Article
Virology
Han Lei, Shuangqin Li, Xin Lu, Yi Ren
Summary: By utilizing a Saccharomyces cerevisiae surface display system, a potential vaccine candidate against White spot syndrome virus (WSSV) was developed and shown to induce significant immune-related enzyme activities in shrimp when orally administered. The oral vaccine conferred 100% protection and reduced viral load in shrimp, highlighting its potential as an efficient control strategy against WSSV infection in shrimp aquaculture.
Article
Microbiology
Siyuan Zhang, Xumei Sun
Summary: The gut microbiota plays a key role in the immunity of shrimp, and changes in the core microbiota are observed during viral infection. Metabolomic analysis reveals nine significantly upregulated metabolites with antiviral potential after viral infection. These findings highlight the importance of the gut microbiota and metabolome in maintaining immune homeostasis and antiviral immunity in shrimp.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Min-Jeong Kim, Su-Hyun Kim, Jong-Oh Kim, Taek-Kyun Lee, In-Kwon Jang, Tae-Jin Choi
Summary: VP28 protein expressed in Chlorella vulgaris can serve as an oral shrimp vaccine against white spot syndrome virus (WSSV), significantly reducing the mortality rate and upregulating the expression of shrimp defense genes.
Article
Agriculture, Dairy & Animal Science
Fuxuan Wang, Shihao Li, Fuhua Li
Summary: The lymphoid organ is a vital part of the shrimp immune system, exhibiting different functions in response to different pathogens, providing new insights into the immune functions of the lymphoid organ in shrimp.
Article
Engineering, Marine
Ziwei Wu, Ka Hou Chu, Ka Yan Ma
Summary: We examined the transcriptomic changes in three immune tissues (gills, hepatopancreas, and hemocytes) of Penaeus vannamei infected with white spot syndrome virus (WSSV), Vibrio parahaemolyticus acute hepatopancreatic necrosis disease (VPAHPND), and decapod iridovirus 1 (DIV1). The results showed tissue-specific differences in the immune responses of shrimp, with differentially expressed genes (DEGs) in gills mainly related to environmental information processing and cellular processes, DEGs in hemocytes mostly involved in cellular processes, and DEGs in hepatopancreas primarily associated with metabolism. These findings provide new insights into the immune responses of Penaeus vannamei and contribute to sustainable shrimp farming.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2023)
Article
Immunology
Yashdeep Phanse, Supraja Puttamreddy, Duan Loy, Julia Vela Ramirez, Kathleen A. Ross, Ignacio Alvarez-Castro, Mark Mogler, Scott Broderick, Krishna Rajan, Balaji Narasimhan, Lyric C. Bartholomay
Summary: In the past 15 years, crustacean fisheries have suffered significant economic losses due to viral diseases. No effective measures are available to prevent or control these outbreaks. However, a double-stranded RNA-based nanovaccine shows promise for controlling infectious diseases in aquaculture.
Article
Fisheries
Wang-Jing Liu, Yun-Shiang Chang, Kou-Chun Tseng, Meng-Hua Yu
Summary: White spot syndrome virus (WSSV) is a prominent pathogen in shrimp farming worldwide. However, there is currently no effective treatment for this virus. In this study, the antiviral properties of bovine lactoferrin (bLF) against WSSV were investigated, and it was found that bLF treatment significantly reduced shrimp mortalities caused by WSSV infection. The ability of bLF to bind to both host cells and WSSV virions suggests its potential as an anti-WSSV agent in shrimp culture, and the identified interaction proteins may play a role in WSSV infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Carlos R. Romo Quinonez, Pindaro Alvarez-Ruiz, Claudio H. Mejia-Ruiz, Nina Bogdanchikova, Alexey Pestryakov, Carina Gamez-Jimenez, Wenceslao Valenzuela-Quinonez, Magnolia Montoya-Mejia, Eusebio Nava Perez
Summary: The application of silver nanoparticles as feed additives for shrimp has shown potential in preventing WSSV infection. However, it negatively affects the transcriptional responses of immune-related genes.
