Article
Entomology
Colin A. R. Bonser, Carlos E. Astete, Cristina M. Sabliov, Jeffrey A. Davis
Summary: Previous research found that positively charged zein nanoparticles were toxic to neonates of Anticarsia gemmatalis Hubner and other noctuid pests, but the specific mechanisms were unknown. This study ruled out the hypotheses that toxic effects were caused by surfactant charges and antifeedant capabilities. It also found that both (+)ZNP and its cationic surfactant DDAB decreased antioxidant levels in A. gemmatalis neonates, suggesting inhibition of antioxidant levels. These findings contribute to the understanding of potential modes of action of biopolymeric nanoparticles.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Entomology
S. Ongaratto, C. M. Silveira, M. C. Santos, J. E. R. Gorri, M. M. P. Sartori, Thomas E. Hunt, A. L. Lourencao, E. L. L. Baldin
Summary: Injury by herbivores is a major stress for soybean production, with Anticarsia gemmatalis causing significant economic damage. Chemical control and Bt transgenic soybean are common management strategies, but alternative methods such as host plant resistance are considered more efficient. Antixenosis and antibiosis were found in several soybean genotypes, providing potential resistance to A. gemmatalis.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Neilier R. Silva-Junior, Yaremis M. Cabrera, Samuel L. Barbosa, Rafael de A. Barros, Edvaldo Barros, Camilo E. Vital, Humberto J. O. Ramos, Maria Goreti A. Oliveira
Summary: This study characterized the intestinal proteome of the lepidopteran Anticarsia gemmatalis, identifying multiple important isoforms of intestinal proteases and analyzing their interactions with inhibitors. The docking analysis revealed efficient binding between soybean PI and trypsin sequences without changes in the binding site sequence. Additionally, a SERPIN was identified as a potential regulator, providing support for the rational design of molecules with greater stability and resistance to protease cleavage for controlling insect pests like A. gemmatalis.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Agronomy
Rafael de Almeida Barros, Yaremis Merino-Cabrera, Camilo E. Vital, Neilier R. da Silva Junior, Caue N. de Oliveira, Samuel Lessa Barbosa, Joao Marques Goncalves Assis, Humberto J. O. Ramos, Maria G. de Almeida Oliveira
Summary: The results suggest that the peptides GORE1 and GORE2, designed to inhibit trypsin-like enzymes in Anticarsia gemmatalis, show potential as candidates for pest management and provide valuable information for the development of new inhibitors based on peptidomimetic tools.
PEST MANAGEMENT SCIENCE
(2021)
Article
Agronomy
Sabrina Ongaratto, Edson L. L. Baldin, Thomas E. Hunt, Debora G. Montezano, Emily A. Robinson, Maria C. dos Santos
Summary: The study found that the velvetbean caterpillar had a competitive advantage over the soybean looper, affecting the survival and behavior of the latter. Additional research is recommended to further understand the effects of interactions, especially in field conditions.
PEST MANAGEMENT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Larissa Forim Pezenti, Daniel Ricardo Sosa-Gomez, Rogerio Fernandes de Souza, Laurival Antonio Vilas-Boas, Katia Brumatti Goncalves, Carlos Roberto Maximiano da Silva, Gislayne Trindade Vilas-Boas, Adrivanio Baranoski, Mario Sergio Mantovani, Renata da Rosa
Summary: This study used RNA-seq to investigate the response of Anticarsia gemmatalis to Bt HD73, identifying a large number of differentially expressed genes in susceptible and resistant larvae, including immunity-related genes and proteases. These results provide insights for controlling agricultural pests.
Review
Plant Sciences
Maike Cosse, Thorsten Seidel
Summary: This article discusses the main proton pumps in plant cells and their role in regulating cytosolic pH, emphasizing the coordination of pump activity through 14-3-3 proteins, phosphorylation events, ion concentrations, and redox conditions to maintain cellular homeostasis.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Virology
Ana Parras-Jurado, Delia Munoz, Ines Beperet, Trevor Williams, Primitivo Caballero
Summary: Outbreaks of the major soybean pest, Anticarsia gemmatalis, can be controlled without synthetic insecticides using natural isolates of the Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV). However, the development of chemical insecticides and transgenic soybean has led to a decline in the use of AgMNPV-based products. This study aimed to select novel variants of AgMNPV with suitable insecticidal traits to complement existing active ingredients.
