Article
Plant Sciences
Maribela Pestana, Pedro Garcia-Caparros, Teresa Saavedra, Florinda Gama, Javier Abadia, Amarilis de Varennes, Pedro Jose Correia
Summary: This study evaluated the tolerance to iron deficiency of five citrus rootstocks and found that micronutrient homeostasis in roots and shoots of all rootstocks were affected by iron stress conditions. Additionally, the Fe/Cu ratio was significantly related to total leaf chlorophyll, which may be used to assess rootstock performance.
Article
Agronomy
Florinda Gama, Teresa Saavedra, Susana Dandlen, Pedro Garcia-Caparros, Amarilis de Varennes, Gustavo Nolasco, Pedro Jose Correia, Maribela Pestana
Summary: This study used the VIGS method to investigate the role of the FRO1 gene in tomato plants and its impact on the regulation of root FCR activity and nutritional homeostasis.
JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE
(2023)
Article
Horticulture
Fabian Perez-Labrada, Adalberto Benavides-Mendoza, Antonio Juarez-Maldonado, Susana Solis-Gaona, Susana Gonzalez-Morales
Summary: Iron deficiency is a common abiotic stress for plants in calcareous soils. The application of citric acid (CA) and humic-like substances (HLS) can improve iron nutrition by inducing temporal alterations in enzyme activity and gene expression.
Article
Agronomy
Takanori Kobayashi, Keisuke Maeda, Yutaro Suzuki, Naoko K. Nishizawa
Summary: Iron is an essential micronutrient with low solubility under alkaline conditions. Iron deficiency in calcareous soils affects plant productivity, while iron deficiency anemia is a common problem in humans, especially in Asian and African populations. Transgenic manipulation of genes involved in iron uptake and storage has shown improvements in iron deficiency tolerance and accumulation in plants. This study introduces the simultaneous expression of OsHRZ knockdown and OsIRT1 promoter-Refre1/372 genes, resulting in enhanced iron deficiency tolerance and accumulation in rice plants.
Article
Plant Sciences
Teresa Saavedra, Florinda Gama, Maria A. Rodrigues, Javier Abadia, Amarilis de Varennes, Maribela Pestana, Jose Paulo Da Silva, Pedro Jose Correia
Summary: The economic costs and environmental impacts of iron-chelate treatments have led researchers to explore alternative methods for controlling iron deficiency chlorosis. This study examined the effects of applying malic acid, citric acid, and succinic acid to strawberry plants and found that succinic acid improved chlorophyll levels and altered the overall nutritional balance in the plants.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jie Xiao, Yoo Gyeong Park, Ge Guo, Byoung Ryong Jeong
Summary: The growth and development of S. commixta in vitro was affected by both the Fe source and pH, with plantlets showing greener leaves under pH 4.70 and 5.70. Despite higher Fe absorption at pH 4.70, Fe-EDTA combined with pH 5.70 was found to be the most beneficial for the overall growth and development of S. commixta.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agronomy
Joao Vieira, Luna Dzidic-Uzelac, Izabela Thais dos Santos, Marco Patricio, Florinda Gama, Teresa Saavedra, Pedro Jose Correia, Maribela Pestana
Summary: The response of Chia plants to Fe deficiency was assessed in this preliminary study. The study found that under iron-deficient conditions, Chia plants exhibited symptoms of iron chlorosis, reduced biomass, morphological changes in roots, and significantly lower ferric chelate-reductase activity in roots.
NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Clarissa Challam, Som Dutt, Duraialagaraja Sudhakar, Muthurajan Raveendran, Tanuja Buckseth, Rajesh Kumar Singh
Summary: The experiment demonstrated a significant increase in lateral root growth of potato genotype under iron-stress, with total lateral root number being closely linked to ferric-chelate reductase activity. These findings suggest that FCR plays a role in root Fe uptake in potato and may contribute to Fe distribution throughout the plant, indicating a potential trait for identifying tolerance to chlorosis and nutrient-deficiency stress.
