Article
Biology
Samy Dufour, Pascale Tacnet-Delorme, Jean-Philippe Kleman, Oleksandr Glushonkov, Nicole Thielens, Dominique Bourgeois, Philippe Frachet
Summary: CD47 serves as a "don't eat-me" signal to protect viable cells from phagocytosis by macrophages. Apoptosis induces changes in the plasma membrane, which leads to exposure of "eat-me" signals such as phosphatidylserine and calreticulin. This study uses imaging techniques to investigate how the distribution of these molecules on the cell surface correlates with plasma membrane alteration, SIRP alpha binding, and cell engulfment.
COMMUNICATIONS BIOLOGY
(2023)
Article
Environmental Sciences
Miki Kuroiwa, Shin-Ichiro Yamaguchi, Yoshinobu Kato, Arisa Hori, Saori Toyoura, Mai Nakahara, Nobuyuki Morimoto, Masafumi Nakayama
Summary: Our study demonstrates that the receptor Tim4 mediates the interaction between macrophages and microplastics through aromatic-aromatic interactions. It is involved in the engulfment of PS microplastics and MWCNTs, and disrupts the process of efferocytosis. These findings suggest that chronic exposure to large amounts of PS microplastics may lead to chronic inflammation and autoimmune diseases.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biology
Omar Pena-Ramos, Lucia Chiao, Xianghua Liu, Xiaomeng Yu, Tianyou Yao, Henry He, Zheng Zhou
Summary: Autophagosomes play a crucial role in facilitating the degradation of apoptotic cells. They are recruited to the surfaces of phagosomes containing apoptotic cells and subsequently fuse with them, allowing the inner vesicle to enter the phagosomal lumen. Defects in autophagosome production result in significant defects in the degradation of apoptotic cells.
Article
Cell Biology
Susumin Yang, Chanhyuk Min, Hyunji Moon, Byeongjin Moon, Juyeon Lee, Jaeseon Jeon, Hagyeong Kwon, Deokyun Jang, Daeho Park
Summary: The process of engulfing apoptotic cells, known as efferocytosis, requires both intra- and extracellular calcium. The mechanism of calcium flux during efferocytosis is highly regulated, and it leads to an increase in intracellular calcium levels. However, the exact role of elevated intracellular calcium during efferocytosis is still unknown.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Ok-Hee Kim, Geun-Hyung Kang, June Hur, Jinwook Lee, YunJae Jung, In-Sun Hong, Hookeun Lee, Seung-Yong Seo, Dae Ho Lee, Cheol Soon Lee, In-Kyu Lee, Susan Bonner-Weir, Jongsoon Lee, Young Joo Park, Hyeonjin Kim, Steven E. Shoelson, Byung-Chul Oh
Summary: Using unbiased proteomics, this study identified externalized phosphatidylinositides as apoptotic eat-me signals recognized by CD14(+) phagocytes.
CELL DEATH AND DIFFERENTIATION
(2022)
Review
Cell Biology
Yoshitaka Furuta, Zheng Zhou
Summary: Phosphatidylserine (PS) is asymmetrically distributed in the plasma membrane, with inner leaflet placement in live cells and exposure on outer surfaces in apoptotic cells. This exposed PS acts as an "eat-me signal" to attract neighboring engulfing cells. This article will review the mechanisms regulating PS exposure in necrotic and apoptotic cells, highlighting their similarities and differences.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Bruna Romana-Souza, Lin Chen, Trevor R. Leonardo, Zhenlong Chen, Luisa A. DiPietro
Summary: The study shows that fibroblasts can engulf apoptotic endothelial cells (ApoEC) and acquire a pro-healing phenotype in the process. The uptake of ApoEC is partly mediated by the milk fat globule-EGF factor 8 protein/integrin alpha(v)beta(5) pathway, which enhances cell migration, contractility, alpha-smooth muscle actin expression, and collagen deposition in fibroblasts.
Article
Cell Biology
Yu-Chieh Lin, Wen-Hui Tsai, Shao-Chi Chang, Hui-Chi Hsu
Summary: Membranous CD14 plays a crucial role in the phagocytic activity of neutrophils. This study investigates the role of CD14(+) microparticles derived from apoptotic neutrophils during the phagocytic process. It is found that CD14(+)apo-MP derived from apoptotic ATRA-NB4 cells promotes the phagocytic activity of viable ATRA-NB4 cells.
