Article
Chemistry, Physical
Lan Zhang, Christophe V. F. P. Laurent, Lorenz Schwaiger, Lushan Wang, Su Ma, Roland Ludwig
Summary: Direct bioelectrocatalysis involves efficient electron transfer between enzymes and electrodes without the use of redox mediators, and has applications in biosensors, biofuel cells, and bioelectrosynthesis. Cellobiose dehydrogenase (CDH) is a well-studied multidomain bioelectrocatalyst with a catalytic flavodehydrogenase domain and a mobile, electron-transferring cytochrome domain linked by a flexible linker. This study investigates the regulatory mechanism of the linker on electron transfer in CDH and proposes a function-based definition of the linker region. The findings provide insights into optimizing electron transfer rates in multidomain enzymes and enhancing their bioelectrocatalytic performance through rational design of the interdomain linker.
Article
Chemistry, Physical
C. Felipe Garibello, Alexandr N. Simonov, Daniel S. Eldridge, Francois Malherbe, Rosalie K. Hocking
Summary: This study investigated the relationship between direct reduction and catalytic reduction of iron sulfides. It was found that amorphous iron sulfide showed the highest selectivity, acting as both a direct reductant and a catalyst.
Article
Chemistry, Physical
Sulekha Sharma, Saina Shaheeda, Kundan Shaw, Alakesh Bisai, Amit Paul
Summary: Dimerized 3-substituted 2-oxindoles were synthesized using TEMPO as a redox mediator and TEMPO+ as an in situ generated electrocatalyst. Two-electron and one-electron transfer pathways were observed depending on the oxidation potentials of the 2-oxindoles. This study also demonstrated the use of electron-transfer rate measurement, prevention of electrode passivation, electrolyte recovery, and mild reaction conditions in greener electro-organic synthesis. Finally, an efficient electrochemical oxidation strategy was employed for the total synthesis of two dimeric alkaloids.
Article
Chemistry, Multidisciplinary
Biswajit Mondal, Soumitra Dinda, Neeta Karjule, Sanjit Mondal, Alagar Raja Kottaichamy, Michael Volokh, Menny Shalom
Summary: Electrolysis of water is a sustainable method to produce clean hydrogen, but it requires a high applied potential due to the pH-dependency of the hydrogen and oxygen evolution reactions. By combining proton-coupled electron transfer and single-electron transfer processes, we have achieved hydrogen production from water at low cell potentials by pH adjustment.
Article
Chemistry, Physical
Alexander M. Khenkin, Adi Herman, Eynat Haviv, Ronny Neumann
Summary: The study demonstrates the room-temperature electrocatalytic oxyesterification of alkanes and benzene with Pb-IV(TFA)(4) as catalysts. The results show high yields and efficiencies, marking an important advancement towards realizing selective alkane and arene oxidation reactions.
Review
Chemistry, Applied
Jing Du, Yuan-Yuan Ma, Huaqiao Tan, Zhen-Hui Kang, Yangguang Li
Summary: Electrochemical CO2 reduction to value-added fuels and chemicals is an effective strategy due to its high efficiency and economic feasibility. Polyoxometalates (POMs) are promising candidates for efficient CO2RR electrocatalysts and play significant roles in the process and performance of catalytic CO2RR.
