Article
Chemistry, Multidisciplinary
Qiang Liu, Gunnar Knobloch, Jim Voorneveld, Nico J. Meeuwenoord, Herman S. Overkleeft, Gijsbert A. van Der Marel, Andreas G. Ladurner, Dmitri Filippov
Summary: The translation discusses the significance of ADP-ribosylation and the chemical synthesis of linear ADP-ribose oligomers, as well as their affinity for ALC1. The study reveals the length-dependent binding manner of ADPr oligomers to ALC1, illustrating how the activity of this molecular machine is controlled by PAR.
Review
Oncology
Keun-Yeong Jeong, Minhee Park
Summary: PARP-1 and PARylation are crucial factors in the malignant formation of CRC, playing roles in DNA repair, regulation of epigenetic markers and chromatin structure, and are at the forefront of pathological changes required for CRC progression. This understanding could help in targeting PARP-1 and PARylation for potential treatments and various research challenges in both nonclinical and clinical settings.
WORLD JOURNAL OF GASTROINTESTINAL ONCOLOGY
(2021)
Article
Gastroenterology & Hepatology
Keun-Yeong Jeong, Jae Jun Sim, Minhee Park, Hwan Mook Kim
Summary: This study found that sustained calcium supply induced excessive PAR accumulation in pancreatic cancer cells, leading to increased mitochondrial ROS levels, decreased PAR glycohydrolase and ADP-ribosyl hydrolase 3 levels, and energy deprivation. Further research confirmed that this process was accompanied by elevated AIF levels and had antitumor effects.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Genetics & Heredity
Chin Hong Lee, Nathaniel P. Hawker, Jonathan R. Peters, Thierry G. A. Lonhienne, Nial R. Gursanscky, Louisa Matthew, Christopher A. Brosnan, Christopher W. G. Mann, Laurence Cromer, Christelle Taochy, Quy A. Ngo, Venkatesan Sundaresan, Peer M. Schenk, Bostjan Kobe, Filipe Borges, Raphael Mercier, John L. Bowman, Bernard J. Carroll
Summary: The DEM1 and DEM2 genes play essential roles in cell division and gamete viability in plants, with DEM2 playing a more important role than DEM1. DEM proteins interact with the highly conserved RAN protein, which controls cell division in all eukaryotic organisms.
Article
Cell Biology
Arnold J. Muccini, Margaret A. Gustafson, J. Christopher Fromme
Summary: This study presents cryoEM structures of Gea2, a yeast paralog of human Arf-GEF GBF1, revealing its regulatory domain organization and its binding mechanism to the Golgi membrane. The conformational switching of Gea2 is proposed to promote membrane insertion of Arf1 during the nucleotide exchange reaction.
Article
Multidisciplinary Sciences
Balint Dudas, David Perahia, Erika Balog
Summary: RalF, an Arf GEF from Legionella pneumophilia, is autoinhibited in its crystal structure. The mechanism of lifting this autoinhibition is unknown. Molecular Dynamics alone is not efficient to determine the active structure of RalF, but using MDeNM combined with experimental findings bridged the gap between experiments and simulation.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Zhuofu Ni, Xiaodong Cheng
Summary: EPAC proteins are only present in multicellular Metazoa, with EPAC1 only associated with chordates and EPAC2 spanning the entire animal kingdom. EPAC1 has undergone more selection and evolved faster than EPAC2.
Article
Cell Biology
Bing-Ru Yan, Taoyingnan Li, Etienne Coyaud, Estelle M. N. Laurent, Jonathan St-Germain, Yuhuan Zhou, Peter K. Kim, Brian Raught, John H. Brumell
Summary: The protein C5orf51 plays a crucial role in regulating the activity of RAB7A during mitophagy, facilitating the shuttle of RAB7A between late endosomes and mitochondria. Depletion of C5orf51 leads to compromised localization of RAB7A on depolarized mitochondria, degradation by the proteasome, and inhibition of ATG9A recruitment to depolarized mitochondria.