Article
Environmental Sciences
Barbara Monteiro de Castro E. Castro, Luis Carlos Martinez, Angelica Plata-Rueda, Marcus Alvarenga Soares, Carlos Frederico Wilcken, Antonio Jose Vinha Zanuncio, Muhammad Fiaz, Jose Cola Zanuncio, Jose Eduardo Serrao
Summary: The anthranilic diamide chlorantraniliprole is a systemic insecticide that affects ryanodine receptors. It is toxic to the velvetbean caterpillar Anticarsia gemmatalis, causing severe histopathological effects in the midgut, reduced respiratory rates, and altered behavioral responses.
Article
Cell Biology
Soon C. Heo, Jae C. You, Suhan Jung, Yu N. Kim, Sang-Hun Shin, Jae-Yeol Lee, Hyung J. Kim
Summary: This study reveals the important regulatory roles of Pim kinases in periodontal disease, particularly in osteoclastogenesis and bone resorption activity. Pim-2 acts as a major modulator by regulating the expression of the a3 isoform.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Ecology
Fabricio Oliveira Fernandes, Tamires Doroteo de Souza, Ariadne Costas Sanches, Nayma Pinto Dias, Janete Apparecida Desiderio, Ricardo Antonio Polanczyk
Summary: The study investigated the life table parameters of Anticarsia gemmatalis exposed to different concentrations of Bt-based bioinsecticide. Results indicated that higher sub-lethal concentrations had the most impact on larval and adult stages, while incubation period, pupal stage, and pre-pupal stage were unaffected. Additionally, changes in sex ratio were observed at certain concentrations.
Article
Plant Sciences
Veronica Aparecida Faustino, Angelica de Souza Gouveia, Flaviane Silva Coutinho, Neilier Rodrigues da Silva Junior, Rafael de Almeida Barros, Yaremis Merino Cabrera, Camilo Elber Vital, Virgilio A. Pereira Loriato, Laura Goncalves Costa Martins, Elizabeth Pacheco Batista Fontes, Humberto Josue de Oliveira Ramos, Maria Goreti Almeida Oliveira
Summary: Climate changes are causing increases in global temperatures, precipitation variability, and more frequent insect pest outbreaks. Research on plant genotypes has shown that drought-tolerant and insect-resistant varieties exhibit lower susceptibility to insect attacks, with different genotypes displaying varying levels of resistance.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Genetics & Heredity
Cintia N. Parsza, Diego L. M. Gomez, Jorge A. Simonin, Mariano Nicolas Belaich, Pablo D. Ghiringhelli
Summary: The study demonstrated that the budded virions of Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) can transduce and support the expression of delivered genes in mammalian cells but do not replicate. Therefore, AgMNPV has the potential to be used as an alternative to non-infectious viruses in humans for exploring new approaches in gene therapy and other applications based on the use of mammalian cells.
CURRENT GENE THERAPY
(2021)
Article
Multidisciplinary Sciences
Sonja Michele Schmidtpott, Thorsten Seidel
Summary: This study presents a simple and reliable protocol for FRET measurements, including procedures for protein expression estimation and adjustment of laser and detector settings. Data evaluation will be conducted through calibration with a fluorescent fusion protein of known FRET efficiency.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Cell Biology
Michelle E. Maxson, Yazan M. Abbas, Jing Ze Wu, Jonathan D. Plumb, Sergio Grinstein, John L. Rubinstein
Summary: This study shows that proton-pumping vacuolar H+ ATPases (V-ATPases) are responsible for the acidic environment in secretory and endocytic organelles. The researchers developed a new probe derived from SidK protein to visualize and quantify V-ATPases with high specificity. They found that the density of V-ATPases increases during phagosome maturation and their distribution varies in lysosomes depending on their subcellular localization.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Microbiology
Jhon Jhamilton Artunduaga Bonilla, Leandro Honorato, Krupanandan Haranahalli, Isabella Dib Ferreira Gremiao, Sandro Antonio Pereira, Allan Guimaraes, Andrea Regina de Souza Baptista, Patricia de M. Tavares, Marcio L. Rodrigues, Kildare Miranda, Iwao Ojima, Maurizio Del Poeta, Leonardo Nimrichter
