Article
Entomology
Huan Liu, Xiaoyan Wang, Zihan Chen, Yongyue Lu
Summary: This study investigates the cold and heat tolerance of Bactrocera tau, an invasive pest that affects vegetables and fruits. The results show that B. tau has a wide temperature threshold for survival, which contributes to its successful establishment and expansion. The study also quantifies the tolerance potential of B. tau at different life stages.
Article
Biochemistry & Molecular Biology
Alex S. Torson, Meng Lei Zhang, Kevin Ong, Lamees Mohammad, Adam J. Smith, Daniel Doucet, Amanda D. Roe, Brent J. Sinclair
Summary: This study systematically characterized cold tolerance strategy and lower lethal limits of different life stages of the Asian longhorned beetle, revealing that larvae and eggs are the most cold-tolerant. Freeze-tolerant larvae showed a significant increase in hemolymph osmolality during cold exposure, while pupae exhibited sublethal effects before freezing.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Martin I. Daily, Thomas F. Whale, Peter Kilbride, Stephen Lamb, G. John Morris, Helen M. Picton, Benjamin J. Murray
Summary: By using a mineral ice nucleator, the issue of supercooling in microlitre liquid volumes during cryopreservation can be eliminated, leading to improved cell viability. This research opens up new possibilities for large-scale cryopreservation of mammalian cells, which can be applied in fields such as high throughput toxicology testing and regenerative medicine.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2023)
Article
Agronomy
Sayma Shammi, Ferdous Sohel, Dean Diepeveen, Sebastian Zander, Michael G. K. Jones
Summary: Current frost damage assessment techniques reliant on traditional data and manual inspection can be erroneous, labour-intensive, and lead to delayed decisions. This study explores a new technique using machine learning models trained on infrared thermal images to automatically detect freezing stages in on-field plants. Results show that the random forest model can detect frost events with the highest classification speed.
EUROPEAN JOURNAL OF AGRONOMY
(2023)
Article
Forestry
Zehai Hou, Yaxin Dong, Fengming Shi, Yabei Xu, Sixun Ge, Jing Tao, Lili Ren, Shixiang Zong
Summary: This study investigated the seasonal changes in the gut microbiome of D. valens during overwintering, revealing that changes in microbiome composition may be related to cold tolerance and antioxidant enzyme activity in the host.
Article
Plant Sciences
Masaya Ishikawa, Hiroyuki Ide, Tetsuya Tsujii, Timothy Stait-Gardner, Hikaru Kubo, Norihisa Matsushita, Kenji Fukuda, William S. Price, Yoji Arata
Summary: The study used high resolution MRI to investigate freezing behaviors in wintering Daphne kamtschatica var. jezoensis flower buds, revealing that anthers remained stable supercooled while ovules/pistils slowly dehydrated. Cryomicroscopic observation showed ice formation in calyx tubes and pistils.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Agronomy
Anatolii V. Pomortsev, Nikolay V. Dorofeev, Svetlana Yu. Zorina, Natalia B. Katysheva, Lada G. Sokolova, Anna S. Zhuravkova, Elena V. Mikhailova
Summary: Winter rye exhibits a higher adaptive capacity to abiotic and biotic stressors compared to other winter crops. Its resistance to adverse environmental factors and versatility make it a highly interesting crop.
Article
Engineering, Chemical
Hongtao Zhang, Khadije El Kadi, Mohamed I. Hassan Ali, Isam Janajreh
Summary: This study focuses on the experimental investigation of ice nucleation during the freezing of brine droplets. The effects of droplet size, salinity, and freezing temperature on nucleation behavior are examined. The results show that smaller droplets have faster cooling rates, reduced supercooling degrees, and quicker nucleation onset. Higher droplet salinity leads to decreased nucleation temperatures, while lower freezing temperatures enhance cooling rates and reduce nucleation times.
Article
Plant Sciences
Gabija Vaitkeviciute, Andrius Aleliunas, Yves Gibon, Rita Armoniene
Summary: The study found that higher concentrations of ascorbate were present in leaves, while crowns accumulated more nicotinamide adenine dinucleotide during cold acclimation, deacclimation, and reacclimation in winter wheat. Constant low temperature conditions during cold acclimation led to increased accumulation of ascorbate and glutathione in plant tissues, whereas prolonged higher low temperature conditions had a negative impact on freezing tolerance.
