Article
Oncology
Tibor Hajdu, Patrik Kovacs, Emese Zsigrai, Roland Takacs, Judit Vago, Sinyoung Cho, Laszlo Sasi-Szabo, Daniel Becsky, Aniko Keller-Pinter, Gabriella Emri, Kalman Racz, Dora Reglodi, Roza Zakany, Tamas Juhasz
Summary: The study confirms the expression of PACAP receptors in melanocytes and melanoma cells, demonstrating that PACAP can inhibit proliferation and migration of melanoma cells, making it a potential target in melanoma progression.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Paola Matarrese, Sonia Maccari, Barbara Ascione, Rosa Vona, Vanessa Vezzi, Tonino Stati, Maria Cristina Gro, Giuseppe Marano, Caterina Ambrosio, Paola Molinari
Summary: This study found that beta-AR stimulation affects the proliferative activity of B16F10 cells by regulating cAMP levels. Additionally, alpha 2-AR agonists can inhibit cell proliferation, which is disrupted by the inhibitory effect of beta-AR agonists on alpha 2-AR. These findings suggest that the crosstalk between beta-AR and alpha 2-AR signaling pathways plays an important role in regulating melanoma proliferation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Mauricio Reyna-Jeldes, Erwin De la Fuente-Ortega, Daniela Cerda, Erandi Velazquez-Miranda, Katherine Pinto, Francisco G. Vazquez-Cuevas, Claudio Coddou
Summary: The study investigated the role of purinergic signaling in gastric cancer, revealing that the expression patterns of P2Y2R and P2X4R in GC cells are associated with cell proliferation, migration, and epithelial-mesenchymal transition (EMT).
Article
Biochemistry & Molecular Biology
Kun Zhang, Ruoxi Tian, Wancong Zhang, Yishuai Li, Ning Zeng, Yan Liang, Shijie Tang
Summary: The glycolytic enzyme ENO1 is overexpressed in skin cutaneous melanoma (SKCM) and promotes invasion and proliferation of tumor cells, as well as playing an important role in tumor cell glycolysis. Therefore, ENO1 represents a potential therapeutic target for SKCM treatment.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Anatomy & Morphology
Yanfei Li, Xia Yun, Jiaojiao Li, Meilin Bai
Summary: In this study, the researchers found that the expression levels of IGHG1, ZEB1, and CSTF2T were all high in melanoma cells. ZEB1 directly binds to the IGHG1 promoter, promoting melanoma cell proliferation and invasion. CSTF2T directly binds to ZEB1 mRNA, and silencing CSTF2T inhibits ZEB1 expression, thereby suppressing melanoma cell proliferation and invasion.
Article
Biochemistry & Molecular Biology
Chen-Wei Chiang, Yun-Shih Lin, Fu-Ling Chang, Tsai-Yu Lin, Keng-Chang Tsai, Wei-Chun HuangFu, Yu-Ching Lee
Summary: Studies have shown that high expression of EphA4 in gastric cancer tissues is associated with unfavorable clinical pathological characteristics. In this study, the generated scFv S3 was found to bind to endogenous EphA4 in gastric cancer cells, inhibiting the growth and migration of cancer cells. The administration of scFv S3 also resulted in decreased signaling molecules and improved therapeutic effects in a xenograft model. Furthermore, scFv S3 was able to degrade EphA4 molecules in tumor tissues and generate essential ionic bonding with the target protein.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Akash Gupta, Jeremias G. Galletti, Zhiyuan Yu, Kevin Burgess, Cintia S. de Paiva
Summary: Neurotrophins, including NGF, BDNF, NT-3, and NT-4, play important roles in promoting neuron differentiation, development, and survival. NGF has been approved for the treatment of neurotrophic keratitis, while the other neurotrophins are less studied in ocular surface diseases. Neurotrophin analogs with panTrk activity and TrkC selectivity show promise as novel drugs for treating dry eye disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Shaomin Shi, Chongyang Li, Yanli Zhang, Chaowei Deng, Mengqin Tan, Guangzhao Pan, Juan Du, Yacong Ji, Qian Li, Hanghua Liang, Wei Liu, Leiyang Guo, Gaichao Zhao, Yaling Liu, Hongjuan Cui
Summary: The active ingredient Lycorine hydrochloride sourced from the medicinal herb Lycoris radiata has been shown to possess tumor suppression activity in various human cancers. In melanoma, an increased expression of p21(Cip1/WAF1) was found in tumor tissues and correlated with tumor invasion depth. Functional experiments demonstrated that LH effectively inhibited the proliferation, migration, and invasion ability of melanoma cells by inducing S phase cell cycle arrest and regulating epithelial-mesenchymal transition (EMT).
