Article
Immunology
Lorenza Pugni, Carlo Pietrasanta, Andrea Ronchi, Giovanna Lunghi, Laura Pellegrinelli, Fabio Mosca, Giulia Campanini, Fausto Baldanti
Summary: HHV-6 can integrate its genome in human chromosomes and be inherited by the next generation. Diagnosing inherited chromosomally integrated HHV-6 in neonates is crucial to avoid misdiagnosis and unnecessary treatment.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2021)
Article
Microbiology
Liliana Gabrielli, Alice Balboni, Eva Caterina Borgatti, Giulio Virgili, Evangelia Petrisli, Alessia Cantiani, Matteo Pavoni, Federico Baiesi Pillastrini, Simona Venturoli, Giulia Piccirilli, Tiziana Lazzarotto
Summary: This study aimed to determine the prevalence of inherited chromosomally integrated human herpesvirus 6 (iciHHV-6) in hospitalized patients and investigate the clinical features of individuals carrying this integration. HHV-6 PCR on hair follicles was used to confirm iciHHV-6 status when the blood viral load was more than 5 Log(10) copies/mL. The results showed that the prevalence of iciHHV-6 in our population was 2.6% (53/2019).
Article
Immunology
Hiroki Miura, Yoshiki Kawamura, Tamae Ohye, Fumihiko Hattori, Kei Kozawa, Masaru Ihira, Hiroshi Yatsuya, Haruki Nishizawa, Hiroki Kurahashi, Tetsushi Yoshikawa
Summary: Inherited chromosomally integrated HHV-6 (iciHHV-6) can be transmitted from parent to child and is associated with increased risk of spontaneous abortion, especially in mothers who are iciHHV-6 positive and those who are ≥40 years old at their most recent pregnancy.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Immunology
Michael Mariani, Cosima Zimmerman, Princess Rodriguez, Ellie Hasenohr, Giulia Aimola, Diana Lea Gerrard, Alyssa Richman, Andrea Dest, Louis Flamand, Benedikt Kaufer, Seth Frietze
Summary: Human herpesvirus HHV-6A/B can integrate their genomes into human chromosomes, leading to individuals harboring the viral genome in every cell. Research shows that HHV-6A becomes transcriptionally silent in infected cells and interacts with quiescent chromatin states. Most virus chromatin interactions occur towards the distal ends of specific human chromosomes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Hematology
Madeleine R. Heldman, Cassandra Job, Joyce Maalouf, Jessica Morris, Hu Xie, Chris Davis, Terry Stevens-Ayers, Meei-Li Huang, Keith R. Jerome, Jesse R. Fann, Danielle M. Zerr, Michael Boeckh, Joshua A. Hill
Summary: This study aimed to compare the incidence of CNS symptoms after allogeneic HCT among patients with iciHHV-6(pos) and iciHHV-6(neg), and assess the impact of HHV-6 detection on unnecessary interventions. The cumulative incidence of CNS symptoms was similar between iciHHV-6(pos) and iciHHV-6(neg) HCT recipients, but HHV-6 detection in iciHHV-6(pos) patients led to more frequent antiviral therapy and unnecessary lumbar punctures. Testing for iciHHV-6 may improve patient management after allogeneic HCT.
