Article
Microbiology
Oren Gordon, Dustin A. Dikeman, Roger Ortines, Yu Wang, Christine Youn, Mohammed Mumtaz, Nicholas Orlando, Jeffrey Zhang, Aman Patel, Ethan Gough, Amit Kaushik, Eric L. Nuermberger, Anna M. Upton, Nader Fotouhi, Lloyd S. Miller, Nathan K. Archer
Summary: Staphylococcus aureus is a major cause of bloodstream, skin, and bone infections in humans. The emergence of drug-resistant strains increases the difficulty in treating these infections. This study compared the efficacy of a novel oxazolidinone antibiotic, TBI-223, with linezolid in mouse models of MRSA infection. TBI-223 showed comparable efficacy to linezolid in reducing bacterial burden and disease severity in all three models.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Tong Yi, Wenxin Ding, Yuanzhen Hao, Lifeng Cen, Jiyang Li, Xunlong Shi, Ting Wang, Daofeng Chen, Haiyan Zhu
Summary: Influenza virus and bacterial co-infection contribute to severe inflammation and acute lung injury through excessive neutrophil recruitment and formation of neutrophil extracellular traps (NETs). Treatment with DNase I or a selective neutrophil elastase inhibitor (NEi) can alleviate the severity of lung injury.
MICROBIAL PATHOGENESIS
(2022)
Article
Immunology
Rosanna Leuzzi, Margherita Bodini, Isaac P. Thomsen, Elisabetta Soldaini, Erika Bartolini, Alessandro Muzzi, Bruna Clemente, Bruno Galletti, Andrea Guido Oreste Manetti, Cinzia Giovani, Stefano Censini, Sonia Budroni, Fabiana Spensieri, Erica Borgogni, Silvia Rossi Paccani, Immaculada Margarit, Fabio Bagnoli, Giuseppe Del Giudice, Clarence B. Creech
Summary: The study identified specific cytokine and functional antibody signatures in patients with different primary invasive diseases caused by Staphylococcus aureus. These data provide insights into human responses to invasive staphylococcal infections and are important for guiding the identification of novel preventive and therapeutic interventions against S. aureus. Our findings showed distinct differences in inflammatory responses and antibody levels between patients with invasive S. aureus disease and healthy donors.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Infectious Diseases
Catia Cilloniz, Cristina Dominedo, Albert Gabarrus, Carolina Garcia-Vidal, Jose Becerril, Diego Tovar, Estela Moreno, Juan M. Pericas, Carmen Rosa Vargas, Antoni Torres
Summary: This study aimed to describe the prevalence, risk factors, and outcomes of MSSA community-acquired pneumonia and found that MSSA CAP patients had higher 30-day mortality than pneumococcal CAP patients, with MSSA being an independent factor of mortality.
JOURNAL OF INFECTION
(2021)
Review
Immunology
Valentina Granata, Valentina Possetti, Raffaella Parente, Barbara Bottazzi, Antonio Inforzato, Cristina Sobacchi
Summary: Osteomyelitis is an infectious disease caused by Staphylococcus aureus, and it has a complex pathogenesis involving interactions between bone cells and immune cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Infectious Diseases
Harshad Lade, Hwang-Soo Joo, Jae-Seok Kim
Summary: MRSA is a drug-resistant pathogen with the ability to resist multiple antibiotics. It utilizes various resistance mechanisms to evade antibiotic action. This review discusses the molecular basis of MRSA resistance to non-beta-lactam antibiotics, providing insights for the development of new treatment approaches and detection methods for antibiotic resistance.