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Review
Oncology
Gloria Angelina Herrera-Quiterio, Sergio Encarnacion-Guevara
Summary: TMEM proteins play important roles in cancer progression, including cell proliferation, invasion, migration, and response to chemotherapy. However, the exact mechanisms and physiological functions of these proteins are still not well understood.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Quan Lin, Yan Jia, Duo Zhang, Hongjuan Jin
Summary: The study reveals that NCK1-AS1 is overexpressed in melanoma cell lines, and its knockdown inhibits the proliferation and migration of melanoma cells. Additionally, miR-526b-5p binds to NCK1-AS1 in melanoma, with ADAM15 validated as its downstream target.
CANCER CELL INTERNATIONAL
(2021)
Article
Cell Biology
Yazhen Xu, Bingyu Guo, Xiaoyan Liu, Kai Tao
Summary: miR-34a inhibits melanoma growth by targeting ZEB1, suggesting a potential novel target for melanoma treatment.
Article
Oncology
Xinxin Zhou, Jiayuan He, Qingyuan Wang, Teng Ma
Summary: The study revealed that miR-128-3p acts as a tumor suppressor in malignant melanoma cells by inhibiting proliferation, migration, invasion, and inducing apoptosis. The oncogene neurotrophin receptor 3 (NTRK3) was found to be up-regulated in MM cells, enhancing their malignant behaviors. The interaction between miR-128-3p and NTRK3 genes modulate the malignant behavior of MM, suggesting potential new therapeutic targets.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Huan Ma, Siyu Jiang, Yinan Yuan, Ji Li, Yizhuo Li, Yanping Lv, Tengjiao Du, Jingqian Guan, Xizi Jiang, Lei Tian, Qianqian Zheng, Lianhe Yang, Qingchang Li
Summary: RUNX1, a member of the RUNX family, is involved in the regulation of non-small cell lung cancer. It promotes cell proliferation and migration via the mTOR pathway and may serve as a potential therapeutic target for NSCLC.
Article
Biochemistry & Molecular Biology
Maxim L. Bychkov, Artem Kirichenko, Irina N. Mikhaylova, Alexander S. Paramonov, Evgeny Yastremsky, Mikhail P. Kirpichnikov, Mikhail A. Shulepko, Ekaterina N. Lyukmanova
Summary: Metastatic melanoma cells release extracellular vesicles (EVs) that can influence the morphology and homeostasis of normal keratinocytes, promoting tumor growth and migration. Additionally, EVs can inhibit the secretion of certain factors by keratinocytes, resulting in worse survival prognosis.
Review
Biochemistry & Molecular Biology
Giovannamaria Petrocelli, Luca Pampanella, Provvidenza M. Abruzzo, Carlo Ventura, Silvia Canaider, Federica Facchin
Summary: This article reviews the role of endogenous opioids in stem cell biology, particularly their ability to regulate stem cell proliferation, stress response, and differentiation into various lineages. This has important implications for understanding the characteristics of stem cells and their application in regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Dermatology
Jiahua Li, Erdong Wei, Amin Reisinger, Lars Einar French, Benjamin M. Clanner-Engelshofen, Markus Reinholz
Summary: This study aims to evaluate the effectiveness of different anti-Demodex strategies and provide objective observations based on quantified Demodex mite data. The results show that ivermectin, ivermectin-metronidazole, and tea tree oil are promising interventions against Demodex. In addition to traditional pharmacotherapy, light therapies, especially IPL, and skin cleansing can also be considered effective methods for controlling Demodex infestation.
Review
Dermatology
Pia-Charlotte Stadler, Anna Oschmann, Katrin Kerl-French, Julia-Tatjana Maul, Eva Maria Oppel, Barbara Meier-Schiesser, Lars Einar French
Summary: Acute generalized exanthematous pustulosis (AGEP) is a potentially severe adverse cutaneous drug reaction that manifests as numerous sterile subcorneal pustules on erythematous skin. It can be associated with organ manifestations and typically resolves rapidly upon removal of the culprit drug and adequate steroid therapy. Recent experimental data suggest an early role of drug-induced innate immune activation and innate cytokines in the pathogenesis of AGEP.