Review
Oncology
Simon Manuel Tria, Matthew E. Burge, Vicki L. J. Whitehall
Summary: More than a third of colorectal cancers have a KRAS mutation, posing a challenge for the development of direct targeting inhibitors. Recent advancements have led to the development of new generation inhibitors specifically targeting the G12C KRAS mutation, such as Adagrasib and Sotorasib. Therapeutic regimens are being developed to address resistance to these inhibitors and improve treatment outcomes.
Article
Multidisciplinary Sciences
Mara C. Harwood, Tai-Ting Woo, Yuka Takeo, Daniel DiMaio, Billy Tsai
Summary: During HPV entry, the virus is trafficked through the cell compartments, including the endosome, trans-Golgi network (TGN), and Golgi apparatus. The coat protein complex I (COPI) is identified as a host factor required for HPV infection. The interaction between the cytoplasmic segment of HPV L2 and COPI facilitates the trafficking of HPV through the TGN and Golgi stacks, ultimately reaching the nucleus.
Article
Oncology
Xin Zhang, Liangliang Ren, Junhua Wu, Rongni Feng, Yunyang Chen, Ronggang Li, Meimei Wu, Mingzhu Zheng, Xing Gui Wu, Wanjun Luo, Hongle He, Yanming Huang, Miaoling Tang, Jun Li
Summary: ARHGEF37 is upregulated in HCC and associated with tumor invasiveness, pulmonary metastasis and poor prognosis. Overexpression of ARHGEF37 enhances HCC cell extravasation and metastasis by promoting cell adhesion and migration. ARHGEF37 activates Cdc42 to promote invadopodia formation in HCC cells, disrupting the interaction between endothelial cells and pericytes.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Aaron M. N. Joiner, Ben P. Phillips, Kumar Yugandhar, Ethan J. Sanford, Marcus B. Smolka, Haiyuan Yu, Elizabeth A. Miller, J. Christopher Fromme
Summary: The study revealed the regulation of Rab1 activation by the TRAPPIII complex, with the Trs85 subunit serving as a membrane anchor for the entire complex via its amphipathic helix. These findings provide a structural understanding of Rab activation on organelle and vesicle membranes.
Article
Biochemistry & Molecular Biology
Xiaobing Kou, Qianke He, Peng Cao, Peng Wang, Shaoling Zhang, Juyou Wu
Summary: This study compared the function of Rho GTPase regulators in seven Rosaceae species. Genome duplication or dispersed duplication events facilitated the expansion of GEF, GAP, and GDI families. Protein-protein interactions indicated that PbrGDI1 regulated the growth of the pear pollen tube through PbrROP1 signaling downstream.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Maria K. Mateyak, Dongming He, Pragati Sharma, Terri Goss Kinzy
Summary: Phosphorylation of eEF1A affects its function, and mutation analysis confirms the importance of several phosphorylation sites, with the S289D mutant's activity being influenced by eEF1B alpha.
Article
Multidisciplinary Sciences
Giulia Fasano, Valentina Muto, Francesca Clementina Radio, Martina Venditti, Niloufar Mosaddeghzadeh, Simona Coppola, Graziamaria Paradisi, Erika Zara, Farhad Bazgir, Alban Ziegler, Giovanni Chillemi, Lucia Bertuccini, Antonella Tinari, Annalisa Vetro, Francesca Pantaleoni, Simone Pizzi, Libenzio Adrian Conti, Stefania Petrini, Alessandro Bruselles, Ingrid Guarnetti Prandi, Cecilia Mancini, Balasubramanian Chandramouli, Magalie Barth, Celine Bris, Donatella Milani, Angelo Selicorni, Marina Macchiaiolo, Michaela V. Gonfiantini, Andrea Bartuli, Riccardo Mariani, Cynthia J. Curry, Renzo Guerrini, Anne Slavotinek, Maria Iascone, Bruno Dallapiccola, Mohammad Reza Ahmadian, Antonella Lauri, Marco Tartaglia
Summary: Disruption to the ER-Golgi network can cause neurodevelopmental disorders, and ARF3 mutations have been identified as the cause of a rare pediatric neurological disorder. This study provides detailed molecular characterization of ARF3 mutations and demonstrates their impact on Golgi morphology, vesicles assembly, and trafficking.
NATURE COMMUNICATIONS
(2022)
