Article
Biotechnology & Applied Microbiology
Haruna Hori, Ayaka Kotani, Junya Abe, Shuji Matsuguchi, Yohei Hirai
Summary: The expression and processing of filaggrin in the skin epidermis is closely related to keratinocyte cornification. Extracellular EPM inhibits the expression of Pro-FLG and reduces the production of NMF in HaCaT keratinocytes.
Article
Dermatology
Gabriela Blanchard, Christine Pich, Daniel Hohl
Summary: Primary cilium (PC) is a microtubule-based organelle that plays an important role in development and tissue homeostasis. Ciliopathies are a group of human diseases characterized by disordered cilium. In this study, it was found that HaCaT cells, a model for studying epidermal homeostasis, can efficiently develop cilia. The detailed progression of ciliation in HaCaT cells can serve as a basis for further ciliary studies in this cell line.
EXPERIMENTAL DERMATOLOGY
(2022)
Review
Cell Biology
Hanxiao Yin, Mingzheng Hu, Dengwen Li
Summary: The epidermis is a barrier in the outermost layer of the skin that protects against pathogens. Basal keratinocytes, acting as stem cells, are crucial for epidermal development and skin damage recovery. Understanding the molecular mechanism of basal keratinocytes in epidermal development and stratification is important for preventing and treating skin lesions.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Can Li, Yuying Zhu, Weiwei Liu, Wendie Xiang, Sijun He, Toshihiko Hayashi, Kazunori Mizuno, Shunji Hattori, Hitomi Fujisaki, Takashi Ikejima
Summary: This study focuses on the involvement of mitochondrial disorders in UVB-induced skin damages. UVB irradiation increases the amount of dysfunctional mitochondria in HaCaT cells, leading to decreased ATP production, mitochondrial membrane potential collapse, and apoptosis. The impairment of PINK1/parkin-mediated mitophagy contributes to UVB-induced cell damages by activating cGAS-STING pathway and promoting caspase-3-mediated apoptosis. Restoring mitophagy may be a potential strategy to protect skin from UVB damages.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Multidisciplinary Sciences
Chujiao Lin, Qiyuan Yang, Dongsheng Guo, Jun Xie, Yeon-Suk Yang, Sachin Chaugule, Ngoc DeSouza, Won-Taek Oh, Rui Li, Zhihao Chen, Aijaz A. John, Qiang Qiu, Lihua Julie Zhu, Matthew B. Greenblatt, Sankar Ghosh, Shaoguang Li, Guangping Gao, Cole Haynes, Charles P. Emerson, Jae-Hyuck Shim
Summary: This study demonstrates that mitochondrial oxidative phosphorylation is the major energy source for osteogenesis of skeletal progenitors. Deletion of ECSIT hinders bone formation and regeneration, resulting in skeletal deformity and defects in the bone marrow niche.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Michael L. Samulevich, Rambon Shamilov, Brian J. Aneskievich
Summary: Intrinsically disordered proteins (IDPs) in keratinocytes, especially those associated with the cornified envelope, demonstrate high levels of intrinsic disorder and potential for liquid-liquid phase separation (LLPS).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Nobutomo Ikarashi, Miho Kaneko, Daigo Wakana, Yui Shinozaki, Keito Tabata, Yui Nishinaka, Ryotaro Yoshida, Tomofumi Watanabe, Nobuyuki Wakui, Risako Kon, Hiroyasu Sakai, Junzo Kamei, Tomoo Hosoe
Summary: The study found that Chimpi extract can increase the expression levels of AQP3 water channel protein in keratinocytes and promote cell migration. This suggests that Chimpi extract may have potential therapeutic effects for the management of dry skin.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biochemistry & Molecular Biology
In Ah Bae, Jae Won Ha, Joon Yong Choi, Yong Chool Boo
Summary: The antioxidant effect of Korean propolis on oxidative stress in human epidermal keratinocytes exposed to air particulate matter was studied. The hydrophilic fraction of propolis showed potential in inhibiting oxidative stress induced by particulate matter and reducing toxicity.
Article
Biochemistry & Molecular Biology
Yi Li Wong, Toru Okubo, Eiko Uno, Kazuma Suda, Tsuyoshi Ishii
Summary: CD99 is a glycoprotein primarily expressed in immune cells. Its function in the skin remains unclear. This study investigated the role of CD99 in the skin using normal human epidermal keratinocytes (NHEK). The results suggest that CD99 is involved in maintaining skin homeostasis and initiating early differentiation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Dermatology
Jang-Hee Oh, Woojune Hur, Na Li, Seong Jin Jo
Summary: EGFRIs suppress lipogenesis and HA metabolism, which may contribute to adverse dermatological effects, including barrier function impairment in cancer patients treated with EGFRIs.
EXPERIMENTAL DERMATOLOGY
(2022)
Article
Plant Sciences
Mawalle Kankanamge Hasitha Madhawa Dias, Dissanayaka Mudiyanselage Dinesh Madusanka, Eui Jeong Han, Hyun-Soo Kim, You-Jin Jeon, Youngheun Jee, Kil-Nam Kim, Kyounghoon Lee, Ilekuttige Priyan Shanura Fernando, Ginnae Ahn
Summary: The study found that the ethanol extract from Sargassum horneri has protective effects against fine dust-induced inflammatory responses and impaired skin hydration in keratinocytes. This could be attributed to its suppression of inflammatory mediators and regulation of skin barrier function.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Mariya Vildanova, Polina Vishnyakova, Aleena Saidova, Victoria Konduktorova, Galina Onishchenko, Elena Smirnova
Summary: Gibberellic acid (GA) induces mild ER stress and differentiation in human cells, especially carcinoma A431 cells, potentially reducing malignancy and tumorigenic potential.
Article
Biochemistry & Molecular Biology
Tetyana Chumak, Diana Tothova, Iva Filova, Zbynek Bures, Jiri Popelar, Gabriela Pavlinkova, Josef Syka
Summary: By studying the responses of IC neurons in Tg mice to different sound stimuli, it was found that Isl1 overexpression led to increased inhibition, expanded dynamic range, and other functional changes, accompanied by a decrease in specific neuron numbers and an increase in protein expression levels in the IC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Wieslawa Lesniak, Anastasiia Bohush, Malgorzata Maksymowicz, Cezary Piwowarczyk, Natalia Katarzyna Karolak, Ewelina Jurewicz, Anna Filipek
Summary: In this study, researchers found that CacyBP/SIP protein is present in the epidermis and plays a role in epidermal differentiation and response to viral infection.
Article
Dermatology
I Cosgarea, A. T. McConnell, T. Ewen, D. Tang, D. S. Hill, M. Anagnostou, M. Elias, R. A. Ellis, A. Murray, L. C. Spender, P. Giglio, M. Gagliardi, A. Greenwood, M. Piacentini, G. J. Inman, G. M. Fimia, M. Corazzari, J. L. Armstrong, P. E. Lovat
Summary: The loss of AMBRA1 in the peritumoral epidermis is associated with an increased risk of metastasis in early-stage melanoma patients, possibly due to disruption of epidermal integrity. TGF-beta 2 signaling may play a role in this process by downregulating AMBRA1 and other epidermal markers, leading to decreased epidermal integrity and tumor ulceration.
BRITISH JOURNAL OF DERMATOLOGY
(2022)