TRANSPLANTATION AND CELLULAR THERAPY
(2021)
Article
Immunology
Eleanor C. Semmes, Shuk Hang Li, Jillian H. Hurst, Zidanyue Yang, Donna Niedzwiecki, Genevieve G. Fouda, Joanne Kurtzberg, Kyle M. Walsh, Sallie R. Permar
Summary: The study found that congenital HCMV infection moderately reduces transplacental IgG transfer efficiency due to maternal hypergammaglobulinemia; however, infants with and without cCMV infection had similar antigen-specific IgG levels acquired from maternal IgG via transplacental transfer.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Seiichiro Katagiri, Daigo Akahane, Tatsuya Inukai, Shunsuke Otsuki, Arisa Yamada, Mitsuru Moriyama, Akiko Yamada, Michiyo Asano, Seiichiro Yoshizawa, Yuko Tanaka, Nahoko Furuya, Hiroaki Fujimoto, Moritaka Gotoh, Shigeki Nakamura, Akihiko Gotoh
Summary: This case report focuses on the behavior of HHV-6 in a patient with ciHHV-6 who underwent two CBTs. Changes in HHV-6 DNA levels in peripheral blood leukocytes during the disease course were observed, suggesting the potential release of HHV-6 genome due to tissue damage. Physicians should be aware of the variability of HHV-6 DNA during allogeneic hematopoietic stem cell transplantation in ciHHV-6 patients.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2021)
Article
Virology
Sandra Cadena-Mota, Juan C. Munoz-Escalante, Luz E. Martinez-Rodriguez, Sofia Bernal-Silva, Nallely Tello-Martinez, Ileana de la Torre-Rodriguez, Pedro G. Hernandez-Sanchez, Fernanda Castillo-Martinez, Francisco Escalante-Padron, Victoria Lima-Rogel, Ana M. Gonzalez-Ortiz, Daniel E. Noyola
Summary: Cytomegalovirus infection is common in preterm infants, with a high incidence of congenital and postnatal infection. Breastfeeding duration is associated with the risk of infection, and different cytomegalovirus strains are distributed among the infants.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Review
Immunology
Anthony L. Komaroff, Roberta Rizzo, Jeffrey L. Ecker
Summary: HHV-6A and HHV-6B are ancient human viruses that can cause lifelong infection and are associated with various diseases. These viruses may have an impact on infertility, preeclampsia, and other reproductive diseases, but further research is needed to fully understand their role.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Laure Izquierdo, Clemence M. Canivet, Eleonora De Martin, Teresa M. Antonini, Anne-Marie Roque-Afonso, Audrey Coilly, Claire Deback
Summary: This article reports a case of a patient with iciHHV-6A and iciHHV-6B who developed acute fulminant hepatic failure after using ulipristal acetate, and the Mendelian inheritance of the virus was confirmed through family studies. Due to the rarity of this presentation, the possible links between iciHHV-6 and adverse drug reactions were discussed, and it was suggested to screen for iciHHV-6 before using hepatotoxic drugs.
Article
Microbiology
Irina Anatolyevna Andrievskaya, Irina Valentinovna Zhukovets, Inna Victorovna Dovzhikova, Nataliya Alexandrovna Ishutina, Ksenia Konstantinovna Petrova
Summary: The research aims to evaluate HSV-1 seropositivity among pregnant women and its impact on pregnancy, childbirth, and newborns. The findings suggest that different titers of HSV-1 antibodies during different trimesters are associated with various complications such as threatened miscarriage, anemia, and placental insufficiency.
Article
Biochemistry & Molecular Biology
Amr Aswad, Giulia Aimola, Darren Wight, Pavitra Roychoudhury, Cosima Zimmermann, Joshua Hill, Dirk Lassner, Hong Xie, Meei-Li Huang, Nicholas F. Parrish, Heinz-Peter Schultheiss, Cristina Venturini, Susanne Lager, Gordon C. S. Smith, D. Stephen Charnock-Jones, Judith Breuer, Alexander L. Greninger, Benedikt B. Kaufer
Summary: This study used advanced sequencing technology and mining of public human genome datasets to comprehensively understand the evolution of HHV-6, revealing that heritable integration of HHV-6 has existed since human migrations out of Africa. The research also showed that circulating and integrated HHV-6A and HHV-6B have distinct evolutionary histories and characteristics.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Pediatrics
Lisa Biswas, Noreen Crain, Michael C. Spaeder, Robert J. Gomez, Meghan Starolis, Melinda D. Poulter, Steven L. Zeichner
Summary: Multisystem inflammatory syndrome in children (MIS-C) is a serious late complication of COVID-19 with unknown pathogenesis and immune activation. Research has shown a potential association between MIS-C and the presence of HHV-6, suggesting a role in the pathogenesis of the disease.