Review
Biochemistry & Molecular Biology
Pietro Speziale, Giampiero Pietrocola
Summary: NETs are essential components of the innate immune system during bacterial infections. Staphylococcus aureus can evade NET-mediated killing through various mechanisms, such as degrading NETs and inhibiting their formation. Understanding the virulence factors involved in NET formation/neutralization could lead to potential therapeutic interventions against this pathogen.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Multidisciplinary Sciences
Xinhai Chen, Haley Gula, Tonu Pius, Chong Ou, Margaryta Gomozkova, Lai-Xi Wang, Olaf Schneewind, Dominique Missiakas
Summary: This study reveals that the clearance of Staphylococcus aureus is achieved through different mechanisms mediated by different subclasses of antibodies. FcγRIV is the main protective pathway for antibody 3F6-mIgG2a in C57BL/6J mice, while CR3 serves as the main pathway in BALB/cJ mice. These findings may have clinical implications in predicting the efficacy of antibody therapies and could be relevant in other mammals including humans.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Immunology
Colleen S. Curran, Lindsay M. Busch, Yan Li, Cui Xizhong, Junfeng Sun, Peter Q. Eichacker, Parizad Torabi-Parizi
Summary: In this study, it was found that anti-PD-L1 therapy did not improve survival in pneumonia models in mice, suggesting the need for further preclinical studies on other common pathogens and septic foci.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Immunology
Tiziano A. Schweizer, Federica Andreoni, Claudio Acevedo, Thomas C. Scheier, Irina Heggli, Ewerton Marques Maggio, Nadia Eberhard, Silvio D. Brugger, Stefan Dudli, Annelies S. Zinkernagel
Summary: This study investigates the pathophysiology of Staphylococcus aureus-induced spondylodiscitis, and provides insights into the role of inflammatory intervertebral disc (IVD) cell death and potential therapeutic approaches. Clinical data and an ex vivo porcine model showed that S. aureus infection leads to IVD cell death, release of inflammatory markers, and recruitment of neutrophils.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Mariane Pivard, Isabelle Caldelari, Virginie Brun, Delphine Croisier, Michel Jaquinod, Nelson Anzala, Benoit Gilquin, Chloe Teixeira, Yvonne Benito, Florence Couzon, Pascale Romby, Karen Moreau, Francois Vandenesch
Summary: The study found that there are significant variations in the production of Staphylococcus aureus HlgCB protein in patients with pneumonia, as well as inconsistent levels of HlgC and HlgB in certain strains. Analysis of clinical isolates and reference strains revealed that the levels of HlgCB and the HlgC/HlgB ratio depend on the activity of the hlgC promoter, mRNA processing, and translation. Additionally, a single nucleotide polymorphism (SNP) in the 5' untranslated region (UTR) of hlgCB mRNA was found to inhibit the translation of HlgC, leading to a decrease in HlgCB levels.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Zhen-Zhen Liu, Yong-Jun Yang, Cheng-Kai Zhou, Shi-Qing Yan, Ke Ma, Yu Gao, Wei Chen
Summary: STING plays a crucial role in suppressing necroptosis to combat pulmonary S. aureus infection, with STING deficiency resulting in impaired bacterial clearance and increased cell death.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Infectious Diseases
Helio S. Sader, Leonard R. Duncan, Rodrigo E. Mendes
Summary: The study evaluated the activities of dalbavancin against Staphylococcus aureus isolated from the lower respiratory tract of both cystic fibrosis (CF) and non-CF patients, showing high potency and complete coverage against isolates from both groups.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Pharmacology & Pharmacy
