期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 103, 期 1, 页码 28-34出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2008.08.014
关键词
Highly-reactive oxygen species; Ferryl species; Terephthalate; Stopped-flow kinetics
资金
- Fonds zur Forderung der Wissenschaftlichen Forschung in Osterreich [19335-N17]
- Hochschuljubilaumstiftung der Stadt Wien [H-01684/2007]
- Science Foundation Ireland
- ECRF
- Fondazione MPS (Siena, Italy)
- ERAB (Brussels, Belgium)
The Fenton reaction was investigated, in a medium approximating to that of the extracellular fluid (ECF), by rapid-mixing stopped flow experiments and HPLC analysis using sodium terephthalate (TA(2-)). The reactive intermediate of the Fenton reaction hydroxylates the essentially nonfluorescent, TA(2-) to the brilliant fluorophor 2-hydroxy-terephthalate (OH-TA), which allows the Fenton reaction to be monitored in stopped-flow experiments. There was no artefactual quenching of the fluorescence by substances present in the Fenton-reaction mixture or in the artificial cerebrospinal fluid (aCSF) that might have influenced OH-TA quantification. A mathematical model based on kinetic considerations was developed. This explains the observed independence of the OH-TA concentration on the amount of TA(2-) present in aCSF as well as its dependence on TA(2-) concentration in potassium acetate buffer. A mechanism based on this model, involving complex formation between Fe(II), TA(2-) and H2O2, followed by an intra-molecular hydroxylation accompanied by an intra-molecular electron transfer was developed. The results are consistent with a reactive intermediate, which causes oxidative stress in vivo, not being a free hydroxyl radical, but a ferryl species or a crypto radical. The biological implications of these results are discussed. (c) 2008 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据