiropoulou
Summary: ALS is a fatal disease involving degeneration of motor neurons. Multiple causative genes have been identified, indicating diverse pathways leading to ALS. Chemical entities are a promising choice to delay ALS progression, but have shown no efficacy in clinical trials. Exploiting the chemical entity-genotype correlation could guide patient stratification for treatment.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Microbiology
Marko Nedeljkovic, Mark A. B. Kreutzberger, Sandra Postel, Daniel Bonsor, Yingying Xing, Neil Jacob, William J. Schuler, Edward H. Egelman, Eric J. Sundberg
Summary: Bacterial flagellar filaments consist of inner and outer domains, with outer domains playing a crucial role in motility. The ridged filament structure, characterized by dimerization of outer domains, enables bacteria to swim in viscous environments. This filament organization is widespread among diverse bacterial species.
Article
Immunology
Daniel de Oliveira Patricio, Greicy Brisa Malaquias Dias, Lucilene Wildner Granella, Ben Trigg, Helena Claire Teague, Dina Bittencourt, Andre Bafica, Alfeu Zanotto-Filho, Brian Ferguson, Daniel Santos Mansur
Summary: DNA-PKcs plays a role in restricting the spread of Zika virus infection in human cells by influencing the transcription of interferons and related genes. This mechanism is independent of DNA breaks.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Plant Sciences
Na Mu, Yafei Li, Sanhe Li, Wenqing Shi, Yi Shen, Han Yang, Fanfan Zhang, Ding Tang, Guijie Du, Aiqing You, Zhukuan Cheng
Summary: In this study, using physiological, cytological, and genetic approaches, it was found that MUS81 in rice functions in atypical recombination intermediate resolution rather than crossover designation. The total chiasma numbers and HEI10 foci (the sites of interference-sensitive crossovers) in mus81 mutants were similar to wild-type. Disruption of MUS81 in msh5 or msh4 msh5 background did not further decrease chiasmata frequency, indicating that rice MUS81 did not play a role in crossover designation. However, chromosome fragments and bridges were frequently observed in mus81 zep1 and mus81 fancm, suggesting that MUS81 may resolve atypical recombination intermediates.
Article
Multidisciplinary Sciences
Hannah Wilkinson, Hugh Leonard, Daxin Chen, Toby Lawrence, Michael Robson, Pieter Goossens, John H. McVey, Anthony Dorling
Summary: Research has shown that in chronic inflammation, the PAR-1 signal is crucial for granuloma formation, with this signal acting on macrophages through the activation of Thrombin. The results of the experiment indicate that Thrombin signaling significantly enhances macrophage sensitivity to IFN-γ, leading to enhanced cell polarization.
Article
Microbiology
Rosa Milagros Corrales, Slavica Vaselek, Rachel Neish, Laurence Berry, Camille D. Brunet, Lucien Crobu, Nada Kuk, Julio Mateos-Langerak, Derrick R. Robinson, Petr Volf, Jeremy C. Mottram, Yvon Sterkers, Patrick Bastien
Summary: The deletion of the sole kinesin among FAZ proteins disrupts cell morphogenesis, FP organization, and cell division in Leishmania parasites. Proliferation in the insect vector and mammalian host is reduced in parasites lacking the kinesin FAZ7B, highlighting its importance in the successful life cycle and pathogenicity of the parasite.
Article
Biochemistry & Molecular Biology
Marko Nedeljkovic, Sandra Postel, Brian G. Pierce, Eric J. Sundberg
Summary: The study identified the D1 domain of FliD as crucial for functional flagella formation, with the head region showing good tolerance to sequences from different bacterial species. Additionally, head domains can be replaced by small peptides or larger head domains from different species and still produce functional flagella.
Article
Biophysics
Ayana Yamagishi, Mei Mizusawa, Koki Uchida, Masumi Iijima, Shun'ichi Kuroda, Kyoko Fukazawa, Kazuhiko Ishihara, Chikashi Nakamura
Summary: This study developed a mechanical detection method using nanoneedles to measure protein-protein interactions in living cells and discovered the interaction between IF vimentin and transcription-related proteins.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Cell Biology
Kiran Pandey, Xiao-Wen Yu, Adam Steinmetz, Cristina M. Alberini
Summary: An increase in autophagy following learning is essential for long-term memory formation, but not necessary for the increase in protein synthesis. The upregulation of autophagy proteins is due to active translation of their mRNA rather than changes in total mRNA levels.