Narayan Pant, Socorro Miranda-Hernandez, Catherine Rush, Jeffrey Warner, Damon P. Eisen
Summary: The study demonstrates that Ticagrelor shows antibacterial and antibiofilm activity against Staphylococcus aureus in vitro, with a strain-specific downregulation effect on biofilm-related genes. In an animal model, Ticagrelor combined with antibiotics significantly reduces bacterial concentrations on implants and decreases bacterial dissemination to surrounding tissues.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Immunology
Maren von Koeckritz-Blickwede, Volker Winstel
Summary: NETosis is a cellular process that promotes the formation of neutrophil extracellular traps (NETs). However, pathogens often take advantage of NETs to cause persistent infections. Staphylococcus aureus, a high-priority pathogen, catalyzes and exploits NETs during pathogenesis and recurrent infections. This article focuses on how staphylococci induce NET formation and highlights the molecular and underlying principles of NETosis. It also discusses the inflammatory potential of NET-controlled microenvironments and the adaptation and counteracting strategies evolved by S. aureus to impede NET-mediated killing.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alexis Broquet, Anissa Besbes, Jerome Martin, Cedric Jacqueline, Mickael Vourc'h, Antoine Roquilly, Jocelyne Caillon, Regis Josien, Karim Asehnoune
MOLECULAR IMMUNOLOGY
(2020)
Article
Immunology
Antoine Roquilly, Cedric Jacqueline, Marion Davieau, Alice Molle, Abderrahmane Sadek, Cynthia Fourgeux, Paul Rooze, Alexis Broquet, Barbara Misme-Aucouturier, Tanguy Chaumette, Mickael Vourc'h, Raphael Cinotti, Nadege Marec, Vanessa Gauttier, Hamish E. G. McWilliam, Frederic Altare, Jeremie Poschmann, Jose A. Villadangos, Karim Asehnoune
Article
Immunology
Benjamin J. Gaborit, Antoine Roquilly, Cedric Louvet, Abderrahmane Sadek, Benoit Tessoulin, Alexis Broquet, Cedric Jacqueline, Mickael Vourc'h, Tanguy Chaumette, Marie Chauveau, Antoine Asquier, Alexandre Bourdiol, Virginie Le Mabecque, Marion Davieau, Jocelyne Caillon, David Boutoille, Fanny Coulpier, Sophie Lemoine, Emilie Ronin, Jeremie Poschmann, Benoit L. Salomon, Karim Asehnoune
JOURNAL OF INFECTIOUS DISEASES
(2020)
Correction
Immunology
Antoine Roquilly, Cedric Jacqueline, Marion Davieau, Alice Molle, Abderrahmane Sadek, Cynthia Fourgeux, Paul Rooze, Alexis Broquet, Barbara Misme-Aucouturier, Tanguy Chaumette, Mickael Vourc'h, Raphael Cinotti, Nadege Marec, Vanessa Gauttier, Hamish E. G. McWilliam, Frederic Altare, Jeremie Poschmann, Jose A. Villadangos, Karim Asehnoune
Article
Immunology
Mickael Vourc'h, Gaelle David, Benjamin Gaborit, Alexis Broquet, Cedric Jacqueline, Tanguy Chaumette, Jocelyne Caillon, Antoine Roquilly, Christelle Retiere, Karim Asehnoune
INFECTION AND IMMUNITY
(2020)
Article
Behavioral Sciences
Ruby A. Natale, Folefac Atem, Sitara Weerakoon, Cynthia Lebron, M. Sunil Mathew, Krystal Sardinas, Catherina Chang, Karla P. Shelnutt, Rachel Spector, Fiorella Altare, Sarah E. Messiah
Summary: This study compared the effectiveness of HC2 phase 1 and phase 2 obesity prevention interventions on child dietary practices and PBMI outcomes over 2 years in 24 CCCs. Results showed different effects between the two phases, with increases in fruit and vegetable intake and decreases in unhealthy food consumption in phase 1, while a decrease in snack consumption in phase 2, with all groups maintaining healthy PBMI over the two years.