INDIAN JOURNAL OF AGRICULTURAL SCIENCES
(2021)
Article
Plant Sciences
Qingguo Du, Wenshuai Lv, Yu Guo, Juan Yang, Shanhong Wang, Wen-Xue Li
Summary: This study reveals the importance of miR164 in plant responses to iron deficiency and its role in regulating the NAC5-NFYA8 module. The NAC domain transcription factor5 protein plays a crucial role in Arabidopsis tolerance to iron deficiency by promoting the expression of Nuclear Factor Y, Subunit A8.
Article
Plant Sciences
Macarena Angulo, Maria Jose Garcia, Esteban Alcantara, Rafael Perez-Vicente, Francisco Javier Romera
Summary: Iron is an essential micronutrient for plants, playing vital roles in plant growth and metabolism. Plants acquire iron by developing morphological and physiological responses in their roots, with molecules like ethylene and nitric oxide playing crucial roles in this process.
Article
Biochemistry & Molecular Biology
Laura Ceballos-Laita, Daisuke Takahashi, Matsuo Uemura, Javier Abadia, Ana Flor Lopez-Millan, Jorge Rodriguez-Celma
Summary: Iron and manganese are essential elements for plants, but they compete for the same uptake transporters and show conflicting interactions at the regulatory level. Proteomic techniques were used to study the proteome profiles of tomato roots grown under iron or manganese deficiency. The results showed that both deficiencies led to common cell wall remodelling, but differed greatly in terms of oxidative stress, coumarin production, protein, nitrogen, and energy metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Tirthankar Bandyopadhyay, Manoj Prasad
Summary: Iron is essential for plant growth and different plants employ different iron uptake strategies to maintain iron homeostasis through a series of physiological processes. Recent research has led to a better understanding of the ecophysiology of iron transport.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Plant Sciences
Kasper van Gelderen, Chiakai Kang, Peijin Li, Ronald Pierik
Summary: Plants respond to environmental changes through low Red:Far-Red and low nitrate signaling, which may interact with each other. It is unclear which response is prioritized when both signals co-occur.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Uneeb Urwat, Syed Mudasir Ahmad, Antonio Masi, Nazir Ahmad Ganai, Imtiyaz Murtaza, Imran Khan, Sajad Majeed Zargar
Summary: This study investigated the impact of Fe and Zn stress on the growth and development of common bean, revealing differential gene expression patterns under Fe and Zn stress conditions. The identified genes highly responsive to Fe and Zn stress could potentially be used to overcome mineral stress in dicot crops.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Multidisciplinary
Marta Fuentes, German Bosch, David de Hita, Maite Olaetxea, Javier Erro, Angel Ma Zamarreno, Jose Ma Garcia-Mina
Summary: Previous studies have shown that natural heteromolecular complexes can be an alternative to synthetic chelates for correcting iron deficiency. This study investigated the interaction of these complexes with Ca2+ at alkaline pH, their stability, root uptake in cucumber, and chemical structure using molecular modeling.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Xing Xing Liu, Hai Hua Zhang, Qing Yang Zhu, Jia Yuan Ye, Ya Xin Zhu, Xiang Ting Jing, Wen Xin Du, Miao Zhou, Xian Yong Lin, Shao Jian Zheng, Chong Wei Jin
Summary: This study reveals that ammonium affects root development in Arabidopsis through different mechanisms than nitrate. The cell wall-localized ferroxidase LPR2 plays a critical role in attenuating root growth in response to ammonium.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Yu Xin Wen, Jia Yi Wang, Hui Hui Zhu, Guang Hao Han, Ru Nan Huang, Li Huang, Yi Guo Hong, Shao Jian Zheng, Jian Li Yang, Wei Wei Chen