Review
Dermatology
Julia-Tatjana Maul, Carole Guillet, Anna Oschmann, Lara Valeska Maul, Barbara Meier-Schiesser, Pia-Charlotte Stadler, Lars E. French, Katrin Kerl
Summary: Cutaneous lichenoid drug eruptions (LDE) are rare adverse drug reactions (ADR) that resemble lichen planus and are mainly induced by checkpoint inhibitors (CKI), tyrosine kinase inhibitors (TKI), and anti-TNF-alpha monoclonal antibodies. Diagnosis and treatment of LDE are challenging, and discontinuation of the culprit drug is not always necessary.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Article
Dermatology
Jonathan I. I. Silverberg, Lars E. E. French, Richard B. B. Warren, Bruce Strober, Kim Kjoller, Morten O. A. Sommer, Philippe Andres, Jakob Felding, Anne Weiss, Deniz Tutkunkardas, Tine Skak-Nielsen, Emma Guttman
Summary: This study examined the PDE4 enzymatic activity and anti-inflammatory effects of orismilast in vitro, ex vivo, and in vivo. The results showed that orismilast selectively and potently inhibited PDE4 and demonstrated broad-spectrum anti-inflammatory activity. These findings support the clinical development of oral orismilast as a novel treatment option for chronic inflammatory skin diseases.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Article
Dermatology
Richard B. B. Warren, Bruce Strober, Jonathan I. I. Silverberg, Emma Guttman, Philippe Andres, Jakob Felding, Deniz Tutkunkardas, Kim Kjoller, Morten O. A. Sommer, Lars E. E. French
Summary: This study aimed to examine the efficacy and safety of orismilast for psoriasis treatment. The phase 2a trial found that orismilast significantly improved the condition of patients with psoriasis. The phase 1 trial tested new formulations to minimize gastrointestinal adverse reactions.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Article
Education, Scientific Disciplines
Matthias D. D. Kaufmann, Theresa Steeb, Anja Wessely, Marion Meyerolbersleben, Lars E. E. French, Carola Berking, Markus V. V. Heppt
Summary: The use of immunotherapies has greatly expanded treatment options and improved prognosis for advanced cancer patients in the past decade. We have developed a virtual teaching module called eImmunonkologie, which is the first interdisciplinary virtual course on immuno-oncology for medical students in German-speaking countries.
MEDICAL SCIENCE EDUCATOR
(2023)
Article
Biology
Roberta Lotti, Claudio Giacinto Atene, Emma Dorotea Zanfi, Matteo Bertesi, Carlo Pincelli, Tommaso Zanocco-Marani
Summary: Researchers have developed new active models to mimic the main forms of Pemphigus, including PV and mucocutaneous Pemphigus. Immunized animals and animals that received splenocytes from the immunized donors produced high concentrations of circulating antibodies against specific antigens, but the effectiveness of treatment with corticosteroids was limited.
Article
Immunology
Theresa Ruf, Anna Leonhardt, David Anz, Wolfgang G. Kunz, Natascha Roehrle, Paul Foerster, Dirk Tomsitz, Lars Einar French, Marlene Seegraeber, Lucie Heinzerling
Summary: Tebentafusp has been approved as a new treatment for HLA-A*02:01-positive uveal melanoma patients and can improve their survival. However, it may induce cytokine-release syndrome and tumor lysis syndrome, which are potential life-threatening conditions. It is important for healthcare professionals to be aware of these adverse events and promptly treat them.
Article
Oncology
Marika Quadri, Natascia Tiso, Francesco Musmeci, Maria I. I. Morasso, Stephen R. R. Brooks, Luca Reggiani Bonetti, Rossana Panini, Roberta Lotti, Alessandra Marconi, Carlo Pincelli, Elisabetta Palazzo
Summary: This study found differential expression of CD271 in low-risk and high-risk cSCC tumors, and demonstrated that CD271 activity can decrease the proliferation and invasiveness of cSCC, while promoting tumor cell differentiation. CD271 also inhibits the metastatic process of cSCC and increases sensitivity to therapy. Therefore, CD271 may be a promising target for future pharmaceutical development.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Immunology
Roberta Lotti, Jennifer E. Hundt, Ralf J. Ludwig, Brydon Bennett, Antonino Amato, Alessandra Marconi, Carlo Pincelli
Summary: Pemphigus is a life-threatening autoimmune bullous disease that affects the skin and mucous membranes. Current therapies aim to suppress the immune system, but PC111, a fully human monoclonal antibody targeting Fas ligand, has shown promising results in reducing blister formation. In vitro and in vivo experiments have confirmed the mechanism of action and efficacy of PC111 in preventing acantholysis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Review
Dermatology
Takashi K. Satoh, Matthias Munoz Neulinger, Pia-Charlotte Stadler, Rui Aoki, Lars E. French
Summary: Immune checkpoint inhibitors (ICIs) have revolutionized cancer treatment, but they can also lead to immune-related adverse events (irAEs), including severe cutaneous reactions such as Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN). This study analyzed 95 cases of ICI-induced SJS/TEN and found that PD-1 inhibitors were most commonly associated with these reactions. SJS/TEN typically occurred within the first 4 weeks of treatment, and corticosteroid monotherapy was the most frequently chosen treatment approach. The overall mortality rate of ICI-induced SJS/TEN was high.
JOURNAL OF DERMATOLOGY
(2023)