期刊
JOURNAL OF INFECTIOUS DISEASES
卷 210, 期 1, 页码 89-98出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiu002
关键词
miR-155; Pseudomonas aeruginosa; corneal infection; phagocytosis; bacterial killing; inflammation; Rheb
资金
- National Natural Science Foundation of China [U0832006, 81261160323, 31200662, 31370868, 81172811]
- State Key Laboratory of Ophthalmology (Zhongshan Ophthalmic Center)
- Guangdong Innovative Research Team Program [2009010058, 2011Y035]
- Doctoral Program of Higher Education of China [20100171110047, 20120171120064]
- Guangdong Natural Science Foundation [10251008901000013, S2012040006680]
- 111 Project [B13037]
- Guangdong Province Universities and Colleges
- National Science and Technology Key Projects for Major Infectious Diseases [2013ZX10003001]
- State Key Laboratory of Ophthalmology
miR-155 (microRNA-155) is an important noncoding RNA in regulating host inflammatory responses. However, its regulatory role in ocular infection remains unclear. Our study first explored the function of miR-155 in Pseudomonas aeruginosa-induced keratitis, one of the most common sight-threatening ocular diseases. We found that miR-155 expression was enhanced in human and mouse corneas after P. aeruginosa infection and was mainly expressed in macrophages but not neutrophils. In vivo studies demonstrated that miR-155 knockout mice displayed more resistance to P. aeruginosa keratitis, with a higher inducible nitric oxide synthase level and a lower bacterial burden. More importantly, in vitro data indicated that miR-155 suppressed the macrophage-mediated bacterial phagocytosis and intracellular killing of P. aeruginosa by targeting Rheb (Ras homolog enriched in brain). To the best of our knowledge, this is the first study to explore the role of miR-155 in bacterial keratitis, which may provide a promising target for clinical treatment of P. aeruginosa keratitis and other infectious diseases.
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