CHINESE JOURNAL OF CATALYSIS
(2021)
Article
Engineering, Environmental
Jahidul Hoq, Md. Fahamidul Islam, Md. Rezwan Miah, Mohammed M. Rahman, Albandary Almahri, Mohammad A. Hasnat
Summary: In this study, the electrochemical oxidation of paracetamol was investigated using a polycrystalline gold electrode modified with an iodine adlayer. The iodide adsorption on the electrode surface eliminated the limitation of PCT adsorption and improved the PCT oxidation reaction. The formulated iodine adlayer electrode showed a diffusion-controlled pathway for PCT electro-oxidation with a high reaction rate. The electrode also exhibited good sensitivity and low detection limit for PCT detection.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Review
Biotechnology & Applied Microbiology
Likun Luan, Xiuling Ji, Boxia Guo, Jinde Cai, Wanrong Dong, Yuhong Huang, Suojiang Zhang
Summary: Activation and conversion of CO2 into high-value-added products have become a promising approach to address environmental issues. This review comprehensively discusses the integration of enzymatic-electrocatalysis and microbial-electrocatalysis technologies for CO2 reduction, focusing on the electron transfer routes and the compatibility of electrode materials. The combination of solar energy-driven photo-electrochemical techniques with biocatalysis is also explored. The significant findings and future prospects of green and sustainable bioelectrocatalysis for CO2 reduction are highlighted, providing a blueprint for the production of valuable products from CO2 under efficient and mild conditions.
BIOTECHNOLOGY ADVANCES
(2023)
Editorial Material
Biochemistry & Molecular Biology
F. Peter Guengerich
Summary: The paper introduces the concept of cytochrome b(5) as an auxiliary protein facilitating cytochrome P450 reactions, providing important insights for future research.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Materials Science, Multidisciplinary
Fan Yang, Yurui Gao, Pengcheng Zhao, Yuan He, Yuqiao Wang
Summary: In this study, 1D ZnO@C@MoS(2) nanoarrays on conducting glass were fabricated as an effective counter electrode for dye-sensitized solar cells. The power conversion efficiency reached 3.61% due to enhanced electron transfer and electrocatalytic activity.
Article
Chemistry, Multidisciplinary
Ke Guo, Dongping Fan, Jianchun Bao, Yafei Li, Dongdong Xu
Summary: In this study, atomic-level P-doped Pt nanodendrites with tunable composition and highly branched architecture were successfully prepared, showing significantly improved electrocatalytic performance compared to commercial Pt/C, especially in terms of stability.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Xiu Lin, Zhaoyu Zhou, Qi-Yuan Li, Dong Xu, Si-Yuan Xia, Bing-Liang Leng, Guang-Yao Zhai, Shi-Nan Zhang, Lu-Han Sun, Guohua Zhao, Jie-Sheng Chen, Xin-Hao Li
Summary: In this study, the highly selective epoxidation of cyclooctene was successfully improved using electron-rich RuO2 nanocrystals through direct oxygen transfer from water under mild conditions. The electron-rich RuO2 electrocatalysts enable efficient and durable hydrogen production without impacting the selectivity of the reaction.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Niklas von Wolff, Marc Robert
Summary: Electron serves as the ultimate redox reagent for building and reshaping molecular structures. Understanding and controlling the parameters involved in dissociative electron transfer (DET) reactivity and its coupling with proton transfer are crucial for enhancing selectivity, kinetics, and energy efficiency in molecular chemistry. Various applications, such as photoredox catalysis, CO2 reduction, and alcohol oxidation, highlight the broad range of processes encompassed by DET pathways and demonstrate the importance of studying the driving force-rate relationships for future developments in energy efficient catalytic schemes in redox organic chemistry.
Review
Chemistry, Applied
Lin Tian, Zhao Li, Peng Wang, Xiuhui Zhai, Xiang Wang, Tongxiang Li
Summary: Zero-dimensional carbon quantum dots have drawn increasing attention for their unique properties such as low cost, large surface area, high electrical conductivity, and rich functional groups. They show promise as functional materials in energy conversion through electrocatalysis, enhancing conductivity and optimizing electronic structure.
JOURNAL OF ENERGY CHEMISTRY
(2021)
Article
Chemistry, Applied
Xueqing Gao, Xiaomeng Liu, Shujiao Yang, Wei Zhang, Haiping Lin, Rui Cao
Summary: This study reports a biology-inspired hybrid electrocatalyst that mimics the functions in natural photosynthesis, demonstrating outstanding performance in the oxygen evolution reaction. The catalyst achieves efficient oxygen evolution by constructing specialized channels for proton and electron transfer.
