期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 108, 期 -, 页码 115-122出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2011.11.010
关键词
Scanning electrochemical microscopy; Reactive oxygen species; Human bladder cancer cells; Cisplatin
资金
- NSERC
- CIPI
- OPC
- CFI
- OIT
- PREA
- UWO
- China Scholarship Council
Reactive oxygen species (ROS) have gained great interests as they are closely related to cellular homeostasis and functions. New insights into physiology of cancer cells are anticipated from the analysis of their ROS evolution at single cell level. We discovered a periodical ROS evolution of human bladder cancer (124) cells by time-lapse scanning electrochemical microscopy (SECM). We define one ROS generation cycle consists of one active stage when the cell is releasing ROS and one resting stage when the cell is not releasing ROS. Quantitative study of the extracellular ROS profile of a 124 cell in different stages was accomplished by comparison of experimental and simulated probe approach curves. In the active stage, the distribution of ROS around an untreated 124 cell was found to be steady with a long cycle of alternation. When apoptosis is triggered by cisplatin, the periodicity of the ROS generation cycle is significantly accelerated. Enhanced ROS productivity was observed with cisplatin-treated 124 cells in the active stage. With ROS released from the cells as the redox mediator, SECM provides an excellent label-free method to monitor the physiological activities of single cancer cells. (c) 2011 Elsevier Inc. All rights reserved.
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