Article
Fisheries
Jie Ren, Wenyang Lin, Hong Shi, Yiwen Jian, Lingwei Ruan
Summary: The Hippo signaling pathway, including its core molecule Yorkie, plays important roles in organ size control, development, tumorigenesis, and immunity. In this study, a Yorkie homolog named LvYKI was cloned and characterized from Litopenaeus vannamei. LvYKI showed different regulatory mechanisms in different tissues and was involved in white spot syndrome virus (WSSV) infection. Knocking down of LvYKI inhibited the transcription of WSSV genes and delayed shrimp mortality, while increasing hemocyte apoptosis. Moreover, LvYKI was found to interact with Lv beta-catenin, linking the Wnt and Hippo signaling pathways in innate immunity. This study provides insights into the role of LvYKI in the interaction between shrimp and virus, enhancing our understanding of the molecular mechanism in innate immunity.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Beder Ramirez, Mervin Guevara, Ruben Alfaro, Vanessa Montoya, Hung N. Mai, Maria Serna, Arun K. Dhar, Luis Fernando Aranguren
Summary: The study conducted in wild shrimp near Tumbes, Peru, revealed the presence of various shrimp pathogens, with a higher prevalence of Baculovirus penaei and white spot syndrome virus. Wild shrimp may serve as potential carriers for diseases in nearby shrimp farming areas.
AQUACULTURE RESEARCH
(2021)
Article
Fisheries
Vernita Priya, Sudhakaran Raja
Summary: Shrimp aquaculture is booming because of high demand in developed countries, but the white spot syndrome virus (WSSV) is causing massive mortality in shrimp population. Nanotechnology provides advantages in targeted drug delivery, altering charge of nanoparticles, and minimizing adverse effects. This review discusses antiviral activity of CS/TPP-coated nanoparticles encapsulating WSSV VP28, stimulation of innate immunity using polyamine-coated carbon quantum dots, and investigation of silver nanoparticles in inhibiting viral activity for effective cure of white spot syndrome.
AQUACULTURE INTERNATIONAL
(2023)
Article
Multidisciplinary Sciences
Tze Chiew Christie Soo, Subha Bhassu
Summary: In recent years, shrimp aquaculture has become the major source of global shrimp production. However, shrimp production has been hindered by various shrimp diseases. This study compared the genetic factors and evolutionary divergence between two important shrimp species, Macrobrachium rosenbergii and Penaeus monodon, and successfully identified important selection forces.
Review
Fisheries
Carlos Fajardo, Gonzalo Martinez-Rodriguez, Benjamin Costas, Juan Miguel Mancera, Sergio Fernandez-Boo, Hectorina Rodulfo, Marcos De Donato
Summary: Researchers have conducted meta-analysis on the crustacean immune system using transcriptomic analysis and databases, revealing the impact of pathogens, abiotic stressors, dietary factors, and beneficial microbes on genes and metabolic pathways. Efforts are needed to develop genomic tools for commercial applications aiming to improve culture conditions and develop more disease-resistant strains.
REVIEWS IN AQUACULTURE
(2022)
Article
Multidisciplinary Sciences
Mostafa Rakhshaninejad, Liping Zheng, Hans Nauwynck
Summary: This study found that Penaeus vannamei shrimp infected with White spot syndrome virus (WSSV) use behavioral fever to increase their body temperature, inhibit virus replication, and prevent infection and mortality.