Article
Multidisciplinary Sciences
Christy Hong, Michael Schubert, Andrea E. Tijhuis, Marta Requesens, Maurits Roorda, Anouk van den Brink, Lorena Andrade Ruiz, Petra L. Bakker, Tineke van der Sluis, Wietske Pieters, Mengting Chen, Rene Wardenaar, Bert van der Vegt, Diana C. J. Spierings, Marco de Bruyn, Marcel A. T. M. van Vugt, Floris Foijer
Summary: Chromosomal instability (CIN) drives cancer cell evolution, metastasis, and therapy resistance. This study reveals that the inactivation of cGAS-STING signaling selectively impairs the survival of triple-negative breast cancer cells with CIN. Furthermore, blockade of IL-6 signaling using tocilizumab selectively impairs the growth of triple-negative breast cancer cells with CIN.
Article
Microbiology
Michiko Shimoda, Tomoki Inagaki, Ryan R. Davis, Alexander Merleev, Clifford G. Tepper, Emanual Maverakis, Yoshihiro Izumiya
Summary: Kaposi's sarcoma-associated virus uses vIL-6 to activate STAT1 and 3, induce inflammatory gene expression, promote proliferation and differentiation of monocytes into macrophages, and maintain KSHV transcription, allowing the virus to escape from immune surveillance.
Article
Immunology
Annabelle Mouammine, Annie Gravel, Isabelle Dubuc, Yassamin Feroz Zada, Sylvie Provost, David Busseuil, Jean-Claude Tardif, Marie-Pierre Dube, Louis Flamand
JOURNAL OF INFECTIOUS DISEASES
(2020)
Article
Virology
Vikas Peddu, Isabelle Dubuc, Annie Gravel, Hong Xie, Meei-Li Huang, Dan Tenenbaum, Keith R. Jerome, Jean-Claude Tardif, Marie-Pierre Dube, Louis Flamand, Alexander L. Greninger
JOURNAL OF VIROLOGY
(2020)
Article
Microbiology
Vanessa Collin, Annie Gravel, Benedikt B. Kaufer, Louis Flamand
Article
Virology
Annie Gravel, Wes Sanders, Eric Fournier, Arnaud Droit, Nathaniel Moorman, Louis Flamand
Summary: This study utilized RNA sequencing to analyze new and differentially expressed transcripts during HHV-6B infection, revealing the diversity and complexity of the HHV-6B transcriptome. By analyzing different time points, the presence of differentially spliced genes and their relative expression over time was determined. The study proposed a new version of the HHV-6B Z29 GenBank annotated file to facilitate trace-back and correlation with previous studies.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Sara Artusi, Emanuela Ruggiero, Matteo Nadai, Beatrice Tosoni, Rosalba Perrone, Annalisa Ferino, Irene Zanin, Luigi Xodo, Louis Flamand, Sara N. Richter
Summary: TMPyP4, known to interact with G-quadruplexes, has been found to inhibit HSV-1 DNA replication by interacting with viral G-quadruplexes. The antiviral activity is independent of inhibiting virus DNA replication or entry, and involves trapping virus particles inside cytoplasmic vesicles through a unique mechanism.