Article
Multidisciplinary Sciences
Jozef Pisko, Alexandra Spirkova, Stefan Cikos, Lucia Olexikova, Veronika Kovarikova, Zuzana Sefcikova, Dusan Fabian
Summary: The study demonstrates that embryonic cells possess the necessary mechanisms for recognizing, engulfing, and digesting apoptotic cells, ensuring the clearance of most dying blastomeres.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Marta T. Gomes, Karol Palasiewicz, Varsha Gadiyar, Kevin Lahey, David Calianese, Raymond B. Birge, David S. Ucker
Summary: This study investigates the surface determinants involved in triggering innate apoptotic immunity (IAI) and finds that the externalization of phosphatidylserine is not sufficient to trigger profound immunomodulatory effects of IAI, indicating the dissociation of apoptotic immunomodulation and phagocytosis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Editorial Material
Cell Biology
Zheng Zhou, Omar Pena-Ramos
Summary: During an animal's life, cells undergo programmed cell death called apoptosis. These cells are engulfed by other cells through phagocytosis and degraded inside phagosomes. Recent research has shown that the fusion of autophagosomes and phagosomes contributes to the degradation of apoptotic cells.
Article
Cell Biology
Chenxi Zheng, Bingdong Sui, Xiao Zhang, Jiachen Hu, Ji Chen, Jin Liu, Di Wu, Qingyuan Ye, Lei Xiang, Xinyu Qiu, Siying Liu, Zhihong Deng, Jun Zhou, Shiyu Liu, Songtao Shi, Yan Jin
Summary: The study revealed that apoptotic vesicles (apoVs) derived from MSCs can modulate liver macrophage homeostasis and counteract type 2 diabetes (T2D) by inducing macrophage reprogramming. Through efferocytosis, apoVs inhibit macrophage accumulation and promote their transformation towards an anti-inflammatory phenotype in the liver to ameliorate T2D phenotypes.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Biochemistry & Molecular Biology
Sho Morioka, Daiki Kajioka, Yusuke Yamaoka, Rochelle M. Ellison, Turan Tufan, Inge L. Werkman, Shinji Tanaka, Brady Barron, Satoshi T. Ito, Sarah Kucenas, Mark D. Okusa, Kodi S. Ravichandran
Summary: Defects in efferocytosis are linked to inflammatory diseases. A strategy called chimeric receptor for efferocytosis (CHEF) is designed to boost efferocytosis and reduce inflammation.
Article
Biochemistry & Molecular Biology
Bianca Petrignani, Samuel Rommelaere, Ketty Hakim-Mishnaevski, Florent Masson, Elodie Ramond, Reut Hilu-Dadia, Mickael Poidevin, Shu Kondo, Estee Kurant, Bruno Lemaitre
Summary: This study reveals the crucial role of the Nimrod family member NimB4 in the clearance of apoptotic cells in Drosophila, particularly in phagosome maturation, possibly through binding to apoptotic corpses to engage a phagosome maturation program dedicated to efferocytosis.
Article
Cardiac & Cardiovascular Systems
Daile Jia, Siqin Chen, Peiyuan Bai, Chentao Luo, Jin Liu, Aijun Sun, Junbo Ge
Summary: This study reveals the importance of cardiac resident macrophages in the efficient clearance and degradation of apoptotic cardiomyocytes during myocardial infarction, which is crucial for inflammation resolution and tissue repair. The researchers identified Lgmn as a key gene involved in this process and demonstrated its functional significance through various experiments.
Article
Cell Biology
Adele Kerjouan, Cyril Boyault, Christiane Oddou, Edwige Hiriart-Bryant, Alexei Grichine, Alexandra Kraut, Mylene Pezet, Martial Balland, Eva Faurobert, Isabelle Bonnet, Yohann Coute, Bertrand Fourcade, Corinne Albiges-Rizo, Olivier Destaing
Summary: Researchers developed an optogenetic probe to control SRC signaling, demonstrating its ability to generate different acto-adhesive structures by manipulating SRC kinase activity. Through proteomic and network analyses, they revealed the characteristics of distinct downstream signaling pathways regulated by optoSRC dynamics.