SCIENTIFIC REPORTS
(2023)
Article
Fisheries
Chao Zhao, Chao Peng, Pengfei Wang, Bo Zhang, Lulu Yan, Chun-Lin Wang, Lihua Qiu
Summary: In this study, a novel TRIM37 homolog called PmTRIM37 was identified, which plays important roles in regulating innate immune processes, particularly antiviral and proinflammatory cytokine responses. PmTRIM37 is highly expressed in immune-related tissues and its expression is up-regulated after white spot syndrome virus (WSSV) infection. Knockdown of PmTRIM37 promotes WSSV replication and VP28 expression, suggesting its negative role in WSSV infection. Furthermore, PmTRIM37 positively regulates the NF-κB pathway and Antimicrobial peptides (AMP) expression during WSSV infection.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
A. Panigrahi, R. R. Das, M. R. Sivakumar, A. Saravanan, C. Saranya, N. S. Sudheer, K. P. Kumaraguru Vasagam, P. Mahalakshmi, S. Kannappan, G. Gopikrishna
FISH & SHELLFISH IMMUNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Chris J. Hulatt, Irina Smolina, Adam Dowle, Martina Kopp, Ghana K. Vasanth, Galice G. Hoarau, Rene H. Wijffels, Viswanath Kiron
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biotechnology & Applied Microbiology
Serena Lima, Peter S. C. Schulze, Lisa M. Schuler, Ralf Rautenberger, Daniela Morales-Sanchez, Tamara F. Santos, Hugo Pereira, Joao C. S. Varela, Francesca Scargiali, Rene H. Wijffels, Viswanath Kiron
Summary: The periodic emission of light pulses by LEDs can stimulate growth or induce high value biocompounds in microalgae. Low-frequency flashing light can increase the productivity of proteins, polyunsaturated fatty acids, and pigments in algae biomass.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Fisheries
Prasannan Geetha Preena, Vattiringal Jayadradhan Rejish Kumar, Isaac Sarojini Bright Singh
Summary: One of the major challenges in sustainable aquaculture production systems is the accumulation of nitrogenous waste. By establishing in situ nitrification and denitrification through biofilters/bioreactors activated with nitrifying/denitrifying bioaugmentors, total nitrogen removal can be achieved without external carbon supplements or additional anaerobic compartment. The structure and population dynamics of nitrifying/denitrifying consortia in recirculating aquaculture systems are influenced by environmental factors, which are crucial for the success of the processes.
REVIEWS IN AQUACULTURE
(2021)
Article
Biotechnology & Applied Microbiology
B. S. Anoop, Jayesh Puthumana, Vrinda Sukumaran, Cijo George Vazhappilly, Salini Kombiyil, Rosamma Philip, Isaac Sarojini Bright Singh
Summary: The development of shrimp cell lines has been attempted for over 30 years, with one of the main challenges being replicative senescence and inability to maintain telomere length in vitro. By inducing oncogenic transformation through a recombinant baculovirus containing H-ras downstream of the shrimp viral promoter IHHNV-P2, a fibroblast-like primary cell culture with confirmed H-ras and GFP expression was successfully developed. Transduction efficiency during early dividing stage of cells can be enhanced by adding 5 mM sodium butyrate to the culture medium.
MARINE BIOTECHNOLOGY
(2021)
Article
Chemistry, Medicinal
Giacomo Zaccone, Gioele Capillo, Jorge Manuel Oliveira Fernandes, Viswanath Kiron, Eugenia Rita Lauriano, Alessio Alesci, Patrizia Lo Cascio, Maria Cristina Guerrera, Michal Kuciel, Krystyna Zuwala, Jose Manuel Icardo, Atsushi Ishimatsu, Ryosuke Murata, Takafumi Amagai, Antonino Germana, Marialuisa Aragona
Summary: Antimicrobial peptides (AMPs) are multifunctional peptides with antimicrobial and immunomodulatory activities. This review focuses on the molecular characterization of the fish-specific AMP piscidin1 and its role in the immune response against pathogens. The discovery of piscidins in the gill and skin and their potential as drug targets for antimicrobial barrier function and aquaculture therapy is important for future research.
Article
Biotechnology & Applied Microbiology
A. Panigrahi, P. Esakkiraj, C. Saranya, R. R. Das, M. Sundaram, N. S. Sudheer, I. F. Biju, M. Jayanthi
Summary: This study demonstrates the probiotic effect of bio-augmented Bacillus tequilensis AP BFT3 on the production, immune response, and proteomic changes of Penaeus vannamei reared in a biofloc system. Adding the probiotic in the biofloc rearing significantly improves shrimp growth, survival, and water quality. The expression of immune-related genes is upregulated, and differential protein expression is observed. Adding B. tequilensis AP BFT3 as a probiotic in a biofloc system reduces the incidence of diseases during culture.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2022)
Article
Fisheries
Soumya Balakrishnan, Ahna Ameer, Sowmya Pazhur Mohandas, Ambadi Kannan Maliyekkal Sajeevan, Anoop Bhaskaran Sathyabhama, Bright Singh
Summary: The study demonstrates that pyocyanin is an effective and environmentally friendly aquaculture drug for controlling Vibrio spp. in RAS-based shrimp culture without impeding nitrification by nitrifying bacteria.
