期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 107, 期 1, 页码 65-68出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2011.02.002
关键词
Litopenaeus vannamei; Consecutive WSSV inoculations; Extended susceptibility reduction; Inhibition of virus replication; dsRNA
类别
资金
- CONACyT (Mexico) [205997]
- PISA Consortium [60030-CONACyT]
The antiviral effect of vp28 or vp26 double-stranded (ds) RNA upon single or consecutive white spot syndrome virus (WSSV) intramuscular challenges with a high infectious dose was evaluated. The vp28 dsRNA showed the highest protection both in single (LT50 = 145 h at 10 d and 98 h at 20 d post treatment [dpt]) or consecutive (LT50 = 765 h) WSSV challenges compared to vp26 dsRNA (LT50 = 126 h at 10 d and 57 h at 20 dpt vs. consecutive challenge LT50 = 751 h). Single WSSV challenges showed that animals treated with vp28 or vp26 dsRNA gradually lost the antiviral effect as virus challenge occurred at 10 dpt (cumulative mortality 63% vs. 80%, respectively) or 20 dpt (87% vs. 100%, respectively). In contrast, animals treated with vp28 or vp26 dsRNA and consecutively challenged with WSSV showed and extended lower susceptibility to WSSV. All dead animals were WSSV-positive by one-step PCR, whereas all surviving shrimp from single or continuous challenges were WSSV-negative as determined by reverse transcription (RT)-PCR. In conclusion, shrimp treated with a single administration of vp28 or vp26 dsRNA and consecutively challenged with WSSV showed a stronger and longer antiviral response than shrimp exposed once to WSSV at 10 or 20 dpt. (C) 2011 Elsevier Inc. All rights reserved.
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