期刊
JOURNAL OF INFECTIOUS DISEASES
卷 210, 期 2, 页码 200-208出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiu085
关键词
rabies; post-exposure prophylaxis; RIG; monoclonal antibody; Nicotiana tabacum
资金
- EU
- Wellcome Trust
- Hotung Foundation
- Dr Hadwen Trust
- St George's, University of London, UK
- AHVLA, Weybridge, UK
Rabies post-exposure prophylaxis (PEP) currently comprises administration of rabies vaccine together with rabies immunoglobulin (RIG) of either equine or human origin. In the developing world, RIG preparations are expensive, often in short supply, and of variable efficacy. Therefore, we are seeking to develop a monoclonal antibody cocktail to replace RIG. Here, we describe the cloning, engineering and production in plants of a candidate monoclonal antibody (E559) for inclusion in such a cocktail. The murine constant domains of E559 were replaced with human IgG1 kappa constant domains and the resulting chimeric mouse-human genes were cloned into plant expression vectors for stable nuclear transformation of Nicotiana tabacum. The plant-expressed, chimeric antibody was purified and biochemically characterized, was demonstrated to neutralize rabies virus in a fluorescent antibody virus neutralization assay, and conferred protection in a hamster challenge model.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据