Article
Immunology
Michael Mariani, Cosima Zimmerman, Princess Rodriguez, Ellie Hasenohr, Giulia Aimola, Diana Lea Gerrard, Alyssa Richman, Andrea Dest, Louis Flamand, Benedikt Kaufer, Seth Frietze
Summary: Human herpesvirus HHV-6A/B can integrate their genomes into human chromosomes, leading to individuals harboring the viral genome in every cell. Research shows that HHV-6A becomes transcriptionally silent in infected cells and interacts with quiescent chromatin states. Most virus chromatin interactions occur towards the distal ends of specific human chromosomes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Letter
Hematology
Younes Zaid, Fadila Guessous, Florian Puhm, Wail Elhamdani, Lamyae Chentoufi, Andrew Conway Morris, Amine Cheikh, Farid Jalali, Eric Boilard, Louis Flamand
Article
Biochemistry & Molecular Biology
Anne-Sophie Archambault, Younes Zaid, Volatiana Rakotoarivelo, Caroline Turcotte, Etienne Dore, Isabelle Dubuc, Cyril Martin, Olivier Flamand, Youssef Amar, Amine Cheikh, Hakima Fares, Amine El Hassani, Youssef Tijani, Andreanne Cote, Michel Laviolette, Eric Boilard, Louis Flamand, Nicolas Flamand
Summary: The study found that severe COVID-19 patients have increased fatty acids and inflammatory lipid mediators in bronchoalveolar lavages (BALs), including thromboxane, leukotrienes, and monohydroxylated 15-lipoxygenase metabolites. Additionally, specialized pro-resolving mediators such as lipoxin A(4) and D-series resolvins are also increased, indicating a lipid mediator storm in the lungs of severe COVID-19 patients.
Article
Allergy
Younes Zaid, Etienne Dore, Isabelle Dubuc, Anne-Sophie Archambault, Olivier Flamand, Michel Laviolette, Nicolas Flamand, Eric Boilard, Louis Flamand
Summary: The study aimed to analyze and compare the presence of cytokines and lipid mediators of inflammation in the plasma and bronchoalveolar lavage (BAL) fluids of patients with severe COVID-19. The results showed high concentrations of numerous cytokines, chemokines, and lipid mediators in the BAL fluid of patients with severe COVID-19, indicating that hyperinflammation in the lungs of these patients is fueled by excessive production of chemokines and eicosanoids.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Biology
Michael L. Wood, Colin D. Veal, Rita Neumann, Nicolas M. Suarez, Jenna Nichols, Andrei J. Parker, Diana Martin, Simon P. R. Romaine, Veryan Codd, Nilesh J. Samani, Adriaan A. Voors, Maciej Tomaszewski, Louis Flamand, Andrew J. Davison, Nicola J. Royle
Summary: Human herpesviruses 6A and 6B can transition between telomere-integrated and free virus forms, with mainly maternal transmission of acquired virus observed in families. Some healthy adults have shown telomere integration of acqHHV-6B in saliva, indicating latency. The excision of viral genome from telomeres in iciHHV-6B carriers is high and tissue-specific, with potential reactivation from circular extrachromosomal forms.
Review
Cell Biology
Florian Puhm, Louis Flamand, Eric Boilard
Summary: This article explores the potential of pEVs as valuable biomarkers in disease, with a special focus on their role as predictors of and contributors to COVID-19.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Neurosciences
Rayan Khaddaj-Mallat, Natija Aldib, Maxime Bernard, Anne-Sophie Paquette, Aymeric Ferreira, Sarah Lecordier, Armen Saghatelyan, Louis Flamand, Ayman ElAli
Summary: COVID-19, caused by SARS-CoV-2, induces vascular-mediated neurological disorders via elusive mechanisms. Brain pericytes respond to the exposure of S protein by increasing ACE2 expression and undergoing phenotypic changes, impacting vascular and immune regulatory functions which may contribute to brain damage.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Immunology
Isabelle Dubuc, Julien Prunier, Emile Lacasse, Annie Gravel, Florian Puhm, Isabelle Allaeys, Anne-Sophie Archambault, Leslie Gudimard, Rosaria Villano, Arnaud Droit, Nicolas Flamand, Eric Boilard, Louis Flamand
Summary: The study investigates the effects of SARS-CoV-2 infection on lung lipid mediators in K18-ACE2 transgenic mice. The results show that certain lipid mediators are significantly increased while others are reduced in response to the infection. In addition, the mice exhibit lung inflammation and blood clot formation. Transcriptomic and cytokine/chemokine analysis further support these findings.
FRONTIERS IN IMMUNOLOGY
(2022)