Summary: In this study, the researchers report the key role of the methylation enzyme SlDRM7 in tomato plant growth and development, particularly in leaf interveinal chlorosis and senescence. Silencing of SlDRM7 led to developmental defects and alterations in methylation and gene expression levels of genes involved in chlorophyll synthesis, photosynthesis, and starch degradation. SlDRM7 was also found to be activated during aging- and dark-induced senescence.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Yun Qi Wei, Jun Jie Yuan, Chen Chen Xiao, Gui Xin Li, Jing Ying Yan, Shao Jian Zheng, Zhong Jie Ding
Summary: The ULS1 and ULS2 genes play a crucial role in leaf senescence by regulating the transport of ABA. They function as feedback inhibitors of ABCG40-dependent ABA transport during ABA-induced leaf senescence and stomatal closure.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Huihui Zhu, Jiayi Wang, Dan Jiang, Yiguo Hong, Jiming Xu, Shaojian Zheng, Jianli Yang, Weiwei Chen
Summary: This study reveals that the SPL transcription factor SlSPL-CNR negatively regulates Fe-deficiency responses in tomato roots, with its expression being controlled by the microRNA SlymiR157. SlSPL-CNR regulates the Fe homeostasis by interacting with specific motifs in the SlbHLH101 promoter.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Plant Sciences
Xiang P. Liu, Li J. Gao, Ben T. She, Gui X. Li, Yun R. Wu, Ji M. Xu, Zhong J. Ding, Jian F. Ma, Shao J. Zheng
Summary: Through mutant screening and complementation tests, we identified the Al-sensitivity associated gene ArPK in rice and revealed its role in regulating rice Al resistance. ArPK acts as an endogenous inhibitor of ODC1 activity under Al treatment, maintaining appropriate levels of endogenous Put, and thus plays a protective role in root growth.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Plant Sciences
Fiona Jamieson, Zhe Ji, Eric J. Belfield, Zhong Jie Ding, Shao Jian Zheng, Nicholas P. Harberd
Summary: Genetic analysis reveals the important role of ethylene signaling in regulating nitrogen metabolism in Arabidopsis thaliana. Ethylene signaling can regulate nitrate assimilation enzyme activity to control nitrogen metabolism.
Article
Biochemistry & Molecular Biology
Qi Yu He, Jian Feng Jin, He Qiang Lou, Feng Feng Dang, Ji Ming Xu, Shao Jian Zheng, Jian Li Yang
Summary: Phosphocholine metabolism plays an important role in root development and salt stress tolerance in Arabidopsis thaliana by regulating glycerolipid metabolism, reactive oxygen species accumulation and distribution, and auxin distribution.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Nannan Su, Aiqin Zhu, Xin Tao, Zhong Jie Ding, Shenghai Chang, Fan Ye, Yan Zhang, Cheng Zhao, Qian Chen, Jiangqin Wang, Chen Yu Zhou, Yirong Guo, Shasha Jiao, Sufen Zhang, Han Wen, Lixin Ma, Sheng Ye, Shao Jian Zheng, Fan Yang, Shan Wu, Jiangtao Guo
Summary: The PIN3 protein plays a crucial role in plant growth and development. This study used cryo-electron microscopy to reveal the dimeric structure of PIN3 and its mechanism in auxin transport. The researchers found that PIN3 forms a large extracellular cavity at the dimer interface and adopts an inward-facing conformation. The structural and functional analyses provided insights into the recognition of substrates and inhibitors by PIN3, as well as the molecular mechanism of inhibition on auxin transport.
Article
Plant Sciences
Jia Yuan Ye, Miao Zhou, Qing Yang Zhu, Ya Xin Zhu, Wen Xin Du, Xing Xing Liu, Chong Wei Jin
Summary: Iron deficiency is a significant constraint for plant growth in calcareous soils. This study reveals that the nitrate transporter NRT1.1 in Arabidopsis plants is downregulated under iron deficiency, but its loss of function alleviates iron deficiency chlorosis. Additionally, disruption of NRT1.1 prevents apoplastic alkalization and facilitates the reutilization of iron in shoots under iron-deficient conditions.