CHINESE JOURNAL OF CATALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Guobing Wei, Wenxing Zhang, Hanfeng Cui, Fusheng Liao, Lin Cheng, Guangqiang Ma, Hao Fan, Nian Hong, Jing Zhang
Summary: A novel electrochemical DNA sensor utilizing catalytic hairpin assembly (CHA) and tetraferrocene as a signal marker was developed to achieve signal amplification efficiency. The sensor showed good linear correlation and low detection limit under optimal conditions, with excellent selectivity, reproducibility, and stability.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Chemistry, Applied
Elisa Vignali, Loredano Pollegioni, Giovanna Di Nardo, Francesca Valetti, Silvia Gazzola, Gianfranco Gilardi, Elena Rosini
Summary: This study reported the first multi-enzymatic, one-pot bioconversion process of vanillin into ccMA, achieving high yield in a lab-scale procedure within 30 hours. This research demonstrates a sustainable alternative for producing high value added products from renewable resources.
ADVANCED SYNTHESIS & CATALYSIS
(2022)
Article
Environmental Sciences
Marta Gea, Chao Zhang, Roberta Tota, Gianfranco Gilardi, Giovanna Di Nardo, Tiziana Schiliro
Summary: The study evaluated the interference of five pesticides on estrogen biosynthesis and estrogen signaling, finding that while the pesticides did not impact aromatase activity, Methiocarb was able to alter estrogen signaling at high doses. This suggests that Methiocarb should be considered a potential endocrine disruptor.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Gianluca Catucci, Alberto Ciaramella, Giovanna Di Nardo, Chao Zhang, Silvia Castrignano, Gianfranco Gilardi
Summary: This study further characterized the 3A4-BMR chimera using differential scanning calorimetry and evaluated the stabilizing role of BMR. The fusion to BMR was found to have a significant effect on thermal stability but a minimal effect on catalytic activity. Different linker loop lengths had an impact on both activity and stability of the chimera, highlighting the importance of careful design and evaluation of P450-BMR chimeras.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Antonino Famulari, Danilo Correddu, Giovanna Di Nardo, Gianfranco Gilardi, Mario Chiesa, Ines Garcia-Rubio
Summary: This study characterized the coordination environment of iron in the isolated CYP116B5 heme domain using continuous wave and advanced EPR techniques. It found that the electronic ground state of CYP116B5hd is similar to classical P450 monooxygenases rather than peroxygenases. The detection of nitrogen-mediated axial coordination explains the similarity to peroxygenases. The findings contribute to the understanding of the distinguishing features between classical P450 enzymes and peroxygenases.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Antonino Famulari, Danilo Correddu, Giovanna Di Nardo, Gianfranco Gilardi, Mario Chiesa, Ines Garcia-Rubio
Summary: This paper documents a dataset obtained from an Electron Paramagnetic Resonance (EPR) study on the electronic properties of a self-sufficient cytochrome P450, CYP116B5hd, which has potential catalytic activity for synthetic purposes. The paper provides raw data, supplementary data, simulation scripts, and detailed descriptions of experimental procedures and instrumental details.
Article
Biochemistry & Molecular Biology
Chao Zhang, Gianfranco Gilardi, Giovanna Di Nardo
Summary: This study reports the capture and spectroscopic characterization of compound I (Cpd I) of human aromatase, highlighting its importance in aromatization reaction. Mutagenesis experiments confirm the involvement of specific residues in proton delivery for Cpd I generation. The data provide new evidences on the role of threonine in oxygen activation in cytochromes P450.
Article
Biotechnology & Applied Microbiology
Gianluca Catucci, Simone Turella, Hanna Cheropkina, Melissa De Angelis, Gianfranco Gilardi, Sheila J. Sadeghi
Summary: In this study, an enzyme with an active site mutation was used to transform indole into indigo or indirubin, providing a green alternative for the production of indigoids.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Daniele Giuriato, Danilo Correddu, Gianluca Catucci, Giovanna Di Nardo, Cristiano Bolchi, Marco Pallavicini, Gianfranco Gilardi
Summary: This study designed an enzyme capable of self-producing hydrogen peroxide (H2O2) and applied it to industrial biocatalysis, effectively solving the toxicity issue of exogenous H2O2 in catalytic reactions.
