期刊
JOURNAL OF INNATE IMMUNITY
卷 2, 期 5, 页码 483-494出版社
KARGER
DOI: 10.1159/000316574
关键词
Regulatory B cells; Pattern recognition; Toll-like receptors; CpG oligodeoxynucleotides; Protein kinases; Kinome analysis; Peyer's patches
类别
资金
- Natural Science and Engineering Research Council
- National Institutes of Health and Merial Ltd.
Intestinal Peyer's patch (PP) regulatory CD21+ B cells (B-regs) suppress TLR9-induced innate immune responses. However, it is not known whether TLR9 activation is regulated in PP B-regs. Here, we investigated the responses of PP B-regs to Stimulation with the TLR9 agonist CpG oligodeoxynucleotides (ODN). We observed that PP CD21+ B-regs express high levels of TLR9 mRNA, but fail to proliferate when stimulated with CpG ODN. Furthermore, unlike CD21+ B cells from blood, PP CD21+ B-regs do not secrete IgM or IL-12 following CpG ODN stimulation. We hypothesized that the unresponsiveness of PP B-regs to CpG stimulation was due to an inability of the TLR9 agonist to activate the TLR9 signaling pathway in these cells. This was confirmed by kinome analysis which demonstrated dynamic patterns of phosphorylation of key TLR adaptor proteins such as IRAK1, TAK1, IKK and NF-kappa B-p65 in CpG-stimulated blood CD21+ B cells, consistent with activation of the TLR9 pathway. In contrast, stimulation of PP CD21+ B-regs with CpG ODN resulted in phosphorylation patterns of these adaptor proteins suggestive of inactivation of the TLR9 pathway. The absence of apparent TLR9 signaling events immediately following stimulation indicated that signaling is blocked close to the receptor. Our observations suggest a novel mechanism by which the host regulates TLR responses in TLR-expressing cells with regulatory functions. (C) Copyright 2010 S. Karger AG, Basel
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