Article
Biochemistry & Molecular Biology
Li Jun Gao, Xiang Pei Liu, Ke Ke Gao, Meng Qi Cui, Hui Hui Zhu, Gui Xin Li, Jing Ying Yan, Yun Rong Wu, Zhong Jie Ding, Xue Wei Chen, Jian Feng Ma, Nicholas P. Harberd, Shao Jian Zheng
Summary: The modification of cell wall properties plays an important role in the resistance of rice to aluminum (Al) toxicity. Exogenous application of putrescines (Put) can restore the Al resistance of art1 mutant in rice, and this is achieved by reducing the Al accumulation in the cell wall of root tips. The R2R3 MYB transcription factor OsMYB30 has been identified as a target in both ART1-dependent and Put-promoted Al resistance.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Ying Huang, Jing An, Sanchari Sircar, Clara Bergis, Chloe Dias Lopes, Xiaoning He, Barbara Da Costa, Feng-Quan Tan, Jeremie Bazin, Javier Antunez-Sanchez, Maria Florencia Mammarella, Ravi-sureshbhai Devani, Rim Brik-Chaouche, Abdelhafid Bendahmane, Florian Frugier, Chongjing Xia, Christophe Rothan, Aline V. Probst, Zouine Mohamed, Catherine Bergounioux, Marianne Delarue, Yijing Zhang, Shaojian Zheng, Martin Crespi, Sotirios Fragkostefanakis, Magdy M. Mahfouz, Federico Ariel, Jose Gutierrez-Marcos, Cecile Raynaud, David Latrasse, Moussa Benhamed
Summary: This study examines the changes in chromatin and gene expression in response to heat stress in tomato. They found that heat stress rapidly alters chromatin structure and leads to the formation of temporary contacts between promoters and enhancers, which drive the expression of genes responsive to heat stress. The researchers also discovered that this spatial reorganization of chromatin requires the transcription factor HSFA1a.
NATURE COMMUNICATIONS
(2023)
Review
Plant Sciences
Xing Xing Liu, Xiao Fang Zhu, Da Wei Xue, Shao Jian Zheng, Chong Wei Jin
Summary: Iron (Fe) is a crucial micronutrient for plants and is stored in the apoplast as an important Fe pool. Plants have developed strategies to re-utilize this apoplastic Fe pool to adapt to Fe deficiency and other stresses. This review focuses on the relevance of apoplastic Fe in modulating plant behavior changes in response to stress cues, specifically discussing the components involved in stress signaling networks.
TRENDS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Huihui Zhu, Guanghao Han, Jiayi Wang, Jiming Xu, Yiguo Hong, Li Huang, Shaojian Zheng, Jianli Yang, Weiwei Chen
Summary: Iron (Fe) is an essential micronutrient for all organisms. However, its limited bioavailability restricts plant growth, yield, and nutritional quality. The transcriptional regulation of plant responses to Fe deficiency has been extensively studied, but the contribution of DNA methylation remains poorly understood. In this study, it was found that DNA methylation plays a role in regulating Fe deficiency responses in tomato roots. Dynamic changes in the DNA methylome in response to Fe deficiency were identified, with differentially methylated regions (DMRs) occurring at different sites depending on the duration of Fe deficiency. Furthermore, the changes in DNA methylation levels did not correlate with the changes in gene expression levels, except for the CG hypermethylation at the bHLH39 promoter, which was positively correlated with its transcriptional induction. The involvement of bHLH39 in the modulation of Fe homeostasis was confirmed through gene silencing and luciferase reporter assays. Overall, these findings suggest that dynamic epigenetic DNA methylation at the CG context at the bHLH39 promoter contributes to the Fe deficiency response in tomato.
HORTICULTURE RESEARCH
(2023)
Article
Plant Sciences
Yan Wang, Fei Wang, Hong Lu, Rongbin Lin, Jiaming Liu, Yu Liu, Jiming Xu, Yunrong Wu, Zhiye Wang, Ming Zhou, Xiaorong Mo, Zhongchang Wu, Huixia Shou, Shaojian Zheng, Chuanzao Mao
Summary: In this study, the biological function and mode of action of OsHMGB1 were investigated. OsHMGB1 positively regulates Pi homeostasis and plant growth by regulating the expression of PSR genes and increasing chromatin accessibility. This reveals a key epigenetic mechanism that fine-tunes plant acclimation responses to Pi-limited environments.