Article
Biochemistry & Molecular Biology
Hanna Cheropkina, Gianluca Catucci, Federico Cesano, Arianna Marucco, Gianfranco Gilardi, Sheila J. Sadeghi
Summary: It is advantageous to develop an in vitro platform for predicting the complexity of in vivo systems. The initial step involves identifying a xenobiotic that undergoes monooxygenation through two sequential enzymatic reactions. Pesticides, specifically metabolized by human flavin-containing monooxygenase 1 (hFMO1) and cytochrome P450 (CYP), serve as a suitable model for tandem reactions. By immobilizing hFMO1 on glassy carbon electrodes modified with graphene oxide (GO) and cationic surfactant didecyldimethylammonium bromide (DDAB), the feasibility of the in vitro platform is demonstrated. The bioelectrode successfully catalyzes the expected sulfoxide products of three pesticides and exhibits the ability to mimic complex metabolic reactions of xenobiotics within the human body when tested in a tandem system with hFMO1 and CYP3A4.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Danilo Correddu, Sabrina Helmy Aly, Giovanna Di Nardo, Gianluca Catucci, Cristina Prandi, Marco Blangetti, Chiara Bellomo, Elisabetta Bonometti, Guido Viscardi, Gianfranco Gilardi
Summary: This study found that the cytochrome P450 BM3 mutants have enhanced and specific epoxidation activity for geraniol and linalool, forming valuable oxidized derivatives, highlighting the significance of these enzymes for industrial applications.
Article
Biochemistry & Molecular Biology
Michael Ramek, Jasmina Sabolovic
Summary: This paper investigates the geometries, energy landscapes, and magnetic parameters of copper(II) amino acid compounds using density functional theory calculations. The results show that certain conformers with histaminate-like and glycine-like modes have low Gibbs free energies and high metal-binding affinities. The predicted conformations reproduce the experimental electron paramagnetic resonance parameters.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Wei Tian, Wen Zhong, Zengyan Yang, Ling Chen, Shijie Lin, Yanping Li, Yuxing Wang, Peilin Yang, Xing Long
Summary: A series of novel dibutyltin complexes based on salen-like ligands were synthesized and characterized. Complex S03 showed excellent in vitro anticancer activity and induced cancer cell death through multiple mechanisms. This study reveals new mechanisms of organotin complexes and provides new insights into the development of organotin metal complexes as anticancer drugs.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Natalie C. Korkola, Martin J. Stillman
Summary: Many proteins require a metal cofactor for their function, and these metals play a role in protein folding. The study found that the metallation rates of different metal binding pathways are influenced by pH, with the less cooperative pathway being more affected by low pH conditions. Specific mixtures of structures are formed in the initial steps of metallation, and unfolding the disordered apo-MT structure hinders the formation of these preliminary structures. The compact conformation of the native apo-MT allows for rapid formation of metal-thiolate structures with high affinity, providing protection from oxidation.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Boxuan Yang, Xitong Song, Binju Wang
Summary: The diiron active site plays a crucial role in catalytic transformations in both biological and chemical systems. Recent advancements in the field include the synthesis of biomimetic diiron catalysts inspired by the active structure of soluble methane monooxygenase (sMMO), which have been successfully applied to the dehydrogenation of indolines.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Christina N. Banti, Angeliki A. Piperoudi, Catherine P. Raptopoulou, Vassilis Psycharis, Constantinos M. Athanassopoulos, Sotiris K. Hadjikakou
Summary: Two copper(I) polymorphs with mitochondria-targeting properties were successfully prepared via metal ion conjugation. These compounds showed promising activities in DNA binding, enzyme inhibition, and cancer cell inhibition. The molecular mechanisms of action were investigated.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2024)