Summary: Sporotrichosis is an emerging mycosis caused by Sporothrix genus fungi, particularly affecting humans and cats. Treatment options for feline sporotrichosis, such as ITC, potassium iodide, and amphotericin B, can have deleterious adverse reactions. Acylhydrazone derivatives like D13 and SB-AF-1002 show potent antifungal activity with low toxicity, displaying additive effects with ITC. In a murine model, AH derivatives are more efficient than ITC, suggesting their potential in combating sporotrichosis.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Kesley A. O. Pontes, Leandro S. Silva, Edgleyson C. Santos, Alessandro S. Pinheiro, Douglas E. Teixeira, Diogo B. Peruchetti, Rodrigo P. Silva-Aguiar, Camila H. C. Wendt, Kildare R. Miranda, Andrelina N. Coelho-de-Souza, Jose Henrique Leal-Cardoso, Celso Caruso-Neves, Ana Acacia S. Pinheiro
Summary: The study demonstrated the potential anti-malarial effect of eugenol against Plasmodium sp., reducing parasitemia and cerebral edema. By affecting the development of the erythrocytic cycle and inducing morphological changes, eugenol showed promise as a therapeutic strategy for malaria treatment.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Cell Biology
Lucio Ayres Caldas, Fabiana Avila Carneiro, Fabio Luis Monteiro, Ingrid Augusto, Luiza Mendonca Higa, Kildare Miranda, Amilcar Tanuri, Wanderley de Souza
Summary: This study used transmission electron microscopy and electron tomography to investigate the structural alterations in mammalian cells infected with SARS-CoV-2. The findings included different membranous structures derived from the endoplasmic reticulum, virus assembly through membrane budding, annulate lamellae in the cytoplasm, and virus particles in the perinuclear space. This contributes to a better understanding of the cell biology of SARS-CoV-2 and the mechanisms of virus-host cell interaction.
BIOLOGY OF THE CELL
(2021)
Article
Energy & Fuels
Youssef Bacila Sade, Camila Silva Goncalves, Sandra Mara Naressi Scapin, Guilherme Luiz Pinheiro, Roberto Becht Flatschart, Wanderley de Souza, Norton Heise, Ednildo de Alcantara Machado
Summary: This study reports the identification and characterization of a novel endoglucanase AfEG66 from the snail Achatina fulica, which shows high activity and stability against polysaccharide substrates, contributing to the improvement of production efficiency of green fuels.
BIOENERGY RESEARCH
(2022)
Article
Microbiology
Maira Turiel-Silva, Camila Wendt, Edilene O. Silva, Ana Paula Drummond Rodrigues, Wanderley de Souza, Kildare Miranda, Jose A. P. Diniz
Summary: This study utilized electron microscopy and tomography to reconstruct the three-dimensional structure of C. lignieresi-infected red blood cells, revealing the remodeling of erythrocytes induced by the parasite and providing new insights into its parasitic mechanism.
JOURNAL OF EUKARYOTIC MICROBIOLOGY
(2022)
Article
Plant Sciences
Cuc Thi Nguyen, Tatsuhiro Ezawa, Katsuharu Saito
Summary: This study conducted a comprehensive survey of VTC proteins in eight AM fungal genomes and characterized the biochemical properties of the Rhizophagus irregularis VTC4. The results suggest that AM fungal VTC4 not only synthesizes polyP but also regenerates ATP from polyP and ADP.
SOIL SCIENCE AND PLANT NUTRITION
(2022)
Article
Biology
Sachiko Tanaka, Kayo Hashimoto, Yuuki Kobayashi, Koji Yano, Taro Maeda, Hiromu Kameoka, Tatsuhiro Ezawa, Katsuharu Saito, Kohki Akiyama, Masayoshi Kawaguchi
Summary: This study investigates the effects of two plant hormones on the growth and sporulation of the arbuscular mycorrhizal fungus, Rhizophagus clarus. The results demonstrate that these hormones can induce the production of a large number of spores and promote the growth of host plants.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jarlene A. Lopes, Federica Collino, Clara Rodrigues-Ferreira, Luzia da Silva Sampaio, Gloria Costa-Sarmento, Camila H. C. Wendt, Fernando P. Almeida, Kildare R. Miranda, Tais H. Kasai-Brunswick, Rafael S. Lindoso, Adalberto Vieyra
Summary: This study investigated whether extracellular vesicles secreted by adipose tissue mesenchymal cells (EVs) can suppress the excessive production of mitochondrial O-2(center dot-) caused by acute kidney injury (AKI). The results showed that administration of EVs can decrease mitochondrial O-2(center dot-) formation and promote the recovery of renal structures and functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Edgleyson C. dos Santos, Leandro S. Silva, Alessandro S. Pinheiro, Douglas E. Teixeira, Diogo B. Peruchetti, Rodrigo P. Silva-Aguiar, Camila H. C. Wendt, Kildare R. Miranda, Andrelina N. Coelho-de-Souza, Jose Henrique Leal-Cardoso, Celso Caruso-Neves, Ana Acacia S. Pinheiro
Summary: This study demonstrates the potential anti-malarial effect of 1,8-cineole, which reduces parasitemia and has an impact on the erythrocytic cycle of the parasite.