Article
Plant Sciences
Gabija Vaitkeviciute, Andrius Aleliunas, Yves Gibon, Rita Armoniene
Summary: Global climate change will result in longer and warmer autumns, which will negatively affect the quality of cold acclimation and reduce the freezing tolerance of winter wheat. Exposure to prolonged higher low-temperature induces higher shoot biomass accumulation but has a negative impact on winter wheat's freezing tolerance. Deacclimation leads to significantly reduced freezing tolerance, while reacclimation allows for the recovery of freezing tolerance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Entomology
Carl M. Stenoien, Lindsey Christianson, Kelton Welch, Jonathan Dregni, Keith R. Hopper, George E. Heimpel
Summary: Broad-spectrum insecticides are the main control measure for soybean aphid in North America, but biological control by natural enemies such as Aphelinus certus can also be effective. We tested the hypothesis that A. certus may experience high overwintering mortality. In the lab, we found that A. certus is freeze-intolerant and its survival decreases with longer durations of low temperature exposure.
BULLETIN OF ENTOMOLOGICAL RESEARCH
(2023)
Article
Entomology
Marton J. Paulin, Csaba B. Eoetvoes, Petr Zabransky, Gyoergy Csoka, Martin Schebeck
Summary: The North American oak lace bug is an invasive species in Europe and poses a serious threat to oak-dominated forests. This study examined the cold-tolerance strategy, supercooling points, and chilling-related mortality of overwintering adults to assess their ability to survive harsh winters. The results showed that C. arcuata is a freeze-avoidant species with moderate risk of mortality from chilling injuries.
AGRICULTURAL AND FOREST ENTOMOLOGY
(2023)
Article
Multidisciplinary Sciences
Nicolas R. Cecchetto, Susana M. Medina, Florencia Baudino, Nora R. Ibarguengoytia
Summary: In temperate, polar, and montane environments, ectotherms have developed physiological mechanisms to survive the coldest months of the year. Liolaemus lineomaculatus, a species of lizard, is able to tolerate freezing temperatures by altering their biochemical balance or supercooling. The analysis also showed that lizards from a colder site had a lower supercooling point and were able to survive below 0 degrees C temperatures.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2022)
Article
Engineering, Environmental
Sean Boyd, Tadros Ghobrial, Mark Loewen
Summary: Heat loss from the water surface is the key factor driving supercooling and ice formation in northern rivers. Accurately estimating the different heat components is crucial for modeling supercooling. Analysis of observed data and calculated surface heat budget revealed that longwave radiation was the dominant cooling heat flux, while shortwave radiation was the dominant warming heat flux.
COLD REGIONS SCIENCE AND TECHNOLOGY
(2023)
Article
Plant Sciences
Johanna Wagner, Karla Gruber, Ursula Ladinig, Othmar Buchner, Gilbert Neuner
Summary: Plants in the higher elevations of the European Alps may experience extremely low winter temperatures, which have a significant negative impact on the viability of flower buds in species like Saxifraga bryoides and Saxifraga moschata. Both short-time and long-time freezing at low temperatures lead to decreased flower bud viability and flowering frequency, suggesting damages are caused by freeze dehydration.
Article
Biochemistry & Molecular Biology
Jan Rozsypal, Martin Moos, Ivo Rudolf, Vladimir Kostal
Summary: The risks of energy reserve depletion and encountering low temperatures can limit the winter survival of mosquito populations. Autumn females have sufficient lipid reserves for overwintering, but high metabolic rates during flight may lead to rapid depletion of these reserves. The cold hardiness of females is weaker for above-ground habitats, but sufficient for overwintering in most underground spaces.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2021)
Article
Entomology
Petr Dolezal, Lenka Kleinova, Marketa Davidkova
Summary: The large pine weevil, Hylobius abietis, shows a preference for Seedlings of Pseudotsuga menziesii as a food source, but low oviposition rates indicate lack of nutrients. Betula pendula is the least preferred food source, causing high mortality among weevils. Different tree species have a significant impact on the fecundity of weevils.
Article
Biology
Bernard Y. Kim, Jeremy R. Wang, Danny E. Miller, Olga Barmina, Emily Delaney, Ammon Thompson, Aaron A. Comeault, David Peede, Emmanuel R. R. D'Agostino, Julianne Pelaez, Jessica M. Aguilar, Diler Haji, Teruyuki Matsunaga, Ellie E. Armstrong, Molly Zych, Yoshitaka Ogawa, Marina Stamenkovic-Radak, Mihailo Jelic, Marija Savic Veselinovic, Marija Tanaskovic, Pavle Eric, Jian-Jun Gao, Takehiro K. Katoh, Masanori J. Toda, Hideaki Watabe, Masayoshi Watada, Jeremy S. Davis, Leonie C. Moyle, Giulia Manoli, Enrico Bertolini, Vladimir Kostal, R. Scott Hawley, Aya Takahashi, Corbin D. Jones, Donald K. Price, Noah Whiteman, Artyom Kopp, Daniel R. Matute, Dmitri A. Petrov
Summary: Over 100 years of research on Drosophila melanogaster and related species have led to key discoveries in genetics, genomics, and evolution. Recent advances in long-read sequencing have enabled the generation of high-quality genome assemblies for tens or even hundreds of species. Utilizing Oxford Nanopore sequencing, a community resource of genome assemblies for 101 lines of 93 drosophilid species has been built, providing highly contiguous and complete genomes with high accuracy in coding regions.
