期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 106, 期 3, 页码 400-406出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2010.11.008
关键词
BbCre1; Carbon catabolite repressor; Virulence; Beauveria bassiana
类别
资金
- Ministry of Science and Technology of China [2009CB118904]
- Hi-Tech Research and Development Program of China [2006AA10A212]
A gene (BbCre1, GenBank accession number EF108309) encoding a carbon catabolite repressor (CreA) with two Cys(2)His(2) zinc finger regions and a nuclear localization signal was cloned from the entomopathogenic fungus Beauveria bassiana. Overexpression and antisense strategies were used to investigate the biological functions of this gene. Compared with the wild type, the conidial yield and colony growth of BbCre1-overexpression transformants were significantly decreased on the plates with xylose or ethanol as the sole carbon source. With glucose as the sole carbon source, a significant difference was observed in the activity of Pr1A-like protease among BbCre1-overexpression transformants, antisense-BbCre1 transformants and the wild type. However, bioassays showed that knockdown or overexpression of BbCre1 did not have a significant impact on the virulence of B. bassiana to aphids. These results imply that the fungus remains virulent, even when simpler, less expensive nutrients are available, i.e. glucose. (C) 2010 Elsevier Inc. All rights reserved.
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