AQUACULTURE INTERNATIONAL
(2022)
Article
Fisheries
Ancy Thomas, Naduvilamuriparampu Saidumuhammed Sudheer, Viswanath Kiron, Bright Singh Issac Singh, Rangarajan Badri Narayanan
Summary: This paper investigates the effectiveness of the major antigenic proteins VP24 and VP28 from white spot syndrome virus (WSSV) in inducing an immune response in shrimp. The recombinant proteins were mass-produced and orally administered to shrimp, resulting in the upregulation of several biodefense genes. The study reveals the antigenic properties of VP24 and VP28 and their ability to elicit an immune response after oral administration.
AQUACULTURE RESEARCH
(2022)
Review
Biotechnology & Applied Microbiology
Soumya Balakrishnan, I. S. Bright Singh, Jayesh Puthumana
Summary: This review discusses the obstacles and importance of developing marine molluscan cell lines, focusing on the advancements and future trends in the past decade.
Article
Food Science & Technology
Viswanath Kiron, Maria Hayes, Dorit Avni
Summary: Inflammatory bowel disease (IBD) was originally associated with industrialized nations but has now spread globally due to the popularity of ultra-processed food and lack of proper strategies to avoid foodborne microbes. Imbalance in bacteria, intestinal permeability, and dysbiosis are key factors in IBD. Using probiotics, prebiotics from algae, and a synbiotic strategy could help combat IBD by controlling pathogen proliferation and promoting beneficial bacteria growth.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2022)
Article
Virology
Dominic Vithayathil Diya Dominic, Thavarool Puthiyedathu Sajeevan, Ahna Ameer, Abdulkhader Muneer, Linu Eldho, Bhaskaran Sathyabhama Anoop, Puthumana Jayesh, Valsamma Joseph, Rosamma Philip, Isaac Sarojini Bright Singh
Summary: Infectious hypodermal hematopoietic necrosis virus (IHHNV/PstDVI) was successfully isolated and propagated in a hybrid shrimp-insect cell line PmLyO-Sf9. Cytopathic changes such as clustering, enlargement, syncytium formation, and cell death were observed in the infected cell line, indicating viral replication. The purified virus was found to be infectious both in vitro and in vivo, providing insights for studying the molecular mechanism of IHHNV infection and developing antiviral strategies in aquaculture.
ARCHIVES OF VIROLOGY
(2023)
Article
Fisheries
Giacomo Zaccone, Alessio Alesci, Doaa M. M. Mokhtar, Marialuisa Aragona, Maria Cristina Guerrera, Gioele Capillo, Marco Albano, Jorge de Oliveira Fernandes, Viswanath Kiron, Ramy K. A. Sayed, Marwa M. M. Hussein, Patrizia Lo Cascio, Michal Kuciel, Krystyna Zuwala, Antonino Germana, Jose Manuel Icardo, Eugenia Rita Lauriano
Summary: The recognition and elimination of invading pathogens are essential for host survival. Macrophages, which function similarly to vertebrate macrophages, play a crucial role in host protection in all multicellular organisms. CD14 is a pattern responsible for bacterial recognition found on the surface of macrophages. These cells store antimicrobial molecules and display polarization states similar to mammalian macrophages. The present study reports the presence of various cell markers, including CD14, in the intestinal macrophages of catfish and bonytongue, providing insights into the in vivo function of macrophages in fish.
Article
Fisheries
Solveig Lysfjord Sorensen, Ateshm Ghirmay, Yangyang Gong, Dalia Dahle, Ghana Vasanth, Mette Sorensen, Viswanath Kiron
Summary: The study found that the fish meal-based diet showed the best growth performance, followed by the pre-extruded Nannochloropsis diet. Feeding of unprocessed Nannochloropsis and Tetraselmis resulted in reduced enterocyte vacuolization. There was a significant effect of processing on fillet fatty acid content, intestine and liver structure, and the expression of selected genes in the liver.