Review
Entomology
Jan Rozsypal
Summary: This review discusses the molecular mechanisms of injury caused by low temperatures and freezing. It focuses on the damage to macromolecules such as proteins, nucleic acids, and biological membranes. The interaction between temperature effects, ice formation, and secondary effects such as osmotic stress, increased solute concentration, cellular freeze dehydration, disrupted ionic balance, and oxidative stress contribute to the damage. The review also identifies gaps in our knowledge of cold injury mechanisms and suggests future research directions.
EUROPEAN JOURNAL OF ENTOMOLOGY
(2022)
Article
Physiology
Zeljko D. Popovic, Vitezslav Maier, Milos Avramov, Iva Uzelac, Snezana Gosic-Dondo, Dusko Blagojevic, Vladimir Kostal
Summary: This study tracked the changes in energy metabolism of diapausing larvae under cold and warm acclimation conditions, finding that energy metabolism changes in the cold-acclimated group facilitate the development of cold hardiness, while the high metabolic activity in the warm-acclimated group may lead to premature mortality.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Martin Moos, Jaroslava Korbelova, Tomas Stetina, Stanislav Opekar, Petr Simek, Robert Grgac, Vladimir Kostal
Summary: Cold-acclimated insects accumulate cryoprotectants through assimilating amino acids from their diet and glycogen serves as the major source of glucose units for cryoprotectant synthesis.
Article
Biology
Lukas Kucera, Martin Moos, Tomas Stetina, Jaroslava Korbelova, Petr Vodrazka, Lauren Des Marteaux, Robert Grgac, Petr Hula, Jan Rozsypal, Milos Faltus, Petr Simek, Radislav Sedlacek, Vladimir Kostal
Summary: This study identifies and quantifies multiple cryoprotectants in larval tissues of a subarctic fly, which could inspire the development of laboratory cryoprotectant formulations for cryopreservation. The artificial mixtures of cryoprotectants mimic the concentrations in hemolymph of freeze-tolerant larvae and exhibit cryoprotective effects. The study also suggests that trehalose stimulates the transition to the amorphous glass phase, while proline forms a layer of dense viscoelastic liquid, both protecting macromolecules and cells from thermomechanical shocks associated with freezing.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2022)
Article
Biology
Petr Hula, Martin Moos, Lauren Des Marteaux, Petr Simek, Vladimir Kostal
Summary: This study reveals that exposing diapausing larvae of the drosophilid fly to dry conditions significantly improves their freeze tolerance. The metabolomics analysis shows that environmental drought, decreasing ambient temperatures, and short days trigger similar metabolic rearrangement and improved larval freeze tolerance. Transition to metabolic suppression represents a common axis of metabolic pathway reorganization towards accumulation of cytoprotective compounds and stimulation of freeze tolerance.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Robert Grgac, Jan Rozsypal, Lauren Des Marteaux, Tomas Stetinab, Vladimir Kostal
Summary: This study provides evidence for the molecular mechanisms of protein denaturation and membrane integrity loss in freeze-sensitive insects, and demonstrates the protective effects of cryoprotective molecules in stabilizing proteins and membranes against freezing injury.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Petr Dolezal, Marketa Davidkova, Petr Vovesny, Pavel Drasar
Summary: This paper discusses the practical experience with a new pheromone dispenser called Acumi-protect, aimed at addressing the occurrence of the sharp-dentated bark beetle (Ips acuminatus Glyl.), one of the most aggressive European bark beetle species in pine trees.
Article
Forestry
Marketa Davidkova, Lenka Kleinova, Petr Dolezal
Summary: The economic importance of the double-spined bark beetle, Ips duplicatus, has been increasing in many European countries. However, many aspects of its life cycle remain unknown, making its management challenging. This study found that the adult beetles primarily overwinter in forest litter, suggesting that removing infested trees during winter is ineffective. It is suggested that trees infested with adult I. duplicatus should be removed no later than September.
Review
Ecology
Daniela Hlavkova, Petr Dolezal
Summary: Climatic extremes have been intensifying since the 1880s and are believed to be a long-term factor contributing to increased mortality of Scots pine trees in Europe. Rising temperatures and precipitation deficiency are accelerating the impacts of weather on trees, while also fueling outbreaks of bark beetles, further exacerbating the situation.
FRONTIERS IN FORESTS AND GLOBAL CHANGE
(2022)