JOURNAL OF CELL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Matthew Jessop, Clarissa Liesche, Jan Felix, Ambroise Desfosses, Megghane Baulard, Virgile Adam, Angelique Fraudeau, Karine Huard, Gregory Effantin, Jean-Philippe Kleman, Maria Bacia-Verloop, Dominique Bourgeois, Irina Gutsche
Summary: This study investigates the acid stress-response regulation of Escherichia coli LdcI through a combination of biochemical and biophysical characterization, negative stain and cryo-electron microscopy, and fluorescence imaging. The research reveals that LdcI forms distinct patches at the cell periphery and assembles into filaments at physiologically relevant low pH, with structures of these filaments and the LdcI decamer being solved by cryo-EM. The findings provide insights into the supramolecular organization of LdcI and its role in the acid stress response.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Julien Aureille, Mylene Pezet, Lydia Pernet, Jacques Mazzega, Alexei Grichine, Christophe Guilluy, Monika Elzbieta Dolega
Summary: Deciphering how individual cells process and respond to signals from the environment is a central challenge in developmental biology and disease understanding. Recent genomic studies have provided information about population heterogeneity, but spatial heterogeneity remains poorly linked with mechanical and biochemical cues. A new method based on the use of photo convertible proteins has been developed to study genetic alterations and spatial distribution of cells in response to environmental signals.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Biochemistry & Molecular Biology
Catherine Wicker-Planquart, Pascale Tacnet-Delorme, Laurence Preisser, Samy Dufour, Yves Delneste, Dominique Housset, Philippe Frachet, Nicole M. Thielens
Summary: SREC-II, a membrane protein encoded by SCARF2 gene, has high homology with SR-F1 and can interact with ligands such as MalBSA. The similarities and differences in ligand binding properties of soluble SREC-II and SREC-II at the cell surface are highlighted in this study.
Article
Cell Biology
Slawomir Blonski, Julien Aureille, Sara Badawi, Damian Zaremba, Lydia Pernet, Alexei Grichine, Sandrine Fraboulet, Piotr M. Korczyk, Pierre Recho, Christophe Guilluy, Monika E. Dolega
Summary: Cell shape dynamics during development are tightly regulated and coordinated with cell fate determination. The interplay between biochemical and mechanical signals triggers complex tissue formation through coordinated cell shape changes. Signaling gradients associated with folding control spatially distinct biochemical responses within the monolayer.
DEVELOPMENTAL CELL
(2021)
Article
Chemistry, Applied
Simon Pascal, San-Hui Chi, Alexei Grichine, Veronique Martel-Frachet, Joseph W. Perry, Olivier Maury, Chantal Andraud
Summary: This study reports the synthesis and optical properties investigation of amino-heptamethines, providing new insights into the two-photon absorption of this type of chromophores and exploring their potential for bio-imaging of living cells.
Article
Multidisciplinary Sciences
Jan Felix, Ladislav Bumba, Clarissa Liesche, Angelique Fraudeau, Fabrice Rebeille, Jessica Y. El Khoury, Karine Huard, Benoit Gallet, Christine Moriscot, Jean-Philippe Kleman, Yoan Duhoo, Matthew Jessop, Eaazhisai Kandiah, Frederic Barras, Juliette Jouhet, Irina Gutsche
Summary: The membrane lipid composition is a key factor for bacterial stress adaptation. The ravA-viaA pathway responds to aminoglycosides under anaerobic conditions and is involved in cell membrane regulation.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Inorganic & Nuclear
Ji-Hyung Choi, Guillaume Fremy, Thibault Charnay, Nour Fayad, Jacques Pecaut, Sule Erbek, Niko Hildebrandt, Veronique Martel-Frachet, Alexei Grichine, Olivier Seneque
Summary: In this article, a novel microscopy imaging probe based on lanthanide complexes is described. The probe exhibits desirable luminescence properties and cell-penetrating ability for 2P imaging of live cells. By conjugating the lanthanide complexes with cell-penetrating peptides, successful synthesis of the conjugates was achieved and their application in various microscopy imaging techniques was evaluated. The results demonstrate that the probe has proper internalization and cytosolic delivery in cells, while maintaining its original luminescence properties. These findings provide new insights for the development of functional probes for live cell microscopy imaging.
INORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Kimi Azad, Delphine Guilligay, Cecile Boscheron, Sourav Maity, Nicola De Franceschi, Guidenn Sulbaran, Gregory Effantin, Haiyan Wang, Jean-Philippe Kleman, Patricia Bassereau, Guy Schoehn, Wouter H. Roos, Ambroise Desfosses, Winfried Weissenhorn
Summary: This study presents cryo-EM structures of membrane-coated CHMP2A-CHMP3 filaments from Homo sapiens, which reveal helical filaments assembled by CHMP2A-CHMP3 heterodimers in the open ESCRT-III conformation. Inter-filament interactions are electrostatic, facilitating filament sliding upon VPS4-mediated polymer remodeling. Fluorescence microscopy and high-speed atomic force microscopy imaging confirm that VPS4 can constrict and cleave CHMP2A-CHMP3 membrane tubes. It is concluded that CHMP2A-CHMP3-VPS4 act as a minimal membrane fission machinery.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Solene Besson, David Laurin, Cyrielle Chauviere, Michel Thepaut, Jean-Philippe Kleman, Mylene Pezet, Olivier Manches, Franck Fieschi, Caroline Aspord, Pascal Fender
Summary: Virus-like particles can serve as versatile vectors for vaccine platforms in various fields. This study presents a newly designed virus-like particle and investigates its immunogenicity and impact on human dendritic cell subsets. The results demonstrate that the virus-like particles induce strong immune stimulation and effectively activate antigen-specific T cell responses. By engineering the platform structure, it is possible to enhance targeting and broaden immune responses, laying the foundation for its potential utilization as an off-the-shelf vaccine.
Article
Biology
Samy Dufour, Pascale Tacnet-Delorme, Jean-Philippe Kleman, Oleksandr Glushonkov, Nicole Thielens, Dominique Bourgeois, Philippe Frachet
Summary: CD47 serves as a "don't eat-me" signal to protect viable cells from phagocytosis by macrophages. Apoptosis induces changes in the plasma membrane, which leads to exposure of "eat-me" signals such as phosphatidylserine and calreticulin. This study uses imaging techniques to investigate how the distribution of these molecules on the cell surface correlates with plasma membrane alteration, SIRP alpha binding, and cell engulfment.
COMMUNICATIONS BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Guillermo A. Orsi, Maxime M. C. Tortora, Beatrice Horard, Dominique Baas, Jean-Philippe Kleman, Jonas Bucevicius, Grazvydas Lukinavicius, Daniel Jost, Benjamin Loppin
NATURE COMMUNICATIONS
(2023)
Article
Materials Science, Multidisciplinary
Silvia Mizzoni, Silvia Ruggieri, Annika Sickinger, Francois Riobe, Laure Guy, Margaux Roux, Guillaume Micouin, Akos Banyasz, Olivier Maury, Bruno Baguenard, Amina Bensalah-Ledoux, Stephan Guy, Alexei Grichine, Xuan-Nhi Nguyen, Andrea Cimarelli, Martina Sanadar, Andrea Melchior, Fabio Piccinelli
Summary: In this study, two pairs of cationic enantiomeric complexes were synthesized and characterized. They showed good sensitization of Sm(iii) and Eu(iii) luminescence and exhibited high CPL activity. In cell experiments, these complexes showed promise as nanoscale bioprobes.
JOURNAL OF MATERIALS CHEMISTRY C
(2023)
Article
Multidisciplinary Sciences
Massilia Abbas, Meriem Maalej, Ferran Nieto-Fabregat, Michel Thepaut, Jean-Philippe Kleman, Isabel Ayala, Antonio Molinaro, Jean-Pierre Simorre, Roberta Marchetti, Franck Fieschi, Cedric Laguri
Summary: Lipopolysaccharides, a characteristic of gram-negative bacteria, play a crucial role in bacterial integrity. The immune system recognizes lipopolysaccharides through C-type lectin receptors. A study on macrophage galactose lectin reveals a unique structure that allows high-affinity binding to lipopolysaccharides on the bacterial surface.