Article
Cell Biology
Hang Xu, Shuai Jiang, Chao Yu, Zihao Yuan, Li Sun
Summary: This study revealed a previously unrecognized bi-directional regulation mode of GSDME-mediated pyroptosis, and a functional difference between teleost GSDMEa and GSDMEb in the immune defense against bacterial infection.
CELL DEATH & DISEASE
(2022)
Article
Polymer Science
Claudio Tosto, Jacopo Tirillo, Fabrizio Sarasini, Claudia Sergi, Gianluca Cicala
Summary: Metal 3D-printed parts have important applications in various industries, but traditional manufacturing techniques are expensive. This study combines fused deposition modeling (FDM) with debinding-sintering processes to reduce costs. By optimizing printing parameters, the material density and mechanical performance of sintered stainless steel parts were improved.
Article
Oncology
Xinyu Wu, Fei Chen, Aysegul Sahin, Constance Albarracin, Zhiheng Pei, Xuanyi Zou, Baljit Singh, Ruliang Xu, Garrett Daniels, Yirong Li, Jianjun Wei, Marvin Blake, Robert J. Schneider, Pamela Cowin, Peng Lee
BREAST CANCER RESEARCH AND TREATMENT
(2011)
Editorial Material
Biochemistry & Molecular Biology
Pamela Cowin
CELL COMMUNICATION AND ADHESION
(2013)
Editorial Material
Biochemistry & Molecular Biology
Pamela Cowin
CELL COMMUNICATION AND ADHESION
(2013)
Review
Biochemistry & Molecular Biology
Julia Simundza, Pamela Cowin
CELL COMMUNICATION AND ADHESION
(2013)
Editorial Material
Biochemistry & Molecular Biology
Mario Delmar, Kathleen Green, Pamela Cowin
CELL COMMUNICATION AND ADHESION
(2014)
Editorial Material
Biochemistry & Molecular Biology
Pamela Cowin, Werner Franke
CELL COMMUNICATION AND ADHESION
(2014)
Editorial Material
Biochemistry & Molecular Biology
Pamela Cowin
CELL COMMUNICATION AND ADHESION
(2014)
Article
Oncology
Anupama Chandramouli, Julia Simundza, Alicia Pinderhughes, Pamela Cowin
JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA
(2011)
Article
Multidisciplinary Sciences
Roman Anton, Sujash S. Chatterjee, Julia Simundza, Pamela Cowin, Ramanuj DasGupta
Article
Multidisciplinary Sciences
Anupama Chandramouli, Sarah J. Hatsell, Alicia Pinderhughes, Lisa Koetz, Pamela Cowin
Article
Biochemical Research Methods
Atish Mukherjee, Selma M. Soyal, Jie Li, Yan Ying, Maria M. Szwarc, Bin He, Ramakrishna Kommagani, Myles C. Hodgson, Minoti Hiremath, Pamela Cowin, John P. Lydon
TRANSGENIC RESEARCH
(2011)
Review
Cell Biology
Elena Spina, Pamela Cowin
Summary: Embryonic mammary gland development involves multiple steps, including the formation of mammary placodes and mammary buds, as well as the development of ductal trees. Studies have identified factors and pathways that influence these steps, and there is a deeper understanding of the characteristics of mammary stem cells and progenitor cells.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Elena Spina, Julia Simundza, Angela Incassati, Anupama Chandramouli, Matthias C. Kugler, Ziyan Lin, Alireza Khodadadi-Jamayran, Christine J. Watson, Pamela Cowin
Summary: Gpr125 is a specific marker for mammary stem cells and basal progenitors. It plays a crucial role in tumor formation and prognosis. Through lineage tracing experiments, the researchers demonstrate that Gpr125 is a highly specific marker for mammary stem cells and basal mammary progenitors. High expression of Gpr125 can predict early tumor onset and poor prognosis in basal breast cancer.
NATURE COMMUNICATIONS
(2022)
Article
Oncology
Anupama Chandramouli, Julia Simundza, Alicia Pinderhughes, Minoti Hiremath, Gustavo Droguett, David Frendewey, Pamela Cowin
BREAST CANCER RESEARCH
(2013)
Review
Oncology
Angela Incassati, Anupama Chandramouli, Rachel Eelkema, Pamela Cowin
BREAST CANCER RESEARCH
(2010)