Review
Microbiology
Veronica Jimenez, Kildare Miranda, Ingrid Augusto
Summary: Osmoregulation is a crucial cellular process for the survival of all organisms, and it holds even more significance for protists whose life cycles occur in various environments. This article summarizes the current understanding of the osmoregulatory mechanisms in the contractile vacuole and explores its newly discovered functions in cell trafficking and signaling.
JOURNAL OF EUKARYOTIC MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Juliana Maira Freitas Vieira, Laura Nicoleti Zamproni, Camila H. C. Wendt, Kildare Rocha de Miranda, Rafael Soares Lindoso, Sang Won Han
Summary: This study demonstrates that the therapeutic properties of mesenchymal stromal cell (MSC)-derived extracellular vesicles (EVs) can be improved by enriching them with specific miRNAs through lentiviral vector modification. The overexpression of miR-210-5p in EVs significantly promotes tubular structure formation in human umbilical vein endothelial cells (HUVECs), leading to increased expression of angiogenic proteins and genes. This highlights the potential of manipulating EV cargo to enhance specific angiogenic potential.
Article
Physiology
Gustavo Monnerat, Tais Hanae Kasai-Brunswick, Karina Dutra Asensi, Danubia Silva dos Santos, Raiana Andrade Quintanilha Barbosa, Fernanda Cristina Paccola Mesquita, Joao Paulo Calvancanti Albuquerque, Pires Ferreira Raphaela, Camila Wendt, Kildare Miranda, Gilberto Barbosa Domont, Fabio Cesar Sousa Nogueira, Adriana Bastos Carvalho, Antonio Carlos Campos de Carvalho
Summary: This study modeled Hutchinson-Gilford Progeria Syndrome using cardiomyocytes derived from induced pluripotent stem cells and found significant changes in mitochondrial morphology and protein expression, suggesting novel mechanisms underlying premature cardiac aging.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Wendell Girard-Dias, Ingrid Augusto, Tacio V. A. Fernandes, Pedro G. Pascutti, Wanderley de Souza, Kildare Miranda
Summary: In this study, the three-dimensional distribution of diffusible elements within acidocalcisomes in Trypanosoma cruzi was mapped using advanced electron microscopy techniques. The existence of nanodomains with specific element distribution patterns was discovered. These findings have significant implications for understanding nanophysiology of organelles.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Anna L. S. Barreto, Ariadne N. Alonso, Daniel C. De Moraes, Jose A. R. Curvelo, Kildare Miranda, Maristela B. Portela, Antonio Ferreira-Pereira, Thais Souto-Padron, Rosangela Maria De A. Soares
Summary: The search for new therapeutic strategies for leishmaniasis treatment is essential. The aqueous extract of the marine sponge Dercitus (Stoeba) latex displayed antileishmanial activity and moderate toxicity against peritonial macrophages. This study suggests that Dercitus (Stoeba) latex may be a potential source of drugs for the treatment of L. amazonensis infections.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2022)
Article
Nanoscience & Nanotechnology
Jhon Jhamilton Artunduaga Bonilla, Leandro Honorato, Allan J. Guimaraes, Kildare Miranda, Leonardo Nimrichter
Summary: This research focuses on the green synthesis of chitosan-functionalized silver nanoparticles (AgNPs@Chi) and their potential applications in combating sporotrichosis. The results demonstrate that AgNPs@Chi exhibit low toxicity, inhibitory effects on fungal growth, and stimulating effects on cell growth and tissue repair. These nanocomposites have the potential to be a non-toxic and efficient alternative for sporotrichosis treatment.
FRONTIERS IN NANOTECHNOLOGY
(2022)