期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 99, 期 2, 页码 220-226出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2008.05.004
关键词
coffee; coffee berry borer; biocontrol; Metaihizium anisopliae; AaIT; scorpion neurotoxin; Androctonus austialis insect toxin
类别
资金
- University of Maryland
- National Federation of Coffee Growers of Colombia
Coffee berry borer (CBB) is the Worlds most devastating coffee pest causing an estimated US$500 million worth of losses annually through damage and control costs. Beauveria bassiona and Metarhizium anisopliae have been employed to control this pest but their low virulence (slow kill and large inoculums) is an important factor constraining their use. M. anisopliae (AaIT-Ma549) has been modified to express the scorpion toxin (AaIT) in insect hemolymph and this greatly increased pathogenicity against Manduca sexta and Aedes aegypti. Here, we demonstrate that AaIT-Ma549 was also dramatically more virulent against CBB, and we provide a much more comprehensive analysis of infection processes and post-mortality development than in the previous research. We evaluated several spore concentrations (10(1) through 10(7) spores/ml) of both the wild type and recombinant strain. At concentrations of 10(1), 10(1) and 10(3) spores/ml, the recombinant strain significantly increased mortality of CBB by 32.2%. 56.6% and 24.6%, respectively. The medial lethal concentration (LC50) was reduced 15.7-fold and the average survival time (AST) was reduced by 20.1 % to 2.98 +/- 0.1 days with 10(7) spores/ml. This is the first occasion that an entomopathogenic fungus has been found to kill CBB in less than 3 days. However, AaIT-Ma549 produces significantly fewer spores on cadavers than the parental strain. (c) 2008 Elsevier Inc. All rights reserved.
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