Article
Multidisciplinary Sciences
Tania Martin-Perez, Martina Koehsler, Julia Walochnik
Summary: This study evaluated the expression and stability of six commonly used housekeeping genes in N. gruberi and found significant variations in the stability of reference genes depending on the algorithms and experimental conditions. The geNorm, NormFinder, and RefFinder analysis identified ACT and G6PD as the most stable reference genes, while BestKeeper analysis identified 18S and TBP as the most stable reference genes. Additionally, normalizing the expression of the HSP90 gene with the most stable reference genes resulted in reliable gene expression, while using unstable reference genes led to unreliable results. This study has important implications for gene expression studies in N. gruberi.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Adriana Orrego, Maria Cecilia Gavilan, Anibal Arevalos, Belen Ortiz, Belen Gaete Humada, Amiliana Pineda-Fretez, Maria Cristina Romero-Rodriguez, Maria Eugenia Flores Giubi, Man Mohan Kohli, Julio C. M. Iehisa
Summary: This study evaluated the suitability of 12 candidate reference genes for gene expression analysis of M. phaseolina and identified CYP1 as the best reference gene. The findings provide valuable references for gene expression analysis during host infection with M. phaseolina.
Article
Biology
Liliana Sokol, Anne Cuypers, Anh-Co K. Truong, Ann Bouche, Katleen Brepoels, Joris Souffreau, Katerina Rohlenova, Stefan Vinckier, Luc Schoonjans, Guy Eelen, Mieke Dewerchin, Laura P. M. H. de Rooij, Peter Carmeliet
Summary: Translation of academic results into clinical practice is a significant challenge. In this study, the authors addressed this challenge by prioritizing and validating potential target genes from single-cell RNA-sequencing data. They focused on tip endothelial cell markers and identified four candidates that showed tip EC characteristics. This study highlights the importance of targeted assessment and prioritization of candidate genes for successful translation into clinical practice.
COMMUNICATIONS BIOLOGY
(2023)
Article
Genetics & Heredity
Kai Gao, Wasif Ullah Khan, Juan Li, Sai Huang, Xiong Yang, Ting Guo, Bin Guo, Ruqian Wu, Xinmin An
Summary: RT-qPCR is commonly used to analyze gene expression levels in different samples, but there is a lack of reliable reference genes for ornamental Koelreuteria species. This study identified phospholipase (PLA2) and beta-actin (ACT) as the most suitable and statistically reliable reference genes for gene expression studies in K. paniculata.
Article
Multidisciplinary Sciences
Guo-Hua Wang, Cheng-Cheng Liang, Bing-Zhi Li, Xin-Ze Du, Wen-Zhen Zhang, Gong Cheng, Lin-Sen Zan
Summary: This study assessed the accuracy of sixteen commonly used internal reference genes in skeletal muscle-derived satellite cells of Qinchuan cattle at different stages of proliferation and induction of differentiation, and determined the most suitable gene combinations. The results provide a foundation for selecting reference genes for qRT-PCR during the proliferation and induction of differentiation of bovine skeletal muscle-derived satellite cells.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Jing Tong, Manman Hu, Beibei Han, Yanhai Ji, Baoju Wang, Hao Liang, Mingchi Liu, Zhanhui Wu, Ning Liu
Summary: Chinese chive has unique flavor, nutrient, and medicinal values, and investigating its molecular mechanism on flavor formation and metabolic pathways is crucial. This study identified GLY1 and SKP1 as the most stable reference genes for normalization, highlighting the importance of validating reference gene stability before use. Using at least two reference genes for normalization can provide more accurate results, with the combination of GLY1 and SKP1 showing reliable normalization results in qRT-PCR experiments.
SCIENTIFIC REPORTS
(2021)
Article
Entomology
Xinhua Fu, Victor Benno Meyer-Rochow
Summary: This study evaluated the expression stability of eight widely used reference genes in Aquatica leii and recommended S27Ae and Reep5 for different life stages, Ef1a and Odc1 for different tissues, Tub and Odc1 for RNAi studies, and S27Ae and Tub for all treatments as the most appropriate reference genes for future molecular research. The results of this study will enhance the accuracy and reliability of RT-qPCR data normalization in A. leii for further molecular analysis.
Article
Multidisciplinary Sciences
Dayoung Oh, Ward De Spiegelaere, Hans J. Nauwynck
Summary: Porcine alveolar macrophages (PAMs) were used to study porcine respiratory viruses in vitro. Stable reference genes, PSAP and GAPDH, were identified for reliable normalization of target gene expression. This study highlights the importance of selecting appropriate reference genes for normalization and validation of RT-qPCR data in various experimental conditions. The identified reference genes will be useful for future studies on porcine respiratory viruses in PAMs.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Pramoda Kumar Sahoo, Sonali Parida, Subhadarshini Parida, Priyashree Parida, Anirban Paul
Summary: Argulosis is a economically significant freshwater fish ectoparasitic disease. Proper selection or normalization of reference genes is crucial for the accuracy of gene expression studies using real-time PCR. This study evaluated seven candidate reference genes and conducted comprehensive gene expression analysis in rohu carp infected with Argulus siamensis. The results identified stable and unstable reference genes and showed the role of immune modulation process in governing infection.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
T. Toorani, P. M. Mackie, G. F. Mastromonaco
Summary: Proper normalization of RT-qPCR data is crucial for interpreting results and ensuring the accuracy of scientific conclusions. In untreated bovine fibroblasts, a combined use of GUSB and RPL13A was determined to be the best approach for data normalization.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Chun-Di Xie, Bingyuan Wang, Zhao-Ji Shen, Wen-Ye Yao, Hong Ao, Bugao Li, Yangli Pei, Rong Zhou
Summary: This study investigated the expression of 15 reference genes in three pig cell lines and identified the most stable reference gene combinations in each cell line. The findings suggest that only two reference genes are needed for each cell line to standardize the expression of target genes.
BIOMED RESEARCH INTERNATIONAL
(2021)
Article
Multidisciplinary Sciences
Mary Wallace, Lynne K. Rieske
Summary: The study found that in the six-spined ips, Ips calligraphus, the 16s and 28s genes are the most stable and suitable reference genes for gene expression studies, especially in evaluating RNAi.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Conglong Lian, Bao Zhang, Jingfan Yang, JinXu Lan, Hao Yang, Kaihua Guo, Jingjing Li, Suiqing Chen
Summary: This study evaluated the expression stability of 12 candidate reference genes in different tissues and under different abiotic stresses in Isodon rubescens. The results identified the most stable reference genes for different tissues and treatments, and highlighted the importance of using appropriate reference genes in gene expression studies. This study provides valuable information for gene functional research in I. rubescens.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, Research & Experimental
Huikai Liu, Liwen He, Qianyu Cheng, Wenping Luo, Tianyu Zhao, Deqin Yang
Summary: The study identified superior reference genes for salivary gland development and functional regeneration. ACTG1 showed the highest stability during the developmental stage, while TUBB5 was recommended as the most stable reference gene for the regenerative stage in the SMG.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2022)
Article
Genetics & Heredity
Zhongxu Zhu, Keqin Gregg, Wenli Zhou
Summary: iRGvalid is a computational reference gene validation method based on high throughput gene expression data and double-normalization strategy. It can identify highly stable reference genes across different cancer types, offering an easy and robust tool for gene expression studies.
FRONTIERS IN GENETICS
(2021)