JOURNAL OF DEVELOPMENTAL AND BEHAVIORAL PEDIATRICS
(2021)
Article
Infectious Diseases
Lise Cremet, Anne-Gaelle Leroy, Damien Muller, Sandie Delanou, Alexandra Burghelea, Alexis Broquet, Antoine Roquilly, Nathalie Caroff
Summary: This study revealed the within-host heterogeneity of Pseudomonas aeruginosa populations from pneumonia by examining colonies from bronchoalveolar lavage (BAL), showing differences in antibiotic susceptibility and quorum-sensing (QS) phenotypes. It also highlighted a significant association between QS deficiency and ceftazidime resistance, emphasizing the importance of examining diverse colonies when analyzing BAL cultures.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Biochemistry & Molecular Biology
Clarisse Roblin, Steve Chiumento, Cedric Jacqueline, Eric Pinloche, Cendrine Nicoletti, Hamza Olleik, Elise Courvoisier-Dezord, Agnes Amouric, Christian Basset, Louis Dru, Marie Ollivier, Aurelie Bogey-Lambert, Nicolas Vidal, Mohamed Atta, Marc Maresca, Estelle Devillard, Victor Duarte, Josette Perrier, Mickael Lafond
Summary: The research indicates that ruminococcin C1, a RiPPs molecule, has strong antibacterial activity and safety, effectively treating infections and protecting challenged mice from resistant bacteria. It selectively impacts the gut microbial community while maintaining global microbiome homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Cedric Jacqueline, Jocelyne Caillon, Olivier Meyer, Eric Dailly, Carl Simonsson, Vincent Lenaerts, Karim Asehnoune, Amokrane Reghal, Gilles Potel
Summary: Staphylococcus aureus bone infections present a therapeutic challenge, but local nanoparticle-encapsulated daptomycin shows great potential in efficiently sterilizing infectious sites.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Medicine, Research & Experimental
Sothea Touch, Emmanuelle Godefroy, Nathalie Rolhion, Camille Danne, Cyriane Oeuvray, Marjolene Straube, Chloe Galbert, Loic Brot, Iria Alonso Salgueiro, Sead Chadi, Tatiana Ledent, Jean-Marc Chatel, Philippe Langella, Francine Jotereau, Frederic Altare, Harry Sokol
Summary: The abundance of Faecalibacterium prausnitzii is decreased in patients with inflammatory bowel diseases (IBD). This study found that DP8 alpha Treg cells were reduced in IBD patients. Using a humanized mouse model, researchers demonstrated a protective effect of a HLA-DR*0401-restricted DP8 alpha Treg clone combined with F. prausnitzii administration in a colitis model. The frequency of circulating DP8 alpha cells was found to be independently associated with disease activity in IBD patients. Furthermore, a positive correlation was found between F. prausnitzii-specific DP8 alpha Tregs and the amount of F. prausnitzii in fecal microbiota in healthy individuals and patients with ileal Crohn's disease.
Meeting Abstract
Hematology
Emmanuelle Godefroy, Patrice Chevallier, Fabienne Haspot, Caroline Vignes, Veronique Daguin, Sylvia Lambot, Thierry Guillaume, Margaux Verdon, Pierre Peterlin, Alice Garnier, Maxence Mougon, Amandine Le Bourgeois, Maxime Jullien, Francine Jotereau, Frederic Altare
Article
Immunology
Francine Jotereau, Joudy Alameddine, Raluca Teusan, Annabelle Pedron, Nicolas Jouand, Frederic Altare, Emmanuelle Godefroy
Summary: In this study, a unique subset of Tregs called DP8 alpha Tregs was identified in humans. These Tregs secrete IL-10, co-express CD4 and CD8 alpha, and react against the symbiotic bacterium Faecalibacterium prausnitzii. They exhibit a mixed Tr1-like/cytotoxic CD4(+) T cell-profile and share master genes with mouse gut microbiota-induced Tregs. DP8 alpha Tregs possess potent cytotoxic, chemotactic, and IgA-promoting abilities. The discovery of their complete signature and novel functional properties provides important insights into their physiological roles and clinical applications in inflammatory bowel diseases.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Remy Char, Zhuangzhuang Liu, Cedric Jacqueline, Marion Davieau, Maria-Graciela Delgado, Clara Soufflet, Mathieu Fallet, Lionel Chasson, Raphael Chapuy, Voahirana Camosseto, Eva Strock, Rejane Rua, Catarina R. Almeida, Bing Su, Ana-Maria Lennon-Dumenil, Beatrice Nal, Antoine Roquilly, Yinming Liang, Stephane Meresse, Evelina Gatti, Philippe Pierre
Summary: This study shows that iRUFY3 plays a role in perinuclear positioning, phagocyte migration and antigen presentation during Salmonella infection. iRUFY3 controls macrophages migration, MHC II presentation and responses to Interferon-γ, while being important for intracellular Salmonella replication.
NATURE COMMUNICATIONS
(2023)
Meeting Abstract
Hematology
Emmanuelle Godefroy, Patrice Chevallier, Thierry Guillaume, Pierre Peterlin, Alice Garnier, Amandine Le Bourgeois, Annabelle Pedron, Frederic Altare, Francine Jotereau