期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 112, 期 1, 页码 88-93出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2012.09.005
关键词
Bifidobacterium; Japanese honeybee; European foulbrood; Melissococcus plutonius
类别
资金
- program for the Promotion of Basic Research Activities for Innovative Biosciences (PRO-BRAIN)
- Japan Society for the Promotion of Science [22580345]
- Grants-in-Aid for Scientific Research [22580345] Funding Source: KAKEN
Bifidobacteria were isolated from the intestinal tract of the Japanese honeybee, Apis cerana japonica, and investigated for potential application as a probiotic agent against Melissococcus plutonius, the causal agent of European foulbrood (EFB), based on the findings of in vitro inhibition assays. A total of 11 bifidobacteria strains (designated as AcjBF1-AcjBF11) were isolated using a culture-dependent method and their 16S rRNA gene sequences were analyzed. The AcjBF isolates belonged to three distinct bifidobacterial phylotypes that were similar to those found in the European honeybee, Apis mellifera. Although the Japanese and European honeybees are distinct species with different traits and habits, the observation that they share highly similar bifidobacterial phylotypes suggests that bifidobacteria are conserved among honeybee species. Despite having extremely high 16S rRNA gene sequence similarities, the AcjBF isolates had markedly different carbohydrate fermentation profiles. In addition, in vitro growth inhibition assays revealed that the cell-free supernatants of all AcjBF isolates exhibited antagonistic effects on M. plutonius growth. These results indicate that the bifidobacteria isolated from the gut of Japanese honeybee could potentially be employed as a new biological agent to control EFB. (C) 2012 Elsevier Inc. All rights reserved.
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